Predicted mutation
evidence seq id position mutation annotation gene description
RA Annotation_U21_Trimmed_contig_4 75,126 T→C C31R (TGC→CGC)  rasttk_feature_annotation_tool=annotate_proteins_km er_v2 → Replication‑associated recombination protein RarA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Annotation_U21_Trimmed_contig_475,1260TC100.0% 1437.9 / NA 348C31R (TGC→CGC) rasttk_feature_annotation_tool=annotate_proteins_km er_v2Replication‑associated recombination protein RarA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (140/208);  total (140/208)

Only 100 of 348 total aligned reads displayed.

GTTTTTTTATTTACTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACTGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCACC  >  Annotation_U21_Trimmed_contig_4/74996‑75254
                                                                                                                                  |                                                                                                                                
gTTTTTTTATTTACTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTATCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTTGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGTCCGCAt                                                                                                                              >  1:1629165/1‑135 (MQ=255)
 tttttttATTTACTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATg                                                                                                                             <  1:1443208/135‑1 (MQ=255)
     tttatttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTTTTTGTCAACAGCATTTGGTCGGTCCAGGAAAAATTATCGACCGCATGGTTa                                                                                                                         >  1:674760/1‑135 (MQ=255)
     tttatttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTAGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTa                                                                                                                         >  1:2981304/1‑135 (MQ=255)
        atttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAATAAATTGTTGGTCAACAGAATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAg                                                                                                                      <  1:398600/135‑1 (MQ=255)
        atttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAg                                                                                                                      <  2:450996/135‑1 (MQ=255)
                   aTTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACtttt                                                                                                            >  2:339186/1‑134 (MQ=255)
                       ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                       <  2:1879043/135‑1 (MQ=255)
                       ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGACAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAACCTTTTatcat                                                                                                       <  1:1816390/135‑1 (MQ=255)
                       taattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                       <  2:2414641/133‑1 (MQ=255)
                            ttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATg                                                                                                  <  1:639932/135‑1 (MQ=255)
                                  gaAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAAATATCGAAGAAATTGTTGGTCAACAGCTTTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGAtttt                                                                                             <  1:2038126/134‑1 (MQ=255)
                                     aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                         <  1:2228722/135‑1 (MQ=255)
                                     aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                         <  2:1558897/135‑1 (MQ=255)
                                     aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                         <  1:1084945/135‑1 (MQ=255)
                                     aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                         <  1:1999898/135‑1 (MQ=255)
                                       aaTACAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACgg                                                                                       <  2:2955549/135‑1 (MQ=255)
                                          gCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTAAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGccc                                                                                    <  2:1940858/135‑1 (MQ=255)
                                               cATTTGCGTTTGGTATGCGCCAACGAACTTTCGTTGAATTTGTTTGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                               <  2:105625/135‑1 (MQ=255)
                                               cATTAGCTTTTCTTATGCGCCCACGAACTATCGAAGAAATTGTTGTTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                               <  2:650970/135‑1 (MQ=255)
                                               cATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACATCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                               <  2:1123815/135‑1 (MQ=255)
                                               cATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                               <  1:584222/135‑1 (MQ=255)
                                                    gCGTTTCGTATGCGCCCACGATCTATCGAAGAATTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACt                                                                          <  1:2292480/135‑1 (MQ=255)
                                                      gTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                         >  2:1715436/1‑118 (MQ=255)
                                                       tttCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGACAACAGCATTTGGTCGGTCCTGTAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTgg                                                                        <  1:1937708/134‑1 (MQ=255)
                                                           gTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTaa                                                                     >  2:2012811/1‑133 (MQ=255)
                                                            tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTaaaaa                                                                  >  1:1198905/1‑134 (MQ=255)
                                                            tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAAc                                                                  >  1:826546/1‑135 (MQ=255)
                                                            tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAAc                                                                  >  2:1812413/1‑135 (MQ=255)
                                                            tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAAc                                                                  >  2:2072222/1‑135 (MQ=255)
                                                            tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAAc                                                                  >  2:694525/1‑135 (MQ=255)
                                                             aTGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACg                                                                                        >  2:1329124/1‑112 (MQ=255)
                                                             aTGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACg                                                                                        <  1:1329124/112‑1 (MQ=255)
                                                             aTGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 >  2:949791/1‑135 (MQ=255)
                                                             aTGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 >  1:2146596/1‑135 (MQ=255)
                                                              tGCGCCCACGAACTATCGAAGAATTTGTTGTTCAACAGCATTTGGTCGTTCCTGGAAAATTTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 <  1:1699777/134‑1 (MQ=255)
                                                              tGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGTAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 <  1:1486608/134‑1 (MQ=255)
                                                              tGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 <  2:2639425/134‑1 (MQ=255)
                                                              tGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 <  2:400140/134‑1 (MQ=255)
                                                               gcgcCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAg                                                               <  1:168138/135‑1 (MQ=255)
                                                                  cccACTAACTATCTAAGAATTTGTTGGTAAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            <  1:357392/135‑1 (MQ=255)
                                                                  cccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            <  2:2030410/135‑1 (MQ=255)
                                                                  cccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            <  2:2568108/135‑1 (MQ=255)
                                                                  cccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            <  2:2848210/135‑1 (MQ=255)
                                                                  cccACCAACTATCCAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCGGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            <  1:2516779/135‑1 (MQ=255)
                                                                  cccAAGAACTATCGAAGAAATTGTTGTTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            <  2:766604/135‑1 (MQ=255)
                                                                   ccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTtata                                                                                          >  1:1120919/1‑104 (MQ=255)
                                                                   ccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 <  1:892573/129‑1 (MQ=255)
                                                                   ccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTa                                                             <  2:2744241/133‑1 (MQ=255)
                                                                   ccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            >  2:854097/1‑134 (MQ=255)
                                                                   ccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            >  2:1585232/1‑134 (MQ=255)
                                                                     aCGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACt                                                                          <  2:1009903/118‑1 (MQ=255)
                                                                     aCGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACt                                                                          >  1:1009903/1‑118 (MQ=255)
                                                                     aCGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGc                                                         >  1:1758084/1‑135 (MQ=255)
                                                                       gAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAg                                                                                                                      <  1:1162582/72‑1 (MQ=255)
                                                                       gAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCa                                                       >  2:2145256/1‑135 (MQ=255)
                                                                       gAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCa                                                       <  1:316096/135‑1 (MQ=255)
                                                                         aCTATCTAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                         <  2:2311171/99‑1 (MQ=255)
                                                                         aCTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAg                                                      >  2:2883602/1‑134 (MQ=255)
                                                                                aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCTTGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTAttt                                              >  2:1741446/1‑135 (MQ=255)
                                                                                aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACt                                                                          >  2:1673712/1‑107 (MQ=255)
                                                                                aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCa                                                       <  2:1407722/126‑1 (MQ=255)
                                                                                aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTAttt                                              >  1:1863053/1‑135 (MQ=255)
                                                                                aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTAttt                                              >  2:2460656/1‑135 (MQ=255)
                                                                                  gaaATTGTTGGTCACCAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                             <  2:1283029/134‑1 (MQ=255)
                                                                                  gaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                             <  1:2158351/134‑1 (MQ=255)
                                                                                  gaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                             <  1:2725637/134‑1 (MQ=255)
                                                                                  gaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                             <  1:1585232/134‑1 (MQ=255)
                                                                                     aTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAg                                           <  2:1552229/133‑1 (MQ=255)
                                                                                     aTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAg                                           <  2:674760/133‑1 (MQ=255)
                                                                                     aTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGc                                         <  1:2895542/135‑1 (MQ=255)
                                                                                     aTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGc                                         <  2:269796/135‑1 (MQ=255)
                                                                                      ttgttgGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCt                                        <  1:2588000/135‑1 (MQ=255)
                                                                                        gttgGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTc                                       >  1:2169395/1‑134 (MQ=255)
                                                                                        gttgGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTc                                       >  1:715482/1‑134 (MQ=255)
                                                                                          tgGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAg                                                                                                                      <  1:2441299/53‑1 (MQ=255)
                                                                                          tgGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAg                                                                                                                      >  2:2441299/1‑53 (MQ=255)
                                                                                              cAACAGCAGTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTaaa                                >  1:285687/1‑135 (MQ=255)
                                                                                                         ggtcggtcCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCg                     >  1:1536926/1‑135 (MQ=255)
                                                                                                                ccTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAAtttt               <  1:2491354/134‑1 (MQ=255)
                                                                                                                ccTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAAtttt               <  1:2269438/134‑1 (MQ=255)
                                                                                                                ccTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAAtttt               <  1:1262133/134‑1 (MQ=255)
                                                                                                                ccTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAAtttt               <  2:1766797/134‑1 (MQ=255)
                                                                                                                 cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                            >  2:2182511/1‑88 (MQ=255)
                                                                                                                 cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg              >  2:150855/1‑134 (MQ=255)
                                                                                                                 cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg              >  1:1508966/1‑134 (MQ=255)
                                                                                                                 cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg              >  1:1616646/1‑134 (MQ=255)
                                                                                                                 cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg              >  1:2375099/1‑134 (MQ=255)
                                                                                                                 cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGa             <  1:1053995/135‑1 (MQ=255)
                                                                                                                  tGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                 >  1:79585/1‑82 (MQ=255)
                                                                                                                    gAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                             <  2:2283783/100‑1 (MQ=255)
                                                                                                                       aaaTTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCt        >  1:1361151/1‑134 (MQ=255)
                                                                                                                       aaaTTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCt        >  2:1221472/1‑134 (MQ=255)
                                                                                                                            aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTa                                                 >  1:1555787/1‑88 (MQ=255)
                                                                                                                            aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc  >  1:425754/1‑135 (MQ=255)
                                                                                                                            aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc  >  2:542415/1‑135 (MQ=255)
                                                                                                                            aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc  >  2:884716/1‑135 (MQ=255)
                                                                                                                            aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc  >  2:937049/1‑135 (MQ=255)
                                                                                                                            aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc  >  1:268590/1‑135 (MQ=255)
                                                                                                                            aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCAGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCTACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc  >  2:2996092/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                
GTTTTTTTATTTACTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACTGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCACC  >  Annotation_U21_Trimmed_contig_4/74996‑75254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑