BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pS4413_qbsFH	65	Δ17 bp	intergenic (–/‑1872)	– / → aadA CDS	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pS4413_qbsFH	64	0	G	.	100.0%	2132.7 / NA	406	intergenic (–/‑1889)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	65	0	A	.	100.0%	2132.7 / NA	406	intergenic (–/‑1888)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	66	0	G	.	100.0%	2132.7 / NA	406	intergenic (–/‑1887)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	67	0	G	.	100.0%	2132.7 / NA	406	intergenic (–/‑1886)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	68	0	A	.	100.0%	2132.7 / NA	406	intergenic (–/‑1885)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	69	0	A	.	100.0%	2132.7 / NA	406	intergenic (–/‑1884)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	70	0	C	.	100.0%	2132.7 / NA	406	intergenic (–/‑1883)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	71	0	T	.	100.0%	2132.7 / NA	406	intergenic (–/‑1882)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	72	0	A	.	100.0%	2132.7 / NA	406	intergenic (–/‑1881)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	73	0	A	.	100.0%	2132.7 / NA	406	intergenic (–/‑1880)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	74	0	A	.	100.0%	2132.7 / NA	406	intergenic (–/‑1879)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	75	0	C	.	100.0%	2132.7 / NA	406	intergenic (–/‑1878)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	76	0	C	.	100.0%	2132.7 / NA	406	intergenic (–/‑1877)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	77	0	C	.	100.0%	2132.7 / NA	406	intergenic (–/‑1876)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	78	0	C	.	100.0%	2132.7 / NA	406	intergenic (–/‑1875)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (154/252); total (154/252)
*	pS4413_qbsFH	79	0	T	.	99.3%	2103.5 / 1.5	406	intergenic (–/‑1874)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base T (0/3); new base . (154/249); total (154/252)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	pS4413_qbsFH	80	0	A	.	99.3%	2101.0 / 4.0	406	intergenic (–/‑1873)	–/aadA CDS	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (154/249); minor base T (0/3); total (154/252)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 406 total aligned reads displayed.

‑‑‑‑‑‑TTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCA  >  pS4413_qbsFH/1‑164

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atgcctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGc                  <  2:18280942/131‑1 (MQ=255)

atgcctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGc                  <  2:2729301/131‑1 (MQ=255)

atgcctttaattaaTTGTTGACAATTAATCATCGGCATAGCATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGc                  <  2:8225415/131‑1 (MQ=255)

  gcctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCac                >  1:11887925/5‑137 (MQ=255)

   cctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacc               >  1:10055616/4‑137 (MQ=255)

   cctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacc               >  1:80346/4‑137 (MQ=255)

   cctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacc               >  2:3413698/4‑137 (MQ=255)

   cctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacc               >  2:4396410/4‑137 (MQ=255)

    ctttaattaaTTGTTGACAATTAATTATCGGCATAGTATATCGGTATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:631796/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:15559432/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              >  1:6307037/3‑137 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:17445655/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  1:8357274/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  1:9627033/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:16831363/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:15836876/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  1:5539607/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              >  1:4525318/3‑137 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:1236311/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:12718904/135‑1 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              >  2:14232694/3‑137 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              >  1:15122180/3‑137 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              >  1:14370129/3‑137 (MQ=255)

    ctttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  2:3710947/135‑1 (MQ=255)

    cgtgaattaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              <  1:59240/133‑1 (MQ=255)

    cgatgattaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCacca              >  2:16659257/6‑137 (MQ=255)

     tttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaa             >  1:16046658/2‑137 (MQ=255)

     tttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaa             >  1:11599633/2‑137 (MQ=255)

     tttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaa             >  1:15851211/2‑137 (MQ=255)

     tttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGAAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaa             <  1:13804473/136‑1 (MQ=255)

      ttgattaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCGCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:13633945/134‑1 (MQ=255)

      ttaattaaTTGTTGACAATTGATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGTGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:842737/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATNGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:18649133/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:7457907/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:8069054/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:7779877/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:7537730/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:15407465/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:16179181/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:11365747/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:16517638/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:7045189/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:6598804/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:9102534/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:78779/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:12377619/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:5913391/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:3692113/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:3250172/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:6032718/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:5500999/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:17972206/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:6128109/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:41760/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:12233184/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:4681189/137‑1 (MQ=255)

      ttaattaaTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGGATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  1:12944860/137‑1 (MQ=255)

      ttaattaaTGGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaa            <  2:4741589/137‑1 (MQ=255)

       taattaatTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCCTACATGCaccaaa            <  2:7195040/137‑1 (MQ=255)

        aattaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacc          >  1:17345438/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  2:2443196/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  2:8851511/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  2:7813166/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         <  2:17176852/137‑1 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         <  1:3207540/137‑1 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         <  1:11415933/137‑1 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         <  1:12748863/137‑1 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  1:15723703/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  1:16433897/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  1:1666631/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         <  1:1768415/137‑1 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  1:1946416/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  2:14060371/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  1:3237174/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  1:3941379/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  1:4748257/1‑137 (MQ=255)

         attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaacca         >  2:10862146/1‑137 (MQ=255)

          ttaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaaccaa        >  1:18562226/1‑137 (MQ=255)

          ttaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCaccaaaccaa        >  2:16037709/1‑137 (MQ=255)

          ttaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTAACAGGAGGAAAAAAATATGTGCCCTTG‑TACCTACATGCaccaaaccaa        >  2:10024255/1‑137 (MQ=255)

           taattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCAccaaaccaaa       <  1:2324745/137‑1 (MQ=255)

            aattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACcaaaccaaac      <  2:148489/137‑1 (MQ=255)

            aattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACcaaaccaaac      <  1:432755/137‑1 (MQ=255)

            aattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACcaaaccaaac      <  1:673356/137‑1 (MQ=255)

            aattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACcaaaccaaac      <  1:703302/137‑1 (MQ=255)

            aatgGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACcaaaccaaac      <  2:10431817/137‑1 (MQ=255)

             attGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCaaaccaaacc     <  2:11968244/137‑1 (MQ=255)

              ttgttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAAccc    <  2:18831195/137‑1 (MQ=255)

              ttgttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAAccc    <  1:15337446/137‑1 (MQ=255)

              ttgttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAAccc    <  2:12177904/137‑1 (MQ=255)

              ttgttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAAccc    <  1:5813743/137‑1 (MQ=255)

              ttgttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAAccc    <  1:5129660/137‑1 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCNCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  >  1:17417135/1‑137 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  >  1:17056037/1‑137 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  >  1:16808094/1‑137 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  <  2:5093156/137‑1 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  >  1:2474401/1‑137 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  >  1:15570125/1‑137 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  >  1:13254706/1‑137 (MQ=255)

                gttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCa  >  2:13372414/1‑137 (MQ=255)

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‑‑‑‑‑‑TTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTG‑CCCCTACATGCACCAAACCAAACCCCA  >  pS4413_qbsFH/1‑164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`