BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	278,744	+A	intergenic (‑239/+75)	PP_0224 CDS (Monooxygenase_ DszC family) ← / ← sctC CDS (sulfur compound ABC transporter ‑ ATP‑binding subunit)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	278,742	1	.	A	95.2%	2250.3 / 104.8	706	intergenic (‑237/+77)	PP_0224 CDS (Monooxygenase_ DszC family)/sctC CDS (sulfur compound ABC transporter ‑ ATP‑binding subunit)	–/–
Reads supporting (aligned to +/- strand): ref base . (4/29); new base A (359/294); total (383/323)
Fisher's exact test for biased strand distribution p-value = 8.85e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 821 total aligned reads displayed.

GCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTC  >  AE015451_phaM1/278606‑278872

  tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGATGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  2:2841150/137‑1 (MQ=255)

  tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  2:15349875/137‑1 (MQ=255)

  tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  1:1485400/137‑1 (MQ=255)

  tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTAGGAACTAATTTTTTGGAATATTTATAGAATGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aa                                                                                                                                  <  2:3021499/137‑1 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGTGGGATTTGTTACATAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  2:2247100/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                >  1:9165070/1‑135 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  1:8120609/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  1:9923712/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  1:13187488/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  1:1846951/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  1:1566289/137‑3 (MQ=255)

    aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaac                                                                                                                                <  2:4451799/137‑3 (MQ=255)

       gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑aaacccg                                                                                                                             >  2:3673854/1‑132 (MQ=255)

        cgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAGTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGc                                                                                                                             >  2:6825674/1‑137 (MQ=255)

         gACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCt                                                                                                                            >  2:7218654/1‑137 (MQ=255)

          aCAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTc                                                                                                                           <  1:10319402/137‑1 (MQ=255)

               cGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTaca                                                                                                                      <  2:15961258/137‑1 (MQ=255)

               cGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTaca                                                                                                                      <  1:2816823/137‑1 (MQ=255)

                gtgtGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacac                                                                                                                     <  2:10687299/137‑1 (MQ=255)

                gtgtGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacac                                                                                                                     <  2:10877539/137‑1 (MQ=255)

                 tgtgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacaca                                                                                                                    <  2:5253202/137‑1 (MQ=255)

                 tgtgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacaca                                                                                                                    <  1:9627296/137‑1 (MQ=255)

                 tgtgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTacaca                                                                                                                    <  1:14591852/137‑1 (MQ=255)

                 tgtgCTGGTCCGGTGGTCCGGTATATATACCCGAATGATGTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGGAAACCCGCTCCTacaca                                                                                                                    <  1:2321184/137‑1 (MQ=255)

                      tggtccggtggtccggTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGc                                                                                                               >  2:4655957/1‑137 (MQ=255)

                      tggtccggtggtccggTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGc                                                                                                               >  2:7717948/1‑137 (MQ=255)

                      tggtccggtggtccggTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATACTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGc                                                                                                               <  1:10001722/137‑1 (MQ=255)

                        gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCt                                                                                                             <  1:1351259/137‑1 (MQ=255)

                        gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCt                                                                                                             <  2:16413621/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTTCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          >  1:1873653/1‑137 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          >  2:5770456/1‑137 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          >  1:466318/1‑137 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  1:4949144/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  1:11722421/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  1:6700272/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  1:15951173/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  1:730816/137‑1 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGATAAACCCGCTCCTACACACATGCCTCaa                                                                                                          >  2:12338700/1‑137 (MQ=255)

                           cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTCTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCACTCCTACACACATGCCTCaa                                                                                                          >  2:6680312/1‑137 (MQ=255)

                             gtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGa                                                                                                        <  2:6129273/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:9788493/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:9303526/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:15621426/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:3257373/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:10880734/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:7852704/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  1:11395230/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGGAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:8740081/137‑1 (MQ=255)

                                gtccggtATATATACCCTAATGATTTATAAAATTCTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                     <  2:12863563/137‑1 (MQ=255)

                                  tcggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTGGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCtgt                                                                                                   <  2:8062815/136‑1 (MQ=255)

                                  ccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCtgt                                                                                                   <  2:17344873/137‑1 (MQ=255)

                                      tatatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCAc                                                                                               >  1:13769567/1‑137 (MQ=255)

                                      tatatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCAc                                                                                               >  2:119247/1‑137 (MQ=255)

                                          tataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa                                                                                           <  1:3800497/137‑1 (MQ=255)

                                              cccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAg                                                                                       >  1:12888898/1‑137 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg                                                                                   >  1:17736791/1‑137 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg                                                                                   >  2:14508795/1‑137 (MQ=255)

                                                  aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACGGTAGGAGCggg                                                                                   >  2:13781641/1‑137 (MQ=255)

                                                   aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGCGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTCCACACATGCCTCAAGATCTGTGCACTGTCGGAGCGGGt                                                                                  >  1:8334616/1‑137 (MQ=255)

                                                     gctttATAAAATTTTTATGAGTTAACTTTTTGGAGTATTTATAGAAGGGGGATTTGTTACGGGGGTGCCGGGCTCTTCG‑C‑GGGTAAACCTGCTCCTACACACATGCCTCACGATCTGTGCACTATAGGAGCGGGttt                                                                                >  2:18229306/3‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGGACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:1941877/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTGAACCCACCCCTCCACACCCGCCCCCAAACCTGTTCCCCGTAGGAGCGGGttt                                                                                >  1:635110/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATTTGTGCACTGTAGGAGCGGGttt                                                                                >  1:12400661/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTTTAGGAGCGGGttt                                                                                >  1:17330601/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:4097413/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:17118404/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  2:8536228/1‑137 (MQ=255)

                                                     gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCACTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGttt                                                                                >  1:365652/1‑137 (MQ=255)

                                                      aTTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAAACCGCCCCTCCACACACGCCTCAAGATCTGTGCACTGTAGGAGCAGGTTTa                                                                               >  1:3047864/1‑137 (MQ=255)

                                                       tttATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTGAACCCACTCCTCCACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGtttcc                                                                              >  1:5925006/1‑135 (MQ=255)

                                                       tttATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCAATGTAGGAGCGGGTTTAc                                                                              >  1:2020103/1‑137 (MQ=255)

                                                         tataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCaa                                                                                                          <  2:1846872/107‑1 (MQ=255)

                                                           taAAATTTTTATGAACTACCTTTTTGGAATATTTAGAGAAGGGGGGTTTGTTACAGAGGTGCCGGGCTCTTTG‑C‑GGGGAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTCACCcgc                                                                          <  2:11301029/137‑1 (MQ=255)

                                                           gaaaaTTTTTATAAACTAACTTTTTGGAATATTTATAGAAGGGGGGTTTGTTACAGGGGTGCCGGGCTTTTCGTG‑GGGA‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCATGGGAGGAGTGGGTTTACCcgc                                                                          <  2:7470076/136‑1 (MQ=255)

                                                            aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGGTCTGTGCAGTGTAGGAGCGGGTTTACCcgcg                                                                         >  2:17741408/1‑137 (MQ=255)

                                                            aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACCGTAGGAGCGGGTTTACCcgcg                                                                         >  2:10237285/1‑137 (MQ=255)

                                                              aaTTTTTGGGAACTAATTTTTTGGAATGTTTAGAGAAGGGGGGTTTGTTACAGGGGTACCGGGCTTTTCG‑G‑GGGGAAACCCGCTCCTACACACATGCCTAAAGATCTGTACACTGTAGGAGTGGGTTTACCCGCGaa                                                                       <  1:1650558/137‑1 (MQ=255)

                                                              aaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCAATGTAGGGGCGGGTTTCCCCACGaa                                                                       >  1:16897293/1‑137 (MQ=255)

                                                                tttttATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGGAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAg                                                                     <  2:3453295/137‑1 (MQ=255)

                                                                   ttATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATTTGTGCAATGTAGGAGCGGGTTTACCCGCGAAAGGGc                                                                  >  2:14823394/1‑137 (MQ=255)

                                                                     aTGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTGACAGGTGTGCCGGGCTCTTCG‑T‑GGGTAAACCCGCTCCTACACACATTCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCg                                                                <  2:8317716/137‑1 (MQ=255)

                                                                                   tGGAATATTTATAGAAGGGGGATTTGTTACAGAGTTGCCGGGCCCTTCG‑CGGGGA‑AACCCGCTCCTACACACATGCCTCAAGATTTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTc                                                  <  2:14210153/137‑1 (MQ=38)

                                                                                          tttAGAAAAGGGGGATTTGTGACAGGGGTACCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCCACACACATTCCCCAATATCTTTGCACTGTAGGAGTGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGcc                                           <  1:14289494/137‑1 (MQ=255)

                                                                                          ttgataGAAGGGGGATTTGTGACAGAGTTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGcc                                           <  2:13121134/134‑1 (MQ=39)

                                                                                            tataAAGGGGGGATTTGTGACAGATGTCCCGGGCTCTTTG‑C‑GGGTAAACCCTCTCCTACACACATCCCTCAAGCCCTGTGCACTGTAGGAGCGGGTTTACCAGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGc                                         <  1:749692/137‑1 (MQ=255)

                                                                                              taGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCACGAAAGGGCCAGAACAGGTTTACTCGGAGGCCGCAg                                       >  1:8820833/1‑137 (MQ=255)

                                                                                                       gATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATTTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGa                              >  2:4941633/1‑137 (MQ=255)

                                                                                                       gATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGa                              >  2:11662575/1‑137 (MQ=255)

                                                                                                       gATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATACCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAAAAGGTTTACTCGAAGGCCGCAGTCCCTAGGa                              >  2:6319696/1‑137 (MQ=39)

                                                                                                            gTTACAGAGGTGCCGGGCTCTTCGTG‑GGGA‑AACCCGCTCCTACACACATGCCTCAAAATCTGTGCACTGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTg                         <  1:18133228/137‑1 (MQ=39)

                                                                                                              tACAGAGGTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAAATCTGTGCACTGTAGGAGCGGGTTTACCCGAGAAAAGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGAAACCTgcg                       >  2:18145544/1‑137 (MQ=38)

                                                                                                                 agagTTGCCGGGCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCaa                    <  2:16976019/137‑1 (MQ=255)

                                                                                                                        ccGGGCTCTTCG‑C‑GGGTCAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGc             >  2:8945628/1‑137 (MQ=255)

                                                                                                                        ccGGGCTCTTCG‑C‑GGGTAAACCCACTCCTCCCCACACGCCTCAAAATCTGTGCACCGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGAACAAGTTTCCTCGGAGGCCGCCATCCCAAGGAACTTGCGCCAGAACCGc             >  1:2669022/1‑137 (MQ=25)

                                                                                                                          gggCTCTTCG‑C‑GGGTAAACCCGCTCCCACACCCATGCCTCAAGATCTTTGTACTGTAGGAGCGGGTTTACCCACGAAAGGGCCGGAACAAGTTTGCCCAGAGGCCGCCGTCCCGAGGAACTTGCGCAAAAACTGCcg           >  1:6919273/1‑137 (MQ=255)

                                                                                                                           ggCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGGTCTGTGCATTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcgc          <  1:15387329/137‑1 (MQ=255)

                                                                                                                           ggCTCTTCG‑C‑GGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCAATGTAGGAGCGGGTTTCCCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAAATTGCGCAAGAACTTCcga          >  2:2560306/1‑136 (MQ=39)

                                                                                                                                ttCG‑C‑GGGTAAACCCGCTCCTACACACATGACTCAAGATCTGTGCAATGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAATAACTGCCGCGTACg     >  2:13577310/1‑137 (MQ=39)

                                                                                                                                ttCG‑C‑GGGTAAACCCACTCCTACACACCTGCCTCAAGATCTGTGCACCGTAGGAGCGGGTTTCCCCGCGAAAGGGCCAGAACAGGTTTCCTCGGAGGCCGCAATCCCGAAGAAATTGCGCAAGAACTGCCGCGTACg     >  1:3321500/1‑137 (MQ=255)

                                                                                                                                  cg‑c‑gGGTAAACCCGCTCCTACACACATGCCTCAAAATCTTTGCACTGTAGGAGCGGGTTTCCCCACGAAAGGGCCGGAACAAGTTTCCTCGGAGGCCGCAATCCCGAGGAAATTGCGCAAAAACCGCCGCATACAct   >  1:4197247/1‑137 (MQ=255)

GCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCG‑C‑GGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTC  >  AE015451_phaM1/278606‑278872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`