BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	4,586,057	+C	intergenic (+140/+75)	PP_4061 CDS (conserved protein of unknown function) → / ← PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,586,056	1	.	C	100.0%	555.4 / NA	144	intergenic (+139/+76)	PP_4061 CDS (conserved protein of unknown function)/PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase)	–/–
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (34/110); total (34/110)

Only 100 of 159 total aligned reads displayed.

GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GG‑TAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACT  >  AE015451_phaM1/4585923‑4586187

gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                   <  2:9516841/137‑2 (MQ=39)

gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                   <  1:11968916/137‑2 (MQ=39)

 aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                  <  2:11191874/137‑3 (MQ=38)

 aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                  <  2:11191830/137‑3 (MQ=38)

 aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                  <  2:18266742/137‑3 (MQ=38)

  ccACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑ccgt                                                                                                                                 <  1:9780684/137‑4 (MQ=38)

   cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑ccgtg                                                                                                                                <  1:15073108/137‑5 (MQ=38)

    aCGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                   >  2:1055520/1‑132 (MQ=38)

    aCGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                   <  1:1055520/133‑2 (MQ=39)

        cACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGc                                                                                                                            <  1:7931696/137‑1 (MQ=38)

        cACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATAGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGc                                                                                                                            <  1:11234153/137‑1 (MQ=38)

             gagTGAACAGCTTATCTGCACTGCTCCATTCAAAGTTTGAGCATTACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑GGATAAACTCGCTCCTACCATGGACCGTGCCGGCCTTGa                                                                                                                       <  2:16707868/135‑1 (MQ=255)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:7762321/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:3334740/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  2:16363246/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:5564218/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:16532864/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  2:5701035/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:5564219/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:1441451/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:13949371/137‑1 (MQ=38)

             gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGa                                                                                                                       <  1:13949370/137‑1 (MQ=38)

                 gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgc                                                                                                                   <  2:15592536/137‑1 (MQ=38)

                 gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgc                                                                                                                   <  2:15190834/137‑1 (MQ=38)

                 gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTG‑GGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:5960883/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGTTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:14101159/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  1:18664035/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:7302073/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:13963264/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  1:4980314/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  1:12136196/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:882713/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:7638709/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:2902312/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  1:2820313/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:15598032/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:4754608/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:5960917/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:4786033/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  1:15602379/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTGGAATgcg                                                                                                                  <  1:8047469/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCGTGAATgcg                                                                                                                  <  2:4007134/137‑1 (MQ=38)

                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑GAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcg                                                                                                                  <  2:3330614/137‑1 (MQ=38)

                   aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATgcgg                                                                                                                 <  1:4309318/137‑1 (MQ=38)

                   aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcgg                                                                                                                 <  1:500475/137‑1 (MQ=38)

                   aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcgg                                                                                                                 <  1:17255788/137‑1 (MQ=38)

                   aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcgg                                                                                                                 <  1:1718424/137‑1 (MQ=38)

                    cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggc                                                                                                                <  1:7431252/137‑1 (MQ=38)

                    cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggc                                                                                                                <  2:8834838/137‑1 (MQ=38)

                    cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggc                                                                                                                <  1:848868/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  2:16715352/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  2:12466837/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  1:11519180/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  1:14991365/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  1:15384960/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  1:15393197/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  2:10581054/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  2:4702355/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  2:17225769/137‑1 (MQ=38)

                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATgcggcg                                                                                                               <  1:717299/137‑1 (MQ=38)

                      gCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGacgc                                                                                                              <  1:13385654/137‑1 (MQ=38)

                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  1:10595647/137‑1 (MQ=38)

                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  1:14276065/137‑1 (MQ=38)

                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  2:11897884/137‑1 (MQ=38)

                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  1:13915300/137‑1 (MQ=38)

                        tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGTCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTg                                                                                                            <  1:18424699/137‑1 (MQ=38)

                        tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTg                                                                                                            <  2:14890810/137‑1 (MQ=38)

                             tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TNAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                       <  1:15888097/137‑1 (MQ=38)

                             tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                       <  2:15822843/137‑1 (MQ=38)

                             tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                       <  1:4880691/137‑1 (MQ=38)

                             tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                       <  1:13326953/137‑1 (MQ=38)

                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  2:5305406/137‑1 (MQ=38)

                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  1:3441629/137‑1 (MQ=38)

                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  2:1866000/137‑1 (MQ=38)

                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCGACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGTTa                                                                                                   <  1:17681712/137‑1 (MQ=38)

                                 ctgcgCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  2:3694492/137‑1 (MQ=38)

                                   gctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAgg                                                                                                 <  2:12917793/137‑1 (MQ=38)

                                   gctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAgg                                                                                                 <  1:16889798/137‑1 (MQ=38)

                                            aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                                                                                                  >  2:4237030/1‑127 (MQ=38)

                                            aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                                                                                                  <  1:4237030/127‑1 (MQ=38)

                                                                             gAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  2:2008840/83‑1 (MQ=21)

                                                                             gAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  2:11951655/83‑1 (MQ=21)

                                                                                                                            tCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATg         >  2:15856991/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAg                                               <  1:2068054/96‑1 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:2967053/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:15581356/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:14762165/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:6657673/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:12511860/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:11605541/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:11605499/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:2406836/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:6350020/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:8130543/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:8452930/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:12439206/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:13708161/1‑137 (MQ=255)

                                                                                                                               tACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCAGCCACACTAATCTCACGCATGCgg      >  2:10899346/1‑137 (MQ=255)

                                                                                                                                   aGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACt  >  2:5509675/1‑137 (MQ=255)

                                                                                                                                   aGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACt  >  2:17660067/1‑137 (MQ=255)

GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GG‑TAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACT  >  AE015451_phaM1/4585923‑4586187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`