Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM12,241,3050TG28.9% 414.3 / 55.3 308intergenic (+52/‑229)gltX CDS (Glutamate‑‑tRNA ligase)/PP_t25 tRNA (tRNA‑Ala)–/–
Reads supporting (aligned to +/- strand):  ref base T (167/49);  new base G (20/68);  total (187/121)
Fisher's exact test for biased strand distribution p-value = 8.48e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 316 total aligned reads displayed.

CCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTG  >  AE015451_phaM1/2241169‑2241441
                                                                                                                                         |                                                                                                                                          
ccTGCGCCAGGCTCTGGACCTACTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGt                                                                                                                                            <  2:5142934/137‑1 (MQ=255)
    cgcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAAt                                                                                                                                        <  1:10583150/137‑1 (MQ=255)
     gcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAAtt                                                                                                                                       <  1:11820968/137‑1 (MQ=255)
      ccAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGGCCTTAAATGCAACGGTAAATTc                                                                                                                                      >  1:9469785/1‑137 (MQ=255)
      ccAGGCTCTGGACCTGCTGGGTGGGATGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGTGAGTGTAATGGTGAAttt                                                                                                                                      >  2:7199898/1‑136 (MQ=255)
       cAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGTCCACGAATGCAATGGTAAATTCa                                                                                                                                     >  1:15442893/1‑137 (MQ=255)
        aGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCCATGGTAAATTcca                                                                                                                                    >  1:7072721/1‑135 (MQ=255)
          gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCACAAAGGCAACGGGAAACTccaca                                                                                                                                  >  1:481143/1‑133 (MQ=255)
          gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAata                                                                                                                                  <  1:17645174/137‑1 (MQ=255)
           ctctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAatat                                                                                                                                 <  2:4001742/137‑1 (MQ=255)
            tctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc                                                                                                                                <  1:10891054/137‑1 (MQ=255)
            tctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc                                                                                                                                <  1:13457789/137‑1 (MQ=255)
             ctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCCACATct                                                                                                                               >  2:16242157/1‑136 (MQ=255)
               ggACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCa                                                                                                                             <  2:4795613/137‑1 (MQ=255)
                 aCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAATCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGTCCGCGAATGTCAGGGTGAATTCCCTATCTCAtt                                                                                                                           >  1:8064279/1‑137 (MQ=255)
                 aCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGGATGCAATGGTGAACTCCATATCGCagt                                                                                                                           >  1:11503061/1‑135 (MQ=255)
                       tgggtgggGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAACGCAATGGTAAATTCAACACCGCACTCGccag                                                                                                                     >  2:2634255/1‑135 (MQ=255)
                        tggtgtgatgTCGAAGAAAGGAAATAAGGAGCGGGAAAAGCTG‑TTGACTTCCATCGCCTGATTCAGCTTCGCACCCAACCAGTGGAAGCAGACGTGCCCGCGAATACAATGATAAACCCAATATCACACTCACCAGc                                                                                                                    <  2:16083619/136‑1 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGGGTGGGAAAAGCTG‑TTGGTTTCCATCGCCTGATTCAGTTTTACACCTAACCGGTGGGAGCGGGCGTGCCCGCGAAGGAAGTGGGAAGTTCAATATCGCGTTCGCTGGc                                                                                                                    <  1:4287385/137‑1 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGTGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGc                                                                                                                    <  2:11528857/137‑1 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCCATGGTAAATTCAACATCGCACTCACCGGc                                                                                                                    >  2:1735103/1‑137 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCCATATCGCATTCACCGGc                                                                                                                    >  2:8135019/1‑137 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAATGTAACGGTAAAGTCAACATCGCATTCGCCAGc                                                                                                                    >  2:7634469/1‑137 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAAGGCAACGGTGAAGTCAACACCTCATTCGCCGGc                                                                                                                    >  1:12471851/1‑137 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGAGTGCCCACGAATGCAATGGTAAATTCAATATCGCATTCGCCgga                                                                                                                    >  1:17889233/1‑136 (MQ=255)
                        gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGACTGATTCAGCTTTGCACCTACCCAGTGGGAGCGGGCGTGCCCGCGTAGTAAACGGTAAATTCAATATCGCCTTCGCCggt                                                                                                                    >  2:3145032/1‑136 (MQ=255)
                        gggtggggtgTAGAAGAAAGAAAAAAAAGAGTGGGAAAAGTTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGAGTGCCCGCTAATGCAATGGTAAATTCCAGACAGCATTTGCCGGc                                                                                                                    >  2:1965019/1‑137 (MQ=255)
                         ggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCa                                                                                                                   >  2:15609861/1‑137 (MQ=255)
                             gggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGcc                                                                                                               >  2:12401144/1‑137 (MQ=255)
                                tgtCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAACGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGc                                                                                                            >  2:17062313/1‑137 (MQ=255)
                                 gtCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAATGCAAGGGTAAATTCCACATCGCCTTCGCCGGCACGCCCGCt                                                                                                           >  2:12262290/1‑137 (MQ=255)
                                   cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTTCCCGCGAATGCAACGGTAAATTCAATCTCGCACTCGCCGGCACGCCCACTcc                                                                                                         >  2:12084664/1‑137 (MQ=255)
                                   cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCTCGAATGCAACGGGAAAATCCATATCTCATTTGCCGGCACACCCACTcc                                                                                                         >  1:3638363/1‑137 (MQ=255)
                                   cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAACTCAATATCGCATTCGCCGGCCCGCCCGCTcc                                                                                                         >  2:13803410/1‑137 (MQ=255)
                                   cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGGAAATTCCACATCACACTCTCCGGCACACCCACTcc                                                                                                         >  1:3911074/1‑137 (MQ=255)
                                      agaaagaaAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGAAAGGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCcaa                                                                                                      >  1:4077324/1‑136 (MQ=255)
                                      agaaagaaAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAATGAAACGGTAAATTCAATATCGCATTCGCCAGCACGCCCACTCCcac                                                                                                      >  2:669593/1‑137 (MQ=255)
                                      agaaagaaAAAAAAGATTGGGAAAAGTTT‑TTGGCTTCCATTGCTTGATTCGGTTTTGCACCGAACCGGTGGGAGCGGGTGGGCCCGAGAATGAAGGGGGAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCcac                                                                                                      <  2:16461334/137‑1 (MQ=255)
                                       aaaagaaaACAAAGGGTGGGAAAAGCTG‑TTGGTTTCCATCGCCTGATTCGGCTTTGCACCTAACCGGTGGGAGCGGGCGTTCCCGCGAATGCAATGGTAAATGCAATATCGCATTCGCCGGCACGCCCGCTCCcaca                                                                                                     <  2:12168188/136‑1 (MQ=255)
                                        aaagaaaACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCGCGAATGCAATGGTGAATTTGATGTCGCATTTGACGGGACTCCCGCTCCcaagg                                                                                                    >  2:7600519/1‑134 (MQ=255)
                                           gaaaACAAAGAGCGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTTCCCGCTGAGGTCAAGGTCAATTCAACACCGCATTCGCCGGCCCGCCCGCTCCCCCAGGcc                                                                                                 >  2:10464301/1‑137 (MQ=255)
                                            aaaaCAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAACGCAATGGTAAATTCAACATCGCATTCGCCGGCACGCCCGCTCCCCCAGGAC‑a                                                                                               >  2:2817855/1‑137 (MQ=255)
                                            aaaaC‑AAGGGTGGGGAAAGTTTTTTGGCTTCCGTCGCCTGATTCGGTTTTGCACCGAACCGGTGGGGGTGGGCGTGCCCGCGAGTGAAGTGGGAAATTCACTCTCGCGTTCGCCGGCACGCCCGCTCCCACAGGCC‑a                                                                                               <  2:14410290/137‑1 (MQ=255)
                                               aCAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCCATATCGCCTTCGCCGGCACGCCCGCTCCCACAGGCC‑AACt                                                                                            >  2:13641061/1‑137 (MQ=255)
                                                 aaaGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGTGAACGGCAGGGTGAAACCAACATCCCATTCGCCCGCCCACCCCCTCCCACAAACC‑aaacca                                                                                          >  1:6363954/1‑133 (MQ=255)
                                                         ggAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCCCGAACGCAACGGTCAACTCCACCCCACACTCCCCGGCACACCCCCTCCCACAAGCC‑AACC‑CAACCCCaaa                                                                                 >  2:5069981/1‑137 (MQ=255)
                                                          gAAAATCTT‑TGGGTTTCCGTCGTTTGATTCGTTTTTACACCTAACCAGTGGGGGTGGGCGTGCCCGTGGGTGCAATGGGAAGTTAAATACCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCaaaa                                                                                <  2:2210346/137‑1 (MQ=255)
                                                          gAAAAGTTG‑TTGGTTTCCGTCGCTGGGTTAAGTTTTACACCGAACAGGTGGGGGGGGGTGTTCCAGGGAGTGAAGGGGGAAGTTCAATACCGCCTTCACAGGAACCCCCGCTCCCACAGGCC‑AACT‑AAGCCCCaaaa                                                                                <  2:6894917/137‑1 (MQ=255)
                                                          gAAAAGCTG‑TTGGCTTCCATCGCTTGGTTAAGCTTTGCACCTAACCAGTGGGAGTGGGCGTGCCCGCGAATGCAGTGGGAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCaaaa                                                                                <  2:17677575/137‑1 (MQ=255)
                                                                ttg‑tgGGCTTCCGTTGCTGGATTCGGTTTGGAACCGAACCGGTGGGAGGGGGTGTGCCCGGGAGTAAAATGGGAAGTTCAAGCTCTCGTTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCgg                                                                          <  2:6102079/136‑1 (MQ=255)
                                                                ttg‑tgGGCTTCCATCGCTTGGTTCGGTTTTGCACCTAACCGGGGGGAGGGGGTGTGCCTGAGAAGGAAATGGGAAATTCAATATCGCCTTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCgg                                                                          <  2:14628907/136‑1 (MQ=255)
                                                                ttG‑TTGGTTTCCGTTTCCTGGTTCAGTTTTGCACCTAACCGGTGGGAGCGGGTGTGCCTGAGAGTGCAATGGGAAGTTCACTCTCGCATTCGCTGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCgg                                                                          <  2:14826493/136‑1 (MQ=255)
                                                                cTG‑TTGGCTTCCATCGCCTGATTCAGTTTTACACCTAACCGGGGGGGTCGGGCGTGCCTGCGAATGAAATGGGAAATTCAATATCACATTAGCCGGCACGCCCACTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCgg                                                                          <  2:8062622/137‑1 (MQ=255)
                                                                 ctcttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCATTGGGAGCGGGCGTGCCCGCGAAGGCAGTGGGAAGTTCAATATTGCATTAGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCgg                                                                          <  2:12971591/134‑1 (MQ=255)
                                                                   tttGGTTTCCATCGCAGGATTGAGTTTTACACCGAACCGGGGGGGGGGGGCGTGCCCAGAAGGGCAAGGGGAAACCCAATCTCGCATTCGCCGGCACACCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCgggg                                                                        <  2:9469785/136‑1 (MQ=255)
                                                                     tGGTTTCCGTCGCTGGATGCAGTTGTGCACCGAACCGGGGGGGGGGGGTGTGCCCGTGAGTGAAATGGGAAGTTAAATATCCCGTTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTa                                                                      <  1:8649729/137‑1 (MQ=255)
                                                                       tcTTCCCTCGCCGGCTTCATTTTTACCCCCACCCATGGGGAGCGGGTGTCCCTGAGAATAAAGGGGGAAGTCCACTGTCCCCTTCCCCGACACCCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAgg                                                                    <  2:7876918/136‑1 (MQ=255)
                                                                       tcTTCCATCGCTGGATTCATTTTTACACCGAACCAGTGGGAGCGGGCGTACCCGCGAATAAAATGGGAAATCCACTCTCGCCTTCGCCGACACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAgg                                                                    <  2:13147433/136‑1 (MQ=255)
                                                                       gCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGTAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACCGGAC‑AACT‑AAGTCCCAAAAAACCGGGGtcgg                                                                    >  2:4561627/1‑134 (MQ=255)
                                                                       gCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑GAGCCCCAAAAAAACGGGGTAgg                                                                    >  2:1503606/1‑137 (MQ=255)
                                                                       gCTTCCATCGCCGGGTTAATTTTGGAACCGAACCGGTGGGAGTGGGCGTGCCCGCGAATACAATGGGAAATCCAATACCGCCTTCGCCGACACACCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAgg                                                                    <  2:4260997/137‑1 (MQ=255)
                                                                         ttCCATCGCTTGATTCATCTTTACACCTAACCGGTGGGAGCGGTCTTCCCCGCGAGTCCAATGGGAACTCCACTCCCCCATTCTCCGCCACCCCCGCCCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGc                                                                  <  1:18846135/137‑1 (MQ=255)
                                                                          tCCATCGCCTGGTTCAGCTTTGCCCCTAACCAGTGGGAGCGGGCTTGCCTGCGAATTAAAGGGTAAATTCACTATCGCATTCCCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCg                                                                 <  2:5699457/137‑1 (MQ=255)
                                                                            cATCGCTGGATTCGGTTTGGCACTGAACCGGTGGGGGTGGGTGTGCCTGCGAGTGAAGGGGGAAGTTCAATATCGCCTTCGCTGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGt                                                               <  2:6626174/137‑1 (MQ=255)
                                                                            cATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCCATATCGCATTCGCCGGCACACCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGGGGt                                                               >  2:9159493/1‑137 (MQ=255)
                                                                            cATCGCCCGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAGTGCAATGGGAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGt                                                               <  2:10889694/137‑1 (MQ=255)
                                                                            cATAGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGt                                                               <  2:8609281/137‑1 (MQ=255)
                                                                                 ccTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGTCCTCGAATGCAATGGTAAATTCAATATCGCATTCGCCAGCACGCCCCCTCCCCCAGGCC‑AACC‑AAGCCCCAAAAAAACGGGGTAGGGAGGTGAGTg                                                          >  2:15996163/1‑137 (MQ=255)
                                                                                    gATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCACGAAAGAAAAGGTAAAGTCCACATCACACTCGCCAGCACACCCCCTCCCACAGGAC‑AACC‑CAACCCCAAAAAAACAGGGTGGGGAGGTGAGTGAtt                                                       >  1:16063090/1‑137 (MQ=255)
                                                                                        cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTCAATTCCATATCGCCTTCGACAGCCCGCCCGCTCCCACAGGAC‑AACC‑CAGCACCAAAAAAAAGGGGTGGGGGGGTAAATGGTttttt                                                   >  2:6224300/1‑136 (MQ=255)
                                                                                        cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAACGAAATGGTGAAGTCCACATCGACCTCACCGGCACGCCCGCTCCCCCCAGCC‑ACCT‑CAACCCCAAAAAAACAGGGTAGGGGGGTAAGTGAGTGttc                                                   >  1:3374186/1‑136 (MQ=255)
                                                                                        cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAATGCAAGGGTAAATTCAATATCGCATTCGCCGGCACACCCGCTCCCACAAGAC‑AACC‑AAGACCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGttg                                                   >  2:7418942/1‑136 (MQ=255)
                                                                                              tGCACCTAACCAGTGGGAGCGGGCGTGTCCTCGAAGGAAATGGTAAAGTCAATATCACACTCGCCAGCAAGCCCGACCCCCCAAGCC‑AACC‑AAGCCCCAAAAAAACGGGGTAGGGCGGTCAATGATTTTTATCtgtg                                             >  2:11788218/1‑137 (MQ=255)
                                                                                              tGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGTAAACTCAATATCGCATTCTCCAGCACGCCCGCCCCCACAAGCC‑AACT‑CAGCCCCAAAAAAACGGGGTAGGGGGGTGAGTGATTGTTATCtgtg                                             >  2:6808687/1‑137 (MQ=255)
                                                                                                 aCCTAACCAGTGGGAGCGGGAGTGCCCACGAATGTAACGGTAAATTCCATATCGCACTCGCCGGCACGCCCACTCCCACAAGAC‑AACC‑AAGCCCCAAAAAAACAGGATAGGGAGGTAAGTGAGTGTTATCTGTGaaa                                          >  2:18565936/1‑137 (MQ=255)
                                                                                                  ccTAACCAGTGGGAGCGGGGGTGCCCGCGAACGCAAGGGTGAACTCCACCTCGCCCTCCCCCGCACACCCACTCCCACAGGCCAAACC‑AACCCCCAAAAAACAGGGG‑AGGGGGGGAAGTGATTGTTCTCTGTGaaaa                                         >  2:10172099/1‑137 (MQ=255)
                                                                                                  ccTAACCAGTGGGAGCGGGCGTGCCCACGAATGCAATGGTGAAGTCAATATCGCATTCGCCGGCAAACCCGCTCCCACAGGCA‑AAAT‑CAGACCCAAAAAACCGGGGTAGGGAGGTAAGTGATTGTTGTCTGTGaaaa                                         >  2:13409123/1‑137 (MQ=255)
                                                                                                   cTAACCAGTGGGAGCGGGTGTGTCCGCGAAGGCAAGGGTCAATTCCATCCCCCACTTGCCAGTACGCCCGCCCCCACAAGCC‑AAAC‑AAGCCCCCAAAAAACAGGGTGGGGGGGGAAGTGAGTGTTTTCTGTTaaaaa                                        >  1:2251373/1‑137 (MQ=255)
                                                                                                   cTAACCAGTGGGAGCGGGCGTGCCCACGAACGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGGGGTAAGTGATTGTTATCTGTGaaaaa                                        >  2:18074475/1‑137 (MQ=255)
                                                                                                   cTAACCAGTGGGAGCGGGCGTGCCCACGAACGCAAGGGGGAACTCAATACCGCATTCTCCAGCACACCCACCCCCCCAAGCC‑AAATAAAACCCCAAAAAAAAGGGG‑GGGGGGGTGAGGGGGTGTTCTCTGTGgaaaa                                        >  2:343306/1‑137 (MQ=255)
                                                                                                   cTAACCAGTGGGAGCGGGCATGCCCGCGAATGGAATGGTAAATTCAATACCGCATTCGCCGGACCACCCGCTCCCACAGGCC‑AACC‑AAGACCACAAAAACCGGGGTGGGGAGGTGAGTGATTGTTGTCTGTGaaaaa                                        >  2:11989778/1‑137 (MQ=255)
                                                                                                    tAACCATTGGGAACGGGCGTGCCCGCGAGTGCAATTGGAAATCCAGTATCTCATTCCCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAGACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGaaaaaa                                       <  2:13916154/137‑1 (MQ=255)
                                                                                                       ccAGTGGGAGCGGGGGTGCCCGCGAATGCCATGGTAAATTCCATATCGCATTCGCCGGCACGTCCGCTCCCACAGGCC‑AACT‑ACGCACCAAAAAACAGGGGTAGGGGGGTAAGTGGTTGTTATCTGTGAAAAAAttt                                    >  2:12672961/1‑137 (MQ=255)
                                                                                                        cAGTGGGAGCGGGCGTGCCCGCGAACGACAGGGTAAACTCAACATCACACTCGCCGGCACGCCCACTCCCACAGGAC‑AAAT‑AAGACCCAAAAAACAAGGGTAGGGCGGTAAGTGAGTGTTATTTGTGAAAAAActtt                                   >  2:5334220/1‑137 (MQ=255)
                                                                                                              gAGCGGGGGTGCCCGCGAAGGGAAGGGTAAACTCAACAGCGCATTCGCCAGCACGGCCGATCCCAAAGGAA‑AAAC‑AAGCCCCAAAAAAACGGGGTGAGGCGGTAAGTGATTGTTGTCTGTGAAAAAATTTTTGaaca                             >  2:1848187/1‑135 (MQ=255)
                                                                                                                  gggAGTGCCCGCAAAAGCAATGGTAAACTCAACATCACATTCGCCGGCACACCCACTCCCCCAAGCC‑AAAC‑CAACCCCAAAAAAACAGGGTGGGGGGGTAAGTGGTTGTTATCTGTGAAAAAACTTTTTGATActtt                         >  2:11032807/1‑137 (MQ=255)
                                                                                                                   ggCGTGCCCGCGAAGGCAATGGTAAATTCAACATCGCATTCGCCGGCACACCCGCTCCCACAGGCC‑AAAT‑AAGACCCAAAAAACCAGGGGCGGGCGGTAAGTGGGTGGTCTCTGTGAAAAAATTTTTGAATAttttt                        >  2:1835722/1‑137 (MQ=255)
                                                                                                                    gCGTGCCCGAGAGTATAAGGGGAAATTCAATATCTCATTCACCGGAACTCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTg                       <  2:10004518/137‑1 (MQ=255)
                                                                                                                     cGTGCCCGCGAAGGCAAGGGTAAACTCCATATCGCCTTCTCCGGCACGCCCACTCCCACAAGAC‑AAAC‑AAGCCCCCAAAAACCAAGGGAGGGAGGTAAGTGGTTGTTGTCTGTGAAAAAATTTTTGAACACTTTTGa                      >  2:9089183/1‑137 (MQ=255)
                                                                                                                      gTGCCCGAGAATACAATGGGAACTTCAACCTCGCCTTCACCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGaa                     <  2:10685690/137‑1 (MQ=255)
                                                                                                                              gAATGCAATGGTAAATCCAAGATCGCCTTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGtt             <  2:4448569/137‑1 (MQ=255)
                                                                                                                              gAAGGCAAGGGGAAGTCCAATATCCCATTCACCGGCACACCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGtt             <  2:2867444/137‑1 (MQ=255)
                                                                                                                                gtGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTg           <  2:8810943/136‑1 (MQ=255)
                                                                                                                                aTGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTg           >  2:2713316/1‑137 (MQ=255)
                                                                                                                                 tGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa          >  2:16672607/1‑137 (MQ=255)
                                                                                                                                 tGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa          >  2:17258329/1‑137 (MQ=255)
                                                                                                                                 tGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa          >  2:17803240/1‑137 (MQ=255)
                                                                                                                                 ggaagtGGGAAATTCAATATCGCATTCGCTGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa          <  1:14720298/132‑1 (MQ=255)
                                                                                                                                    aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAg       >  1:12001741/1‑137 (MQ=255)
                                                                                                                                         tAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTg  >  1:17831282/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTG‑TTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACT‑AAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTG  >  AE015451_phaM1/2241169‑2241441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑