BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,344,308	0	G	A	35.8%	101.0 / 31.6	123	intergenic (+39/‑117)	PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base G (45/25); new base A (39/0); total (98/25)
Fisher's exact test for biased strand distribution p-value = 2.62e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 159 total aligned reads displayed.

GGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344174‑4344443

ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGTGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGCCAGACCAAGCaccacc                                                                                                                                        >  2:11092257/1‑137 (MQ=255)

ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCGACCTAGCaccacc                                                                                                                                        >  1:8907934/1‑137 (MQ=255)

ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCAATCGAACaccacc                                                                                                                                        >  2:7850231/1‑137 (MQ=255)

ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATCGCACAccgcc                                                                                                                                        >  2:4196064/1‑137 (MQ=255)

ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGACCGAGCaccacc                                                                                                                                        >  2:5534543/1‑137 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGTAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:12798782/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGTATTTATACCGAATTGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:17684329/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:9617630/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:6187655/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:6094811/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:15458180/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:2579953/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:2346876/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:16472797/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:17747715/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:2457815/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:8400712/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:6967808/137‑1 (MQ=255)

 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:16762935/137‑1 (MQ=255)

  cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                      <  1:7388429/137‑1 (MQ=255)

  cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                      <  2:1080602/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  2:4956863/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  2:9243030/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  1:5830527/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  2:15012269/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  1:13827130/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  1:1853623/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  1:14775573/137‑1 (MQ=255)

      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  2:2162206/137‑1 (MQ=255)

        cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                                <  2:465236/137‑1 (MQ=255)

          tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                              <  2:16148116/137‑1 (MQ=255)

          tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                              <  1:10613764/137‑1 (MQ=255)

            aaGGTGCGTTGTGCAGTGGCAGACGCTTGCATTTATACCAAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTGGTACGTTGCAGCGCCTGCCAGATTGAGCACCGCCCGCGCGGCacac                                                                                                                            <  1:13282299/137‑1 (MQ=255)

            aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                            <  2:9272282/137‑1 (MQ=255)

            aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                            <  1:13282342/137‑1 (MQ=255)

            aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                            <  2:10410824/137‑1 (MQ=255)

            aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                            <  1:12047586/137‑1 (MQ=255)

             aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAGATCGAGCACCGCCCGCGCGGCacaca                                                                                                                           >  2:18497494/1‑136 (MQ=255)

             aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAAATTGAGCACCACCCTCGCGGCACACg                                                                                                                           >  2:11372620/1‑137 (MQ=255)

              ggTGCGTTGGGCAGTGGCAGACGCTTGCATCTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCCAGCACCACCCCCACAGCACACGa                                                                                                                          >  2:8920123/1‑137 (MQ=255)

               gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGCGCACCACCCAAACGGCACACGac                                                                                                                         >  2:12471970/1‑136 (MQ=255)

                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAGATCGAGCACCACCCCCACGGAACACCATCTTGCa                                                                                                                   >  1:12229443/1‑137 (MQ=255)

                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAGATCGAGAACCCCCCCCCCAGAACACAAACTGGCa                                                                                                                   >  1:4793488/1‑137 (MQ=255)

                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATCGAGCACCACCCGCGCGGCACAAGATCTGGCa                                                                                                                   >  2:2860739/1‑137 (MQ=255)

                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAACAACCACGCGGAACAAGATCTGGCa                                                                                                                   >  1:2103621/1‑137 (MQ=255)

                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCTCCAAATCCAGCACCACCCACACGGCACAAGATCTGGCa                                                                                                                   >  2:16389959/1‑137 (MQ=255)

                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCCGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAAAACCACCCACGCGGAACACAACCCGGCa                                                                                                                   >  2:3343373/1‑137 (MQ=255)

                          aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGCCAAATCGAACACCGCCCACACGGCACACAAGCTGGCAGgcgc                                                                                                              >  2:8583863/1‑137 (MQ=255)

                            tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCGAGCACCGCCCGCGCAGCACACGATCTGGAAAGAGCTg                                                                                                            >  2:1371700/1‑137 (MQ=255)

                             ggCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGATGCCCGAGCGAGCACCACCCACACGGCACAAAACCTGGCAGGCGCTG‑c                                                                                                          >  1:12080752/1‑137 (MQ=255)

                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCGAGCGAGCACCACCCCCGCGGCACACGAGCCGGCAGGAGCTgtaa                                                                                                         >  1:6675562/1‑134 (MQ=255)

                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCTCGATCGAGCACCGCCCGCGAGGCACACGATCTGGCAGGCGGTG‑cga                                                                                                        >  2:16048446/1‑135 (MQ=255)

                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCTCGCACCACCCACACCGCCCACAACCTGGAAAGCGACG‑CAc                                                                                                        >  1:5598062/1‑137 (MQ=255)

                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCAAGCACCACCCGCAAGGCACACAAGCTGGCAAGCGCTG‑CAc                                                                                                        >  2:10028361/1‑137 (MQ=255)

                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGACCGAACACCACCCAAACGGCAAACAATCTCGCCAGCGCTG‑CAc                                                                                                        >  2:12460380/1‑137 (MQ=255)

                                aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGTGGCACACGATCTGGACGGCGATG‑AAcc                                                                                                       >  2:4166103/1‑136 (MQ=255)

                                aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCACCCACGTAGCACAAGATCTCGCCGGCGCTG‑AACt                                                                                                       >  2:12233566/1‑137 (MQ=255)

                                aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCCAATCGAGCACCGCCCACGCGGCACACGACCTGGCAGGCGCTG‑CACt                                                                                                       >  2:12233600/1‑137 (MQ=255)

                                    gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTTCCAGATCAAACACCGCCCGCTCTGCACACAATCTGGCAGGTGCTT‑CCCTACTg                                                                                                   >  1:7571838/1‑137 (MQ=255)

                                    gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGTCCGAGCGAGCACCACCCCCGCGGAACACAATCTGGCAGGGGATG‑CAATACTg                                                                                                   >  1:6568811/1‑137 (MQ=255)

                                         cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGTCAGGTCGAGGGCCGCCCGCGCGGTACACGATCTTGCAGGTGGTG‑TCCTCTTGGGGGc                                                                                              >  2:6572750/1‑137 (MQ=255)

                                         cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCGATCGAGCACCGCCCGCGCGGCACACGATCTGGGCGGAGCTG‑CCCTATTTGAGGc                                                                                              >  2:14263156/1‑137 (MQ=255)

                                           tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGTGCATTAACGATATTGTACGTTGCAGCGTCTGTCAGATCGAGCAACACCCACGCAGCACACAAGCTTGCAGGCACTG‑CACTATTTGAGGCgg                                                                                            >  2:14832430/1‑136 (MQ=255)

                                           tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTACCAGATCGAGCACCGACCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGt                                                                                            >  1:17955382/1‑137 (MQ=255)

                                           tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCTCCCAATCCAACACCGCCCGTACGGCACACGATCTTGCAGGCGCTG‑CACTATTTGAggagc                                                                                            >  2:7335498/1‑134 (MQ=255)

                                                 ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTTTCCGGGCGAGCACCGCCCNCGCGGAACACAAGCTTGCAAGGGCTT‑TACTCTTTGGGGGGTACGccc                                                                                      >  1:15245362/1‑137 (MQ=255)

                                                  cGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAAAACCGACCGCGCGGCACAAGATCTGGCAGGGGCTG‑CAATATTGGAGGCATACGcccc                                                                                     >  2:11446671/1‑137 (MQ=255)

                                                   gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCGCCAAAGCAAAAACCACCCACACAGCACACGACCTTGCAGGAGATG‑CACCCTTTGGGGAGTACAccccc                                                                                    >  1:13190603/1‑136 (MQ=255)

                                                     aTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCACGTGGCACCCGATCTTGCCGGTGCTG‑CACTATTTGCGGAGTACGCCCCGTc                                                                                  >  2:12800576/1‑137 (MQ=255)

                                                      tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAGAGCGAGAACCACCCACGCGGCACAAAATCTGGAAAGAGCTG‑CACTATTTGAGGAGTACACCCCGTcc                                                                                 >  1:15831146/1‑136 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTTTCCGATTTAGCACCCCCCCCGCTGCCCCCAATCCTGCCAGCGCTG‑TACTCTTTTAGGCGTCCACCCCGTccc                                                                                >  2:15653472/1‑135 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCCAATCGAGGACAACACACGCGGAACACAAGGTGGGAGGTGCGG‑GAATAGTGGGGGGGTGCGCACCGGcac                                                                                >  2:5622965/1‑137 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCTTCAGGACGAACACCCCCCACGCGGCACACAAACTGGCAGGAACTGCCACCTTTTG‑GGCGTACCCCCCGTccc                                                                                >  2:5264365/1‑135 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCGTCCGATCGAGCACCACCCCCACGGCACAAAAGCTGGCAGGAGCTG‑TACTCTTGGGGGCGTACGCCCCATcac                                                                                >  2:15629544/1‑137 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCGCCAGACCAAACACCGCCCCCGCGGCAAACAAGCTTGCCGGAGATG‑CACTCTTTGAGGCGTACGCCCCGTcaa                                                                                >  2:3524877/1‑136 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTTTCCGATCTCGCCCCCCCCCCGCCGCACACGACCTTGCCGGCGCTG‑CACCCTTTGAGGCGTACACCCCCTccc                                                                                >  2:17664556/1‑135 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGTCCGACCTAACACCACCCACACGGCACACAACCTTGCCAGCGCTG‑CACTCCTTGAGGCGTCCCCCCCATccc                                                                                >  2:7053648/1‑135 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGTCCAAGCTAGCACCCCCCCCACAGCACCCAAGCTGGCAAGCGATT‑TACCCTTTGAGGCGTACGCCCCCTccc                                                                                >  1:17501529/1‑135 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAGAGCGAGAACCCCCCACCTAGCAAACAACCCTGCAAGAGCTG‑CACTCTTGGAGGCGGACGCCCCATcac                                                                                >  1:10079404/1‑137 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCGCCCGATCGAGCACCGCCCCCGCGGCACACAACCTTGTAGGCGCTT‑CACCATTTGAGGAGTACGCCCCTTcac                                                                                >  2:3411010/1‑137 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCGCCAAAGCGAAAACCCCCCCCCCGGCACCCAACCCTGCAAGCGCTT‑CCCTCCTTGAGGAGTACACCCCCTccc                                                                                >  1:4151860/1‑135 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCCGAACACGGCCCGGCCGGCGCTG‑TCATATTTGAGGCCTACACCCCATcca                                                                                >  2:4678960/1‑135 (MQ=255)

                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGCCCGATCGAGCACCGCCCGCGCGGAAAAAAATCTTGCAGGCGCTG‑CACCCTTGGAGGCGTACACCCCGTcac                                                                                >  2:8998455/1‑137 (MQ=255)

                                                         gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCGTTCCAAATCTAGACCCACCCACGCGGCACAAAAACTGGCAGGCGCTG‑GCCCAATGGAGGCGTAAACCCCATccccc                                                                              >  2:16499967/1‑133 (MQ=255)

                                                                      tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAGATCTAGCACCGCCCACGCGGCACACGATCTGGCAGGCGCTG‑ACCTCTTGGAGGCGTACACCCCGGCACATTACCATCccacca                                                                 >  2:8150211/1‑137 (MQ=255)

                                                                      tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCAAACACCACCCACACAGCAAACAATCTGGCAGGCGCTG‑CACCACTGTAGGCGTACGCCCCGTCCCACTATCCTCCCGCCa                                                                 >  2:1755397/1‑137 (MQ=255)

                                                                           aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCGATCCAACACCCCCCGCGCAGCACCCAATCTGGCAAGCACTT‑TACCACTTGAAGCGTACACCCCATAAAAGTATCACCACGCCACTGTc                                                            >  2:7707491/1‑137 (MQ=255)

                                                                                ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGACCGAACACCGCCCGCGCGGCACACGATCTGGCAGGAGCCG‑CACCACTAGAGGCGTACGCCCCGTCACCTTATCATCCCGCCACTGTCGtcac                                                       >  2:13932717/1‑134 (MQ=255)

                                                                                     gggCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAGATCGAGCACCGCCCGCGCGGAACACAATCTGGCAGGAGATG‑CAACATTGGAGGCGTACGCCCCGTCACATTATCATCCCACCACTTTCGTAACCCGcc                                                  >  2:17238295/1‑137 (MQ=255)

                                                                                                                aGCGTCCGCCAAACCGAGCACCGCCCGCGCGGCACACAATCTGGCAAACGCTG‑CCCCACTTGAGGCGTCCGCCCCGTCCAAGTCTCATCACACCACCGTCGTGATCTGTCGGCCAAATCTACGCCACCAGTTACCCg                       >  2:17264260/1‑137 (MQ=255)

                                                                                                                aGCGGCTGCCCAATCGCGCACCGCCCACGCGGCACACAACCTGGCAGAAGACG‑CACCATTGGAGGCGTACGCCCCATCCCATTATCATCAAGCCACCGGCGTAATCTTCCGGGAAACTCTCTGCCACAGGTCACACg                       >  2:13149265/1‑137 (MQ=255)

                                                                                                                aGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGAGCTGGCAAGCGCCT‑CACCATTTGAGGCGTACGCCCCATCCAATTCTCCTCCCACCCCTGTCTTCACCTTTCCGCCCAATCTATGTCCCAAGTCAaccc                       >  2:3211353/1‑136 (MQ=255)

                                                                                                                  cggcTGCCCGATCGAGCACCACCCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCCCACTATCCCCACGCCCCTGTCGTAATCTTCCCGCCAACTCTCTGCCCAAAGTCACCCGGc                     >  2:9231121/4‑137 (MQ=255)

                                                                                                                  cgcccgccAAATCGAGCACCGCCCACGCGGAACACGATCTGGCAGGCGCTG‑CACCATTGGAGGCGTCCGCCCCATCAAATTATCATCAAACCACCGTCGTAACCTGCCGGACAAACCTATGCCAAAAGTCAACCgaa                     >  2:13157264/1‑135 (MQ=255)

                                                                                                                  cgcccgccAAACCGAACACCACCCACGCGGTACACGACCCAGCAAGCGCTA‑TACCATTGGAGGGATACGTCCCGTCACATTATCATCACGCCACCGTTGTAATCTTCTGGTCAACTCTACGCCAAAGGTCGCCCGGc                     >  2:15477267/1‑137 (MQ=255)

                                                                                                                         ctgccagAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTcc              >  1:9674116/7‑137 (MQ=255)

                                                                                                                            aTCGAGCACCGCCCGCGCAGCACACGATCTGGAAGGCGCTG‑CACTACTTGAGGCGTACGCCCCATCACATTACCACCACGCCACCGTCGTAACCTGCCGGCCAAATCCATGCCAAAAGTCACCCGGCATGCTCCGcc           >  2:507299/1‑137 (MQ=255)

                                                                                                                               gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCNCATTATCATCACGCCACTGTCGTAATCTGCCGGCCAAATCTATGCCACAGGTCAACCGGCATGCTCCGCCTGc        >  1:17390492/1‑137 (MQ=255)

                                                                                                                               gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  1:12513503/1‑137 (MQ=255)

                                                                                                                               gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTAACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  2:3427273/1‑137 (MQ=255)

GGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTG‑CACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344174‑4344443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`