Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,223,0130CA34.1% ‑1.4 / 19.0 44L428F (TTG→TTT) PP_4603 CDS (transposase)
Reads supporting (aligned to +/- strand):  ref base C (1/28);  new base A (0/15);  total (1/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATGGCG  >  AE015451_phaM1/5222913‑5223142
                                                                                                    |                                                                                                                                 
gaggggTTGGGTGATCTCTCATAAAAAAAAATGCAACCCGTTTTTTTCTAAGAACTCAAGTGGCCACCAGTTGATACCCCAGTTTCTGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCg                                                                                               <  1:11595034/134‑1 (MQ=1)
gaggggCTGGGGGATCTCTCATAAAACAAAATGCAACCCGTTGTTTTCTAAGCACTCAAGTGGCCACCAGTTGATACCCCATTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCg                                                                                               <  2:1450750/134‑1 (MQ=1)
gaggggCTGGGGGATCTCCCACCAAAAAAAAGGAAACCCATTGTTTTCGAAGCACCCAAGCGGCCACCAGTGGATACCCCAGTTTCTGGGTTTTGAGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCg                                                                                               <  1:2971989/134‑1 (MQ=1)
                tctaaccaaacaaaATGCAACCCATTGTTCTCTAAGCACCAAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                                               <  1:14342229/131‑1 (MQ=1)
                tcccatcaAACAGACTGCACCCCGTTTTTTTCTAATCCCCCAATTGGCCACTGTTGGACACCCAGGTTTTTGGTTTTGGAGAAGCAAGCCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                                               <  1:1101707/134‑1 (MQ=0)
                tcccatcaAACAAACTGCAACCCATTGTTTTCGAAACACTCAAGCGACCACCAGTTGATACCCAAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                                               <  2:8318806/134‑1 (MQ=1)
                tcccatcaAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGAGACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                                               <  2:4665270/134‑1 (MQ=1)
                tcccaccaaacaaaATACACCCCATTTTTTTCGAAACACTAAGGTGGCCACCGTTTGATCCCCCATTTTCTGGGTTTGGAGAAAAAAATCCCGGACCTGCCGGTCTTGGATTCTGGTTTCGAACTCCTCAAGACCtt                                                                               <  1:7249825/131‑1 (MQ=0)
                tcccaacaaacaaaATGCAACCCGTTGTTGTCGAAGAACCAAAGCGGCCACCGGTTGATACCCCCTTTTCCGGGCTTGGCGAAGCAAATCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                                               <  1:9669017/131‑1 (MQ=1)
                cttcctcaAAAAAACTAAAACCCATTGTTTTAGAAGCACTCAACCGGCCACCAGTCGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCTGGACCTGCCGGTCTTGGATTCTGTTGTCGAACTCCTCAAGACCtt                                                                               <  2:15859363/132‑1 (MQ=1)
                ctccaccaaaaaaaAGGCAACCCGTTTTTTTCGAAGAACTCAAGCGGCCACAAGTGGATACCCCATTTTCTGGGTTTGGAGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                                               <  1:15042181/131‑1 (MQ=1)
                   caccaaaaaaaATGCAACCAATTGTTTTCTAAGCACTCAAGCGGCCACCGGTTGAGACCCCAGTTTCCGGGCTTTGCGAATCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGtt                                                                            <  2:5706484/134‑1 (MQ=1)
                      cacacaaaATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCaa                                                                         >  1:3958743/4‑137 (MQ=1)
                                        tttttttCTAAGCACTCAATCGACCACCGTTTGACACCCCATTTTCCGGGCTTTGCGAAAAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTgg                                                       <  1:3254547/134‑1 (MQ=1)
                                          tttgtCTAAGCACTCAGGTGGCCACTAGTTGACCCCCCAGTTTCCTGGCGTTGCGGAGCAAAGCCCGTACCTGCCTGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGtt                                                     <  2:1124424/133‑1 (MQ=1)
                                          tttctCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGtt                                                     <  2:4690702/133‑1 (MQ=1)
                                          tttctCTAAGCACTCAAGCGGCAACCAGTTGAGACCCCAGTTTCCGGGCTTGGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGtt                                                     <  1:6586776/133‑1 (MQ=0)
                                          gttgtttAAGCACTCAAGCGGCCACCATTTGATACCCCAGTTTCTGTGTTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTGCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGtt                                                     <  2:8446395/131‑1 (MQ=1)
                                           tttttgaaGCACTCAGGCGGCCACCGGTTGATCCCCCGTTTTCCGGTCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTa                                                    <  1:10210213/131‑1 (MQ=1)
                                              tcgaaGCACTAAAGCGGCCACCGGTTGATACCCCAGTTTCCGGGTTTTGCGAAACAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                                                 <  1:10524991/134‑1 (MQ=1)
                                              tccaaGCACTCAGGCTGCCACAAGTTGACACCCCATTTTCCGGGCTTTGAGAAACAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                                                 <  2:1227978/134‑1 (MQ=1)
                                              ccgaatcACCCAAGCGGCCACCGGTTGATACCCCGGTTTCCGGGTTTTGCGAGGCAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                                                 <  1:11080453/131‑1 (MQ=1)
                                                     acccAAGCGGCCACCATTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTa                                          <  1:1719754/134‑1 (MQ=1)
                                                     acccAAGCGGCCACCATTTGACACCCCATTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTa                                          <  1:9796607/134‑1 (MQ=0)
                                                         aatcGGCCACCAGTTAATACCCCAGTTTCCGGGCTTTGCGAAAAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACAcc                                      <  1:7463212/134‑1 (MQ=0)
                                                               ccatcggTTGATCCCCCCTTTTCTGGGCTTTGAGAAGAAAGGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGt                                <  1:5207563/131‑1 (MQ=1)
                                                                 accgggtGATACCCCGGTTTCCGGGCTTTGCGAAGAAAATCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGc                              <  1:9323303/131‑1 (MQ=1)
                                                                      ttgaccccccATTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACt                         <  1:1359113/131‑1 (MQ=0)
                                                                      ttaaTACCCCATTTTCCGGGCTTTGCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACt                         <  1:6456159/134‑1 (MQ=0)
                                                                      ttaaTACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACt                         <  1:17037218/134‑1 (MQ=1)
                                                                           acctcggTTTCCGGGCTTTGCGAAACAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATgc                    <  1:1482149/131‑1 (MQ=0)
                                                                           acccaggTTTCCGGGCTTTGTGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATgc                    <  1:16678968/131‑1 (MQ=0)
                                                                                          tttggCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATg     <  1:13324230/133‑1 (MQ=1)
                                                                                          tttggCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATg     <  1:5432517/133‑1 (MQ=1)
                                                                                          tttggCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATg     <  1:2017271/133‑1 (MQ=0)
                                                                                          tttggCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATg     <  1:148506/133‑1 (MQ=0)
                                                                                          tttggCGAAAAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATg     <  1:9663435/133‑1 (MQ=0)
                                                                                          tttggCGAAAAAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATg     <  1:4788297/133‑1 (MQ=0)
                                                                                           ttggCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATgg    <  1:12524365/134‑1 (MQ=1)
                                                                                           ttggCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATgg    <  1:16633507/134‑1 (MQ=1)
                                                                                           ttggCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATgg    <  1:1543964/134‑1 (MQ=1)
                                                                                           ttggCGAAACAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATgg    <  1:2236115/134‑1 (MQ=1)
                                                                                           ttggCGAAAAAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATgg    <  1:5912057/134‑1 (MQ=0)
                                                                                             tgagAAACAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATAgcg  <  1:7555448/134‑1 (MQ=0)
                                                                                                    |                                                                                                                                 
GAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATGGCG  >  AE015451_phaM1/5222913‑5223142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑