Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM11,210,8300GT29.8% 1235.5 / 277.0 611intergenic (+20/+17)ytnA CDS (Uncharacterized amino acid permease YtnA)/PP_1060 CDS (putative Glutamate synthase_ large subunit)–/–
Reads supporting (aligned to +/- strand):  ref base G (109/320);  new base T (182/0);  total (291/320)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 614 total aligned reads displayed.

GATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAT  >  AE015451_phaM1/1210694‑1210965
                                                                                                                                         |                                                                                                                                       
   aCGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCgg                                                                                                                                      <  1:467314/137‑1 (MQ=255)
    cgcgTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGc                                                                                                                                     <  2:14676618/137‑1 (MQ=255)
       gtgtGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTTTGGcccc                                                                                                                                  >  2:13424492/1‑137 (MQ=255)
       gtgtGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACA‑TCTTGCGGcccc                                                                                                                                  >  2:7328432/1‑137 (MQ=255)
        tgtgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCa                                                                                                                                 <  2:3342388/137‑1 (MQ=255)
        tgtgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCa                                                                                                                                 <  2:17117156/137‑1 (MQ=255)
              ccTGTACATCTGCCCAGCGTTCTTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTAACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTtc                                                                                                                           <  2:15870277/137‑1 (MQ=255)
              ccTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTtc                                                                                                                           <  1:18509748/137‑1 (MQ=255)
                    cATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGTAG‑ATTTGCGGCCCCAGCGTTCTCTCAg                                                                                                                     >  2:16635010/1‑137 (MQ=255)
                    cATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CTTTGCGGCCCCAACGTtctctccg                                                                                                                     >  2:14402802/1‑135 (MQ=255)
                    cATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑ATTTTTGGCCCCAACATtctctccg                                                                                                                     >  1:18300161/1‑135 (MQ=255)
                     aTCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGGG‑ATTTGTGGCCCCAACGTtttctccaa                                                                                                                    >  1:11781897/1‑134 (MQ=255)
                     aTCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CTTTTTGGGCCCAACATtctctccag                                                                                                                    >  1:4094321/1‑134 (MQ=255)
                     aTCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAG‑GTTTTTGGGCCCAACGTTTTCTCCgg                                                                                                                    >  1:8816226/1‑137 (MQ=255)
                        ggCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGgccg                                                                                                                 <  2:16210625/137‑1 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCNCAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAG‑CTTTTTGGTCCCCGCGTTTTCTCCAgccgcc                                                                                                               >  1:17421977/1‑137 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CTTTTTGGCCCCAATGTTCTCTCAAgccgcc                                                                                                               >  2:5121479/1‑137 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGTGGCCCCCACGTTCTCTCCGgccgcc                                                                                                               >  2:17907299/1‑137 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTTTGGCCCCAGCGTTCTCTCCGgccgcc                                                                                                               >  2:6366533/1‑137 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTTTGGCACCAACGTTCTCCCCGgccacc                                                                                                               >  2:833216/1‑137 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CCTTTTGGCCCCAACGTTCTCTCAGgtcgcc                                                                                                               >  2:1525042/1‑137 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CCTTTCTGCCCCCACCTTCTCTCCGgccgcc                                                                                                               >  2:5871595/1‑137 (MQ=255)
                          cccGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACA‑CCTTTTGGCCCCCACGTTCTCTCAAgccgcc                                                                                                               >  1:7019070/1‑137 (MQ=255)
                            cGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAG‑CTTTTCGGTCCCCACGTTCTCTCAGtcctcccc                                                                                                             >  1:1064643/1‑135 (MQ=255)
                             ggCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGGG‑CTTTTTGGCCCCCACGTTTTCTCCGgccgccccc                                                                                                            >  1:9202595/1‑134 (MQ=255)
                             ggCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACt                                                                                                            <  2:10482484/137‑1 (MQ=255)
                              gCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CTTTGTTGTCCCCACATTCTCTCAAGCCACCCCtt                                                                                                           >  2:10610190/1‑137 (MQ=255)
                               cGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAGAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTTTGGCCCCCGCGTTCTCTCCCGCCGCCAATTc                                                                                                          >  1:18443498/1‑137 (MQ=255)
                               cGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAA‑CGTTTTGGCCCCAACGTTTCCTCCGGCCCCCACCTc                                                                                                          >  1:10894431/1‑137 (MQ=255)
                               cGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGTGGCCCCAGCGTTCTCTCAGGCCGCCACTTc                                                                                                          >  2:17982998/1‑137 (MQ=255)
                                  tCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCt                                                                                                       <  1:10010139/137‑1 (MQ=255)
                                  tCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCt                                                                                                       <  1:11278145/137‑1 (MQ=255)
                                  tCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCt                                                                                                       <  1:6362764/137‑1 (MQ=255)
                                  tCCTGGTGTTGCTGACAGTGCTGGATGACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGGGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGTGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCt                                                                                                       <  1:8406683/137‑1 (MQ=255)
                                      ggTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGc                                                                                                   <  1:16433383/137‑1 (MQ=255)
                                          ttGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCt                                                                                               <  2:5618771/137‑1 (MQ=255)
                                               gACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CTTTTCGGTCCCAGCGTTCTCTCAGGCCGCCACTTCTCTGTGTGGCTCGaaa                                                                                          >  2:11841109/1‑137 (MQ=255)
                                               gACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGaaa                                                                                          <  1:16860748/137‑1 (MQ=255)
                                               gACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGAAG‑CTTTTTGGCCCCCACGTTCCCTCCCACCGCCACTTCGCCGTGCGGCTctcaa                                                                                          >  1:13562364/1‑133 (MQ=255)
                                                aCAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAAc                                                                                         <  1:7848879/137‑1 (MQ=255)
                                                aCAGGGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTATAGCCTCCTGACCTGACGTTGGGCCG‑CTTTGCGGCCCCAGCCTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAAc                                                                                         <  1:18151923/137‑1 (MQ=255)
                                                   gTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGt                                                                                      <  1:13082256/137‑1 (MQ=255)
                                                     gCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CCTTTTTGCCCCCACATTCTCTCCAGCCACCACCTCTCTTTGCGGCTCGAAACTGttc                                                                                    >  2:3660642/1‑135 (MQ=255)
                                                     gCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAG‑TTTTTTGGCCCCCACGTTCTCTCAGGCCGCCAACTCTCTGTGTGGTTCTAAACTGTcc                                                                                    >  2:13721214/1‑137 (MQ=255)
                                                       tGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAG‑CTTTTTGGGCCCCACGTTCTCTCCGGCCTCCCCCTCTCTTTGTGGCTCGAAAACGTcctc                                                                                  >  1:15142664/1‑135 (MQ=255)
                                                       tGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTTCTGCCCCCACTTTCTCTCCCGCCCCCCCCTCCCTTTTTGGTTCGCAACCGTCCGc                                                                                  >  2:16328745/1‑137 (MQ=255)
                                                       tGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTGCGGCCCCCGCGTTTTTTCCCGTCGCCACTTCGCTGTGTGGTTCGAAACTGTCCGc                                                                                  >  1:13889551/1‑137 (MQ=255)
                                                         tATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGccc                                                                                <  1:16938808/137‑1 (MQ=255)
                                                         tATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGccc                                                                                <  2:9302277/137‑1 (MQ=255)
                                                            tACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCggg                                                                             <  2:18445379/137‑1 (MQ=255)
                                                            tACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCggg                                                                             <  1:12163478/137‑1 (MQ=255)
                                                                cGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACA‑CTTTGTGGCCCCCACGTTCTCCCCAGCCCCCCCTTCGCTGTGTGGCTCGAAAACGTCCACCCGGGACAg                                                                         >  2:14831050/1‑137 (MQ=255)
                                                                 gTTCCGCCTGGCGCCGAAAGACGCACCAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTTCTGCCCCCACGTTCTCTCCCACCCCCCCCTCTCTGTGCGGCTCTAAAACGTCCACCCGGGcaaag                                                                        >  1:2392983/1‑133 (MQ=255)
                                                                 gTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACA‑CTTTGTGGTCCCAGCTTTCTCTCAGGCCACCCCCTCGCTGTTTTGTTCTAAAATGTCCACCCgggtccgt                                                                        >  2:14008870/1‑132 (MQ=255)
                                                                     cGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGcc                                                                    <  2:7954938/137‑1 (MQ=255)
                                                                      gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCT‑CTTTTTGGTCCCCGCGTTTTCCCCGGCCCCCCCCCCACTGTGCGGCTCTAAAATGTTCGCCCAGGCCAGGTGccc                                                                   >  2:2499479/1‑136 (MQ=255)
                                                                      gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CTTTGCGGCCCCCGCGTTCTCTCCAGCCGCCCATTTGCTGTGCGGGTCGGAAATGTTCCCCCGAGCCAATTTCca                                                                   >  2:7633356/1‑137 (MQ=255)
                                                                      gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAGCCTTTTTGTCCCCA‑CCTTCTCTCCCGCCACCCCCCCCCTTTTCTGCTCTAAACCGTCCCCCCCCGCCAATTGccc                                                                   >  1:6661402/1‑136 (MQ=255)
                                                                      gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTTCGGGCCCAGCGTTTTCTCAGGCCGCCACTTCTCTGTGTGGATCGAAACCATCCCCCCAGGACAGTTGccc                                                                   >  2:12163537/1‑136 (MQ=255)
                                                                      gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACG‑CTTTGCGGCCCCAACATTCTCTCCGGCCAACCCCTCGCTGTGCGGCTCGAAAATGTCCGCCCAGGCCAATTGccc                                                                   >  2:4147767/1‑136 (MQ=255)
                                                                        cTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCcaca                                                                 <  2:17468284/137‑1 (MQ=255)
                                                                        cTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCcaca                                                                 <  1:12560734/137‑1 (MQ=255)
                                                                             gcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCcgc                                                            <  1:13236429/137‑1 (MQ=255)
                                                                             gcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCcgc                                                            <  1:17607704/137‑1 (MQ=255)
                                                                                  aaGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CTTTTCGGCCCCCACGTTCTCTCCAGCCGCCACCTCGCCGTTTGGCTCGAAAACGTCCGCCCCGGCCAATTGCCACATCCCCGaaca                                                       >  2:17404098/1‑135 (MQ=255)
                                                                                           aaGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGcc                                              <  1:14937124/137‑1 (MQ=255)
                                                                                           aaGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGcc                                              <  1:3757295/137‑1 (MQ=255)
                                                                                             ggTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGt                                            <  2:18806799/137‑1 (MQ=255)
                                                                                                gtgACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCa                                         <  1:5056822/137‑1 (MQ=255)
                                                                                                  gACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCAcc                                       <  1:9631972/137‑1 (MQ=255)
                                                                                                    cGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑TTTTTTGGCCCCAACGTTTTCTCCGGCCGCCCCTTTACTTTTTGGGTCGAAACTGTCCGGCCGGGCCAGTTTTCACATCCCAGAAGAGAACTGGCCTTTCACCTc                                     >  2:13017431/1‑137 (MQ=255)
                                                                                                    cGAGTACAGCCTCCTGACCTGACGTGGGGGCG‑CCTTTTGGCCCCAACATTCTCTCCAGCCGCCCCCTCTCTGTGCGGCTCGAAACCGTCCCCCCAGGCCAGTTTTCACACCCTCGAATCGAACCGACCGTTcccccc                                     >  2:6641227/1‑135 (MQ=255)
                                                                                                    cGAGTACAGCCTCCTGACCTGACGTGGGGACG‑ATTTGTGGCCCCAACGTTTTCTCAAGCCACCCCTTCGCTGTGTGGCCCTAAACTGTTCTCCCAGGCCAGTTTCCCCACCCGCGAATCGAACTGGCCGTTTAcccc                                     >  2:15066492/1‑135 (MQ=255)
                                                                                                     gAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCg                                    <  1:3454453/137‑1 (MQ=255)
                                                                                                       gTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGcc                                  <  2:1019461/137‑1 (MQ=255)
                                                                                                        tACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCg                                 <  2:2848714/137‑1 (MQ=255)
                                                                                                          cAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGt                               <  1:5469580/137‑1 (MQ=255)
                                                                                                           aGCCTCCTGACCTGACGTGGGGGCG‑CTTTGTGGACCCAGCGCTTTCTCCGGCCGCCACTTCGCCTTGCGGGTCGAAAATGTCCGCCCGGGGCCGGTGCCACATCCTCGAAGAGAACTGGCCGGTCAACTCGCCAGtt                              >  2:4697180/1‑136 (MQ=255)
                                                                                                             cctcctGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTcag                            <  2:9715989/137‑1 (MQ=255)
                                                                                                             cctcctGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTcag                            <  1:4344488/137‑1 (MQ=255)
                                                                                                               tcctGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTcagca                          <  1:2918546/137‑1 (MQ=255)
                                                                                                                cctgacctgaCGTGGGGGCA‑CTTTTCGGCCCCAGCATTCTCTCAGGCCCCCCCTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGAACAACTGTCAAACCCACGAATAAAAATGGACGTTCACCCCGCCAGTcagcag                         >  2:11939435/1‑137 (MQ=255)
                                                                                                                  tgacctgacGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCt                       <  1:15653790/137‑1 (MQ=255)
                                                                                                                  tgacctgacGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCt                       <  2:11352745/137‑1 (MQ=255)
                                                                                                                   gacctgacGTGGGGGCA‑CTTTTCTGCCCCAACGTTCTCTCCAGACGCCAACTCGCCGTGCGGGCCGAAACTGGCCGCCCGAGACAGGTGCCAAATCCACGAACAGAAATTGCCGTTCACCTCGCCGGTCCGCAGATc                      >  2:4260818/1‑137 (MQ=255)
                                                                                                                     cctgacGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCAc                    <  1:15541851/137‑1 (MQ=255)
                                                                                                                          cGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGc               <  2:16911919/137‑1 (MQ=255)
                                                                                                                            tGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCtt             <  1:8142355/137‑1 (MQ=255)
                                                                                                                            tGGGGACG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCCCCTCGCTGTGTGGCTCGAAAATGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACCGGCCGTTCCCCTCTCCGGTTAGCAGCTCACCCGGCtt             >  2:18194477/1‑137 (MQ=255)
                                                                                                                             ggggCCG‑CTTTGCGGCCCCAGCGTTCTCTCATGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTc            <  2:11594230/137‑1 (MQ=255)
                                                                                                                             ggggCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTc            <  2:1251286/137‑1 (MQ=255)
                                                                                                                             ggggCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGAGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTc            <  1:14791660/137‑1 (MQ=255)
                                                                                                                                 ccG‑CTTTGTGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGa        >  2:8848648/1‑137 (MQ=255)
                                                                                                                                  gggttttGTGGTCCCAGTGTTCTCTCGGGTCGTCACTTCGCTGTGTGGCTCGAAACTGTCCGCCTGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGACGGTCAGCAGCTCACCTGGCTTCAAgg        >  2:8479221/5‑136 (MQ=255)
                                                                                                                                   g‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAac      <  1:14168901/137‑1 (MQ=255)
                                                                                                                                        tGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCTGGTCAGCAGCTCACCTGGCTTCAAGAACACAt  <  1:2063187/137‑1 (MQ=255)
                                                                                                                                        tGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt  <  1:594114/137‑1 (MQ=255)
                                                                                                                                        tGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt  <  1:16009597/137‑1 (MQ=255)
                                                                                                                                        tGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt  <  2:2091056/137‑1 (MQ=255)
                                                                                                                                        tGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt  <  2:9040986/137‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCG‑CTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAT  >  AE015451_phaM1/1210694‑1210965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑