Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 1,713,060 A→G T2A (ACC→GCC)  tsaB CDS (endopeptidase) →

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM11,713,0600AG99.7% 3057.0 / ‑4.5 752T2A (ACC→GCC) tsaB CDS (endopeptidase)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (448/302);  minor base T (1/1);  total (449/303)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 757 total aligned reads displayed.

TGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712927‑1713192
                                                                                                                                     |                                                                                                                                    
   cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATGCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:6921855/137‑1 (MQ=255)
   cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:9742111/137‑1 (MQ=255)
   cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:3044522/137‑1 (MQ=255)
   cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:3197191/137‑1 (MQ=255)
   cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:4144966/137‑1 (MQ=255)
   cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  2:12369738/137‑1 (MQ=255)
   cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTAGATGGCAAccc                                                                                                                                >  1:8628224/1‑137 (MQ=255)
     cACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctg                                                                                                                              >  2:18302724/1‑137 (MQ=255)
     cACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCATTTGTAGGCCGCACAGCGCCCCCTCCGGACACCACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCTATGGCCACCctg                                                                                                                              >  2:11542123/1‑137 (MQ=255)
        cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctg                                                                                                                           <  1:13050635/137‑1 (MQ=255)
        cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctg                                                                                                                           <  1:7609373/137‑1 (MQ=255)
        cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctg                                                                                                                           <  2:16623385/137‑1 (MQ=255)
         tCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTgg                                                                                                                          <  1:10727583/137‑1 (MQ=255)
         tCGACAACGGCCCGCTTGAGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTgg                                                                                                                          <  2:15792733/137‑1 (MQ=255)
                  gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                 <  1:13839770/137‑1 (MQ=255)
                  gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                 <  2:16155568/137‑1 (MQ=255)
                   cccGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATAc                                                                                                                >  2:12978165/1‑137 (MQ=255)
                    ccGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATAcc                                                                                                               >  1:13371630/1‑137 (MQ=255)
                     cGCTTGCGGGCCGTTGTCGTTTTTCGGCCGCACAGCGCCCCCCCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCCTTCGCACCTGGATAACCGTTCGATGGCCACCCTGTTGGCCCTGGATAACg                                                                                                              >  1:10297001/1‑137 (MQ=255)
                     cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACCGCGCCCCCTCAGGGCACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCg                                                                                                              >  2:5992680/1‑137 (MQ=255)
                     cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCg                                                                                                              >  2:2580387/1‑137 (MQ=255)
                      gCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGc                                                                                                             >  2:8826904/1‑137 (MQ=255)
                       cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCATGACACAACTCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  2:9336502/137‑1 (MQ=255)
                       cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  2:15063518/137‑1 (MQ=255)
                       cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCGCATGACACAACGTCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGCTgccgcc                                                                                                            <  2:17539042/137‑1 (MQ=255)
                              gCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAg                                                                                                     >  2:3131301/1‑137 (MQ=255)
                                cGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGcc                                                                                                   >  2:2342986/1‑137 (MQ=255)
                                    gTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACACTGCTGGCCCTGGATACCGCCACCGAAGCCTGtt                                                                                               <  2:15562265/137‑1 (MQ=255)
                                           gTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTcgc                                                                                        >  2:14627145/1‑137 (MQ=255)
                                            tAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTcgcg                                                                                       <  2:11057824/137‑1 (MQ=255)
                                             aGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCgcgc                                                                                      >  1:11516435/1‑137 (MQ=255)
                                                  gCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                 >  1:14591922/1‑137 (MQ=255)
                                                      aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  1:14093422/1‑137 (MQ=255)
                                                      aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:17023213/1‑137 (MQ=255)
                                                      aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             <  1:6717552/137‑1 (MQ=255)
                                                      aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAACGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  1:10392382/1‑137 (MQ=255)
                                                        cgcCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGAc                                                                           <  2:14333294/137‑1 (MQ=255)
                                                         gcCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACg                                                                          <  2:653967/137‑1 (MQ=255)
                                                           ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  2:4126997/1‑137 (MQ=255)
                                                           ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  1:3578400/1‑137 (MQ=255)
                                                           ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  2:13237319/1‑137 (MQ=255)
                                                            cccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCa                                                                       >  1:2491272/1‑137 (MQ=255)
                                                               tCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCCCTTCTTCATTCGCACCGGGATACCCGTTCGATGGCCACCTTGCTGGCCCGGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAgg                                                                    <  1:6183225/137‑1 (MQ=255)
                                                                cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  1:12664765/137‑1 (MQ=255)
                                                                cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  1:11145763/137‑1 (MQ=255)
                                                                cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   >  2:17679304/1‑137 (MQ=255)
                                                                cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  1:16421248/137‑1 (MQ=255)
                                                                cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  1:5356740/137‑1 (MQ=255)
                                                                cAGGACACAACGCCGCTGATGCTCTATACTGCCGCTCTTCTTCATTCGCACCTGGATACCCGCTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  2:9766686/137‑1 (MQ=255)
                                                                    acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACTCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  2:16234404/137‑1 (MQ=255)
                                                                    acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATNCCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:15882203/137‑1 (MQ=255)
                                                                    acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCTAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:4560773/137‑1 (MQ=255)
                                                                    acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:9557287/137‑1 (MQ=255)
                                                                         aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  1:901020/137‑1 (MQ=255)
                                                                         aCGCCGCTGCTGCTCTATACTGCCGCTCTCTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  1:6482893/137‑1 (MQ=255)
                                                                           gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  1:7548933/1‑137 (MQ=255)
                                                                                tgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAg                                                   >  2:7573241/1‑137 (MQ=255)
                                                                                tgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCAATACGAg                                                   >  2:369884/1‑137 (MQ=255)
                                                                                   tgctCTATACTGCCGCTCTTCTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTg                                                <  2:4358363/137‑1 (MQ=255)
                                                                                        tataCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGTTGATccc                                           <  1:3446586/137‑1 (MQ=255)
                                                                                        tataCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATccc                                           <  1:376537/137‑1 (MQ=255)
                                                                                        tataCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATccc                                           <  2:11804857/137‑1 (MQ=255)
                                                                                        tataCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATccc                                           <  1:11977034/137‑1 (MQ=255)
                                                                                            cTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCgcgc                                       <  1:12457589/137‑1 (MQ=255)
                                                                                             tGCCGCTCTTTTTCATTCGCACCTGGATACCNGTTCGATGGCCACCCTGNTGGCCCTGGATACCGCCACCGAAGCCTGNTCCGTCGCGCTGCTNCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgca                                      >  1:15338408/1‑137 (MQ=255)
                                                                                             tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGANGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgca                                      <  1:18173921/137‑1 (MQ=255)
                                                                                             tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgca                                      >  1:14842807/1‑137 (MQ=255)
                                                                                              gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATTACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  2:3442994/137‑1 (MQ=255)
                                                                                              gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  1:8347502/137‑1 (MQ=255)
                                                                                              gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  1:7949172/137‑1 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:8103525/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:14237343/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:16045526/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:5668072/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:8547206/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:3191953/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:9301082/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:17681305/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:2114342/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:10108966/1‑137 (MQ=255)
                                                                                                  ctctTTTTCATTCACACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGACGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:6923526/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGCTACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:16517632/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:5779996/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:4872897/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:1363861/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:13956158/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:17275641/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:18767091/1‑137 (MQ=255)
                                                                                                     tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTACATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:17532283/1‑137 (MQ=255)
                                                                                                       tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  1:18771100/1‑137 (MQ=255)
                                                                                                       tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  1:14491253/1‑137 (MQ=255)
                                                                                                       tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:7917625/1‑137 (MQ=255)
                                                                                                       tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCAATACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:4968164/1‑137 (MQ=255)
                                                                                                                 aCCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGctgctg                  <  1:7219991/137‑1 (MQ=255)
                                                                                                                        tACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCgatga           <  1:18260994/137‑1 (MQ=255)
                                                                                                                           ccGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCa        <  1:10107865/137‑1 (MQ=255)
                                                                                                                           ccGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCa        <  2:478401/137‑1 (MQ=255)
                                                                                                                           ccGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCa        <  1:15558199/137‑1 (MQ=255)
                                                                                                                              ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  2:6423690/1‑137 (MQ=255)
                                                                                                                                cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   <  2:1988288/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712927‑1713192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑