Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 353,067 A→G intergenic (+34/+84) hisF CDS (Imidazole glycerol phosphate synthase subunit HisF) → / ← cbcV CDS (choline _ betaine _ carnitine ABC transporter ‑ ATP binding subunit) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1353,0670AG100.0% 1435.2 / NA 381intergenic (+34/+84)hisF CDS (Imidazole glycerol phosphate synthase subunit HisF)/cbcV CDS (choline _ betaine _ carnitine ABC transporter ‑ ATP binding subunit)–/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (197/184);  total (197/184)

Only 100 of 383 total aligned reads displayed.

TGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGA  >  AE015451_phaM1/352934‑353200
                                                                                                                                     |                                                                                                                                       
 gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGCGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCgggga                                                                                                                                     >  1:10251585/1‑135 (MQ=255)
 gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCa                                                                                                                                     >  1:6546548/1‑137 (MQ=255)
 gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCa                                                                                                                                     >  1:12293419/1‑137 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCNAGTACACAGTGCCGGAGGCCAAGGCATACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   >  1:18163939/1‑137 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   <  2:13769833/137‑1 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   <  1:5198932/137‑1 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   >  2:10984182/1‑137 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   >  2:16790173/1‑137 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                   >  1:13069294/1‑137 (MQ=255)
    aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGTAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                  <  2:5583064/137‑1 (MQ=255)
    aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                  <  1:7811380/137‑1 (MQ=255)
        cgGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGc                                                                                                                              <  2:13100409/137‑1 (MQ=255)
         ggTGCTGGCGGCCAGCATTTTCCATTTTGGCGAGTACACAGTGTCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATTGTCGTTCGCTGATCCATTGACTGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGcg                                                                                                                             <  1:13466040/137‑2 (MQ=38)
         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCt                                                                                                                             >  2:1872095/1‑137 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  2:16900435/137‑1 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  2:7846685/137‑1 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  1:2818504/137‑1 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                            <  2:10180147/137‑1 (MQ=255)
              tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCcac                                                                                                                        >  1:5023563/1‑137 (MQ=255)
               ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCcaca                                                                                                                       <  1:15535920/137‑1 (MQ=255)
               ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCcaca                                                                                                                       <  2:14151159/137‑1 (MQ=255)
                 cggcNAGCATCTTCNACTTTGGCGAGTACACNGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGAGCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCNCTTCGCGGGCACGCCCGCTCCCACAga                                                                                                                     <  2:6878696/137‑2 (MQ=255)
                     cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTg                                                                                                                 <  1:7515318/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGGGTACACAGTGCCGGAGTCCAAGGCCTACATTGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCTGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               <  2:12005717/137‑1 (MQ=38)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               <  2:11404840/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               <  1:11936612/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               <  1:16196917/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               <  1:2081270/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               <  2:14622774/137‑1 (MQ=255)
                        cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTttgttg                                                                                                              >  2:15544067/1‑137 (MQ=255)
                        cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTg                                                                                                              >  2:9765927/1‑137 (MQ=255)
                        cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTg                                                                                                              >  1:8281674/1‑137 (MQ=255)
                        cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTg                                                                                                              >  1:15660854/1‑137 (MQ=255)
                        cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCAGTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCAAAGGTTTGCTg                                                                                                              >  2:16012037/1‑137 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACATAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  1:3744734/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGATGCCAAGGCCTACATGGCTTCGCGCGGGGTCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:5826355/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATTGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:7333723/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:11725000/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:10262904/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  1:12958234/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  1:9065848/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:5281540/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:6455313/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:6703834/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:7152375/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  1:6957185/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:8255914/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:8986075/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  1:10031905/137‑1 (MQ=255)
                         aTCTTCCACTGTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  1:2640252/137‑1 (MQ=255)
                         aTCGTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGTGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  2:315907/137‑1 (MQ=255)
                           cTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTgaga                                                                                                           >  2:17903939/1‑137 (MQ=255)
                           cTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTgaga                                                                                                           <  2:12487760/137‑1 (MQ=255)
                           cTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTgaga                                                                                                           <  1:4232893/137‑1 (MQ=255)
                               cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGACCGCTCCCACAGGTTTGCTGAGAtctc                                                                                                       >  1:3283775/1‑137 (MQ=255)
                                       cGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               <  1:1690180/121‑1 (MQ=255)
                                        gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGACCGCTCCCACAAGGTTGTTTAGATCTCTGTGGGAGc                                                                                              >  1:11421299/1‑137 (MQ=38)
                                        gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGAACGCCCACCCCCCCAGAGTTGTTTCGATCTCTTTTGGAgg                                                                                              >  1:15040417/1‑136 (MQ=255)
                                                   gCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCAAAGGGTTGCTGAGAGCTCTGTGGGAGCGGGC‑GTGccca                                                                                  >  2:2108743/1‑136 (MQ=38)
                                                    ccGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGGACACCCCCCCCCCCAAGTTTTCTTAGATTTCCGTGGGGGAGGGC‑GTTccccc                                                                                 >  1:3967511/1‑135 (MQ=255)
                                                     cGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAAGTTTGCTGAGACCCCTGTGGGAGCGGGC‑GTGCCcacg                                                                                >  1:1062297/1‑137 (MQ=38)
                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGTACACCCACCCCCAAAAATTTTCTTAGATCCTCGTGGGAGAAGGC‑GGGcccccaaa                                                                              >  1:97593/1‑132 (MQ=255)
                                                        aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                               >  1:14545328/1‑104 (MQ=255)
                                                               ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCAAAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCACGAAGGGCcgca                                                                      >  1:18609127/1‑136 (MQ=255)
                                                                gCCTACGTGGCTTCGCGAGGGATCGTTGTTCGTTGATCCATGGACTGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCAAAGGTTTGTTGAGACCTCTTGGGGGGCGGGC‑GTGCCCGCGAAGGGCCGCGa                                                                     <  2:14889286/137‑1 (MQ=25)
                                                                 ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGAGaa                                                                    >  2:9618551/1‑137 (MQ=255)
                                                                 ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCCACAGGTTTGCTGGGATCTCTGTGGGAGCGGGC‑GTGCCCCCGAAGGGCCCCGaa                                                                    >  1:16610142/1‑137 (MQ=38)
                                                                       tGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGGTTTGTTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑TGGcc                                                             >  2:17202906/1‑137 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCTCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTTCCACCGGGTTGCTGGGGTTTCCGTGGGAGCAGGC‑GGGCCCACAAAGGGGCGCGAAG‑CGGtccccagt                                                       >  2:1894551/1‑133 (MQ=19)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCTCTCCCACAGGTTTGCTGAGATCTCCGTGGGGACGGGC‑GTTCCCGCAAAGGGCCTAGAAG‑CGGCCCCAAtt                                                       >  1:13572640/1‑137 (MQ=37)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAAGTTTTCTTAGATCTCTTTGGGAGCGGGC‑GTGCCCACAAAGGGCCGCAAAA‑CAGCCCCAAtt                                                       >  1:18264727/1‑137 (MQ=37)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAAGTTTGGTGAGGTCTCTGTGGGAACGGGC‑GTGCCCCCGAAGGGACGCGAAG‑CTGGCCCAAtt                                                       >  1:14047456/1‑137 (MQ=37)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAAGTTTGGTGAGATCTCCGTGGGGGGGGGC‑GTGCCCGCAGAGGGCCGCGAAA‑AGGACCCAAtt                                                       >  1:8564677/1‑137 (MQ=25)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTTCTGAGATCTCTGGGGGAGCGGGC‑GTGCCCACGAAGGGCCACGAAG‑CGGCCCCaact                                                       >  1:18437429/1‑135 (MQ=38)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGTTGAGGTCTCTGTGGGGGCGGGC‑GTGCCCACGAAGGGCCGCGAAA‑AGGCCCCaact                                                       >  2:3782251/1‑135 (MQ=37)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGGTGAGATCTCTGTGGGAGCGGGC‑GTTTCCGCGAAGGGCCGCGAAG‑CGGCCCCAAtt                                                       >  2:12133859/1‑137 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                             <  1:7157551/85‑1 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGT‑GTGTCCGCGAAGGGCCGCGAAG‑CGGCCCCaact                                                       >  2:3577466/1‑135 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAA‑CGGCCCCAAtt                                                       >  2:13559791/1‑137 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCACGAAG‑CGGCCCCAAtt                                                       >  2:2722861/1‑137 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGGTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCAAAA‑CGGCCCCAAtt                                                       >  2:4834431/1‑137 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAAGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCAAAAGGCCACAAAA‑CAGCCCCaact                                                       >  1:12582860/1‑135 (MQ=37)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACTCCCACAAGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑CGGCCCCAAtt                                                       >  2:4682603/1‑137 (MQ=255)
                                                                              gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCCCCCACAGGTTTGCTGAGATCTCTGTGGGGGTGGGC‑GTGACCGCGAAGGGCCGCGAAA‑AGGACCCAATTg                                                      >  1:9408810/1‑137 (MQ=37)
                                                                              gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCACTTCCAAAGGGTTGTTTAAAACTCCGGGGGGGTGGGA‑GTGTCCCCGAAAAGACGCAAAGAAGGCCCCaacg                                                       >  1:11014215/1‑135 (MQ=12)
                                                                               tgtgggATCGTCGTTCGTGGGTCAATTAACCGGGGTTGCTTGGCAGCCTTTCGCGGGCACGCCCGTTCCCAAAGGTTGGCTGAGATCTCTGTGGAAGCGGGC‑GTGCCCACGAAGGGCCGCGAAG‑CGGCCCCAATTGc                                                     <  2:11121426/134‑1 (MQ=25)
                                                                                gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑CGGCCCCAATTGCt                                                    >  2:5923876/1‑137 (MQ=255)
                                                                                gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGGTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCAGCGAAG‑CGGCCCCAACTGCt                                                    >  1:17286420/1‑137 (MQ=255)
                                                                                          tgtTCGCGGCCCCAGTGCCCGGGTCGGTTTTGCATCCTTTTCCGGTCACTCCCGCTCCCACGGTTTTTCTGGGATCTTTTTGGGAGGGGGT‑TTGCCCGCGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCAACCGTgg                                          <  1:17898128/136‑1 (MQ=255)
                                                                                                   aTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGACCGCTCCCACAAGTTTTCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCAAGAAAGGCCGCGAAG‑CGGCCCCAATTTCTTCACCCGTGGTTCTTTcca                                 >  1:4484180/1‑136 (MQ=37)
                                                                                                       aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACCGGTTTGTTGGGATCCCTGTGGGAGAGGGC‑GTGCCCACAAAAGGCCACGAAG‑CGGCCCCAATTGCTTCAACCATGGTTCTTGccaaaaa                             >  2:10717010/1‑132 (MQ=25)
                                                                                                        gtGACCGGGGTTTTTTTGAAACCCTTTTGGGGCACCCCCCCCCCCAATGGTTTGGTGGGATTTTTTTGGGGGGGGGCTTTCCCCGCGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagca                             <  1:6062241/136‑1 (MQ=255)
                                                                                                            ccGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGGGTTGCTTAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑TGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAAcgcg                        >  2:10813789/1‑137 (MQ=38)
                                                                                                                 tctgctTTGCAGCCCTTCGCGGGCACGCCTGCCCCTACATGTTGTCGGAGACTCCTGTTGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑CGGCCTCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGTTa                   <  2:13326521/136‑1 (MQ=25)
                                                                                                                   tgctTTGCAGCCCTTCGCGGGCACGCCCACTCCCACAGGTTTTTTGAGGTCTCTGTGGGAGCGGGC‑GTGCCCGCGAAAGGACGCAAAG‑CGGCCCCAATTTTTTCAACCGTGGTTCTTTCCAAGCAACGCGTGGTaaa                 >  1:7994519/1‑137 (MQ=21)
                                                                                                                      tttGCAGCCCTTCGCGGGCACGCCCGCTCCCACAAGTTTGTTGAGATCTCTGTGGGAGCGGGC‑GTGCCCACGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCCACCATGGTTCTTGCCGAGCAACGCGTGGTAAAACt              >  2:10422681/1‑137 (MQ=37)
                                                                                                                       ttGCAGCCCTTCTCGGGCACGCCCGCTCCCACAGGTTGGCTGAGATCTCGGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCGGCGCGGGGTAAGGCTc             <  2:17574071/137‑1 (MQ=38)
                                                                                                                          catcccTTCGCGGCCACGCCCGCTCCCATGGTTTTGTGGGTGTTTTTGTGGGGGTGGGC‑GTTCCCGCGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGt          <  1:1768229/134‑1 (MQ=25)
                                                                                                                               cTTCTCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGTGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCAACCTTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATccc     <  2:4141703/137‑1 (MQ=255)
                                                                                                                                  cgcgGGCACGCCCGCTCCCAAAGGTTTGCTGAGGTCTCTGTGGGAGCGGGC‑GTGCCCGAGAAGGGCCGCGAAG‑CGGACCCAACTGCTTCAAACGTGGTTCTTGCCGAGCAGCGCTTGGTAAAGCCTGGTATCCCCGa  >  2:5765414/1‑137 (MQ=37)
                                                                                                                                     |                                                                                                                                       
TGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGC‑GTGCCCGCGAAGGGCCGCGAAG‑CGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGA  >  AE015451_phaM1/352934‑353200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑