Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 539,294 A→C T558P (ACC→CCC)  rpoB CDS (DNA‑directed RNA polymerase subunit beta) →

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1539,2940AC100.0% 3007.6 / NA 737T558P (ACC→CCC) rpoB CDS (DNA‑directed RNA polymerase subunit beta)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (355/382);  total (355/382)

Only 100 of 754 total aligned reads displayed.

CAGCCAGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAG  >  AE015451_phaM1/539161‑539426
                                                                                                                                      |                                                                                                                                        
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGcccc                                                                                                                                       >  2:10169566/1‑137 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGcccc                                                                                                                                       >  1:11711785/1‑137 (MQ=255)
cagccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGcccc                                                                                                                                       <  1:7244605/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTa                                                                                                                                   <  2:14770862/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTa                                                                                                                                   <  2:1800160/137‑1 (MQ=255)
     agcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTAt                                                                                                                                  <  1:8066407/137‑1 (MQ=255)
        tGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGc                                                                                                                               <  2:14577933/137‑1 (MQ=255)
          tCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTTACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCg                                                                                                                             >  1:4700932/1‑137 (MQ=255)
          tCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCg                                                                                                                             >  2:9331657/1‑137 (MQ=255)
          tCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCg                                                                                                                             >  2:11676669/1‑137 (MQ=255)
               gTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCgtgtgt                                                                                                                        <  1:12992648/137‑1 (MQ=255)
               gTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCgtgtgt                                                                                                                        <  2:17386076/137‑1 (MQ=255)
                  cATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCTTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTTTGcc                                                                                                                     >  1:10002109/1‑137 (MQ=255)
                   aTGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGccc                                                                                                                    <  1:7748921/137‑1 (MQ=255)
                    tGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCg                                                                                                                   >  1:17918544/1‑137 (MQ=255)
                      gACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGAt                                                                                                                 <  2:1707320/137‑1 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                                >  2:2016879/1‑137 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                                >  2:2709243/1‑137 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                                >  1:9170777/1‑137 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                                >  1:11030701/1‑137 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                                >  1:5761068/1‑137 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                                <  2:2677044/137‑1 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                                <  2:16521889/137‑1 (MQ=255)
                        ccAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCg                                                                                                               >  1:647275/1‑137 (MQ=255)
                        ccAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCg                                                                                                               >  1:13922198/1‑137 (MQ=255)
                              caacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAccc                                                                                                         <  1:12544427/137‑1 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAg                                                                                                   >  2:5163541/1‑137 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAg                                                                                                   >  1:6838352/1‑137 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAg                                                                                                   >  1:3810516/1‑137 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAg                                                                                                   >  2:16827049/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                              >  1:2221628/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                              >  1:9684849/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                              >  1:17137586/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                              >  1:13319720/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCAACTATGGCCGTGTGTGCCCGATCGAGACCCATGAAGGTCCg                                                                                              >  2:3605661/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTACGTGACGTACA‑CCCGCCCCCCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                              >  2:13633311/1‑137 (MQ=255)
                                          ggAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGa                                                                                             <  2:8296133/137‑1 (MQ=255)
                                              aTTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACAt                                                                                         >  1:18429866/1‑137 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                        <  1:17448042/137‑1 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                        <  1:18789400/137‑1 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                        <  2:8709946/137‑1 (MQ=255)
                                                  cccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGt                                                                                     <  1:6120333/137‑1 (MQ=255)
                                                  cccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGt                                                                                     <  1:15783030/137‑1 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                    >  2:6342939/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                    >  1:10348641/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                    >  2:13827816/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                    >  2:11000502/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                    >  2:15099131/1‑137 (MQ=255)
                                                    cacaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCt                                                                                   >  2:13667147/1‑137 (MQ=255)
                                                     acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTg                                                                                  >  2:3922474/1‑137 (MQ=255)
                                                           gccgcGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAAc                                                                            <  1:16154830/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACG‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAAACCCCTGAAGGTCCAAACATCGGTATGATCAACTcac                                                                        >  2:10504036/1‑135 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                        <  2:4801766/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                        <  2:18354128/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                        <  2:15453835/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                        <  1:1352261/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                        >  1:8610783/1‑137 (MQ=255)
                                                                     cGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTggcgg                                                                  <  2:764429/137‑1 (MQ=255)
                                                                     cGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGACCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTggcgg                                                                  <  2:2894059/137‑1 (MQ=255)
                                                                              cccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGccc                                                         <  2:12499009/137‑1 (MQ=255)
                                                                              cccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGccc                                                         <  2:8992327/137‑1 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTTAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                        <  2:3362599/137‑1 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                        >  2:611164/1‑137 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                        >  1:11810847/1‑137 (MQ=255)
                                                                                cTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGc                                                       <  1:11279688/137‑1 (MQ=255)
                                                                                   gCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑acc                                                <  1:3031521/137‑1 (MQ=255)
                                                                                   gCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGGACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑acc                                                <  2:12902816/137‑1 (MQ=255)
                                                                                     ggTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑accaa                                              >  1:15622436/1‑137 (MQ=255)
                                                                                     ggTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑accaa                                              >  2:435765/1‑137 (MQ=255)
                                                                                     ggTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑accaa                                              >  1:15159555/1‑137 (MQ=255)
                                                                                      gTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑accaac                                             >  2:5874615/1‑137 (MQ=255)
                                                                                          gACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGt                                         <  2:6782445/137‑1 (MQ=255)
                                                                                           aCCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTa                                        >  2:13178206/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGGAGGTCCGAACAGCGGTCTGATCAACTCCCTTGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTcc                                <  2:17708930/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTcc                                <  1:3424395/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTGCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTcc                                <  2:6969730/137‑1 (MQ=255)
                                                                                                    cGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCt                               <  1:5448344/137‑1 (MQ=255)
                                                                                                     gTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTg                              <  2:15838075/137‑1 (MQ=255)
                                                                                                     gTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTg                              <  1:14303157/137‑1 (MQ=255)
                                                                                                      tGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTgg                             >  2:16003284/1‑137 (MQ=255)
                                                                                                        ccGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGaa                           <  1:12829216/137‑1 (MQ=255)
                                                                                                        ccGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGaa                           <  1:3742740/137‑1 (MQ=255)
                                                                                                         cGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGaaa                          >  2:18755391/1‑137 (MQ=255)
                                                                                                         cGGCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGaaa                          >  1:15400380/1‑137 (MQ=255)
                                                                                                          ggCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAg                         >  1:16237514/1‑137 (MQ=255)
                                                                                                          ggCTTCGAAGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAg                         >  1:5095042/1‑137 (MQ=255)
                                                                                                                 aaGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTTGAAAGCCCGTAc                  <  2:7858886/137‑1 (MQ=255)
                                                                                                                 aaGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTAc                  <  1:9899569/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  1:16625584/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  2:11345501/137‑1 (MQ=255)
                                                                                                                   gTCCGTGACGTACACCCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  2:14115820/137‑1 (MQ=255)
                                                                                                                    tCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAACCAGTACGGCTTCCTGGAAAGCCCGTa                   <  1:1893456/137‑1 (MQ=255)
                                                                                                                    tCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACcgc               <  1:2709243/137‑1 (MQ=255)
                                                                                                                    tCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACcgc               <  2:11905566/137‑1 (MQ=255)
                                                                                                                    tCCGTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACcgc               <  2:8476376/137‑1 (MQ=255)
                                                                                                                       gTGACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCgtg            <  2:410222/137‑1 (MQ=255)
                                                                                                                         gACGTACA‑CCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCgtggt          >  2:12165351/1‑137 (MQ=255)
                                                                                                                           cGTACA‑CCCGCCCCACTATGGCCGTGTGTGTCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTga        <  1:14631946/137‑1 (MQ=255)
                                                                                                                                  cccGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGCAAGCCCGTACCGCGTGGTgaaggaag  >  1:12424040/1‑137 (MQ=255)
                                                                                                                                  cccGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTgaaggaag  >  1:8730064/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CAGCCAGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACA‑CCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGC‑‑‑‑ACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAG  >  AE015451_phaM1/539161‑539426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑