| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,499,497 | 1 | . | C | 79.7% | 567.3 / 218.9 | 266 | intergenic (+74/‑90) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base . (26/28); new base C (155/57); total (181/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.22e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | AE015451_phaM1 | 1,499,498 | 0 | T | G | 85.1% | 532.2 / 21.8 | 266 | intergenic (+75/‑89) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base T (25/10); new base G (145/55); total (181/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 283 total aligned reads displayed.
TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑CACGGCTTTGCCGGTG‑T‑T‑T‑C‑GGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAC > AE015451_phaM1/1499363‑1499631 ||| aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGGAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAGGCGCCACGGTGCATGGC‑ACCAGCCTTTTCGGTG‑t‑t‑tgtg > 1:15865049/1‑134 (MQ=255) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCAGCGGTGTATGGC‑ACCGGGTTTGCCGGTG‑t‑t‑tgcggga > 2:4583512/1‑131 (MQ=37) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTTTCGGTG‑T‑TCG‑C‑GGGCAc‑g‑cc > 2:2721418/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCGCCGCGGTGCACGGC‑ACCAGATTTTCCAGTG‑T‑TCT‑C‑GGGCAc‑g‑cc > 2:14381565/1‑137 (MQ=25) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAACAGGCGCCGAGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAc‑g‑ccc > 2:8668969/1‑136 (MQ=25) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAACGCCACGGTGCCCGGC‑ACCCGCTTTTTCTGTGTT‑T‑T‑C‑GGGCAc‑a‑cccc > 1:7210195/1‑135 (MQ=255) ggCCATTAAGATCCGGAACGAAGCTTTGGCACCACAACAGGACTGAGGCTTCCAAACGGTGGGAGCGGGCGTGCCCACGAAACGGGCACTGCGGTGCATGGC‑ACTGTTTTTGCTGGTG‑T‑T‑TGG‑GGGCAc‑g‑cccgctc < 2:17977249/137‑6 (MQ=12) ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTccc > 2:2146854/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGC‑ACCGGATTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCcac > 2:10770619/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCcac > 2:8386896/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCGAAACAAGAGCCACGGGGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGTACAC‑CCGCTCCcac > 1:17694237/1‑137 (MQ=21) cAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCAAGAAACAGGAGCCGCGGGGCATGGC‑ACCGGATTTGCCAGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGa > 1:2601955/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGAGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGTCGGTG‑T‑TCG‑C‑GGGCACAC‑CCGCTCCCACAGACTGATTGc > 2:5387511/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGTCGGTG‑T‑TCG‑C‑AGGCACAC‑CCGCTCCCACAGCCTGACTGc > 2:7622155/1‑137 (MQ=18) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGACCGCGAAAAAGGCACCGCGGTGCATGGC‑ACCAGCTTTGGCGGTT‑T‑TCA‑C‑GGGCCC‑GCCCGATCCCACAGGCTGATTGc > 2:11411147/1‑137 (MQ=255) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCCGACTTT‑CCGGTG‑T‑TCG‑C‑GGGCCCAC‑CCACTCCCACAGACTGATTGCCAt > 2:16131614/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGGCCGCGAAACAAGAGCCGCGGTGTATTGC‑ACCAGCTTTGCCGGTG‑T‑T‑TGC‑GGGACC‑GCCCACTCCCACAGAATGAGTGACAtt > 2:14493285/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCACTGC‑ACCAGCTTTTCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCCCTCCCACAAAATGATTGCCAtt > 2:8098455/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑T‑CGC‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCact > 2:15842965/1‑135 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCACGAAACAGGCGCCGCAGTGTATTGT‑ACCAGCTTTTTCGGTG‑T‑TCT‑C‑GGGCACAC‑CCACTCCCACCAACCGCTTGCCAtc > 1:3504729/1‑136 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGAGCCACAGTGCACGGC‑ACCAGATTTGCCAGTG‑T‑T‑TGC‑GGGCAC‑GCCCACTCCCACAAAATGATTGCCact > 2:6169870/1‑135 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCACGAAACAAGCACCACGGTGTCTGGC‑ACCAACTTTTCCGGTGTT‑T‑T‑T‑GGGCACAC‑CCACTCCCCCAAAATGATTGCCAtt > 1:5430246/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGACTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCCTTGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGATCCCACAGAATGATTGCCAtt > 2:15030849/1‑137 (MQ=21) ttttGGCACCACGGGGGGATTGGGGTTGCAAAACGGGGGGAGTGGGTGTGCCCGAGAAACGGGCCCTGTGGTGCGGGGC‑ACTGGTTTTGCGGGTG‑T‑T‑TGT‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGc < 1:4187958/135‑1 (MQ=255) ccTTGACCCCACAACAGGCCTGAGGCTGCCAAACGGTGGGGAGGGGCGTACCCGCAAAAAGTACCCCGCGGTACGTAAC‑ACCGGCTTGGCTGGGG‑TCA‑G‑T‑GGGCAC‑GCCCGCTCCCACAGACTGATTACCATTAAGc < 2:3107489/137‑1 (MQ=255) ttGGCACCACAGCGGGACTGAGGCTGCAAAAAGGTGGGAGCGGGTGTGCCAGAGAAACGGGCGCTGTGGTGCGGGGC‑ACCGGTTTTGCTGGTG‑T‑T‑CGC‑GGGAAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:10918848/137‑1 (MQ=255) tgggaaccacAGAAGGAGTGGGGCGACAAAAGGGGGGGAGAGGGTGTTCCCGCGAAACAGGCTCCGTGGGGCATGGC‑ACCGGTTTTGCTGGTG‑T‑TCG‑T‑GGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 1:9022621/132‑1 (MQ=255) tGGCACCACAGCGGGATTGAGGTTGCCAAATGGTGGGAGCGGTTGTTCCCGCGAAACGGGCGCCGTGGGGCAGGGA‑ACTGGCTTTGCTGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 2:1833063/137‑1 (MQ=16) tGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGTGGGCTTGCCCGCGAAACGGGCGCCGCGGTGCATGGC‑ACTTGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 2:17134834/137‑1 (MQ=21) gaaccacAGAAGGATGGGGGCTACAAAATGGGGGGAGCGGGTGTTCCCGAGAAACAGGCGCCGTGGGGCGTGGC‑ACTGGTTTTTCCGGTT‑T‑TCG‑C‑GGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:9341229/135‑1 (MQ=255) gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑T‑CGA‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:16713815/137‑1 (MQ=21) acaccccAGCAGGACTGGGTTTGCCAAACGTGGGGATCGGTTTTCCCTGCGAAACGGGCGCCGTGGTGCGTGCC‑ACCGTTTTTACCGGTG‑T‑TCG‑C‑GGGCAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:6619821/136‑1 (MQ=255) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCTCGAAACAGGCGCCCCGGTGCCTGGC‑CCCGGCTTTTCCGGTG‑T‑TCG‑C‑GGGTCC‑GCCCGCTCCCACCAAATTATTGGCCTTCAACGGGCCCAt > 1:18222235/1‑137 (MQ=255) agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCATGAAACAGGCGCCGCGGTGCATGGC‑ACCAGCTTTTCCAGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGAATGATTGCCACTAAGGGGGACTATCGCTaaa > 2:2312529/1‑135 (MQ=18) gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTTCACGGC‑ACCGGCTTTGCCGGTT‑T‑TCG‑C‑GGGCCC‑GCCCGCTCCCACCGACTGATTGCCATTAAGCGGGCCTATCGCCACAGTGAca > 2:9880844/1‑136 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGACGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑T‑GGGCAC‑GCCCGCTCCCAAAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcac > 2:7252958/1‑135 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCCTGGC‑CCCGGCTTTGCCGGTG‑T‑T‑TGC‑GGGGAC‑GCCCGCTTCCACAAACTGATTGCCATTAAGCGGGCCTATTGCTACAGCGACGGccc > 2:6722324/1‑137 (MQ=37) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑T‑TGC‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTATCGCTACAGTGACGGccc > 2:12508449/1‑137 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCACGGTGCATGGC‑ACCGGATTTGTCGGTG‑T‑T‑TGC‑GGGCACAC‑CCACTCCCACAGACTGGTTTCCCTTCAGCGGAACTGTTGCTGCAGTGAAGGgcc > 1:18470448/1‑137 (MQ=9) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGTAGTGCATGGC‑ACCAGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTTCCACAGAATGATTGCCATTAAGCGGGACCACCGCTACAGTGACGGCCCGtttt > 2:11839400/1‑137 (MQ=17) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGtttt > 2:9333810/1‑137 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑T‑CGC‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTCTCGCCACAGTGACGGCCCGtttt > 2:13888989/1‑137 (MQ=18) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCACGGC‑ACCGGCCTTTCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAAAACGATTGACCTTAAGCGGGCCTATCGCTCCAGTGACGGCCCGttttt > 2:6925553/1‑137 (MQ=17) tttGGGAGCGGGCGTCCCCGCGAAATAGGCGTCGTGGGGCAGGGC‑ACTGGTTTTGCTGGTG‑T‑TCG‑C‑GGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 1:5784409/135‑1 (MQ=255) gggggGCGGGCTTGCCCGCGAAACAGGCGCCGTGGTGCATGGC‑ACTGTCTTTGCTGGTG‑T‑TCG‑T‑GGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 1:13768348/136‑1 (MQ=255) ggAGCGGGTGTGCCCGCGAAACGTTTGCCGCGGTGCAGGGC‑ACCGGTTTTGCCGTTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 1:12398725/137‑1 (MQ=25) gAGCGGGCGTGCCCGCGAAACAAGCGTCCCAGTGCCTGGC‑CCCAGCTTTGTCCGTG‑T‑TCG‑C‑CGGCACAC‑CCGCTCCCACCAACTGATTGTCATTAAGCGGGCCTCCCTCTCCAGTGGCGGACCGTTTTTTGTTGCCt > 2:400945/1‑137 (MQ=9) agtgggCGTACCCGAGAAACGGGCGCCGTGGTGAATGGC‑ACTGTTTTTGCTGGTT‑T‑TCG‑G‑GGGAAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc < 2:1195313/134‑1 (MQ=255) gCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCCTGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGACCCCACAAAATGGTTGCCATTAAGCGGGGCTATCGCTACAGTGAAGGCCCGGTTTTTGTTGCCTCg > 2:10089338/1‑137 (MQ=18) gggTGTGCCTGAGAAACAGGCGCCGCGGTGCGTGGC‑ACCGGCTTTGCCGGTG‑T‑T‑CGC‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt < 2:16479936/137‑1 (MQ=25) gCGTGCCCGCGAAACGTGCGCTGCGGTGCATGGC‑ACTGTTTTTGCTGGTG‑T‑T‑TGT‑GGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 2:10368299/137‑1 (MQ=255) tgcgAAACAGGTTCCGTGGGGCAGGGC‑ACTGTTTTTGTGGGTG‑T‑T‑TGG‑GGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:12828264/136‑1 (MQ=255) cgcgAAACAGGTGCCGCGGTGCATGGC‑ACCGTCTTTACCGTTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:1977617/137‑1 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTCCCGGTG‑T‑T‑C‑CTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:15905412/137‑1 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGAC‑ACCGGCTTTTCCGTTG‑T‑TCG‑C‑GGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:15655868/137‑1 (MQ=255) cgcaAAACGGGCGCCGCGGTGCATGCC‑ACCGTTTTTTCCGTTG‑T‑TCT‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:18116023/137‑1 (MQ=255) cgaaaaaCAGGCGCCGTGGTGCGTGGC‑ACTGGTTTTGCTGGTG‑T‑TCG‑C‑GGACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:18785069/133‑1 (MQ=255) agagAAACGGGCGCTGGGGTGCAGGGC‑ACTGTTTTTGCTGGTG‑T‑TCG‑C‑GGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:14902472/134‑1 (MQ=255) gcgAAACAGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:17732192/137‑1 (MQ=25) aaaGGCGCCGCGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 2:17704421/135‑1 (MQ=37) ctccgcGGTGCATGGC‑ACCGGCTTTTCCGGTG‑T‑T‑CGT‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:6490289/135‑1 (MQ=37) cgctgcGGTGCATGGC‑ACCGGCTTTGCTGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACATACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:17446809/137‑1 (MQ=25) cgccgtGGTGCGTGAC‑ACCGTCTTTGCCGGTG‑T‑T‑CGC‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:7882999/137‑1 (MQ=37) cgccgcGGTGCGTGGC‑ACGGGTTTTGCAGGTG‑T‑T‑CGT‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:10425024/137‑1 (MQ=37) cgccgcGGTGCATGGC‑ACCGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 2:10681687/1‑137 (MQ=37) cgccgcGGGGCATGGC‑ACCGGCTTGTCTGGTG‑T‑TCG‑C‑GGGAAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:13527620/137‑1 (MQ=255) cATGGC‑ACCGGCTTTGCCGGTG‑T‑T‑CGC‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:6417235/137‑1 (MQ=37) accGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 1:14210516/3‑137 (MQ=38) cGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 2:48268/1‑137 (MQ=38) cGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:16984923/1‑137 (MQ=38) cGGCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:8418263/1‑137 (MQ=38) ggCTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:438360/107‑1 (MQ=37) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCGTGACTACCTTAGAACGCTATCAAGCAGATCTg > 2:15637092/1‑137 (MQ=38) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:2152626/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:3657076/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:15668606/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:14339335/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:1260398/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:12067149/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:11478245/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:10052060/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:12616548/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:15471196/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:14727701/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:13319116/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:2714525/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:7480151/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:5531673/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:404536/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:290695/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCACGCAGATCTGaa > 2:13675086/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:18726152/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:1866242/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:13717972/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:1923103/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:10546976/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑C‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACG‑ATCCTGCCTCCCTTAGAACGCTATCAAGCAGATCTGaaa > 1:11209056/1‑137 (MQ=38) ttGCCGGTG‑T‑T‑CGC‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:10897406/1‑137 (MQ=39) tgttcgc‑GGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 2:13460277/131‑1 (MQ=255) tct‑C‑GGGCCC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc < 1:15480738/135‑1 (MQ=255) ||| TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGC‑CACGGCTTTGCCGGTG‑T‑T‑T‑C‑GGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAC > AE015451_phaM1/1499363‑1499631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |