BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	3,845,705	+G	intergenic (‑72/+26)	PP_3394 CDS (putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase) ← / ← PP_3395 CDS (Transcriptional regulator_ LysR family)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	3,845,703	1	.	G	95.0%	1666.1 / 71.9	439	intergenic (‑70/+28)	PP_3394 CDS (putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase)/PP_3395 CDS (Transcriptional regulator_ LysR family)	–/–
Reads supporting (aligned to +/- strand): ref base . (1/21); new base G (308/107); total (311/128)
Fisher's exact test for biased strand distribution p-value = 3.02e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 468 total aligned reads displayed.

GCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑GG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAA  >  AE015451_phaM1/3845567‑3845832

 cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑g‑c‑g                                                                                                                                     <  2:3178297/137‑1 (MQ=255)

  aTGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑gg                                                                                                                                    >  1:10151540/1‑137 (MQ=255)

  aTGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑gg                                                                                                                                    <  1:505390/137‑1 (MQ=255)

   tGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑ggg                                                                                                                                   >  1:9989654/1‑136 (MQ=255)

            gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCAt                                                                                                                        >  2:14101163/1‑137 (MQ=255)

              aGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTTTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGCTTC‑G‑CGGG‑CT‑GA‑TCCATCg                                                                                                                      <  2:10055600/137‑1 (MQ=255)

              aGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCg                                                                                                                      <  1:769754/137‑1 (MQ=255)

               ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGt                                                                                                                     <  1:6459402/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  2:66699/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  1:6656714/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  1:7476345/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  1:8027932/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  1:444019/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  1:8967011/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  2:13484471/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  1:18029883/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  2:17016300/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  2:5660032/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  1:15371533/137‑1 (MQ=255)

                gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTc                                                                                                                    <  2:18152010/137‑1 (MQ=255)

                  tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAg                                                                                                                  <  2:17902425/137‑1 (MQ=255)

                   ctGCAAGCCACCGCGCAGGCCGAATTAAGTGATTGTTATTGTTGTCATCGCGGTCTCTCTATCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGc                                                                                                                 >  2:4210327/1‑137 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTTTTATTGTTTTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:8847806/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  1:11623700/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:931367/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:4863232/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:3226026/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                >  1:9765990/1‑137 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  1:3791364/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:11138279/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:8205201/137‑1 (MQ=255)

                    tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:16015007/137‑1 (MQ=255)

                    tGCAAGCCATCGCACAGGCCGACTTCATTGATTGTTATTGTGGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGcc                                                                                                                <  2:8433219/137‑1 (MQ=255)

                     gCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCa                                                                                                               <  1:621180/137‑1 (MQ=255)

                       aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACg                                                                                                             >  1:3231025/1‑137 (MQ=255)

                       aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACg                                                                                                             >  2:7839851/1‑137 (MQ=255)

                          ccATCGCGCAGGCCGACTTCGTTGGTTGTGGTTGTTTTCATCGCGGTTTCTCTGTTTGAAGTGTTGCGCCAGACGAAGAGCTGGAGGATGGGAAGAAAAGACGTGTTTT‑GTG‑GG‑CG‑GG‑TCCGTTGTCAGCCACgggg                                                                                                          <  2:18961395/137‑4 (MQ=255)

                          ccATCACACAGCCCGACTTCATTGATTTTTATTTTTGTCATTACGGTCTCTCTGCCGGAGGCTTTTCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑TGGG‑CT‑GT‑CCCATCGTCAGCCACGATg                                                                                                          <  2:12308016/137‑1 (MQ=255)

                            aTCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGa                                                                                                        >  2:1468560/1‑137 (MQ=255)

                               gcgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCa                                                                                                     >  1:12712601/1‑137 (MQ=255)

                                 gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTACGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGc                                                                                                   >  1:10471569/1‑137 (MQ=255)

                                 gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGc                                                                                                   >  2:14155739/1‑137 (MQ=255)

                                 gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGc                                                                                                   >  1:2520572/1‑137 (MQ=255)

                                 gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGc                                                                                                   >  1:16692725/1‑137 (MQ=255)

                                 gcAGGCCGAATTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGc                                                                                                   >  2:11630794/1‑137 (MQ=255)

                                                  attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGcc                                                                                  >  1:16989993/1‑137 (MQ=255)

                                                    tgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCgg                                                                                >  1:18714099/1‑137 (MQ=255)

                                                       tatttttTTCATCTCGGTCTCTCTTTCTGAGGTGTGGCGCAAGACGAAGAGCGGGAGGACGGGCAGAAAAAACTGGTTTCGG‑G‑GG‑CG‑GA‑TCCGTCGTAAACAAGGAGGGATAAGCCCGCGCCCTGCCAGGCCGGTGc                                                                             <  2:12868311/137‑1 (MQ=255)

                                                          tgttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCCTCGTCCGCCACGATGGATCCGCCCGCGCCCTTCCAGGCCGGTGCACg                                                                          >  1:14897311/1‑137 (MQ=255)

                                                           gttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgc                                                                         >  1:5900194/1‑137 (MQ=255)

                                                                 aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaaa                                                                   >  1:7205510/1‑136 (MQ=255)

                                                                 aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaaa                                                                   >  1:5509072/1‑136 (MQ=255)

                                                                 aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAAc                                                                   >  2:13162734/1‑137 (MQ=255)

                                                                 aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCAAGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAAc                                                                   >  1:4301486/1‑137 (MQ=255)

                                                                   cgcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAg                                                                 >  1:16122761/1‑137 (MQ=255)

                                                                   cgcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAg                                                                 >  2:11905227/1‑137 (MQ=255)

                                                                           ctctGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCaa                                                         >  1:3984312/1‑137 (MQ=255)

                                                                                      tctttacgcCAGACGAAGATTTGGAGAAAGGACAAAAAGGACGTTTTTC‑G‑G‑GG‑GTGGA‑CCCATCGCAAACAACGGGGGATAACCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccga                                              <  2:5454176/131‑1 (MQ=255)

                                                                                      gCGTTGCGCCAAACGAAGAGCTGAATAACGAAAAAAAAAGCCTTTTTCC‑G‑C‑GG‑TT‑TACCCCCTCGTCACCAACGGGGGACCACCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccga                                              <  1:8628258/137‑1 (MQ=255)

                                                                                       cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAATCAGGCgccgag                                             >  1:10836411/1‑137 (MQ=255)

                                                                                       cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag                                             >  1:3513259/1‑137 (MQ=255)

                                                                                       cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag                                             >  1:15592801/1‑137 (MQ=255)

                                                                                       cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag                                             >  1:13840466/1‑137 (MQ=255)

                                                                                       cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCCGCCAAGCCAGTGCACAAAGCAACAGGTGGTCAAGCAGGCgccgag                                             >  1:2926896/1‑137 (MQ=255)

                                                                                       cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAACCACGATGGATCCGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag                                             >  1:1585902/1‑137 (MQ=255)

                                                                                        gTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc                                            >  2:15157195/1‑137 (MQ=255)

                                                                                           gcgcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagccga                                         >  2:10914766/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:13328068/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  2:4509612/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:9303295/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:8289197/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  2:2415958/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:13509644/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  2:6261617/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:13112216/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:12448581/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:17733666/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:2060853/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  2:9451696/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  2:9555269/1‑137 (MQ=255)

                                                                                             gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGATGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                       >  1:5776230/1‑137 (MQ=255)

                                                                                               cAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc                                     >  2:2954377/1‑137 (MQ=255)

                                                                                                 gACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCCCAATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCCGCAACAGGTGGTCAAGCAGGCGCCGAACCGACAGCga                                   >  1:10691798/1‑137 (MQ=255)

                                                                                                    tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGagag                                >  2:3650130/1‑137 (MQ=255)

                                                                                                    tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGagag                                >  2:10996830/1‑137 (MQ=255)

                                                                                                         gCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt                           >  1:5119146/1‑137 (MQ=255)

                                                                                                         gCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt                           >  2:17535676/1‑137 (MQ=255)

                                                                                                         gCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAACCGACAACGAGAGCgtgt                           >  2:14410302/1‑137 (MQ=255)

                                                                                                          ttGGATGATGGGAAAAAAAGCCGTTTTTC‑G‑C‑GGTCG‑GA‑TCCACCGCCGGCCACGATGGCTCATCCCGCGCCCTGCCAGGCCTATGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg                          <  2:16587648/136‑1 (MQ=255)

                                                                                                            ggATGATGGGCAGAAAAGACGTGTTTCTG‑G‑GG‑CT‑GC‑CCCGCTGTCAACCATGGTGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGcc                        <  2:13834154/137‑1 (MQ=255)

                                                                                                              aTGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCgg                      >  2:1550059/1‑137 (MQ=255)

                                                                                                               tGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGt                     >  2:7117922/1‑137 (MQ=255)

                                                                                                                gACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc                    >  1:14323490/1‑137 (MQ=255)

                                                                                                                gACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCACAGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACCGGTGGTCAAACAGGCACCGAACCGACAACGAGAGCGTGTGCCGGTc                    >  1:18260841/1‑137 (MQ=255)

                                                                                                                     gCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATTAGCCTGCGCCCTGCCAGGCCGGTGTACGCAGCAACAGGTGGTCAAGCAAGCGCCGAGCCGACAGCGAGAGCGTGTGCAGGTCGCGCa               >  2:12556817/1‑137 (MQ=255)

                                                                                                                     gCAGAAAAGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCa               >  2:13990033/1‑137 (MQ=255)

                                                                                                                         aaaaGACTTGTTTC‑G‑CGGG‑CT‑GC‑TCCATCTCCAACCACGAGGGACCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCa           <  2:10408789/137‑1 (MQ=255)

                                                                                                                           aaGACGTGTTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGa         >  2:5932424/1‑137 (MQ=255)

                                                                                                                                 tgtTTC‑G‑CGGG‑CT‑GA‑TCCCCCGTCCGCCACGATGGATCCACCCGCGCCCTGCCCTGCCGGTGCACGCAGCAAAAGGTGGTCAAGCAGGCGCCAAGCCAACAGCGAGAGCGTGTGCCGGTCGCGCATGTAGATGACGa   >  1:1174175/1‑137 (MQ=255)

                                                                                                                                 tgtTTC‑G‑CGGG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGa   >  1:276638/1‑137 (MQ=255)

GCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑GG‑CT‑GA‑TCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAA  >  AE015451_phaM1/3845567‑3845832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`