Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,889,6440GC32.6% 481.1 / 134.0 266intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
Reads supporting (aligned to +/- strand):  ref base G (118/35);  new base C (0/74);  total (118/148)
Fisher's exact test for biased strand distribution p-value = 4.34e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

AAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGT‑GGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGT  >  AE015451_phaM1/4889514‑4889778
                                                                                                                                    |                                                                                                                                      
              ggttgaaaaaCCCACAACCCAACTTGAGAAACAACTGGGGACCTTTT‑GGGGGCGGGCTCTCCCGCAAACCCCGGCAAATCTGTTGCCATCCACCGGTTTT‑TTTGTTTCGGGGGCGATCCCGCTCCCACAGGGGGCCa                                                                                                                    <  2:4462574/131‑1 (MQ=255)
               gacgaaaaacccaaaacccACCCTGA‑GAAAAACCTTGTACCCTTTGGGGACCGGCCTCTCCCGAAAACCCCGGAAAACCCGGTTCCATCCCCCGATTTG‑TCTTTTTCTCGGTAAACCCCGCTCCCACAGGGGGCCaa                                                                                                                   <  2:7714678/132‑1 (MQ=255)
                    aacccccACAACGCATTTTGGGGACCACCTTGGGACTTTGG‑GGGGGGGGTCTCGCCTGAAAACACTGGCAAAGTTGTTGCCCCCCACCGGTTTT‑TCGTCTTTGCGGGCAACCCCGCTCCCACAGGGGGCCAAAgctt                                                                                                              <  2:1337271/133‑1 (MQ=255)
                    aaaaCCAAAAACAAAATCTGAGAAAAAACTTGGACCCTTGG‑GGGAGCGGCTTCTCCTGAGAACACCGCAAACTCTGTTCCCGTCCCCTGGTTTT‑TCTGCTTCGGGGGAAACCCCGCTCCCACAGGGGGCCAAAgctt                                                                                                              <  2:4485579/137‑1 (MQ=255)
                      accccACACCACACTGTGAGAAACACCTTGGGACCTTGT‑GGGAGTGGTCCCCCCCGCAAACAACGAAAAGCCCGGTCCAACCCACTGGTGTT‑TTTGCTTCGCGGGAAATCCCGCTCCCACAGGGGGCCAAAgcttgc                                                                                                            <  1:12076437/135‑1 (MQ=255)
                        cccAAAACGCACTCGAAGGAACACTTTGGGACTTTTT‑GGGAGTGGTCTCGCCGGAGAACCCTGGAAAATCCGTTCCCCCCCCCTGGTTTG‑TTTGTTTTGGGGGAGATCCCGCTCCCACAGGGGGCCAAAgcttgctt                                                                                                          <  2:6364531/137‑1 (MQ=255)
                            caACGCACTCGGAGGAACAACTGGGGACCCTGT‑GGGAGCGGTTTCTCCCGGAAACACCGAAAAATCGGGGGCCGCCACCCGGTTTT‑TTTGTTTCGTGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                                      <  2:1543188/137‑1 (MQ=255)
                              ccccactCTGGGAAACACCTTGGGACCTGGT‑GGGAGCGGGTTCGCCCGAAAACACCGGAAAATCCGGGGCCATCCACGGGGTGG‑TTTGTTTTGTGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGata                                                                                                    <  2:11505615/131‑1 (MQ=255)
                              ccGCAATCGGAGAAACAACTTGGGACCTTGT‑GGGAGCGGTCTCTCCCGTAAACACCGGAAAATCTGGTGCCATCCACAGGGTTT‑TTTGTTTCGGGGGAGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGata                                                                                                    <  2:13435179/136‑1 (MQ=255)
                              acaaagtCTGGGAAACAACCCGAGACCTTGT‑GGGACAGTACCCACCCACAAACACCGGAAAAGCCGGTGACATCCCCCGGGTGG‑TCTTTTCTGTGTGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGata                                                                                                    <  2:11176645/131‑1 (MQ=255)
                                     gggagaAACAACTTGGGACCTGGG‑GGGAGGGGGTTCGCCGGGGAACACGGAAAAAGCGGGTGCCATCCACCGGTTTG‑TCTGCTTCGGGGGGGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAt                                                                                             <  2:1185268/135‑1 (MQ=255)
                                      tGAGAAACACCTTGGGACCCTGG‑GGGAGTGGTCTCGCCAGCGAAAACGGGAAAATCCGGTGCCACCCCCCGGGTTG‑TTTGTTTCGCGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAtt                                                                                            <  2:3560626/137‑1 (MQ=255)
                                       gagaAACAACTTGGGACCCTGT‑GGGAGGTGGCTCGCCTGCGAACACCGGAAAAGCTGGTGCCCCCCCCCGGTTTG‑TCTTTTTCGCGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTa                                                                                           <  2:16906965/137‑1 (MQ=255)
                                               atttgggACCTTGG‑GGGAGTGGGCCCACCCGAAAAAACGGGAAAATCCGG‑GACAACCAACAGGTTGTTCGGCTTTGTGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGATTGAGATATCACAATTACAAAGAgg                                                                                   <  2:17250471/133‑1 (MQ=255)
                                                   gggACCCTGT‑GGGAGCGGGCTCGACCGCGAACACCGGCAAAGCCAGTGCCACCCACCGAGTTG‑TCCGATTCGAAGGCGAGCCCGCTCCCAAAGGGGGCCAAAACTTTATTGAGACATCAAACTTAAAAAGAGGGAAt                                                                               >  2:8921697/1‑137 (MQ=21)
                                                     gacctggt‑gGGAGGGGGTTCGCCGGAAAACACCGGAAAAGTGGGGTCCATCCACTGGTTTT‑TTTGTTTTGTGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAttt                                                                             <  1:4181357/131‑1 (MQ=255)
                                                      actttgt‑gGGGGCGGGCTCGCCCGAAAACACTGGAAAAGCTGGTGCCATCCATCGGGTTG‑TTTGTTTCGTGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTtc                                                                            <  2:4021800/133‑1 (MQ=255)
                                                      acccgtt‑gGGAAAGGGCTCGCTCGCGAACACCGAAAAAGCCGTTGCCATCCACCGCGTTG‑TTGGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTtc                                                                            <  2:1809565/131‑1 (MQ=255)
                                                                                  acacCGGCAAAGCCGGTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCa                                                 >  2:1989421/1‑137 (MQ=255)
                                                                                  acacAGGAAAAGCTGGTGCCATCCATCGGGTTG‑TCTGTTTTGGGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCa                                                 <  1:18152144/137‑1 (MQ=255)
                                                                                   cacggacAAGGCTGGTGCAATCGACAGGGTGG‑TCTGTTTCCTGGGGGATCCCGCCCCCACAGGGGGCCAAAGCTTGCTGGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAc                                                <  2:15157826/131‑1 (MQ=255)
                                                                                   cacTGGCAAATCCGGGGCCATCCACCGGTTTG‑TCTGCTTTGTGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAc                                                <  2:8409166/137‑1 (MQ=255)
                                                                                       ggCAAAGCCGGTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                            >  1:5106342/1‑137 (MQ=255)
                                                                                       gacAAATCTGGTCCCCTCCCCCGATTTT‑TCTTTTTCGCGGTCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                            <  1:13321080/135‑1 (MQ=255)
                                                                                       gaaaaaGCTGGTACCATCCACCGGTTTG‑TCTGCTTCGCGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                            <  2:7557571/134‑1 (MQ=255)
                                                                                         cAAAGCCGGTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                          >  2:18263294/1‑137 (MQ=255)
                                                                                           aaGCCGGTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCAACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatca                                        >  2:16163448/1‑137 (MQ=255)
                                                                                             tctggTGCCACCCACCGAGTTG‑TCTGTTTCGGGGGAGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                      <  2:5551108/134‑1 (MQ=255)
                                                                                             cctggTGCCACCCACGGGGTTT‑TCTTCTCCGGGGGAAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                      <  2:11690816/134‑1 (MQ=255)
                                                                                             ccaggTCCCATCCACCGGGTTG‑TTTGCTCCCCGGCCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                      <  2:15845520/134‑1 (MQ=255)
                                                                                                ggTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGc                                   <  2:14777315/137‑1 (MQ=255)
                                                                                                 gTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa                                  >  1:1676028/1‑137 (MQ=255)
                                                                                                 gTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa                                  >  1:6727253/1‑137 (MQ=255)
                                                                                                  ttccATCCACCGGTTTT‑TTTGTTTCGCGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCaa                                 <  2:5074357/135‑1 (MQ=255)
                                                                                                    ccctCCACCGAGTTT‑TCTGTTTCGTGGGCGATCCCGCTCCCACAGTGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                               <  2:8066242/134‑1 (MQ=255)
                                                                                                    ccccccACCGGGTTT‑TCTGATTCGCGGACAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                               <  1:12415694/133‑1 (MQ=255)
                                                                                                    ccacccacCGGTTTG‑TCTGTTTCGTGGGAGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                               <  1:8919950/137‑1 (MQ=255)
                                                                                                    ccacccacCGGGTTT‑TTTGCTGCGCGGGAGAGCCAGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAATAGGTAATTTTTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                               <  2:13039655/137‑1 (MQ=255)
                                                                                                    ccATCCCCCGGTTTG‑TTTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                               <  1:7619299/137‑1 (MQ=255)
                                                                                                    ccATCCACCGATTTG‑TCTGCTTCGCGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                               <  2:12827567/137‑1 (MQ=255)
                                                                                                     cATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                              >  1:17111708/1‑137 (MQ=255)
                                                                                                     cATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                              >  1:4909238/1‑137 (MQ=255)
                                                                                                     cATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                              >  2:11743705/1‑137 (MQ=255)
                                                                                                     cATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                              >  1:4573119/1‑137 (MQ=255)
                                                                                                       tCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCa                            >  1:3876261/1‑137 (MQ=255)
                                                                                                          aCCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCCTGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                         >  2:14098705/1‑137 (MQ=255)
                                                                                                          aCCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                         >  1:1609953/1‑137 (MQ=255)
                                                                                                          aCCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                         >  1:14514701/1‑137 (MQ=255)
                                                                                                          aCCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                         >  1:4560257/1‑137 (MQ=255)
                                                                                                          aCCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                         >  1:3187630/1‑137 (MQ=255)
                                                                                                           ccGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGc                        >  2:16445340/1‑137 (MQ=255)
                                                                                                           ccGAGTTG‑TCTGCTTAGAGGACGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGc                        <  2:9584031/137‑1 (MQ=255)
                                                                                                               gTTG‑TCTGCTTCGCGGGCGAGCCCGCTTCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAACTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCAATGGCTCAg                    >  2:10442828/1‑137 (MQ=255)
                                                                                                               gTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                    >  1:6556301/1‑137 (MQ=255)
                                                                                                               gTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                    >  1:6885712/1‑137 (MQ=255)
                                                                                                               gTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                    >  1:7822263/1‑137 (MQ=255)
                                                                                                               gTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                    >  2:10984591/1‑137 (MQ=255)
                                                                                                               gTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                    >  2:15875588/1‑137 (MQ=255)
                                                                                                               gTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATAGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                    >  2:8589001/1‑137 (MQ=255)
                                                                                                                 tG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                  >  2:1379382/1‑137 (MQ=255)
                                                                                                                 tG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                  >  2:3960424/1‑137 (MQ=255)
                                                                                                                 tG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                  >  2:12225869/1‑137 (MQ=255)
                                                                                                                 tG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                  >  2:6629856/1‑137 (MQ=255)
                                                                                                                 tG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                  >  1:9217308/1‑137 (MQ=255)
                                                                                                                 tG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAACTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATAGGCAAGCCCATTGCTCAGaa                  >  2:4818220/1‑137 (MQ=255)
                                                                                                                     tttcTTCGCGGGCAACCCCGCTCCCANAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCt               <  1:18759448/134‑1 (MQ=255)
                                                                                                                      tGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg              >  1:16272065/1‑137 (MQ=255)
                                                                                                                      tGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg              >  1:8402767/1‑137 (MQ=255)
                                                                                                                       gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc             >  2:5254056/1‑137 (MQ=255)
                                                                                                                       gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc             >  2:2068508/1‑137 (MQ=255)
                                                                                                                       gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc             >  1:1566551/1‑137 (MQ=255)
                                                                                                                         ttCTCGGCCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaa           <  2:18785501/137‑1 (MQ=255)
                                                                                                                         ttCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaa           >  1:10389862/1‑137 (MQ=255)
                                                                                                                          tCGTGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa          <  1:16205221/137‑1 (MQ=255)
                                                                                                                          tCGCGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa          <  2:11545872/137‑1 (MQ=255)
                                                                                                                           tgtgggCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:14473416/134‑1 (MQ=255)
                                                                                                                           tgggtgCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:3340485/132‑1 (MQ=255)
                                                                                                                           tgcgggcggTCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATGACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:10000202/136‑1 (MQ=255)
                                                                                                                           tgcgGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:12282556/136‑1 (MQ=255)
                                                                                                                           tgagggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:13721890/134‑1 (MQ=255)
                                                                                                                           cgtgggCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:13466211/134‑1 (MQ=255)
                                                                                                                           cgtgggCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:12562210/134‑1 (MQ=255)
                                                                                                                           cgcgGGTGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:8841845/137‑1 (MQ=255)
                                                                                                                           cgcgGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:297759/137‑1 (MQ=255)
                                                                                                                           cgcgGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:17957250/137‑1 (MQ=255)
                                                                                                                           cgcgGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  1:1989421/137‑1 (MQ=255)
                                                                                                                           cgcgGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:15523157/137‑1 (MQ=255)
                                                                                                                           cgcgGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAGCCTGCaaaa         <  2:2508500/137‑1 (MQ=255)
                                                                                                                           cgcgGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:4314320/137‑1 (MQ=255)
                                                                                                                           cgcgGACGACCCCGCTCCCACAGGGGGCCAAAGCTTGCGGGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  2:9307647/137‑1 (MQ=255)
                                                                                                                           cgagggCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  1:11115921/134‑1 (MQ=255)
                                                                                                                           agcgGGAGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa         <  1:5880135/136‑1 (MQ=255)
                                                                                                                              gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTACTTTCTCATGGCTAAAATCGGTTTCATCGGCACCGACATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc      >  1:5073109/1‑137 (MQ=255)
                                                                                                                              gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                      >  2:10677850/1‑105 (MQ=255)
                                                                                                                              gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                      <  1:10677850/105‑1 (MQ=255)
                                                                                                                              gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc      >  1:4076808/1‑137 (MQ=255)
                                                                                                                              gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc      >  2:8310369/1‑137 (MQ=255)
                                                                                                                                gtacgCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg    <  2:6068186/133‑1 (MQ=255)
                                                                                                                                 cGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAgg   >  1:14570197/1‑137 (MQ=255)
                                                                                                                                  gAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTACAAAAGGCAGGt  >  2:15366917/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                      
AAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGT‑GGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTG‑TCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGT  >  AE015451_phaM1/4889514‑4889778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑