Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,199,6560GT32.3% 87.0 / 40.8 133intergenic (+31/+42)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–
Reads supporting (aligned to +/- strand):  ref base G (23/63);  new base T (33/8);  total (60/73)
Fisher's exact test for biased strand distribution p-value = 1.14e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

TGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGG  >  AE015451_phaM1/5199521‑5199783
                                                                                                                                          |                                                                                                                                 
tggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTc‑gc                                                                                                                                  <  2:9858590/137‑1 (MQ=255)
tggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTc‑gc                                                                                                                                  <  1:17682806/137‑1 (MQ=255)
 ggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTc‑gcg                                                                                                                                 <  2:8047672/137‑1 (MQ=255)
 ggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTc‑gcg                                                                                                                                 <  2:16495867/137‑1 (MQ=255)
  gcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCgg                                                                                                                                <  1:7909170/137‑1 (MQ=255)
   cAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCTTGTAGCGGCCCTTTC‑GCggg                                                                                                                               <  2:5386470/137‑1 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGGCCTTTT‑TCgggg                                                                                                                              >  1:7414691/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGGCCTTTT‑Gggggg                                                                                                                              >  1:16788541/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTc‑gcgtgc                                                                                                                              <  1:13006552/137‑4 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GTgggt                                                                                                                              >  2:15589373/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCgggg                                                                                                                              >  2:6264795/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCggga                                                                                                                              >  2:8870170/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGc                                                                                                                              <  2:7547199/137‑1 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCCTTT‑TCgggg                                                                                                                              >  1:9228284/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGACCTTTC‑GCgggg                                                                                                                              >  2:8870169/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGACCTTTC‑GCgggg                                                                                                                              >  1:6538312/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGACCCTTC‑GTgggg                                                                                                                              >  1:8452268/1‑136 (MQ=255)
     gACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCgggag                                                                                                                             >  2:11332817/1‑135 (MQ=255)
     gACCGGGGTGGAGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGTGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCCTTc‑gtgagaa                                                                                                                             >  2:7685957/1‑131 (MQ=255)
          gggTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCCTTC‑TCCGGCACGccc                                                                                                                        >  1:18391699/1‑137 (MQ=255)
           ggTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGTCCTTTT‑TCGGGGATGtccc                                                                                                                       >  1:4631937/1‑136 (MQ=255)
           ggTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑CTGGGTATTcccc                                                                                                                       >  1:9054848/1‑136 (MQ=255)
            tgggtgttgttCGGTGGGGTGTTT‑GGGGTTTGCCGGCAGGTGTGGTCGTGGGGTGCGTGGGGTGTGGGGGGGGAGGGGGTTGGGCTGGTCTGACAAACGG‑CCTCTGCTGGGAGTGGCCTTTTC‑GAGGGAGTGCCcgc                                                                                                                      <  2:17711316/132‑1 (MQ=255)
              ggCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGGCCTTTT‑TTGGGGATGCCcgcaa                                                                                                                    >  1:18347650/1‑135 (MQ=255)
              ggCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GTGGGTATGCCCGCGa                                                                                                                    >  1:5948215/1‑137 (MQ=255)
              ggCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAAAAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGCAGTTCCGCGa                                                                                                                    >  2:15153114/1‑137 (MQ=255)
                     ttCGATGAGTTGGTT‑GGGGTTTTCTGGCGGGTTTTGGCGGGGGGTGTGTGGGATGGGGGGGGGAAGCGGATTGGGTGGGTTGGACAAAAGG‑TCTCCACTTGTAGCGGCCCTTTC‑GGGGGCATGCCCGCGAAAGGGcc                                                                                                             <  2:15678484/136‑1 (MQ=255)
                     gTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑TTGGGGATGCCCCCCAAAgggac                                                                                                             >  2:2058827/1‑135 (MQ=255)
                        tgaggagtggtTGGGGGTGTGCCGGCAGTTGTTGGCGCGGCATGCGCGGGATGTGGGTGGGCAGTGGGTCGTGCTGGTCTGACCAACAT‑CTTCCGCCTGTAGCGGCCCTTTC‑GAGGGCATGCCCGCGAAGGGGCCgg                                                                                                           <  2:12988147/131‑1 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGGCCCTTT‑GCGGGAGTGTCCACGAAAagggcagg                                                                                                          >  1:7702148/1‑132 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑TCGGGTACTCCCGCGAAAAGGACggt                                                                                                          >  1:4003537/1‑137 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GTGGGGATGCCCACGAAAgggacagg                                                                                                          >  1:4003536/1‑132 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCCTTT‑TCGGGGACGCCCCCGAAAaggacagt                                                                                                          >  2:6907087/1‑132 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGACCCTTC‑TGGGGCACGCCCGAGAAAAGGACggt                                                                                                          >  2:4646571/1‑137 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCATGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCCTTT‑TTGGGCATTTCCACGAAAagggaggg                                                                                                          >  1:4420057/1‑132 (MQ=255)
                            ggttggtT‑GGGGTTTTCTGGCGGGTTTTGGTGGGGTGTGTTTGGGGTGTGGGTGGGAAGCGGATCGTGTTGTTCGGACAAACAG‑TCTCCGCTTGGAGGGGCCTTTTT‑GGGGGCGTGCCCGCGAAAGGGCCggtacag                                                                                                      <  2:7272856/134‑1 (MQ=255)
                            aGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑TCGGGTATGCCCGCGAAAGGGCCgggcaag                                                                                                      >  2:3538680/1‑132 (MQ=255)
                            aGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑TCGGGCATGCCCCCTAAAAGGCCaggacaa                                                                                                      >  2:11639162/1‑136 (MQ=255)
                            aGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGACTGCCTACGAAAGGGACggtccag                                                                                                      >  2:7315968/1‑137 (MQ=255)
                            aGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCCTTC‑TCCGGTATGCCCAATAAAGGGGCggtacag                                                                                                      >  2:8199041/1‑137 (MQ=255)
                             ggtggtT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTT‑GCGGGTATGTCCGCGAAAGGGACagtgcagg                                                                                                     >  2:2127772/1‑137 (MQ=255)
                                   t‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGTATGTCCCCGAAAAGGACGGTACAGGTGcagc                                                                                               >  2:11611428/1‑137 (MQ=255)
                                   t‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGACCTTTC‑CTAGGCATACCCGCCAAAGGGACagcaaaggtacaaa                                                                                               >  2:13315669/1‑135 (MQ=255)
                                     ggggTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGACCTTTC‑TCGGGGATACCCACGAAAAGGGCGGTGCAAGGgcagca                                                                                              >  1:9470986/1‑137 (MQ=255)
                                             ccGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGGCCTTTT‑GTTGGGATGTCCCCCAAAAGGAAGGGACAAGTACAACaaaaacccg                                                                                      >  1:12700288/1‑132 (MQ=255)
                                               ggCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCATCGGATCGTGCTGGTCTGACCAACAG‑TCTACGCCTGTAGCGGCCCTTTC‑GAAGGAACGCCCCCGAAAGGGCCTGTACAGAGACAAAAGAACATAgga                                                                                    >  2:8664336/1‑135 (MQ=255)
                                                tcAGGTGTTGTCGTGGTGTGCGGGGGGTGTGGGGGGGCAGGGGATTGTGTGGGTTTGACCAACGG‑TCTCCGTTGGGGGTGGCCTTTTG‑GGGGGAGTGCCTGAGAAGGGGCCGGTACAGGTACAGCAGAACTTAgcgc                                                                                   <  2:7208984/136‑1 (MQ=255)
                                                gaaGGTGTGGGCGTGGTGTGCGCGGAGTTTGGTTGGGAGGAGGATCGTGTTGTTTTGACCAACAT‑TCCCCCCCTGTAGCGTCCTTTTC‑GCGGGCGTGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAgcgc                                                                                   <  2:9550899/135‑1 (MQ=255)
                                                acAGGTGTTGGCGGGGCATGCGCGAGATGTGGGTGTGCAGCGGATCGTGTTGGTCTGACCAACAG‑TCTCCGCCTGGAGCGTCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAgcgc                                                                                   <  2:12505825/136‑1 (MQ=38)
                                                 tgggTTTTGGCGCGGTGTGCTCGGAATGTTGGTGTGCGGTGGGTTGTGTTGTTCTG‑CCAACTGTTCTCCGCCTGGAGCGCCCCTTTT‑GCGGGCATCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGcc                                                                                  <  2:2412852/135‑1 (MQ=255)
                                                 cAGTTGTTGGCGCGGTGTGCGTGGGGTGTGTTGGGGCGGCGGATCGTGCTGTTCTGACCAACAG‑TCTCCGCCTGTGGCGGCCCTTTC‑GCGGGCGTTCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGcc                                                                                  <  2:18139400/137‑1 (MQ=37)
                                                 cAGGGGTGGGAGTGGTGTGGGCGGGATGTGGGGGGGCGGCGGATTGTGTGGGTCTGCCCAACGG‑TCCCCGCCTGCAGCGACCCTTCC‑GCGGGCAGGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGcc                                                                                  <  2:16919973/137‑1 (MQ=255)
                                                 aaGGGGTTGGTGCGGGATGCGAGGGTGTTGGGTGGGAAGCGGGTCGTGCTGGTTTGACCAACAT‑TCTCCGCTTGTAGTGTCCTTTTC‑GCGGGCATGCCCGTGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGcc                                                                                  <  2:16919974/136‑1 (MQ=255)
                                                  aGGTGTTGTCGCGGTAGGCGCGGGGTGTGGGTGGGAAGTGGACCTTGTTGTTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTT‑GTGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCa                                                                                 <  2:14054500/137‑1 (MQ=37)
                                                     tgtgGGCGCGTGATGCGCGGGATGTGGGTGGGCAGCGGATCGGGTTGGTCTGACAAACGG‑TCTCCGCCTGGAGAGGCCTTTCC‑GTGGACATCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑ct                                                                             <  2:17046530/137‑1 (MQ=255)
                                                          tcgcgGTATGTGCGGGGTGTGGGTGGGCGGGGGGTTGTGTTGGTCGGACAAACGG‑TTTCTGCTGGGAGTGGCCTTTTT‑GGGGGCGTGTCCGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCtt                                                                        <  2:11326056/136‑1 (MQ=255)
                                                          gcgtGGTGTGCGGGGGGTGTGGGGGGGAAGGGGATTGTGCTGGTCTAACCAACGT‑CCTCCGCCTGGGGCGGCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCtt                                                                        <  2:12757529/137‑1 (MQ=25)
                                                          gcacgGTGTGCGCGGGATGTGGTGGTGCAGCGGATCTTGCGGGCCTGACCAACAT‑CCTCCTCCTGTAGCGTCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCtt                                                                        <  2:10028497/134‑1 (MQ=37)
                                                                tATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGGCCTTTT‑TTGGGTATGGCCTCGAAAAGGGCCGTGAAGGTCAAACAAAACCTCGCGCCCA‑CTTTCTTT‑tacca                                                                 >  2:3733980/1‑137 (MQ=255)
                                                                     gcgGGATGTGGTTGGGCAGCAGATCGTGCTGGTCCGACAAAAAG‑TCTCCGCCTGTGGCGTCCCTTCC‑GAGGGCGGTCACAAAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTg                                                            <  2:6152335/137‑1 (MQ=255)
                                                                       gggATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑TTTGGACTTCCCACGAAAGGGTCGGTACAGGTTCAACAGGACTTCGCGTCAG‑CTTTCTTT‑CCCCCCCCtctg                                                          >  2:17088336/1‑134 (MQ=255)
                                                                       gggATGTGGGGGGGCAGCGAATCGTGTTGGCCGGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GTGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTgcg                                                          <  2:2789586/137‑1 (MQ=38)
                                                                        gggtgtgGTGGGGCAGCGGGTTGTGTTGGTTTGACAAAAGT‑TTTCTTCTTGTAGTGTCCCTTTC‑GCGGGCATTCCCGCAAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTgcgc                                                         <  2:8064145/134‑1 (MQ=255)
                                                                        gggtgtgGGGGGGCGGTGGGTTGTGTTGTTTTGACAAACGG‑TCTCTGCTTGGAGTGTCCTTTTC‑GTGGGCGTACCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTgcgc                                                         <  2:8321820/134‑1 (MQ=255)
                                                                         gATTTGGGTGGCTAGCGGATTGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCTTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGcgcc                                                        <  2:17926973/137‑1 (MQ=39)
                                                                             tgggtgggCAGGGGATTGTGCTGTCTTGACCAACGT‑TCTCCCCCTGTAGCGGCCCTTTC‑GCGGGCATCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGccgccc                                                    <  2:9579190/137‑1 (MQ=255)
                                                                             tgggtgggCAGGGGATTGGGCTGGTCTGACAAACAT‑TTTCCGCTGGGAGTGTCCTTTCC‑GTGGGAGTGCCCGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGccgccc                                                    <  2:7984115/137‑1 (MQ=255)
                                                                             ggggggggAAGGGGATGGTGTTGTTCTAACAAACGT‑CCTCCCTCTGGATCGTCCTTTTC‑GTGGGCATCCCCGAAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGccgccc                                                    <  1:16171733/136‑1 (MQ=255)
                                                                                   ggaggCGGGTTGTGTTGGTCTAACAAACAT‑TCTCCTCTGGTACCGTCCTTTCC‑GCGGGAGTTCCCGAGAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAg                                              <  1:8293544/133‑1 (MQ=255)
                                                                                      agtggATTGTGTTGTTCTGCCCAACGT‑TCTCCGCCTGTGGC‑GCCCTTTCTGGGGGCAGGCCCGCAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGAt                                           <  2:697176/134‑1 (MQ=255)
                                                                                      aacGGATCGTGCTGGTTGGACAAACAG‑CCTCCACTTGGAGCGTCCCTTTC‑GCGGACCGTCCCGCGAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGAt                                           <  1:7982442/135‑1 (MQ=255)
                                                                                      aGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑TCGGGCACGCCCGCAAAAGGGCTAGTACAAGTAAAACAGAACTTCGCGCCAG‑CTCTCTTT‑CCCCACCCCGAGCCACCCACCCAAGAt                                           >  2:16392401/1‑137 (MQ=255)
                                                                                      aGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGGAAGTCCGCGAAAAGGACGGTGCAAGTACCACAAAACTTCTCACCAGACTCCTTTT‑CCCCACCCT‑CGCCCCCCGCCCaaagg                                           >  2:13230040/1‑133 (MQ=255)
                                                                                       atggATCGTGCTGGTCTAACAAACAT‑CCTCCGCCGGAAGCGTCCTTTTC‑TCGGGCCTTCCCGAGAAAGGCCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATc                                          <  1:15447963/135‑1 (MQ=255)
                                                                                        cGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGACCCTTC‑TCGGGGATGCCCACCAAAGGGCAAGTACAAGTGCAGCAAAACCTCACACCCA‑ATCTCTTT‑CCCCCCCCCCCGCCCCCCACCCCAGATca                                         >  2:18912979/1‑137 (MQ=255)
                                                                                           aTCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑CCGGGGATGCCCCCGAAAAGGACAGTACAGGTGCAAAAAAAATTCGCGCCAA‑CTCTCTTTCCCCCACCCTCCCCCCCCCCCCCAggtccacc                                       >  2:12756389/1‑131 (MQ=255)
                                                                                           aTCGTGCTGGTCCGACCAACAG‑TCTCCGCCTGTAGCGGACCCTTT‑TCGGGGACGCCCGCGAAAGGGACGGTACAGGTACAACAAAAATTCGCAACCG‑CTCTCTTT‑TACCCCCCCTCGCCCCCCACCCCGGATaagcc                                      >  2:18116488/1‑137 (MQ=255)
                                                                                             tgTGCTGTTCGGACAAACGT‑TCTCCTCTTGTGGCGTCCTTTTT‑GGGGACGTCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATcagccag                                    <  2:9538402/136‑1 (MQ=255)
                                                                                             cgggttGGTCTAACAAACGG‑TCCCCGCCTGTAGCGTCCCTTTC‑GCGGACGTCCCCGAGAAAGGTCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATcagccag                                    <  1:17660136/132‑1 (MQ=255)
                                                                                              tttcTGTTCTAACAAACGT‑TCCCCGCCTGGAGCGTCCCTTTC‑GCGGCCATCCCCGCAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATcagccagc                                   <  2:10914875/134‑1 (MQ=255)
                                                                                              gtgtTGTTCGGACAAACGT‑TCTCCGCTTGGAGCGTCCCTTTC‑GGGGGCGTCCCCGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATcagccagc                                   <  2:14873964/137‑1 (MQ=255)
                                                                                              gTGCGGGTCGAACCAACAG‑TCTCCCCCTGTAGCGCCCCTTTC‑GCGGACATACCCGCAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATcagccagc                                   <  2:5683388/137‑1 (MQ=255)
                                                                                                 ctgttttGACAAACAG‑TCTCCGCTTGGAGTGCCCCTTTC‑GTGGGCATCCCCTCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCaa                                <  1:4981478/131‑1 (MQ=255)
                                                                                                  tGGTTTAACAAAAAG‑TCTTCGTTTGTGGCGGCCTTTTT‑GCGGACATTCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAAt                               <  2:12207537/137‑1 (MQ=255)
                                                                                                  tGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑TTGGGAATTCCCCCTAAAAGGCCGGGTCCGGGACCGCCAAACTTCTAACCAG‑CTCCCTTT‑CATCACCCCGCACCGCCCCCCCCCAGTCAGCCAACCAAt                               >  2:5632317/1‑137 (MQ=255)
                                                                                                  tGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGCAGGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAAt                               <  2:17292622/137‑1 (MQ=255)
                                                                                                  tGGTCGGACAAACGG‑TTTCTGCTTGTGGCGGCCTTTTT‑TTGGGCGTTCCCGCGAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAAt                               <  2:6021830/137‑1 (MQ=255)
                                                                                                  tGGTCGGACAAACGG‑TCTCCGCTGGGGTCGTCCTTTTC‑GGGGACGTGCCGGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAAt                               <  2:13990715/137‑1 (MQ=255)
                                                                                                       tGACCAACAG‑TCTCCGCCTGTAGCGGCCCCTTT‑TTGGGTAGGCCCACGAAAGGGACAGGAAAAGGACAGAAGAAATTCACACCCA‑CGCTCCTT‑CCCCCACCCGCGCCGCCCCCCCAAAAGCAGCCAGCCAATccccc                          >  2:11237143/1‑136 (MQ=255)
                                                                                                        gACAAAAAG‑TCTCCCCCGGGAGGGGCCCTTTA‑TTGGAAATCCCCGGGAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAg                         <  2:10015803/137‑1 (MQ=255)
                                                                                                         aCCAACGG‑TCTCCGCTTGTAGTGTCCTTTTC‑GTGGTCGTCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGc                        <  1:17424598/137‑1 (MQ=255)
                                                                                                         aCCAACAG‑TCTCCGTTTGGAGCGGCCCTTCC‑GTGGGCGTGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGc                        <  2:1489413/137‑1 (MQ=255)
                                                                                                         aCCAAAGG‑TCTCCGCTTGGGGTGGCCTTTTC‑GTGGGCGTGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGc                        <  2:18685715/137‑1 (MQ=255)
                                                                                                         aCCAAAAG‑TCTCCGCTTGTAGCGGCCCTTTC‑TCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGc                        <  2:4073736/137‑1 (MQ=255)
                                                                                                                    tcCGCCTTGAGCGTCCCTTTC‑GCGGACGTCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCttt              <  2:17526091/137‑1 (MQ=255)
                                                                                                                    tcCGCCTGTAGCGGCCCTTTC‑TCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCttt              <  2:12531932/137‑1 (MQ=255)
                                                                                                                              gcgcCCCTTTT‑GCGGCCATTCCCCCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGTGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:9779761/137‑1 (MQ=255)
                                                                                                                              gCGTCCTTTTC‑TCGGTCGTCCCAGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:10949161/137‑1 (MQ=255)
                                                                                                                              gCGTCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:15996975/137‑1 (MQ=255)
                                                                                                                                ggCCTTTTC‑GCGTGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAgg  <  2:18694225/137‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                 
TGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCTGTAGCGGCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAG‑CTCTCTTT‑CACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGG  >  AE015451_phaM1/5199521‑5199783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑