Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,199,6570CT29.9% 94.8 / 28.1 132intergenic (+32/+41)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–
Reads supporting (aligned to +/- strand):  ref base C (40/42);  new base T (17/18);  total (59/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 139 total aligned reads displayed.

TGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGG  >  AE015451_phaM1/5199521‑5199783
                                                                                                                                            |                                                                                                                              
tggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTc‑gc                                                                                                                                <  2:15779540/137‑1 (MQ=255)
tggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTc‑gc                                                                                                                                <  2:9858590/137‑1 (MQ=255)
tggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTc‑gc                                                                                                                                <  1:17682806/137‑1 (MQ=255)
 ggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTc‑gcg                                                                                                                               <  2:8047672/137‑1 (MQ=255)
 ggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTc‑gcg                                                                                                                               <  2:16495867/137‑1 (MQ=255)
  gcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCgg                                                                                                                              <  1:7909170/137‑1 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGGCCTTTT‑TCgggg                                                                                                                            >  1:7414691/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGGCCTTTT‑Gggggg                                                                                                                            >  1:16788541/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTc‑gcgtgc                                                                                                                            <  1:13006552/137‑4 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTT‑TCgggg                                                                                                                            >  2:14468366/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCggga                                                                                                                            >  2:8870170/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGc                                                                                                                            <  2:7547158/137‑1 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGc                                                                                                                            <  2:7547199/137‑1 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGc                                                                                                                            <  1:17211095/137‑1 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCCTTT‑TCgggg                                                                                                                            >  1:9228284/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGACCTTTC‑GCgggg                                                                                                                            >  1:6538312/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGACCTTTC‑GCgggg                                                                                                                            >  2:8870169/1‑136 (MQ=255)
    aGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGACCCTTC‑GTgggg                                                                                                                            >  1:8452268/1‑136 (MQ=255)
     gACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCgggag                                                                                                                           >  2:11332817/1‑135 (MQ=255)
      aCCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGCAt                                                                                                                          <  2:17905947/137‑1 (MQ=255)
       ctggggTGGTGTTGTTCGATGAGGTGGTG‑GGGGTTTGCCGGCAGGTGTTGGCGTGGTATGGTCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTTTAACCAACAG‑TCTCCTCCT‑GCATCGTCCCTTTC‑GCGGGcgtt                                                                                                                         <  2:11232194/135‑4 (MQ=255)
          gggTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCCTTC‑TCCGGCACGccc                                                                                                                      >  1:18391699/1‑137 (MQ=255)
           ggTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGTCCTTTT‑TCGGGGATGtccc                                                                                                                     >  1:4631937/1‑136 (MQ=255)
            tgggtgttgttCGGTGGGGTGTTT‑GGGGTTTGCCGGCAGGTGTGGTCGTGGGGTGCGTGGGGTGTGGGGGGGGAGGGGGTTGGGCTGGTCTGACAAACGG‑CCTCTGCTG‑GGAGTGGCCTTTTC‑GAGGGAGTGCCcgc                                                                                                                    <  2:17711316/132‑1 (MQ=255)
              ggCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGGCCTTTT‑TTGGGGATGCCcgcaa                                                                                                                  >  1:18347650/1‑135 (MQ=255)
              ggCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAAAAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGCAGTTCCGCGa                                                                                                                  >  2:15153114/1‑137 (MQ=255)
               gCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGTGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTT‑TCTGGCACGcccccaaa                                                                                                                 >  2:673505/1‑132 (MQ=255)
                   tGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTT‑TCGGGCATGCCCCCGAAAagg                                                                                                             >  2:4414087/1‑137 (MQ=255)
                     ttCGATGAGTTGGTT‑GGGGTTTTCTGGCGGGTTTTGGCGGGGGGTGTGTGGGATGGGGGGGGGAAGCGGATTGGGTGGGTTGGACAAAAGG‑TCTCCACTT‑GTAGCGGCCCTTTC‑GGGGGCATGCCCGCGAAAGGGcc                                                                                                           <  2:15678484/136‑1 (MQ=255)
                        tgaggagtggtTGGGGGTGTGCCGGCAGTTGTTGGCGCGGCATGCGCGGGATGTGGGTGGGCAGTGGGTCGTGCTGGTCTGACCAACAT‑CTTCCGCCT‑GTAGCGGCCCTTTC‑GAGGGCATGCCCGCGAAGGGGCCgg                                                                                                         <  2:12988147/131‑1 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGGCCCTTT‑GCGGGAGTGTCCACGAAAagggcagg                                                                                                        >  1:7702148/1‑132 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑TCGGGTACTCCCGCGAAAAGGACggt                                                                                                        >  1:4003537/1‑137 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCCTTT‑TCGGGGACGCCCCCGAAAaggacagt                                                                                                        >  2:6907087/1‑132 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGACCCTTT‑TTGGGGAGGTCCTCGAAAggggcagg                                                                                                        >  1:5024254/1‑132 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGACCCTTC‑TGGGGCACGCCCGAGAAAAGGACggt                                                                                                        >  2:4646571/1‑137 (MQ=255)
                        gATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCATGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCCTTT‑TTGGGCATTTCCACGAAAagggaggg                                                                                                        >  1:4420057/1‑132 (MQ=255)
                            ggttggtT‑GGGGTTTTCTGGCGGGTTTTGGTGGGGTGTGTTTGGGGTGTGGGTGGGAAGCGGATCGTGTTGTTCGGACAAACAG‑TCTCCGCTT‑GGAGGGGCCTTTTT‑GGGGGCGTGCCCGCGAAAGGGCCggtacag                                                                                                    <  2:7272856/134‑1 (MQ=255)
                            aGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑TCGGGTATGCCCGCGAAAGGGCCgggcaag                                                                                                    >  2:3538680/1‑132 (MQ=255)
                            aGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑TCGGGCATGCCCCCTAAAAGGCCaggacaa                                                                                                    >  2:11639162/1‑136 (MQ=255)
                            aGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCCTTC‑TCCGGTATGCCCAATAAAGGGGCggtacag                                                                                                    >  2:8199041/1‑137 (MQ=255)
                             ggtggtT‑GGGGTTTGCCGGCAGGTGTTGGCGTGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGAATGCCCCCAAAAAGGACggtacagg                                                                                                   >  2:18909620/1‑137 (MQ=255)
                             ggtggtT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTT‑TTGGGCATGCCCACGAAAGGGGCagtaaaag                                                                                                   >  2:549791/1‑133 (MQ=255)
                             ggtggtT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTT‑GCGGGTATGTCCGCGAAAGGGACagtgcagg                                                                                                   >  2:2127772/1‑137 (MQ=255)
                             ggtggtT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCCTTC‑TCGGGAATGCCCACGAAAAGGACggtacagg                                                                                                   >  2:13570667/1‑137 (MQ=255)
                                   t‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGACCTTTC‑CTAGGCATACCCGCCAAAGGGACagcaaaggtacaaa                                                                                             >  2:13315669/1‑135 (MQ=255)
                                     ggggTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGGATGTCCTCGGAAGGGGCGgtgcaaggacagaa                                                                                            >  1:9470985/1‑135 (MQ=255)
                                             ccGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGGCCTTTT‑GTTGGGATGTCCCCCAAAAGGAAGGGACAAGTACAACaaaaacccg                                                                                    >  1:12700288/1‑132 (MQ=255)
                                               ggCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCATCGGATCGTGCTGGTCTGACCAACAG‑TCTACGCCT‑GTAGCGGCCCTTTC‑GAAGGAACGCCCCCGAAAGGGCCTGTACAGAGACAAAAGAACATAgga                                                                                  >  2:8664336/1‑135 (MQ=255)
                                                gaaGGTGTTGGCGTGGTGTGAGTGGGATGTGGGTGGGAAGTGGGTTGTGTTGTTCGAACCAAGGG‑TTTCCGCTT‑GGGGTGTCCTTTTT‑GTGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAgcgc                                                                                 <  2:7209029/135‑1 (MQ=255)
                                                gaaGGTGTGGGCGTGGTGTGCGCGGAGTTTGGTTGGGAGGAGGATCGTGTTGTTTTGACCAACAT‑TCCCCCCCT‑GTAGCGTCCTTTTC‑GCGGGCGTGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAgcgc                                                                                 <  2:9550899/135‑1 (MQ=255)
                                                acAGGTGTTGGCGGGGCATGCGCGAGATGTGGGTGTGCAGCGGATCGTGTTGGTCTGACCAACAG‑TCTCCGCCT‑GGAGCGTCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAgcgc                                                                                 <  2:12505825/136‑1 (MQ=38)
                                                 tgggTTTTGGCGCGGTGTGCTCGGAATGTTGGTGTGCGGTGGGTTGTGTTGTTCTG‑CCAACTGTTCTCCGCCT‑GGAGCGCCCCTTTT‑GCGGGCATCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGcc                                                                                <  2:2412852/135‑1 (MQ=255)
                                                 cAGTTGTTGGCGCGGTGTGCGTGGGGTGTGTTGGGGCGGCGGATCGTGCTGTTCTGACCAACAG‑TCTCCGCCT‑GTGGCGGCCCTTTC‑GCGGGCGTTCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGcc                                                                                <  2:18139400/137‑1 (MQ=37)
                                                 aaGGGGTTGGTGCGGGATGCGAGGGTGTTGGGTGGGAAGCGGGTCGTGCTGGTTTGACCAACAT‑TCTCCGCTT‑GTAGTGTCCTTTTC‑GCGGGCATGCCCGTGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGcc                                                                                <  2:16919974/136‑1 (MQ=255)
                                                  aGGTGTTGTCGCGGTAGGCGCGGGGTGTGGGTGGGAAGTGGACCTTGTTGTTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTT‑GTGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCa                                                                               <  2:14054500/137‑1 (MQ=37)
                                                   gtttttGGCGGGGTCTGCGTGGGGTGTGGGTGGGCAGTGGCTTGTGTTGGTTTGACCAAAGT‑TTTCCGCTT‑GGGGTGGCCTTTTT‑GGGGACGTCCCTGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAg                                                                              <  2:11709011/133‑1 (MQ=255)
                                                    gtgtTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCTGGCACGCCCCCGAAAGGGCCCGGGCAAGTACAACAGAACCTCGCACCAGc                                                                             >  2:12838677/1‑137 (MQ=255)
                                                     tgtgGGCGCGTGATGCGCGGGATGTGGGTGGGCAGCGGATCGGGTTGGTCTGACAAACGG‑TCTCCGCCT‑GGAGAGGCCTTTCC‑GTGGACATCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGct                                                                            <  2:17046530/137‑1 (MQ=255)
                                                          tcgcgGTATGTGCGGGGTGTGGGTGGGCGGGGGGTTGTGTTGGTCGGACAAACGG‑TTTCTGCTG‑GGAGTGGCCTTTTT‑GGGGGCGTGTCCGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCtt                                                                       <  2:11326056/136‑1 (MQ=255)
                                                          gcgtGGTGTGCGGGGGGTGTGGGGGGGAAGGGGATTGTGCTGGTCTAACCAACGT‑CCTCCGCCT‑GGGGCGGCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCtt                                                                       <  2:12757529/137‑1 (MQ=25)
                                                          gcacgGTGTGCGCGGGATGTGGTGGTGCAGCGGATCTTGCGGGCCTGACCAACAT‑CCTCCTCCT‑GTAGCGTCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCtt                                                                       <  2:10028497/134‑1 (MQ=37)
                                                                     gcgGGATGTGGTTGGGCAGCAGATCGTGCTGGTCCGACAAAAAG‑TCTCCGCCT‑GTGGCGTCCCTTCC‑GAGGGCGGTCACAAAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTg                                                            <  2:6152335/137‑1 (MQ=255)
                                                                        ggttttggttgggAAGCGGGTCGTTCGGGTCTGAAC‑ACCT‑TTTCCACTTGGTGGCGCCCCTTTG‑GTGCGCATACCTGGGAAAGGCCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTgcgc                                                         <  1:3854800/132‑1 (MQ=255)
                                                                        gggtgtgGTGGGGCAGCGGGTTGTGTTGGTTTGACAAAAGT‑TTTCTTCTT‑GTAGTGTCCCTTTC‑GCGGGCATTCCCGCAAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTgcgc                                                         <  2:8064145/134‑1 (MQ=255)
                                                                        gggtgtgGGGGGGCGGTGGGTTGTGTTGTTTTGACAAACGG‑TCTCTGCTT‑GGAGTGTCCTTTTC‑GTGGGCGTACCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTgcgc                                                         <  2:8321820/134‑1 (MQ=255)
                                                                         gATTTGGGTGGCTAGCGGATTGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCTTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGcgcc                                                        <  2:17926973/137‑1 (MQ=39)
                                                                             tgggtgggCAGGGGATTGTGCTGTCTTGACCAACGT‑TCTCCCCCT‑GTAGCGGCCCTTTC‑GCGGGCATCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGccgccc                                                    <  2:9579190/137‑1 (MQ=255)
                                                                             tgggtgggCAGGGGATTGGGCTGGTCTGACAAACAT‑TTTCCGCTG‑GGAGTGTCCTTTCC‑GTGGGAGTGCCCGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGccgccc                                                    <  2:7984115/137‑1 (MQ=255)
                                                                             tgggtgggCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GGAGCGGCCCCTTC‑TCAGGGCTTCCCACGAAAGGGCCCGGAAAAGTAAAACAGAAACTAGAGCCCGATCCGTTTCACCCACCCGCAccgccc                                                    >  2:10514484/1‑137 (MQ=255)
                                                                             ggggggggAAGGGGATGGTGTTGTTCTAACAAACGT‑CCTCCCTCT‑GGATCGTCCTTTTC‑GTGGGCATCCCCGAAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGccgccc                                                    <  1:16171733/136‑1 (MQ=255)
                                                                                  gggCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCCTTC‑CCGGGCCCTCCCCCGAAAAGGTCGGGCCAAGGAAAACAGAAATTCGCACCAACTCTCTTTCACCACCCCGCCCCCCCCGCCCa                                               >  2:2293831/1‑137 (MQ=255)
                                                                                   ggaggCGGGTTGTGTTGGTCTAACAAACAT‑TCTCCTCTG‑GTACCGTCCTTTCC‑GCGGGAGTTCCCGAGAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAg                                              <  1:8293544/133‑1 (MQ=255)
                                                                                   ggCAGCGGATTGTGTTGGTCTGACCACCAG‑TCCCCGCCT‑GTAGCGTCCTTTTC‑TCGTGCATGCCCGCGAAAGGTCCGGTACAGGTACAGCAGAACTTAGCGCCAGTTCTCTTTCACCACCCTGCGCCGCCCGCCCAg                                              <  2:15189674/137‑1 (MQ=255)
                                                                                      agtggATTGTGTTGTTCTGCCCAACGT‑TCTCCGCCT‑GTGGC‑GCCCTTTCTGGGGGCAGGCCCGCAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGAt                                           <  2:697176/134‑1 (MQ=255)
                                                                                      aggggATTGTGTTGGTTTGACAAACAG‑TCCCCTCCT‑GTATCGTCCCTTTC‑GCGGGCATCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGAt                                           <  2:6050594/134‑1 (MQ=255)
                                                                                      aagagATCGTTCGGTTCTAACCACCAT‑TCCCCACCT‑GTCGCGCCCCTTTC‑CCTGCCGTGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCTAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGAt                                           <  1:7982441/133‑1 (MQ=255)
                                                                                       atggATCGTGCTGGTCTAACAAACAT‑CCTCCGCCG‑GAAGCGTCCTTTTC‑TCGGGCCTTCCCGAGAAAGGCCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATc                                          <  1:15447963/135‑1 (MQ=255)
                                                                                        ggggttgtgtTGGTCCGACAAACAT‑TCTCCGCTT‑GGGGTGTCCTTTTC‑GTGGGCATACCCGAAAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATca                                         <  1:8678585/131‑1 (MQ=255)
                                                                                        cGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGACCCTTC‑TCGGGGATGCCCACCAAAGGGCAAGTACAAGTGCAGCAAAACCTCACACCCAATCTCTTTCCCCCCCCCCCGCCCCCCACCCCAGATca                                         >  2:18912979/1‑137 (MQ=255)
                                                                                          gATCGTGCTGGTCGGACCAACAT‑TCTCCGCTT‑GTAGTGGCCCTTGC‑GTGGGCATGCCTGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcagc                                       <  2:12567859/137‑1 (MQ=255)
                                                                                           gtCGTGCTGGTCTGACCAACAG‑CCTCCGCCG‑TGAGCGGCCCTTTC‑CCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcagcc                                      <  2:16858832/136‑1 (MQ=39)
                                                                                             tgTGCTGTTCGGACAAACGT‑TCTCCTCTT‑GTGGCGTCCTTTTT‑GGGGACGTCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcagccag                                    <  2:9538402/136‑1 (MQ=255)
                                                                                             cgggttGGTCTAACAAACGG‑TCCCCGCCT‑GTAGCGTCCCTTTC‑GCGGACGTCCCCGAGAAAGGTCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcagccag                                    <  1:17660136/132‑1 (MQ=255)
                                                                                              gtgtTGTTCGGACAAACGT‑TCTCCGCTT‑GGAGCGTCCCTTTC‑GGGGGCGTCCCCGAGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcagccagc                                   <  2:14873964/137‑1 (MQ=255)
                                                                                                 ctgttttGACAAACAG‑TCTCCGCTT‑GGAGTGCCCCTTTC‑GTGGGCATCCCCTCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCaa                                <  1:4981478/131‑1 (MQ=255)
                                                                                                  tGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑TTGGGAATTCCCCCTAAAAGGCCGGGTCCGGGACCGCCAAACTTCTAACCAGCTCCCTTTCATCACCCCGCACCGCCCCCCCCCAGTCAGCCAACCAAt                               >  2:5632317/1‑137 (MQ=255)
                                                                                                  tGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGCAGGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAAt                               <  2:17292622/137‑1 (MQ=255)
                                                                                                  tGGTCGGACAAACGG‑TTTCTGCTT‑GTGGCGGCCTTTTT‑TTGGGCGTTCCCGCGAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAAt                               <  2:6021830/137‑1 (MQ=255)
                                                                                                        gACAAAAAG‑TCTCCCCCG‑GGAGGGGCCCTTTA‑TTGGAAATCCCCGGGAAGGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAg                         <  2:10015803/137‑1 (MQ=255)
                                                                                                         aCCAAAGG‑TCTCCGCTT‑GGGGTGGCCTTTTC‑GTGGGCGTGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGc                        <  2:18685715/137‑1 (MQ=255)
                                                                                                         aCCAAAAG‑TCTCCGCTT‑GTAGCGGCCCTTTC‑TCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGc                        <  2:4073736/137‑1 (MQ=255)
                                                                                                                    tcagCCT‑GTAGTGGTCTTTTC‑GAGGGCCTGCCCGCGAAGGGTCCTGTATAGGTGCAGCAGAGCTTAGCACCAGCTTTCTTTCACTACCCTTCGCCGCCCGCCCAGGATCAGCTAGCCAATCCCCGGCAACAGGCttt              <  2:18337978/134‑1 (MQ=255)
                                                                                                                    tcCGCCT‑GTAGCGGCCCTTTC‑TCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCttt              <  2:12531932/137‑1 (MQ=255)
                                                                                                                          ctggatcGGCCCTTTC‑TCGGGAGTCCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGAcc         <  2:16938998/131‑1 (MQ=255)
                                                                                                                               gcgtctcTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:11484202/131‑1 (MQ=255)
                                                                                                                               gcgcCCCTTTT‑GCGGCCATTCCCCCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGTGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:9779761/137‑1 (MQ=255)
                                                                                                                               gcgcCCCTTTC‑GCGGGAACCCCCGAAAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGTGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:299086/137‑1 (MQ=255)
                                                                                                                               gCGTCCTTTTC‑TCGGTCGTCCCAGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:10949161/137‑1 (MQ=255)
                                                                                                                               gCGTCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:15996975/137‑1 (MQ=255)
                                                                                                                               gCGGCCCTTTT‑GCGGGCATTCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGTGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa    <  2:17420829/137‑1 (MQ=255)
                                                                                                                                            |                                                                                                                              
TGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTT‑GGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAG‑TCTCCGCCT‑GTAGCGGCCCTTTC‑GCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGG  >  AE015451_phaM1/5199521‑5199783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑