BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	5,344,486	0	T	G	27.8%	434.6 / 63.7	287	intergenic (+61/‑117)	pgi‑II CDS (glucose‑6‑phosphate isomerase)/acsA‑II CDS (acetyl‑CoA synthetase)	–/–
Reads supporting (aligned to +/- strand): ref base T (106/99); new base G (1/78); total (110/177)
Fisher's exact test for biased strand distribution p-value = 9.42e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 288 total aligned reads displayed.

AGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAA  >  AE015451_phaM1/5344351‑5344619

aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTa                                                                                                                                      <  2:13242332/137‑1 (MQ=255)

aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTa                                                                                                                                      <  1:12089378/137‑1 (MQ=255)

aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTa                                                                                                                                      <  1:13209151/137‑1 (MQ=255)

aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTa                                                                                                                                      <  2:2813534/137‑1 (MQ=255)

aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTa                                                                                                                                      <  1:4099851/137‑1 (MQ=255)

aGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGCAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTa                                                                                                                                      <  2:15756950/137‑1 (MQ=255)

 gCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTAg                                                                                                                                     <  1:15248006/137‑1 (MQ=255)

 gCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAAACCACCT‑CCCCAG‑CAAAGCGTTACCCCGTAg                                                                                                                                     >  2:11409462/1‑137 (MQ=37)

       aaGACAGCGCCGAAGATGGTTCCACCCAGGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTAGGAGCgg                                                                                                                               <  2:1244145/137‑1 (MQ=255)

       aaGACAGCGCCGAAGACGGTTCCACCCAGGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTAGGAGCgg                                                                                                                               <  2:10440089/137‑1 (MQ=255)

       aaGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGTCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTAGGAGCgg                                                                                                                               <  2:7529126/137‑1 (MQ=255)

       aaGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTAGGAGCgg                                                                                                                               <  2:10973171/137‑1 (MQ=255)

               gcCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTGAAACCACCC‑CCCAAG‑CAAAACGTTGCCCCGTCGGAGAGGGTTTcccc                                                                                                                       >  2:9942017/1‑137 (MQ=37)

                ccGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCACCT‑CCCAAG‑AAACAAGTTACCTCATAGGAGGGGGGTTccccc                                                                                                                      >  2:9260992/1‑136 (MQ=37)

                   aaGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTTGCGGGTAAAACCGCCC‑CCCAAG‑CAACGCGTTCCCTCGTAGGAGGGGGTTTTCCCACGa                                                                                                                   >  2:13160029/1‑137 (MQ=255)

                    aGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCACCT‑ACCCAG‑CAACACGTTACCTCGTCGGAGCGGGTTTACCCCCGaa                                                                                                                  >  2:10640526/1‑137 (MQ=255)

                          ttCCACCCACGCCCTGTCCACCTATTTCCGCGGCCTTCACCTCGGTGGATCCAAGCTTGTGCCGTCCTCTTCGAGGGGAAACCCACCT‑ACCAGG‑CAACGCGTTACCTCGTGGGATTGGGTTTACCCGCGAAGAggcc                                                                                                            <  2:9411908/137‑1 (MQ=255)

                            ccACCCAGGGCCGAACAAACTCCTTCCTCTGCCGTCACTGCGTTTGATCTGATTTTTTTCCGGCCTTTTCGGGGGGAAACCCTCCT‑ACCAGG‑CAACGCGTTCCCTCGGAGGAGTGGTTTCACCCGCGAAGAggccgg                                                                                                          <  2:6928505/137‑1 (MQ=255)

                            ccACCCAGGACCGAACCAACAACTTCCGCGTCCGCCACCGCGGTGAACCAGAGCTTGTGCCGGCCTCGTCGCGGGGAAACCCACCC‑ACCAGG‑AAACGCGTTACCCCGGAGGAGAGGTTTTACCCGCGAAGAggccgg                                                                                                          <  2:7648765/137‑1 (MQ=255)

                            ccACCCAAGGCCTGACCAACTCCTTCCGTGGCCGTCACCGTGTTGGATCCGAGTTTGTGCCGTCCTCTTTGTGGGTAACCCCCCCT‑ACCAGG‑CACCGTGTTACCTTGGAGGAGCGGGTTTACCCGCGAGGAggccgg                                                                                                          <  2:5838891/137‑1 (MQ=255)

                                ccAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTGAAACCACCC‑ACCCGG‑CAAAAAATTTCCCCGTCGGAGGAGGGTTCCCCACGAAAAGGACGGCCCa                                                                                                      >  2:1232328/1‑137 (MQ=255)

                                ccAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCCTCGCGGGTAAACCCACCT‑CCCAGG‑CCACGCGTTCCCTCGTAGGAGCGGGTTTCCCCGCGAAGAGGCCGGCCCa                                                                                                      >  2:18060421/1‑137 (MQ=38)

                                  aaGGCCTAATAAACTACTCCTGCGACCGTAACCGTGTTGGATCCAGGTTTGTGCGGTCTTTTTTGCGGGGAAACCCGCCG‑ACCGGG‑AAATGTGTTACTTCGGGGGAGTGGGTTCACCCGCGAAGAGGCCGGCCCAgg                                                                                                    <  2:2614291/137‑1 (MQ=255)

                                   aGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTTTCGGGTAAACCCGCCT‑ACCAGG‑CAAAACGTTACCCCGTAGGGGCGGGTTTACCCACGAAGAGGCCGGACCAggg                                                                                                   >  2:1629571/1‑136 (MQ=255)

                                     gcatGATCAACTACTTCCGCGGCCGTCACCGCGGTTGCTCCGAGCTTGTGCCGGCCTCTTCCCCAGGAAACCCCCCTCACCA‑G‑AAAAACGTTTCCTCCCAAGAAAGGGTTTACCCACGAAGAGGCCAGCCCAAGtcg                                                                                                 >  2:3529158/4‑135 (MQ=255)

                                           tCAACTACTTCCTCGGCCGTCACTGCGGTTGATCAGAGCTTGTGCTGTCCTCTTCGCGGGGAAACCCGCCT‑ACCAGG‑CAACGCTTTACCTTGTGGGAGTGGGTTGACCCGCGAAGAGGCCGGCCCAGGTTGAACAcc                                                                                           <  2:14178123/137‑1 (MQ=255)

                                           tCAACTACTTCCTCGGCCGCCACCGCGGTTGATCCAAGCTTGTGCTGGCCTCTTCGCGGGGAAACCCGCCT‑CCCAGG‑AAGCGCGTTACCTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACAcc                                                                                           <  2:13490939/137‑1 (MQ=37)

                                           tCAACGACTTCCGCGACCGCAACAGCGTTTGATCCGATCTTGTGCTGGCCTCTTCGTGGGTAACCCCGCCT‑ACCGGA‑CAACGTGTTACCTCGTAGGAGCGGTTTTACCCGCGAAGGGGCCGGCCCAGGTTGAACAcc                                                                                           <  2:6435364/137‑1 (MQ=255)

                                           ccAACTACTTCCGCGGCCGTCACCGCGGTTGACCAGAGTTTGTGCTGGCCTTTTCGCGGGGAAACCCGCCG‑ACCGGG‑CAACGCGTTACCTCGTGGGAGTGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACAcc                                                                                           <  2:13920719/136‑1 (MQ=255)

                                           caaccgactTCCGTGACCGTCACCGTGGTTGATCCGAGTTTGTTCTGTCTTCTTAGGGGGGAAACCCGCCG‑ACAGGG‑AAATGTGTTACTTTGGGGGAGTGGTTTTCCCCGGGAAGAGGCCGGCCCAGGTTGAACAcc                                                                                           <  2:5612230/131‑1 (MQ=255)

                                             aaCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCTCGGGTGAAACCACCC‑CCCCAG‑CAACACGTTGCCTCCTCGGAGGGGGTTTTCCCCCGAAAAGGCCAGACCAAATTTAACAcccc                                                                                         >  1:18880870/1‑135 (MQ=255)

                                                tactTCTGGGGCAGCCACCGGGGTGGATCAGAGTTTGTTCCGTCCTTTTCGGGGGGAAACCCGCCT‑ACCGGG‑CAACGTGTTACTTTGGGGGAGCGGGTTCACCAGAGAAGGGGCCGGCCCAGGTTGAACACCGCTcc                                                                                      <  1:18311009/137‑1 (MQ=255)

                                                tactTCCGTGGCAGCCACCGTGGTGGATCAGAGCTTGTGCTGGCCTCTTCGCGGGGAAACCCGCCC‑ACCAGG‑AAACGTGTTACCTTGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTcc                                                                                      <  2:7938208/137‑1 (MQ=25)

                                                gatttCGGCGGCCGCAACCGGGGTGGATCCGGGTTTGTGCGGGCCTTTTGGGGGGGAAACCCGCTT‑ACAGGG‑AAATGTGTTACTTTGGGGGGGGGGGTTTACCCGAGAGGAGGCCGGCCCAGGTTGAACACCGCTcc                                                                                      <  2:14456167/134‑1 (MQ=255)

                                                  ctTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCACCC‑ACCAAG‑CAACACGTTACCTCATCAGAGCGGGTTTACCCGCAAAGAGGACAGCCCAGGTTTAACACCACTCCCa                                                                                    >  2:13671527/1‑137 (MQ=255)

                                                     cctcgtccGTCACCGCGGTTGATCCGAACTTGTTCTGTCCTTTCCGCGGTGAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCt                                                                                 <  2:16808378/131‑1 (MQ=37)

                                                     ccGCGGCCGTCACCGCGTTGGATCCGAGCTTGTGCTGTCCTTTTTGCGGGGAAACCCGCCT‑ACCAGG‑CAACGCGTTCCCTTGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCt                                                                                 <  2:15286669/137‑1 (MQ=37)

                                                     ccGCGGCCGCCACCGTGTTTGATCCGAGCTTGGGCCGTCCTCTTCGGGGGCAAACCCGCCG‑ACCAGG‑CAACGCGTTCCCTTGGAGGAGCGGGTTCACCAGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCt                                                                                 <  2:5419191/137‑1 (MQ=37)

                                                           ccGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCACCGGTCAACCCCCCC‑CCCAAG‑CCACCCCTTCCCTCACCAAAACCAGTTTCCCCCCAAAAAAGCCGGCCCCAGTTTAACAACACTCCCAGATccacct                                                                           >  2:17783494/1‑135 (MQ=255)

                                                             gTCACCGCGTTTGATCCAAGCTTTTTCCGGCCTCTTCTCGGTGAAACCCGCCT‑ACCAGA‑CAACCCGCTACCTCGTAGGATCGGGGTTACCCACGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTc                                                                         <  2:17296114/137‑1 (MQ=255)

                                                               cACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCACCC‑CCCCGG‑CAACGCGTTGCCTCGTGGGAGCGGGTTTCCCCGCGAAGAGGCCGGACCCGGTTGAACACCACTCCCAACTGAACTGTCtt                                                                       >  2:18442256/1‑137 (MQ=25)

                                                                           tCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGA‑CAACGCGTGACCCCGTAGGGGCGGGTTAACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCg                                                           <  2:6247662/137‑1 (MQ=38)

                                                                           tCCGAGCGTGTGCCGTCCTTTTTGCGGGCAAACCCCCCT‑ACCAGA‑CAACGCTTTCTCTCGGAGAAGCGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCg                                                           <  1:10964440/137‑1 (MQ=37)

                                                                             tgggCTTGTTCCGGCCTTTTTTTGGGTAAACCCGCTT‑CCCAGG‑CAAGCAGTCCCCTCTTAGGAGCGGATTTACCCGGGAGGAGGCCGGCCCAGGTTGAACACCGCTCCTAGCTATACTGTCTGATCGAGTGGCCGtt                                                         <  2:17679359/134‑1 (MQ=255)

                                                                             cGAACTTGTACCGGCCTCTTCGCGGGGAAACCCGCTG‑ACCAGG‑AAAAGCGTTACCTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGtt                                                         <  2:2127063/137‑1 (MQ=37)

                                                                             aaaGCTTGTGCCGGCCTTTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGGAGGAGTGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGtt                                                         <  2:801367/135‑1 (MQ=255)

                                                                                   tgtgCCGTCCTCTTCGCGGGGAAACCCGCCG‑ACCGGG‑CAACGCGTTACTTTTTAGGAGCGGTTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTc                                                   <  2:16713575/137‑1 (MQ=38)

                                                                                     tgctgtccTTTTCGTGGTTAACCCCGCCT‑ACAAGG‑CAACTGTTTACCTCTGAGGGGTGGTGTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                 <  2:4714070/131‑1 (MQ=255)

                                                                                     tgctgtccTTTTCGTGGGGAACCCCGCCG‑ACCGGA‑AAACGTTTTCCCTTGGGGGGGTGGGTTTACCCGCGAAGGGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                 <  1:2924567/131‑1 (MQ=255)

                                                                                     tgctgtccTTTTCGGGGGTAAACCCTCCC‑CCAAGG‑AAACGCGTCACCTTGGGGGGGAGGTTTTCCCAGCAAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                 <  1:8206239/131‑1 (MQ=255)

                                                                                     tgCTGGCCTTTTCGCGGGGAAACCCGCCT‑ACCAGG‑CAATGTGTGACCTCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                 <  2:13111588/137‑1 (MQ=38)

                                                                                     tgCCGTCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCa                                                 <  2:16242215/137‑1 (MQ=255)

                                                                                      tctggCTTCTGCGTGGGGAAACCCGCCT‑ACAAGG‑CAACGCGTTACCTCGGGGGGGTGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCAt                                                <  2:16604404/134‑1 (MQ=255)

                                                                                             ttttCGTGGGGAAACCCTCCT‑ACCAGA‑CAACGTGTTCCTTTGGGGGAGTGGTTTTACCCGGGAGGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  1:7057426/135‑1 (MQ=255)

                                                                                             ttttCGGGGGGAACCCCGCCT‑ACAAGA‑CAACGCGTTCCCTCGGAGGAGGGGTTTAACCCGAGAAGGGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  1:12793323/135‑1 (MQ=255)

                                                                                             ttttCGCGGGGAAACCCGCCT‑AACAGG‑AAACGCGTTACCTCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  2:14602741/135‑1 (MQ=39)

                                                                                             tctTTGTGGGGAACCCCGCCC‑ACAAGA‑CAACGCGTTCCCTTGGGGGAGTGGGTTCACCCGAGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  2:14526142/137‑1 (MQ=255)

                                                                                             tctTTGGGGGGAACCCCGCCT‑ACCAGG‑AAACTCTGTACCTTGTGGGGTTGGGTTTACCTTCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  1:7464432/137‑1 (MQ=255)

                                                                                             tctTTGGGGGGAAACCCGCCG‑ACAAGA‑AAACGCTTTACCTCGTGGGAGCGGGTTCACCAGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  1:3734015/137‑1 (MQ=37)

                                                                                             tctTCGGGGGTAAACCCCCCT‑ACAAGG‑CAACGCGTTACCTCGGAGGAGCGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  1:3109664/137‑1 (MQ=39)

                                                                                             tctTCGGGGGG‑AACCCCTCT‑CCCAGGACAACCTGTTACCTTGGGGGGGTGGTTTTCCCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  1:6666027/137‑1 (MQ=255)

                                                                                             tctTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  2:3637928/137‑1 (MQ=255)

                                                                                             tctTCGCGGGTAAACCCGCCT‑ACCAGG‑AAACGCGTTACCTCGTAGGAGCGGTTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  2:17446344/137‑1 (MQ=255)

                                                                                             tctTAGGGGGGAAACCCGCCT‑ACAGGA‑CAACTCGTTACCTTGGAGGAGTGGGTTTACCCGAGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCt                                         <  1:3829037/137‑1 (MQ=255)

                                                                                              ctTCACGGGTAAACCCGCCT‑ACCGGG‑AAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCtt                                        <  2:7765205/137‑1 (MQ=255)

                                                                                                  gcgGGTAAACCCCCCC‑ACCAGG‑CAATGCGTCACCTTGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACaa                                    <  1:3319524/137‑1 (MQ=38)

                                                                                                  gcgGGGAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCTGAGGAGCGGGTTTACCCGAGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACaa                                    <  2:3088737/137‑1 (MQ=255)

                                                                                                     gggaaaCCCGCCG‑ACCAGG‑AAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAagag                                 <  2:17115205/134‑1 (MQ=255)

                                                                                                      ggaaccccGCCT‑ACCAGA‑CAACGCTTTCCCTCGGGGGAGTGGGTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGc                                <  1:10119163/132‑1 (MQ=255)

                                                                                                           cccGCCC‑ACCGGG‑AAACGTGTTACCTTGGGGGAGTGGTTTCACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGac                           <  2:5727922/137‑1 (MQ=255)

                                                                                                              gCCT‑ACCAGG‑CAACGCGTTACCTCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGaccac                        <  2:12348523/137‑1 (MQ=255)

                                                                                                                    ccAGG‑CAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGcc                   >  2:485702/1‑137 (MQ=255)

                                                                                                                    ccAGG‑CAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGcc                   >  1:16769393/1‑137 (MQ=255)

                                                                                                                    ccAGG‑CAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGcc                   >  1:562709/1‑137 (MQ=255)

                                                                                                                    ccAGG‑CAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGcc                   >  1:9674221/1‑137 (MQ=255)

                                                                                                                    ccAGG‑CAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGcc                   >  1:10711190/1‑137 (MQ=255)

                                                                                                                           aatgtgTTACCTTGGGGGAGTGGGTTTACCCGAGAAGGGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc             <  2:8112121/132‑1 (MQ=255)

                                                                                                                           aaCGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGaa                                                                                               >  1:10256571/1‑55 (MQ=255)

                                                                                                                           aaCGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGaa                                                                                               >  1:803816/1‑55 (MQ=255)

                                                                                                                           aaCGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGaa                                                                                               <  2:10256571/55‑1 (MQ=255)

                                                                                                                           aaCGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc             >  1:12497850/1‑137 (MQ=255)

                                                                                                                             cgcgTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCACGTTCGa           >  2:5184464/1‑137 (MQ=255)

                                                                                                                              gcgTTACCTCGGAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGat          <  2:16433399/137‑1 (MQ=255)

                                                                                                                               cgTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGata         >  2:4453706/1‑137 (MQ=255)

                                                                                                                                tttACCTCTTGGGAGCGGGTTTACCCGTGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat        <  2:9499208/136‑1 (MQ=255)

                                                                                                                                gTTACTTTGGAGGAGCGGGTTTACCCGCGAGGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat        <  2:18022370/137‑1 (MQ=255)

                                                                                                                                gTTACCTCGTAGGAGTGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat        <  1:13747893/137‑1 (MQ=255)

                                                                                                                                gTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat        >  1:8877439/1‑137 (MQ=255)

                                                                                                                                gTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat        >  2:1558988/1‑137 (MQ=255)

                                                                                                                                gTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat        >  1:4682944/1‑137 (MQ=255)

                                                                                                                                    ccTCGGAGGAGCGGTTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGc    <  2:18690577/137‑1 (MQ=255)

                                                                                                                                    ccTCGGAGGAGCGGGTTTACCCGCGAGGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGc    <  1:3534926/137‑1 (MQ=255)

                                                                                                                                     ttCGTAGGAGTGGTTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa   <  2:11955626/136‑1 (MQ=255)

                                                                                                                                     cTCGTAGGAGCGGGTTTACCCTCGAAGAGGCCGGCCCAGGTTGAACACCGCTTCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCa   <  2:10805106/137‑1 (MQ=255)

                                                                                                                                      tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGTTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa  >  2:16103584/1‑137 (MQ=255)

                                                                                                                                      tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTNTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa  >  1:17481900/1‑137 (MQ=255)

                                                                                                                                      tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa  >  1:13225916/1‑137 (MQ=255)

                                                                                                                                      tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa  >  2:907387/1‑137 (MQ=255)

                                                                                                                                      tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa  >  2:12516945/1‑137 (MQ=255)

                                                                                                                                      tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaa  >  2:4145966/1‑137 (MQ=255)

AGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCT‑ACCAGG‑CAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAA  >  AE015451_phaM1/5344351‑5344619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`