Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 144,577 C→T intergenic (+556/‑190) PP_0136 CDS (conserved exported protein of unknown function) → / → gltP CDS (glutamate_aspartate‑proton DAACS transporter) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1144,5770CT100.0% 3272.3 / NA 888intergenic (+556/‑190)PP_0136 CDS (conserved exported protein of unknown function)/gltP CDS (glutamate_aspartate‑proton DAACS transporter)–/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (401/487);  total (401/487)

Only 100 of 888 total aligned reads displayed.

GTCCCGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGCATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGCCCATG  >  AE015451_phaM1/144444‑144709
                                                                                                                                     |                                                                                                                                    
gTCCTGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTAtt                                                                                                                                   >  2:18946311/1‑137 (MQ=255)
gTCCCGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTAtt                                                                                                                                   >  1:18605610/1‑137 (MQ=255)
gTCCCGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTAtt                                                                                                                                   >  1:6117406/1‑137 (MQ=255)
gTCCCGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTAtt                                                                                                                                   >  1:11888760/1‑137 (MQ=255)
gTCCCGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTAtt                                                                                                                                   >  2:18046539/1‑137 (MQ=255)
   ccGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGc                                                                                                                                <  1:4284029/137‑1 (MQ=255)
     gCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCtt                                                                                                                              >  1:18875995/1‑137 (MQ=255)
      ccAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCttt                                                                                                                             >  2:9757175/1‑137 (MQ=255)
      ccAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCttt                                                                                                                             >  2:9203401/1‑137 (MQ=255)
       cAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTc                                                                                                                            <  1:14161556/137‑1 (MQ=255)
       cAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTc                                                                                                                            >  1:9020501/1‑137 (MQ=255)
       cAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGAATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTc                                                                                                                            >  2:17348267/1‑137 (MQ=255)
        aaaTCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCg                                                                                                                           >  1:833708/1‑137 (MQ=255)
         aaTCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGc                                                                                                                          >  2:9032537/1‑137 (MQ=255)
         aaTCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGc                                                                                                                          >  1:3731156/1‑137 (MQ=255)
         aaTCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGAACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGc                                                                                                                          >  2:18662099/1‑137 (MQ=255)
           tCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAGACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGccc                                                                                                                        <  2:12858744/137‑1 (MQ=255)
            cTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGcccc                                                                                                                       <  2:17652283/137‑1 (MQ=255)
             tGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCt                                                                                                                      <  1:9238056/137‑1 (MQ=255)
                   aaTATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGt                                                                                                                >  2:8568738/1‑137 (MQ=255)
                                       ttCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGTTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCtttt                                                                                            <  2:13675081/137‑1 (MQ=255)
                                       ttCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCtttt                                                                                            <  2:15752849/137‑1 (MQ=255)
                                         cGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCATTTTTc                                                                                          <  2:2307565/137‑1 (MQ=255)
                                          tggATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATGGTTGCCGCCTTTTTCg                                                                                         <  1:10243800/136‑1 (MQ=255)
                                          gggATTCCGAATGTTATAAATCAAGTACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCg                                                                                         <  2:15447921/137‑1 (MQ=255)
                                          gggATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCg                                                                                         <  1:13012777/137‑1 (MQ=255)
                                          gggATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCg                                                                                         <  2:4486301/137‑1 (MQ=255)
                                          gggATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCg                                                                                         <  1:5813408/137‑1 (MQ=255)
                                          gggATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCg                                                                                         <  2:16520662/137‑1 (MQ=255)
                                          gggATTCCGAATGGTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCg                                                                                         <  2:14191813/137‑1 (MQ=255)
                                              ttCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTgg                                                                                     <  2:2109109/137‑1 (MQ=255)
                                               tCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGc                                                                                    >  1:6553113/1‑137 (MQ=255)
                                               tCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGc                                                                                    >  1:7750777/1‑137 (MQ=255)
                                                ccGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGcc                                                                                   <  1:12688334/137‑1 (MQ=255)
                                                  gAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCag                                                                                 <  1:8074258/137‑1 (MQ=255)
                                                    aTGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCagag                                                                               >  2:13523712/1‑137 (MQ=255)
                                                           aaaTCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTg                                                                        >  1:3362021/1‑137 (MQ=255)
                                                           aaaTCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTg                                                                        <  2:16751741/137‑1 (MQ=255)
                                                           aaaTCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTg                                                                        <  1:16883127/137‑1 (MQ=255)
                                                           aaaTCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTg                                                                        >  2:12495184/1‑137 (MQ=255)
                                                             aTCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGtc                                                                      <  2:6383324/137‑1 (MQ=255)
                                                                aaGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGtcgct                                                                   <  2:10228899/137‑1 (MQ=255)
                                                                    aCTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGcc                                                               >  2:14786801/1‑137 (MQ=255)
                                                                    aCTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGcc                                                               >  2:14532200/1‑137 (MQ=255)
                                                                    aCTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGcc                                                               >  2:18317505/1‑137 (MQ=255)
                                                                    aCTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGcc                                                               >  2:7370231/1‑137 (MQ=255)
                                                                    aCTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGcc                                                               >  2:7649709/1‑137 (MQ=255)
                                                                    aCTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTAAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGcc                                                               >  2:11004968/1‑137 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  1:9460604/137‑1 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  1:10780408/137‑1 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  1:14541253/137‑1 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  1:5324394/137‑1 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  1:11425020/137‑1 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  2:3081564/137‑1 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  2:4995272/137‑1 (MQ=255)
                                                                     cTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCt                                                              <  2:5373769/137‑1 (MQ=255)
                                                                      ttGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTg                                                             <  2:16265457/137‑1 (MQ=255)
                                                                      ttGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTg                                                             <  1:4622672/137‑1 (MQ=255)
                                                                           aaGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGgtg                                                        >  2:15072832/1‑137 (MQ=255)
                                                                             gCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTc                                                      >  1:18351530/1‑137 (MQ=255)
                                                                              cTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGNCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTcc                                                     <  1:16339994/137‑1 (MQ=255)
                                                                                   ggCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACa                                                >  1:12488413/1‑137 (MQ=255)
                                                                                     cTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGNTCGCCTGCGGTGTCCTTACATg                                              >  2:8800861/1‑137 (MQ=255)
                                                                                          tCGAAAACAGTATGCATAACCGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTc                                         <  1:7428921/137‑1 (MQ=255)
                                                                                          tCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTc                                         <  1:7133484/137‑1 (MQ=255)
                                                                                          tCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTc                                         <  1:3011004/137‑1 (MQ=255)
                                                                                              aaaCAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGGGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCt                                     <  1:13349664/137‑1 (MQ=255)
                                                                                               aaCAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTg                                    <  1:1775197/137‑1 (MQ=255)
                                                                                                 cAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGAc                                  <  1:9822446/137‑1 (MQ=255)
                                                                                                 cAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGAc                                  <  2:8164192/137‑1 (MQ=255)
                                                                                                       gCATAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGc                            >  1:1710688/1‑137 (MQ=255)
                                                                                                         aTAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCt                          <  2:6157838/137‑1 (MQ=255)
                                                                                                          tAAACGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCtt                         <  1:2177072/137‑1 (MQ=255)
                                                                                                            aaCGGCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTa                       >  2:11401053/1‑137 (MQ=255)
                                                                                                               ggCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTg                    <  1:6310720/137‑1 (MQ=255)
                                                                                                               ggCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTg                    <  1:8797937/137‑1 (MQ=255)
                                                                                                               ggCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTg                    >  2:3650488/1‑137 (MQ=255)
                                                                                                                gCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGc                   <  1:10002141/137‑1 (MQ=255)
                                                                                                                gCATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGc                   <  2:2909912/137‑1 (MQ=255)
                                                                                                                 cATGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGcc                  >  1:15506565/1‑137 (MQ=255)
                                                                                                                  aTGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGccc                 >  2:4706092/1‑137 (MQ=255)
                                                                                                                   tGGGCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCg                >  2:4055882/1‑137 (MQ=255)
                                                                                                                     ggCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTg              <  2:11810169/137‑1 (MQ=255)
                                                                                                                     ggCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTg              >  1:17931929/1‑137 (MQ=255)
                                                                                                                     ggCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTg              <  2:677690/137‑1 (MQ=255)
                                                                                                                     ggCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTg              >  1:13725103/1‑137 (MQ=255)
                                                                                                                     ggCTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCATGACGGAAGCCTTTATTGCCCGTg              >  1:1219358/1‑137 (MQ=255)
                                                                                                                       cTATGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGAc            >  1:11061744/1‑137 (MQ=255)
                                                                                                                          tGCGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCtt         <  1:15112642/137‑1 (MQ=255)
                                                                                                                            cGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGc       <  2:5555982/137‑1 (MQ=255)
                                                                                                                            cGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGc       <  2:13202846/137‑1 (MQ=255)
                                                                                                                            cGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGc       <  1:15945552/137‑1 (MQ=255)
                                                                                                                            cGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGc       <  2:2655414/137‑1 (MQ=255)
                                                                                                                            cGTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGc       >  2:15900357/1‑137 (MQ=255)
                                                                                                                             gTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGc                   >  2:3428993/1‑124 (MQ=255)
                                                                                                                             gTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGcc      >  2:12254309/1‑137 (MQ=255)
                                                                                                                             gTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGcc      >  2:7178609/1‑137 (MQ=255)
                                                                                                                             gTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGcc      >  1:2600661/1‑137 (MQ=255)
                                                                                                                             gTTTCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTATCGTCCGCCTACGGTGTCCTTACATGACGTCGCCTGACGGAAGCATTTATTGCCCGTGACCTTGca      >  2:10083094/1‑136 (MQ=255)
                                                                                                                              tttCAGGTATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGccc     <  2:10560466/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GTCCCGCCAAATCTGCCGAAATATGGCGGTTTGGTCTTGTTCGGGATTCCGAATGTTATAAATCAAGCACTTGCGAAGCTGCCGGCTAGGTCGAAAACAGTATGCATAAACGGCATGGGCTATGCGTTTCAGGCATTAGCTTTCGCCCCTTGCCGTCGCAATAGTTGCCGCCTTTTTCGCTGGCCAGAGCGCCCTGTCGCTCGCCTGCGGTGTCCTTACATGACGTCGCCTGACGGAAGCCTTTATTGCCCGTGACCTTGCCCATG  >  AE015451_phaM1/144444‑144709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑