BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	1,713,060	A→G	T2A (ACC→GCC)	tsaB CDS (endopeptidase) →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,713,060	0	A	G	99.6%	3293.1 / ‑5.3	812	T2A (ACC→GCC)	tsaB CDS (endopeptidase)
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (457/351); total (459/353)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 816 total aligned reads displayed.

ACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712929‑1713192

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTCTGCCGCACAGCGCCCCCTCATGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  2:7251814/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:366632/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:10548039/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  2:7573261/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:12558756/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  2:15084208/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACATGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:1609958/137‑1 (MQ=255)

   cACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctg                                                                                                                              <  1:1077538/137‑1 (MQ=255)

   cACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctg                                                                                                                              <  2:8268330/137‑1 (MQ=255)

      cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctg                                                                                                                           <  2:6116290/137‑1 (MQ=255)

      cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctg                                                                                                                           <  1:13697082/137‑1 (MQ=255)

         gACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGcc                                                                                                                        >  2:14573992/1‑137 (MQ=255)

           cAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCt                                                                                                                      <  1:100401/137‑1 (MQ=255)

                gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                 <  2:10037286/137‑1 (MQ=255)

                gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                 <  1:11592663/137‑1 (MQ=255)

                gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                 <  1:16894373/137‑1 (MQ=255)

                 cccGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATAc                                                                                                                >  2:2942312/1‑137 (MQ=255)

                 cccGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATAc                                                                                                                >  2:438094/1‑137 (MQ=255)

                   cGCTTGCGGGCCATTGTCGTTTGTAGGCCGCACAGCGCCCCCCCAGGACACAACACCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACACGTTCGATGGCCACCCTGCTGGCACTGGATACCg                                                                                                              >  1:16521057/1‑137 (MQ=255)

                     cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  2:5243940/137‑1 (MQ=255)

                     cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  1:7785545/137‑1 (MQ=255)

                     cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  2:9486717/137‑1 (MQ=255)

                     cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  1:17060901/137‑1 (MQ=255)

                        gCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAcc                                                                                                         >  2:3520955/1‑137 (MQ=255)

                                     gTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCg                                                                                            <  1:395337/137‑1 (MQ=255)

                                      tttGTATGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGt                                                                                           <  2:16938680/137‑1 (MQ=255)

                                       ttGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGACACCGAAGCCTGTTCCGTc                                                                                          <  1:15119610/137‑1 (MQ=255)

                                         gTAGGCCGCACAGCGTCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTcgc                                                                                        >  2:5054838/1‑137 (MQ=255)

                                          tAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTcgcg                                                                                       <  1:12901079/137‑1 (MQ=255)

                                           aGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCgcgc                                                                                      <  2:13851772/137‑1 (MQ=255)

                                           aGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCgcgc                                                                                      >  1:14181825/1‑137 (MQ=255)

                                               cGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCTAAGCCTGTTCCGTCGCgctgct                                                                                  <  2:18869060/137‑1 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  1:512138/1‑137 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  1:14805413/1‑137 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:8097197/1‑137 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  1:15880505/1‑137 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  1:81410/1‑137 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:1108131/1‑137 (MQ=255)

                                                      cgcCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGAc                                                                           <  2:676023/137‑1 (MQ=255)

                                                         ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  2:17974566/1‑137 (MQ=255)

                                                         ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  1:7094553/1‑137 (MQ=255)

                                                         ccccTCAGGACACAACACCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  2:3948653/1‑137 (MQ=255)

                                                         ccccTCAGGACACAACACCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  2:8782060/1‑137 (MQ=255)

                                                             tCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAgg                                                                    <  2:899611/137‑1 (MQ=255)

                                                              cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  1:12094461/137‑1 (MQ=255)

                                                              cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  2:12802546/137‑1 (MQ=255)

                                                               aGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTg                                                                  <  1:4577709/137‑1 (MQ=255)

                                                                 gACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAc                                                                <  1:4934094/137‑1 (MQ=255)

                                                                       aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  1:1787316/137‑1 (MQ=255)

                                                                       aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  1:11212157/137‑1 (MQ=255)

                                                                         tccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        <  1:9501953/136‑1 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  1:15353721/1‑137 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  1:13381098/1‑137 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  1:892299/1‑137 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  1:6624918/1‑137 (MQ=255)

                                                                          ccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTa                                                       >  2:15652975/1‑137 (MQ=255)

                                                                              tgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAg                                                   >  2:14146753/1‑137 (MQ=255)

                                                                                      tataCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATccc                                           <  2:6885145/137‑1 (MQ=255)

                                                                                          cTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCgcgc                                       <  1:697707/137‑1 (MQ=255)

                                                                                           tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgca                                      <  1:13163995/137‑1 (MQ=255)

                                                                                           tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgca                                      >  2:15269748/1‑137 (MQ=255)

                                                                                           tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgca                                      >  1:4368143/1‑137 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  2:640325/137‑1 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  2:18214036/137‑1 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  1:8466386/137‑1 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  2:7794905/137‑1 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCANCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:17507948/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAATCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:17347359/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:981458/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:3429900/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:5496082/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:9337036/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:5796267/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:3654104/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:14536254/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:2531715/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:13135910/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:12342844/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAACAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:11569307/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGCTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAACAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:1046333/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:16793412/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:18550618/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:4672436/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:2266418/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:12031001/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAACAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:16204017/1‑137 (MQ=255)

                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGGTCCCGCGCATGCATGCCCa                            >  1:10323768/1‑137 (MQ=255)

                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:5571676/1‑137 (MQ=255)

                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  1:2660838/1‑137 (MQ=255)

                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  1:18185854/137‑1 (MQ=255)

                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  1:7373710/137‑1 (MQ=255)

                                                                                                              cACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAgctgct                   >  1:6099389/1‑137 (MQ=255)

                                                                                                                ccTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCtgctgc                 >  2:3255060/1‑137 (MQ=255)

                                                                                                                ccTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCtgctgc                 <  2:13706525/137‑1 (MQ=255)

                                                                                                                ccTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCtgctgc                 >  2:6650681/1‑137 (MQ=255)

                                                                                                                    gATACCCGTTCGAGGGCCACTCTGCTGACCTTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGATGTGATCCCGCGCATGCATGCCCAGAAGCTACTGCCgat             <  2:17578577/137‑1 (MQ=255)

                                                                                                                         ccGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCa        <  1:2088756/137‑1 (MQ=255)

                                                                                                                           gTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAg      >  2:10662987/1‑137 (MQ=255)

                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  2:8500241/1‑137 (MQ=255)

                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  1:9140306/1‑137 (MQ=255)

ACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712929‑1713192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`