BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	4,741,294	C→G	intergenic (+531/+222)	gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,741,294	0	C	G	100.0%	402.8 / NA	101	intergenic (+531/+222)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (56/45); total (56/45)

Only 100 of 101 total aligned reads displayed.

GAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACC‑TCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGC  >  AE015451_phaM1/4741187‑4741398

gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                              >  2:9168592/1‑137 (MQ=255)

gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                              <  1:1495419/137‑1 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  2:14358006/1‑137 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  2:7960132/1‑137 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  2:10478291/1‑137 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  2:10478250/1‑137 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  1:18602202/1‑137 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  1:13363157/1‑137 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  2:6605224/1‑137 (MQ=255)

 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                             >  1:5805458/1‑137 (MQ=255)

  aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                            <  1:16988682/137‑1 (MQ=255)

  aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                            <  1:18942677/137‑1 (MQ=255)

  aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                            <  1:7137337/137‑1 (MQ=255)

  aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                            <  2:5517157/137‑1 (MQ=255)

  aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTCTGCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                            <  1:3306998/137‑1 (MQ=255)

  aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACATGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                            <  2:12568127/137‑1 (MQ=255)

   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                           <  2:18878761/137‑1 (MQ=255)

   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                           <  1:17722279/137‑1 (MQ=255)

   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                           <  1:1145707/137‑1 (MQ=255)

   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGTACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                           <  1:18961090/137‑1 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  2:5195707/1‑137 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  1:6338841/1‑137 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  2:5870818/1‑137 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  2:6745464/1‑137 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  1:15859094/1‑137 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  2:14428294/1‑137 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  2:10675537/1‑137 (MQ=255)

      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                                        >  2:5870778/1‑137 (MQ=255)

       gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                                       >  1:10263982/1‑137 (MQ=255)

         atatttCCAGCTCTGGTCGCCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGATACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  2:10356196/132‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTTGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:2315996/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:8414392/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:7987193/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:9131656/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:7987160/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:10741043/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  2:14052537/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:17438991/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:17438992/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:18526243/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:18526287/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  2:2642543/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  2:4306392/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  2:4978183/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:7294476/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:7913346/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:7993254/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:13191722/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:2225172/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:14678053/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:3497055/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:13970889/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:4658548/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:17452356/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:13271468/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:6580696/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:17050563/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:7340470/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:7528140/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:1196630/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:17341335/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:4187319/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  1:1865541/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CCGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCAAGCGGCGCCTTGATCACGGCCACACcgc                                                                     >  1:7528179/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGACTTGC‑ATTCGTGAATCCATGC‑ACATGCCCCGGACACGTCCTCCTACAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     <  2:7730138/137‑1 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  2:17438954/1‑137 (MQ=255)

         ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                     >  1:9511285/1‑137 (MQ=255)

          taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                                    <  2:1354999/137‑1 (MQ=255)

          taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                                    >  2:3172020/1‑137 (MQ=255)

            ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                                                  >  2:4827788/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCTTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  2:4377187/137‑1 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                >  1:18666551/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  1:17715009/137‑1 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  2:6321729/137‑1 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                >  1:13177999/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                >  1:11269786/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                >  1:7646517/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                >  1:7646467/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                >  1:5516723/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  2:1091589/137‑1 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  2:1266666/137‑1 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  1:9266605/137‑1 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  1:14713601/137‑1 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                >  2:1551267/1‑137 (MQ=255)

              tCCAGCTCTGGTCACCTTCGTACTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGGCCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                                <  2:15013714/137‑1 (MQ=255)

               ccAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                               <  1:200713/137‑1 (MQ=255)

                cAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                              <  1:7931931/137‑1 (MQ=255)

                cAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                              <  1:13872157/137‑1 (MQ=255)

                cAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                              <  1:2078294/137‑1 (MQ=255)

                cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGTGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                              >  2:8016632/1‑137 (MQ=255)

                cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                              <  2:4693737/137‑1 (MQ=255)

                cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                              <  1:8363108/137‑1 (MQ=255)

                cAGCTCTGGTCACCTTCGTGCTTGAACAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                              <  2:10015644/137‑1 (MQ=255)

                   ctctGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                           >  1:4833220/1‑137 (MQ=255)

                   ctctGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTAACACCGCGATAGGCCtt                                                           >  2:18892969/1‑137 (MQ=255)

                   ctctGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TNGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                           >  1:18302307/1‑137 (MQ=255)

                     ctGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                         >  1:12702846/1‑137 (MQ=255)

                     ctGGTCACCTTCGTGCTTGAGCAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGTGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                         >  2:16923628/1‑137 (MQ=255)

                     ctGGTCACCTTCGTGCTTGAG‑CAGC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                         >  1:15946509/1‑137 (MQ=255)

                                                                                 caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCAAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTAACTGAGGGACACCTTGCTCCCGTACTCGTCCTTAGc  >  1:14151836/2‑137 (MQ=255)

GAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACC‑TCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGC  >  AE015451_phaM1/4741187‑4741398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`