Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,660,005 A→C H350P (CAC→CCC)  metK CDS (methionine adenosyltransferase) →

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,660,0050AC100.0% 2828.9 / NA 699H350P (CAC→CCC) metK CDS (methionine adenosyltransferase)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (263/436);  total (263/436)

Only 100 of 701 total aligned reads displayed.

CGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTG  >  AE015451_phaM1/5659871‑5660136
                                                                                                                                      |                                                                                                                                   
 gTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCcccc                                                                                                                                  >  1:12221324/1‑137 (MQ=255)
   ggCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGa                                                                                                                                <  1:16992907/137‑1 (MQ=255)
       cAGCCGACCTCCATCTCGATCACCACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTa                                                                                                                            >  1:2543005/1‑137 (MQ=255)
       cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCTATCACCACCATGCTCGACCTGCTGCCCCCGATGTa                                                                                                                            >  1:342234/1‑137 (MQ=255)
       cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATg                                                                                                                              >  2:7384051/1‑135 (MQ=255)
       cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTa                                                                                                                            >  2:12103792/1‑137 (MQ=255)
       cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTa                                                                                                                            >  1:17262794/1‑137 (MQ=255)
         gCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                          <  2:1706487/137‑1 (MQ=255)
          ccGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCTGACGACAAGATCATTCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCa                                                                                                                         <  2:12853717/137‑1 (MQ=255)
          ccGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCa                                                                                                                         <  1:16545768/137‑1 (MQ=255)
          ccGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCa                                                                                                                         <  2:17937294/137‑1 (MQ=255)
            gACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAgg                                                                                                                       >  1:12686942/1‑137 (MQ=255)
               cTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGaaa                                                                                                                    <  1:5102282/137‑1 (MQ=255)
               cTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGaaa                                                                                                                    <  2:11200333/137‑1 (MQ=255)
                  cATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCg                                                                                                                 >  1:5515269/1‑137 (MQ=255)
                     ctcGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTg                                                                                                              >  2:17285581/1‑137 (MQ=255)
                       cGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGcc                                                                                                            >  2:1347464/1‑137 (MQ=255)
                            aaCACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACgg                                                                                                       >  2:326602/1‑137 (MQ=255)
                            aaCACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACgg                                                                                                       <  1:5295751/137‑1 (MQ=255)
                            aaCACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACgg                                                                                                       >  1:6145658/1‑137 (MQ=255)
                            aaCACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACgg                                                                                                       <  2:14350644/137‑1 (MQ=255)
                            aaCACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACgg                                                                                                       >  2:10844715/1‑137 (MQ=255)
                             acacCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGc                                                                                                      <  1:7964697/137‑1 (MQ=255)
                             acacCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGc                                                                                                      <  2:11426685/137‑1 (MQ=255)
                               acCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCa                                                                                                    <  2:7698409/137‑1 (MQ=255)
                                    cGGTACCGGCAAGGTCGCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCg                                                                                               <  2:16749281/137‑1 (MQ=255)
                                       tACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGcc                                                                                            >  1:8703945/1‑137 (MQ=255)
                                       tACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGcc                                                                                            <  1:18203436/137‑1 (MQ=255)
                                          cGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTa                                                                                         <  2:2002845/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  1:6472183/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  1:10115716/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  2:2849507/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  2:15112893/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  2:9150112/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  2:5368635/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  1:15528825/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  1:15687350/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  1:15993217/137‑1 (MQ=255)
                                            gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCGGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  1:14421048/137‑1 (MQ=255)
                                               aGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCg                                                                                    >  1:11578550/1‑137 (MQ=255)
                                               aGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCg                                                                                    >  2:8819498/1‑137 (MQ=255)
                                               aGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCg                                                                                    >  1:9737119/1‑137 (MQ=255)
                                               aGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCg                                                                                    <  2:477661/137‑1 (MQ=255)
                                                    tcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCgcagca                                                                               >  1:11210302/1‑137 (MQ=255)
                                                    tcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCgcagca                                                                               >  1:2363795/1‑137 (MQ=255)
                                                      cgacgaCAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCaga                                                                             <  2:636970/137‑1 (MQ=255)
                                                         cgacAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCagaaga                                                                          >  1:17812954/1‑137 (MQ=255)
                                                             aaGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGt                                                                      <  2:2667489/137‑1 (MQ=255)
                                                               gATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCg                                                                    >  2:18905600/1‑137 (MQ=255)
                                                               gATCATCCAGCTGGCGCGCGAGTGCTTCGACCTGCGTCCATACGCCATCACCACCACGCTCGACCTGCTGCCCCCGACGTATCAGAAAACCGCTGCCTACGGCAACTGCGGCCGTACTCAGCAAAAGAAAACTATCg                                                                    >  2:16482594/1‑137 (MQ=255)
                                                                 tcatcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGc                                                                  <  1:4669001/137‑1 (MQ=255)
                                                                  catcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCTGCCGTACTCCGCAGCAGAAGACTGTCGGcg                                                                 <  2:14809322/137‑1 (MQ=255)
                                                                  catcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcg                                                                 <  1:6866819/137‑1 (MQ=255)
                                                                  catcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcg                                                                 <  2:7851943/137‑1 (MQ=255)
                                                                   atcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcga                                                                <  2:5889876/137‑1 (MQ=255)
                                                                    tcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcgac                                                               <  1:2022342/137‑1 (MQ=255)
                                                                     ccAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcgacg                                                              <  1:1094574/137‑1 (MQ=255)
                                                                           ggTGCGCGAGTGTTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                        <  2:10097020/137‑1 (MQ=255)
                                                                           ggTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                        <  2:10527892/137‑1 (MQ=255)
                                                                           ggTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                        <  1:13872189/137‑1 (MQ=255)
                                                                           ggTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                        <  1:18546813/137‑1 (MQ=255)
                                                                           ggTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                        <  1:2153658/137‑1 (MQ=255)
                                                                            gTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacctt                                                       <  1:9172211/137‑1 (MQ=255)
                                                                             tGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttc                                                      >  2:18468457/1‑137 (MQ=255)
                                                                               cgcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcac                                                    <  1:10688372/137‑1 (MQ=255)
                                                                               cgcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcac                                                    <  2:4665403/137‑1 (MQ=255)
                                                                               cgcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcac                                                    <  1:27642/137‑1 (MQ=255)
                                                                                gcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcacc                                                   >  1:3025012/1‑137 (MQ=255)
                                                                                gcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcacc                                                   >  2:2316907/1‑137 (MQ=255)
                                                                                 cgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcacca                                                  <  1:8324187/137‑1 (MQ=255)
                                                                                    gTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGTCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcaccacct                                               >  2:15371027/1‑137 (MQ=255)
                                                                                      gCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcaccaccttc                                             >  1:13176688/1‑137 (MQ=255)
                                                                                      gCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcaccaccttc                                             <  2:11864210/137‑1 (MQ=255)
                                                                                         tCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACaccttcaccaccttcacc                                          >  2:13775900/1‑137 (MQ=255)
                                                                                          cGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCt                                         >  2:9504323/1‑137 (MQ=255)
                                                                                          cGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCt                                         >  2:6727511/1‑137 (MQ=255)
                                                                                                    ccGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGNCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCac                               <  1:17526695/137‑1 (MQ=255)
                                                                                                    ccGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGTAGAAGACTGTCGGCGACGACACCTTCACCACCTTCCCCTGGGAGCGCac                               >  1:11373149/1‑137 (MQ=255)
                                                                                                    ccGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCac                               >  1:14693177/1‑137 (MQ=255)
                                                                                                    ccGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCac                               >  1:14941416/1‑137 (MQ=255)
                                                                                                    ccGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCac                               >  2:17793880/1‑137 (MQ=255)
                                                                                                     cGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                              <  2:13338171/137‑1 (MQ=255)
                                                                                                         cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCaccgacc                          <  1:13721759/137‑1 (MQ=255)
                                                                                                         cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCaccgacc                          <  1:5772326/137‑1 (MQ=255)
                                                                                                         cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCaccgacc                          <  1:7113656/137‑1 (MQ=255)
                                                                                                           ccATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                        <  1:4309257/137‑1 (MQ=255)
                                                                                                           ccATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                        <  1:16756603/137‑1 (MQ=255)
                                                                                                                 ccaccaTGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAg                  >  1:7387023/1‑137 (MQ=255)
                                                                                                                 ccaccaTGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAg                  >  1:7687762/1‑137 (MQ=255)
                                                                                                                   accaTGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                       <  1:18806363/66‑1 (MQ=255)
                                                                                                                       tGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTg            >  1:3748039/1‑137 (MQ=255)
                                                                                                                        gCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTAGGAGCGCACCGACCGCGCCCAGTCGTTGc           >  2:12356098/1‑137 (MQ=255)
                                                                                                                         cTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCg          <  1:15154503/137‑1 (MQ=255)
                                                                                                                         cTCGACCGGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCg          <  2:8370691/137‑1 (MQ=255)
                                                                                                                           cGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                              >  1:13397199/1‑115 (MQ=255)
                                                                                                                            gACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                        >  2:3569634/1‑88 (MQ=255)
                                                                                                                            gACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGa       >  2:4056047/1‑137 (MQ=255)
                                                                                                                               ctgctgCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGc    >  1:11880659/1‑137 (MQ=255)
                                                                                                                               ctgctgCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGc    <  1:9510801/137‑1 (MQ=255)
                                                                                                                               ctgctgCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGc    <  2:15816524/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
CGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTG  >  AE015451_phaM1/5659871‑5660136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑