Predicted mutation
evidence seq id position mutation annotation gene description
RA pS4413_qbsFGH 106 C→T intergenic (–/‑3256) – / → aadA CDS –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*pS4413_qbsFGH1060CT99.9% 38346.4 / NA 10412intergenic (–/‑3256)–/aadA CDS–/–
Reads supporting (aligned to +/- strand):  ref base C (9/4);  new base T (7051/3336);  total (7066/3346)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 10414 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGATAAAGTGCT  >  pS4413_qbsFGH/1‑239
                                                                                                                                  |                                                                                                                                     
gagcctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTAcc                                                                                                                                 >  1:14953028‑M1/26‑137 (MQ=255)
gagcctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTAcc                                                                                                                                 >  1:4155950‑M1/26‑137 (MQ=255)
 agcctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCa                                                                                                                                >  1:2886053‑M1/25‑137 (MQ=255)
   cctttcgttttatttgatgcctTTAATTCATTGTTGACAATTAATCATCGACATAGTATATCGGCATCGTATAATACGACAAGGTGCGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACAagg                                                                                                                              <  2:4222650‑M1/115‑1 (MQ=255)
   cctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCagg                                                                                                                              <  1:18658019‑M1/115‑1 (MQ=255)
    ctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCagga                                                                                                                             <  2:2123513‑M1/116‑1 (MQ=255)
    ctttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCagga                                                                                                                             <  1:6225162‑M1/116‑1 (MQ=255)
      ttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGNCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCaggagg                                                                                                                           >  1:15326560‑M1/20‑137 (MQ=255)
      ttcgttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCaggagg                                                                                                                           >  2:16025754‑M1/20‑137 (MQ=255)
         gttttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGaaa                                                                                                                        <  1:1267530‑M1/121‑1 (MQ=255)
           tttatttgatgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGaaaaa                                                                                                                      <  1:5838326‑M1/123‑1 (MQ=255)
                    tgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCATGAGGAAAAACATATGTGc                                                                                                             >  2:13389671‑M1/6‑137 (MQ=255)
                    tgcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGc                                                                                                             >  2:5604870‑M1/6‑137 (MQ=255)
                    tgcctTTAAGTAGTTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTTGAGCTTGGTACCAGGAGGAAAAACATATGTGc                                                                                                             >  1:12346710‑M1/6‑137 (MQ=255)
                     gcctTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGcc                                                                                                            >  2:11687426‑M1/5‑137 (MQ=255)
                        tTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCtt                                                                                                         <  2:16678090‑M1/136‑1 (MQ=255)
                        tTTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCtt                                                                                                         <  2:1496882‑M1/136‑1 (MQ=255)
                            attaattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGccc                                                                                                     >  1:14301215/1‑137 (MQ=255)
                              taattTTCGACAATTAAGCATCGGCATAGTCTCCCTGCATAATATACTACTACAATTTGAGGAACTAAACCCCTATGCCGCGGCCGCGCGAATTCGATCTTGGTACCAGGAGGAAAAACATCTTTGCCCTTCCCCCt                                                                                                   <  2:8045877/137‑1 (MQ=255)
                              taattGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCt                                                                                                   <  1:15718607/137‑1 (MQ=255)
                                    ttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGc                                                                                             <  2:3569271/137‑1 (MQ=255)
                                    ttgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGc                                                                                             >  1:2507751/1‑137 (MQ=255)
                                     tgACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCa                                                                                            >  1:13972814/1‑137 (MQ=255)
                                        cAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCacca                                                                                         <  1:13610486/137‑1 (MQ=255)
                                         aaTTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCaccaa                                                                                        >  1:14615110/1‑137 (MQ=255)
                                         aaTTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCaccaa                                                                                        >  2:1633008/1‑137 (MQ=255)
                                             aaTCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCaccaaacca                                                                                    <  2:9658648/137‑1 (MQ=255)
                                               tcatcGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCAccaaaccaaa                                                                                  >  1:2224451/1‑137 (MQ=255)
                                                      catagtatatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAAc                                                                           >  1:11710502/1‑137 (MQ=255)
                                                      catagtatatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAAc                                                                           >  2:5276709/1‑137 (MQ=255)
                                                        tagtatatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACGTGCACCAAACCAAACCCCAACAg                                                                         >  1:17971475/1‑137 (MQ=255)
                                                         agtatatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGt                                                                        >  1:17314889/1‑137 (MQ=255)
                                                          gtatatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTg                                                                       <  1:10002053/137‑1 (MQ=255)
                                                            atatcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCCACATGCACCAAACCAAACCCCAACAGTGGc                                                                     >  1:3500335/1‑137 (MQ=255)
                                                               tcggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATg                                                                  <  1:5243158/137‑1 (MQ=255)
                                                                 ggcatagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGAc                                                                >  2:7735003/1‑137 (MQ=255)
                                                                   catagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGc                                                              >  2:3888093/1‑137 (MQ=255)
                                                                   catagtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGc                                                              >  2:14573371/1‑137 (MQ=255)
                                                                   catagtataATACGAAAAGGTGAGGAACTAAACCCCTAGGCCGCAACCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAAAATATGTGCCATTGCCCATACATGCACCAAACCAAACCCCAAAAGTGGCATGACga                                                              >  2:18837352/1‑136 (MQ=255)
                                                                      agtataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCaa                                                           >  1:12965387/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGACAAAc                                                         >  2:9402983/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCAAACAGTGGCATGACGCCAAAc                                                         >  2:14154792/1‑137 (MQ=255)
                                                                        tataATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCCTGCCCCTACATGCAACAAACCAAACCCCAACAGTGGCATGACGCCAAAc                                                         >  2:18494664/1‑137 (MQ=255)
                                                                         ataataCGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACt                                                        <  2:17367023/137‑1 (MQ=255)
                                                                                gACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTc                                                 >  2:6971123/1‑137 (MQ=255)
                                                                                gACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTc                                                 <  1:7919375/137‑1 (MQ=255)
                                                                                gACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTc                                                 >  1:11396888/1‑137 (MQ=255)
                                                                                gACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTc                                                 <  1:17644563/137‑1 (MQ=255)
                                                                                  cAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGc                                               >  1:3856139/1‑137 (MQ=255)
                                                                                     ggTGCGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAGACTGGACTTCGCCGa                                            >  2:12736049/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCt                                                                                                   >  2:15725294/1‑82 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:10381888/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  2:138036/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  2:3150309/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  2:18096820/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  2:6212086/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:1077585/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:1829382/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:3196726/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  2:10687528/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  2:10290738/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:6554624/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:9273228/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:6905354/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCAGa                                            >  1:11108802/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACCTCGCCGa                                            >  1:9914988/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCACCTACATGCAACAAACCAAACACCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  2:2485569/1‑137 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAAGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGAAGCCAAACTGGACTTCgaaga                                            >  2:8392482/1‑133 (MQ=255)
                                                                                     ggTGAGGAACTAAACCCATAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGa                                            >  1:13320880/1‑137 (MQ=255)
                                                                                       tGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGaaa                                          <  2:12371641/137‑1 (MQ=255)
                                                                                        gAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAAt                                         >  1:1902848/1‑137 (MQ=255)
                                                                                        gAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAAt                                         >  2:13096131/1‑137 (MQ=255)
                                                                                           gAACTAAACCCCTAAGCCGCGGCCGCGCGAATTCGAACTTGGTACCAGGAGGAAAAACATATGCGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGTAATGACGCACAACTGGACTTCGCCGAAACCAt                                      >  2:5922205/1‑137 (MQ=255)
                                                                                                  aCCCCTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCATGCATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTAc                               >  1:16388358/1‑137 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                             <  1:8246334/137‑1 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                             >  2:17763215/1‑137 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                             >  2:4917657/1‑137 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                             <  2:7321292/137‑1 (MQ=255)
                                                                                                    cccTAGGCCGCGGCCGCGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTACCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACgg                             <  2:17012584/137‑1 (MQ=255)
                                                                                                         ggccgcggccgcGCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGAtt                        >  1:16982010/1‑137 (MQ=255)
                                                                                                           ccgcggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTAc                      >  2:12051537/1‑137 (MQ=255)
                                                                                                            cgcggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTAcc                     >  2:2830939/1‑137 (MQ=255)
                                                                                                            cgcggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTAcc                     >  1:7272412/1‑137 (MQ=255)
                                                                                                             gcggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCt                    <  1:4917657/137‑1 (MQ=255)
                                                                                                             gcggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCt                    <  1:16628407/137‑1 (MQ=255)
                                                                                                              cggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTg                   <  1:8959012/137‑1 (MQ=255)
                                                                                                              cggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTg                   <  2:4623776/137‑1 (MQ=255)
                                                                                                              cggccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTg                   <  1:16075235/137‑1 (MQ=255)
                                                                                                                 ccgcnCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTNGCCGNAATCATGAGTTACGGCGATTACCTGGGa                >  2:9085739/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCt                                                                                                   <  1:9580673/54‑1 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                >  2:11051858/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                >  2:6609429/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                >  1:4532885/1‑137 (MQ=255)
                                                                                                                 ccgcgCGAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGa                >  1:8585508/1‑137 (MQ=255)
                                                                                                                     gcgAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTgg            <  2:11392281/137‑1 (MQ=255)
                                                                                                                       gAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGAt          >  1:5547096/1‑137 (MQ=255)
                                                                                                                       gAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGAt          >  2:8751779/1‑137 (MQ=255)
                                                                                                                       gAATTCGAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGAt          >  1:7596698/1‑137 (MQ=255)
                                                                                                                       gAATTCGAGCTTGGTACCAGGAGGAAAAAAATATGTGCCCTTGCCCCTACATACACCAAACCAAACCCCAACAGTGGCATGACGCAAAACTGGACTTCGCCGAAATCACGAGTTACGACGATTAACTGGGACTGGAt          >  2:15332958/1‑137 (MQ=255)
                                                                                                                             gAGCTTGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGATAAAGTg    <  1:11985326/137‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAATTAATTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCCTAGGCCGCGGCCGCGCGAATTCGAGCTCGGTACCAGGAGGAAAAACATATGTGCCCTTGCCCCTACATGCACCAAACCAAACCCCAACAGTGGCATGACGCCAAACTGGACTTCGCCGAAATCATGAGTTACGGCGATTACCTGGGACTGGATAAAGTGCT  >  pS4413_qbsFGH/1‑239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑