BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	2,241,305	0	T	G	28.8%	406.0 / 46.8	285	intergenic (+52/‑229)	gltX CDS (Glutamate‑‑tRNA ligase)/PP_t25 tRNA (tRNA‑Ala)	–/–
Reads supporting (aligned to +/- strand): ref base T (154/46); new base G (20/61); total (174/111)
Fisher's exact test for biased strand distribution p-value = 5.16e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 291 total aligned reads displayed.

TGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTG  >  AE015451_phaM1/2241171‑2241441

    ccAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTc                                                                                                                                     >  2:15952753/1‑137 (MQ=255)

     cAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGNCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCa                                                                                                                                    <  1:15552915/137‑1 (MQ=255)

     cAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAACGCAACGGTAAATTCa                                                                                                                                    >  1:5505576/1‑137 (MQ=255)

        gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAATGCAATGGTAAATTCAata                                                                                                                                 >  2:16305905/1‑137 (MQ=255)

         ctctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAatat                                                                                                                                <  2:15153968/137‑1 (MQ=255)

          tctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc                                                                                                                               <  2:3050664/137‑1 (MQ=255)

           ctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCATCTTTGCACTTAACCAGTGGGAGCGGGCGTGCCCGCGGCTGCGATGGGAAATTTAAGATCg                                                                                                                              >  2:6828487/1‑137 (MQ=255)

           ctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTTTCCCCGAAAGCAACGGGAAACTCaagaccg                                                                                                                              >  1:16021382/1‑132 (MQ=255)

               aCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGTCCGCGAATGTAATGGTCAATTCCATCTCGCAtt                                                                                                                          >  1:12092662/1‑137 (MQ=255)

               aCCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAACGCAATGGTAAATTCCATATCGCAtt                                                                                                                          >  2:12555440/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAANGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATNCAATGGTAAACTCAATATCGCATTCGCCGGc                                                                                                                   >  2:1496664/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGGTTGGGAAAGGTTTTTGGCTTCCATTGCCTGATTCGGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAGTGCAGTGGGAAGTTCAATATCGCATTCGCCGGc                                                                                                                   <  1:12165228/137‑1 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGTGTTCCCTCGAAGGCAAATGTAAATTCCATATTGCATTCGCCGGc                                                                                                                   >  1:8687013/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGTGTGTCCGCGAATGCAAGGGGAAATTCAAGACCGCCCTCGCCggt                                                                                                                   >  2:13937221/1‑136 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCTCTAAGGCAATAGTACATTCCCCATCGCCTTCTCCgtc                                                                                                                   >  1:1897733/1‑135 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGACTGCAACGGTAAATTCAATATCGCATTCGCCAGc                                                                                                                   >  2:11470304/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGc                                                                                                                   >  2:5194842/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGc                                                                                                                   <  2:14061421/137‑1 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGc                                                                                                                   <  2:16420176/137‑1 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGTCAACTCCCCATCGCACTCGCCGGc                                                                                                                   >  2:9405786/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGAAACGGTAAACTCAATATCGAATTCGCCGGc                                                                                                                   >  2:1476854/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAACGCAAGGGGCAAGTCAATATCGCATTCGCCGGc                                                                                                                   >  2:1431908/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGAGAATGTAATGGTAAATTCAATATCGCATTCGCCGGc                                                                                                                   >  2:16628856/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGAGTGCCCGCGAATGCAATGGTAAATTCAACACCGCATTCGCCGGc                                                                                                                   >  2:2345469/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGAGTGCCCGAGAATGCAACGGTAAACTCCACATCACACTCTCCGGc                                                                                                                   >  1:13367498/1‑137 (MQ=255)

                      gggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCATGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGTCCACAAGGGTAATGGTCAACTCAATACCGAATTCGCCGGc                                                                                                                   >  1:9462112/1‑137 (MQ=255)

                      gggtggggtgGCGAAGAAAGAAAACAAAGAGGGGGAAAAGCTGTTGGCTTCCATCGCCGGGTTCAGCTTTGCACCTAACCAGGGGGAGCGGGCGTGCCCGCGAGTGCAGTGGGAAATTCAATATCGCATTCGCCGGc                                                                                                                   <  1:15011136/137‑1 (MQ=255)

                      gggtggggggTCGAAGAAAGAAAACAAGGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGc                                                                                                                   <  2:4547008/137‑1 (MQ=255)

                       ggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGTGTGCCCTCGAATGAAATGGTAAATTCGATATCGAATTCGACggaa                                                                                                                  >  1:2524542/1‑135 (MQ=255)

                       ggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCGCGAAGGCAACGGTAAATTCCACATCGCACTCACCAGCa                                                                                                                  >  2:10601532/1‑137 (MQ=255)

                       ggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCGCGAACGCAACGGTCAACTCCACATCGCACTCGCCCGCa                                                                                                                  >  2:13082539/1‑137 (MQ=255)

                       ggtggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAGGGTAAACTCCACACCGCACTCGCCAGCa                                                                                                                  >  2:7746936/1‑137 (MQ=255)

                           gggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCACGAAGGCAAGGGTAAACTCAATATCTCACTCGCCGGAACGcc                                                                                                              >  1:16596886/1‑137 (MQ=255)

                           gggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGTAATGGTAAATTCAACCTCGCATTCGCCGGCCCGcc                                                                                                              >  1:11318064/1‑137 (MQ=255)

                            ggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGACTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAAGGCAATGGTCAATTCAATATCGTATTCGCCGGCACGccc                                                                                                             >  2:4336631/1‑137 (MQ=255)

                              tgtCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAATGCAACGGTAAATTCAACATCGCACTCGCCGGCACGCCCGc                                                                                                           >  2:7449675/1‑137 (MQ=255)

                                tCGAAGAAAGAAAACAAAGATTGGAAAAATCTGTTGGCTTCCATCACCTGATTCAGCTGTGCACCGAACCAGTGGGAGCGGGCGTGCCCGCGAGTGCAGTGGGAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑c                                                                                                        <  2:9670483/137‑1 (MQ=255)

                                tCGAAGAAAGAAAAAAAGGGGTGGAAAAAGCTGTTGTCTTCCATTGCTTGATTCAGCTTGGCACCTAACCGGTGGGAGTGGGCGTGCCCGCGAATGAAGTGGGAAGTTCAATATCGCATTCGCCGGCACGCCCGCT‑c                                                                                                        <  2:9844033/137‑1 (MQ=255)

                                ccGAAGAACGAAAACAAAGAGTGGGAAACTCTTTTGGCTTCCATCCCCTTATCCAGCTTGGCACTTAACCAGTGAGAGCGTGCGTGCCCTCGAGTGAAATGGGAAAGTCACCATCGCCTTCGCCGGCACGCCCGCT‑c                                                                                                        <  2:11114051/136‑1 (MQ=255)

                                 cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGTCCGCGGAGGCAATAGGAAATTTAAGATCGCATTCGCCGGCAAACCCGCT‑cc                                                                                                       >  2:261966/1‑137 (MQ=255)

                                 cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAACTCAATATCGCCTTCGCCGGCACACCCGCT‑cc                                                                                                       >  2:690355/1‑137 (MQ=255)

                                 cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGAGTGCCCGCGAATGCAATGGTAAATTCAATATCGAATTCACCAGCACGCCCGCT‑cc                                                                                                       >  2:11238784/1‑137 (MQ=255)

                                 cGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGAGTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCGCGAAGGCAATGGTAAACTCCACATCGCACTCGCCAGCACACCCGCT‑cc                                                                                                       >  1:14196593/1‑137 (MQ=255)

                                    agaaagaaAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCACGAATGCAATGGTAAATTCCACACCGCATTCGCCGGCACACCCGCT‑ccccc                                                                                                    >  2:1895059/1‑135 (MQ=255)

                                     gacagaaaACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTAATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCATGCCCTCGAATGCACTGGTGGATTCAAAATCTACTTTACCGGCCCGCCCACT‑CCcaca                                                                                                   >  2:11211724/4‑137 (MQ=255)

                                     gaaagaaaACAAAGATTGGGAAAAGTTTTTGTCTTCCCTCGCCTGATTCAGTTTTGCACCGAACCGTTGGGAGTGGGTGTGCCTGCGAATGCAGTGGGAAATTCACTATTGCCTTCGCCGGCACGCCCGCT‑CCcaca                                                                                                   <  2:13152801/137‑1 (MQ=255)

                                     gaaagaaaACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCAAATGCAATGGTAAACTCAATATCGCATTCGCCGGCACGCCCACT‑CCcaca                                                                                                   >  2:10504112/1‑137 (MQ=255)

                                      aaagaaaACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTTCCCGCGAACGCAATGGTAAATTCAACACCGCATTCACCAGCACGCCCACT‑CCcacaa                                                                                                  >  2:6022299/1‑136 (MQ=255)

                                         gaaaACAAAGATTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCGTTGGGAGCGGGCGTGCCCGCGAATGCAGTGGTAAATTCACCATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGcc                                                                                               <  2:7591607/137‑1 (MQ=255)

                                          aaaaCAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGTAAATTCAACATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCa                                                                                              >  2:2051733/1‑137 (MQ=255)

                                             aCAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGAAATGGTAAATTCCACCTCGCATTCGCCGGCACGCCCCCT‑CCCACAGGCCAACt                                                                                           >  2:15499590/1‑137 (MQ=255)

                                                aaGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGAGTGCCCGCGAAGGCAATGGGAAACTCAACATCGCACTCGCCAGAACGCCCGCT‑CCCAAAGGACAAATAAg                                                                                        >  1:10671399/1‑137 (MQ=255)

                                                        gAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGAGTGCCCACGAACGAAATGGTGAAGGCAATACCCCACTCACCCGCACACCCTAC‑CCCCCAGAACAACCAAAACCCaaaa                                                                                >  1:8482521/1‑137 (MQ=255)

                                                          aaaGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTCAATTCAATATCACATTCGCCGGCACGCCCACT‑CCCACAAGCCAACTAAGCCCCaaaaaa                                                                              >  2:7262676/1‑137 (MQ=255)

                                                              cTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGTGTGCCCGCTAAGGAAATGGTAAATTCCACATCGCACTCGCCAGCACGCCCACT‑CCCCCAGGCCAACTAAGACCCAAAAAAACgg                                                                          >  1:15283143/1‑137 (MQ=255)

                                                              cTGTTGGCTTATATCTCCTGATTCAGTTTTGCGCCTAACCATTGGGGGCGGGTGTGCCCGGGACTGCGATGGGAAATCCACTACCGCATTTGCCGGAACGCCCGCT‑CCCACAGGCTAACTAAGCCCCAAAAAACCgg                                                                          <  2:9487021/137‑1 (MQ=255)

                                                              cTGTTGGCTCCCGTTGCCTGATTCAGCTTTGCACCCACCCAGTGGGAGGGGGCGGGCCGGCGAAGGAAGGGGGAAATTCAATATCGCCTTCGCAGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCgg                                                                          <  2:5887144/137‑1 (MQ=255)

                                                                  tgttttCCGTCGCCTGATCCAGCTTTGCACCGAACCAGGTGGAGCGGGCGTGCCCGCGAATGCAATGGGAAATCCAATCTCGCATTCCCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTa                                                                      <  2:16233364/133‑1 (MQ=255)

                                                                    gCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGTCCTCGAATGCCATGGTAAACTTCATACCGCATTCACCGGCACACCCGCT‑CCCACAGGACAACTCAGCCCCAAAAAACCTGGGTAgg                                                                    >  1:16846665/1‑137 (MQ=255)

                                                                    gCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGAGAACGGAAGGGGAAAGTCAAAGCCGCACTCGCCAGAACACCCGCT‑CCCACAAGACAAACAAGACCCAAAAAAACAGGGTggg                                                                    >  2:1295440/1‑137 (MQ=255)

                                                                      ttCCATCGCTTGATTCATTTTTACACCCAACCGGTGGGGGTGGGTGTGCCCGCGAGTGAAGGGGGAACTTCAATATCCCCTTCGCAGACACCCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGc                                                                  <  1:8392067/137‑1 (MQ=255)

                                                                      tccaaTCTCCGGATTCGTCTTGACACCTAACGGGGGGGGGGGGGGGTGCCAGAGAATGAAGGGGAAACTCCAATACCCCCTCCCCCGAAACCCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGc                                                                  <  1:5371662/133‑1 (MQ=255)

                                                                       tCCATCGCCTGATTCAGCTTGGCACCGAACCAGTGGGAGCGGGTGTGCCCGCGAGTGCAGTGGGAAATTCAGTATCGCATTCGCCGGCACTCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCg                                                                 <  2:8323906/137‑1 (MQ=255)

                                                                       tCCATCGCCGGATTCAGTTTTGCACCGAACCGGGGGGAGCGGGTGTGCCCGCGAGTGAAGGGGGAAATTCAATATCGCATTCGCTGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCg                                                                 <  2:15721765/137‑1 (MQ=255)

                                                                       tCCATCGCCGGATTCAGCTTTGCACCGAACCATTGGGAGAGGGCGTGCCCGCGAATGAAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCC‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCg                                                                 <  1:15359398/137‑1 (MQ=255)

                                                                         cATCGCCTGGTTAGGTTTTGCACCGAACCGGGGGGAGGGGGTGTGCCAGAGAGGGAAGGGGGAAATCAAATATCACCTTCGCCGAAACACCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGt                                                               <  1:6035301/137‑1 (MQ=255)

                                                                         cATCGCCCGATTCAGCTTGACACCTAACACTTGGGAGCGGGTGTGCCCGCTAATACAATGGTAACTTCAATATCGCGTTCGCCGACACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGt                                                               <  2:3597192/137‑1 (MQ=255)

                                                                          aTCGCCTGATTCAGTTTTGCACTGAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCACTATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTa                                                              <  2:4015610/137‑1 (MQ=255)

                                                                              ccTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGTCCACGAATGCAACGGTAAACTCCACACCGCACTCGCCAGCACACCCACT‑CCCCCAAGCCAAACAAAACCCAAAAAAACAGGGTAGGGAGGTAAATg                                                          >  2:4064509/1‑137 (MQ=255)

                                                                                 gATTCAGCTTTGCACCTAACCAGTGGGAGCGGGTGTGCCCGCGAATGAAAGGGTAAATTCAATCTCGCATTCTCCAGCACACCCGCT‑CCCACAGGCCAAATAAGACCCAAAAAACCAGGGTAGGGAGGTGAGTGGtt                                                       >  1:3216411/1‑137 (MQ=255)

                                                                                 gATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGTCCGCTAATGGAACGGTGAACTCAATATCGCATTCTCCGGCACGCCCACT‑CCCACAGGCCAAATAAACCCCAAAAAACCGGGGTAGGGAGGTAAATGagt                                                       >  2:462996/1‑135 (MQ=255)

                                                                                 gATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGCCCGCGAATGAAATGGTAAATTCCATATTGCATTCTCCGGCACGACCGCT‑CCCACAGGACAACTCAGCCCCCAAAAACCGGGGTTGGGGGGTGAGTGGtt                                                       >  1:12457966/1‑137 (MQ=255)

                                                                                     cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTGAAGTCAACATTGCGCTCTTCGGAACGCCCTCT‑CCCCCAGGCCAACCACACCCCCAAAAACCTGGGTAGGGTGGTAAGTGGTTGTTa                                                   >  2:14429340/1‑137 (MQ=255)

                                                                                     cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCCATATCGCATTCGCCGGCACGCCCACT‑CCCCCAGGCCAACTAAGCCCCAAAAAAACGGGGTCGGGCGGTAAGTGgttgttc                                                   >  2:3088304/1‑136 (MQ=255)

                                                                                     cAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCCCTCACCGGCACGCCCTCT‑CCCCCAAGCCAACTAAGCCCCAAAAAACCAGGGTAGGGCGGTAAGTGGTTGTTa                                                   >  1:7135819/1‑137 (MQ=255)

                                                                                           tGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATCTCACATTCGCCGGCACGCCCGCT‑CCCACAGGCAAACTAAGACCCAAAAAAACGGGGTGGGGCGGTAAGTGGTTGTTGCCtgtg                                             >  2:11865430/1‑137 (MQ=255)

                                                                                           tGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGTCAATTCCACACCTCACTCGCCCGCACACCCTCTCCCCCCAGCCCAACAAACCCCCAAAAAA‑CAGGGTGGGGGGGTAAGTGAGTGTTCTCtgtg                                             >  1:2950048/1‑137 (MQ=255)

                                                                                                cTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGTAAATTCCCTCTCGCATTCTCCAGCACGCCCCCT‑CCCACAGGCCAACTAAGACCCAAAAAACCAGGGTAGGGCGGTAAGTGATTGTTGTCTGTGaaaaa                                        >  2:3780292/1‑137 (MQ=255)

                                                                                                cTAACCAGTGGGAGCGGGCGTGCCCGCAAAGGCAATGGTAAATTCCACACCACATTCACCAGAACGGCCTCC‑CCCACAGGTCAACTAAGCCCCAAAAAACCGGGGGAGGGAGGTAAATGATTGTTCTCtgtggagaa                                        >  2:5403774/1‑132 (MQ=255)

                                                                                                     cAGTGGGAGCGGGCGTGTCCGCGAATTCCACTGTGAACGCAACACCGCAGTCGCCAGCACGCCCGCT‑CCCACAGGCAAAATAAGCCCCAAAAAAACGGGGTAAGGAGGTGAGTGAGTGTTATCTGTGGAAAAAtttt                                   >  2:804867/1‑137 (MQ=255)

                                                                                                     cAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCACTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAAACGGGGTAGGGCGGTAAGTGGTTGTTATCTGTGAAAAAAgttt                                   >  2:11161519/1‑137 (MQ=255)

                                                                                                     cAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAAGATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGAACCAAAAAAAAGGGGTGGGGCGGTAAGTGGTTGTTATCTGTGAAAAAAtttt                                   >  2:10076533/1‑137 (MQ=255)

                                                                                                     cAGTGGGAGCGGGCGTGCCCGCGAATGCAACGGTAAATTCCACATCGCCTTCTCCGGCACGCCCACC‑CCCACAGGCCAACTCAGCCCAAAAAAAACGGGGTAGGGTGGTAGGTGGTTGTTCTCTGTGAAAAAActtt                                   >  2:634127/1‑137 (MQ=255)

                                                                                                           gAGCGGGGGTGTCCTCGAATGCAAGGGTAAACTCAACATCGCATTCGCCGGCACGCCCGCT‑CCCACAAGACAACCCAACCCCAAAAAAACAGGGTGGGGGGGTAAGTGAGTGTTGTCTTTTAAAAAAttttttcatc                             >  2:6451461/1‑132 (MQ=255)

                                                                                                           gAGCGGGCGTGTCCGCGAATGCAATGGTAAATTCCATATCGCATTCGCCGGCACACCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCAGGGTAGGGCGGTGAGTGAGTGTTATCTGTGGAAAAATTTTTGAata                             >  2:13881899/1‑137 (MQ=255)

                                                                                                           gAGCGGGCGTGCCCGCGAATGCAAGGGTAAATTCCACATCGCATTCGCCGGCACGCCCACT‑CCCCCAGGCCAACCCAAACCCAAAAAAACGGGGTAGGGGGGTAAGTGAGTGTTATCTGTGAAAAAACTTTTGaacg                             >  1:10380260/1‑135 (MQ=255)

                                                                                                             gCGGGGTTGCCCGCGGATGTAATGGGAAAGTCAACATCGCATTCGCCGGCACGCCCGCT‑CCACCAGGACCACTAAGCCCCAAAAAAACGGGGTGGGGGGGTAGGTGGTTGGTATCCTTTAAAAAACTTTTGGATAtt                           >  2:10434887/1‑137 (MQ=255)

                                                                                                             gCGGGCATGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCAGCACGCCCGCT‑CCCACAGGACAACTCAGCCCCAAAAAAACGGGGTAGGGGGGTCAGTGGTTGTTGTCTGTGAAAAAATTTTTGAATAtt                           >  2:12197110/1‑137 (MQ=255)

                                                                                                                ggCGTGCCCACGAATGGAAGGGGAAATTCAATACCGCATTCGCCGGCACGCCCACT‑CCCACAGGCCAACTAAACCCCAAAAAAACGGGGTCGGGCGGTCAGTGATTGTTACCTGTGAAAAAATTTTTGAATCctttt                        >  2:13234944/1‑137 (MQ=255)

                                                                                                                  ggTGCCCGCGAAGGCAAGGGTAAAGTCCACATCGCACTCGCCGGCACGCCCGCT‑CCCACAGGACAACTCAACCCCCAAAAAAAAGGGTAGGGGGGTGCGTGGTTGTGATCTTTGAAAAAACTTTTTGATACTTTTGa                      >  2:15678835/2‑137 (MQ=255)

                                                                                                                         gcgAATGCAGGGGTAAATCCCCTCTCCCCTTCTCCGCCCCCCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAg               <  1:15902196/137‑1 (MQ=255)

                                                                                                                           aaaTGAAGTGGGAAGTTCAACCTCACATTTACTGGCACTCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGtt             <  1:11742594/136‑1 (MQ=255)

                                                                                                                             ctGCAGTGGTAAATCCGATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTg           <  2:892640/136‑1 (MQ=255)

                                                                                                                              tGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa          >  1:9287041/1‑137 (MQ=255)

                                                                                                                                 aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa          <  2:9081826/134‑1 (MQ=255)

                                                                                                                                 aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAg       >  2:1140755/1‑137 (MQ=255)

                                                                                                                                  aTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGc      >  1:7387553/1‑137 (MQ=255)

                                                                                                                                   tGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc     <  2:5263735/137‑1 (MQ=255)

                                                                                                                                      tAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTg  >  1:14689993/1‑137 (MQ=255)

                                                                                                                                      tAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTg  >  2:14621750/1‑137 (MQ=255)

TGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCT‑CCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTG  >  AE015451_phaM1/2241171‑2241441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`