Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,344,3080GA27.8% 110.1 / 14.3 104intergenic (+39/‑117)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
Reads supporting (aligned to +/- strand):  ref base G (49/16);  new base A (25/0);  total (88/16)
Fisher's exact test for biased strand distribution p-value = 4.47e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

GGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344174‑4344443
                                                                                                                                       |                                                                                                                                       
ggCGACAGCGTGCAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCCTTAACGCTCTTGTACGTTGCAGC‑GCCTGTCCGAGTGAGTAccgac                                                                                                                                       >  2:7906090/1‑135 (MQ=255)
ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCCAATCGAACAccgcc                                                                                                                                       >  2:16179779/1‑137 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGTGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:8399714/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAGCGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:14572478/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTTTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:14064689/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:2543760/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:8167450/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:6536440/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:6761279/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:13560354/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:15338019/137‑1 (MQ=255)
 gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTCATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:10500711/137‑1 (MQ=255)
  cGACAGCGTGACTGTGCGTTTTGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCGGAGCAAGTCGGCCGGGGCGCATTAACGCCCTTGTACGTTGCAGC‑GCCTGTCAGATCGAGCAccgcccg                                                                                                                                     <  2:102996/137‑1 (MQ=255)
  cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  1:2862278/137‑1 (MQ=255)
  cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  2:103035/137‑1 (MQ=255)
  cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  2:12405634/137‑1 (MQ=255)
  cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  2:1755633/137‑1 (MQ=255)
   gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGT‑GCCTTCCAAACCGAGCAcctcccgc                                                                                                                                    >  2:3504573/1‑137 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  2:5704930/137‑1 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  2:6189223/137‑1 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:560559/137‑1 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  2:9290578/137‑1 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  2:13458372/137‑1 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:12856614/137‑1 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:13268882/137‑1 (MQ=255)
      aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:15701462/137‑1 (MQ=255)
       gCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCgg                                                                                                                                <  2:13249262/137‑1 (MQ=255)
        cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                               <  1:14620708/137‑1 (MQ=255)
          tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGGCCGAGCACCACCCGCGCGGcac                                                                                                                             >  2:11612435/1‑137 (MQ=255)
          tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                             <  1:15489644/137‑1 (MQ=255)
             aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAGATCGAGCACCGCCCACGCGGCacaca                                                                                                                          >  2:4597151/1‑136 (MQ=255)
              ggTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACCGCCAGATCGAACCCCACCCACGCGGAACAAGa                                                                                                                         >  2:9544218/1‑137 (MQ=255)
              ggTGCGTTGGACAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCCGATTGAGCACCGCCCGCGCGGAACACGa                                                                                                                         >  2:8188591/1‑137 (MQ=255)
              ggTGCGTTGGACAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGTGGACCACGa                                                                                                                         >  2:8188590/1‑137 (MQ=255)
                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTATGCCCGATTGAGCACCACCCACGCAGAACACGACCTGGCa                                                                                                                  >  1:8730356/1‑137 (MQ=255)
                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAAATCGAACACCACCCACTCAGCACACGATcctgcc                                                                                                                  >  1:14790331/1‑132 (MQ=255)
                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAACACCCCCCACGCGGAACACGATTTGGCa                                                                                                                  >  2:2814960/1‑137 (MQ=255)
                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCCCCCAACCGAACACCACCCACGCAGCACACAATccgtca                                                                                                                  >  1:8628076/1‑132 (MQ=255)
                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTTCCCGATCGAAGACCTCCCACGCCGCACACGAGCTggag                                                                                                                  >  1:7577874/1‑135 (MQ=255)
                     tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTGCCCGATCGAACACCGCCCGCGTGGCACACGGTCTGGCa                                                                                                                  >  2:15574061/1‑137 (MQ=255)
                      gggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCGTGGCCGACCGAGGACCGCCAGCGCGGCAAACAAGCTGGccg                                                                                                                 >  1:11131399/1‑135 (MQ=255)
                       ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAga                                                                                                                >  1:10129472/1‑136 (MQ=255)
                         cAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTTTCCAG‑CCCAGAACCCCCCGCGCAGCACAAAACCTGGCaagag                                                                                                              >  1:14180040/1‑133 (MQ=255)
                          aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCCGATCGCGCCCCTCCCACGCGGCACACGATCTTGCAAgcac                                                                                                             >  2:5836682/1‑135 (MQ=255)
                            tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGCCAGGTTGAGTACAGCCCGCGCGGCACACAGGCTGGCAGGGGCTg                                                                                                           >  2:14836350/1‑137 (MQ=255)
                            tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAGATCGAACACCGCCCACGCGGGACACGATTTGGCAAGAGCTg                                                                                                           >  2:6621457/1‑137 (MQ=255)
                            tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGACCGCGCACCGCCCGCGCGGCACACGATCTGGTCGGAGCTg                                                                                                           >  2:14836394/1‑137 (MQ=255)
                             ggCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCACCCACGAGGAACACAATCTTGCCGGAGTTGc                                                                                                          >  2:14072121/1‑137 (MQ=255)
                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGTGAACTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGT‑GCCTTCCAAATCGAGTACCGGCCGTGCGGGACACGATCTTGCAGGTGCTgtaa                                                                                                        >  2:6087407/1‑134 (MQ=255)
                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGTCAGATCGAGCACCGCCCGCGCGGCACACGGGCTGGGAGGGGCTGCAc                                                                                                        >  2:6087408/1‑137 (MQ=255)
                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGTCCGAGCGAACACCACCCACGCTGCACACAACCTGGCAAGAGATGCAc                                                                                                        >  1:6297596/1‑137 (MQ=255)
                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGTCCAACCTAGCACCCCCCACGCAGCACACCATCTTGCAGGCGCTGTAc                                                                                                        >  2:14068998/1‑137 (MQ=255)
                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCCGGTCGAGCACCGCCCACTCGGGACAAGATCTGGCAGGAGATGCAc                                                                                                        >  2:1262987/1‑137 (MQ=255)
                               cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCCCTTGTACGTTGCAGC‑GACTGCCAGACCTAGCACCGCCCGCGCGGAACACAATCTGGCAGGCGCTgaca                                                                                                        >  2:16803605/1‑134 (MQ=255)
                                     cTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAAATCGAGAACCACCCGCGCCGGACACAACCTGGCAAGCGCTGCCCTAtttg                                                                                                  >  2:6560936/1‑135 (MQ=255)
                                         cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGTCAGGTCAAGGACCGCCAGCGCGGAAAAAGGTATGGAAAGCGCTGGACCCTTGggggg                                                                                              >  1:11966958/1‑136 (MQ=255)
                                         cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGACCTGGCAGGCGCTGCACTATTGGAGGc                                                                                              <  1:2955175/137‑1 (MQ=255)
                                           tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCCAACCCAACACCACCCACACAGCACACAACCCGGCAAGCGCCGCACCCTTTGAGGCGt                                                                                            >  1:10817288/1‑137 (MQ=255)
                                           tttATACCGAATGGGCCGAGAGCGACCTGAACAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑ACCTGCCAGATCGAGCACCACACGCGCGGCACACGATCTGGGATGCGCCGCACTATTGGAggagg                                                                                            >  2:15294279/1‑134 (MQ=255)
                                                 ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCGATCGAGAACCACCCACGCGGGACACAATCTGGAAGGGGCGGGACTAGTGGGGGAGTGCGccc                                                                                      >  1:9390763/1‑137 (MQ=255)
                                                 ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAGATCGAGCACCACCCACGCGGCACACGATCTGGCAGGCGCTGCACCATTGGAGGAGTACGccc                                                                                      >  2:10647423/1‑137 (MQ=255)
                                                     aTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAAACCGAACACCGCCCCCACGGCAAACGATCTTGCAAGCGCTGCACCATTGGGAGCATACACCCCATc                                                                                  >  2:16346367/1‑137 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGT‑GGATGTCAGATCGGGCAACCCCCCCCCTGGACACAACCTGGTAAGAGGTGCCCCACTTGGGGGGTACTCCCCATcac                                                                                >  1:15872457/1‑137 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCTCCCA‑ATCGAAAACCCCCCACACCGNACACAAACCCGCCAGCGATGCACTCCTTGAGGCATACAccccaccac                                                                                >  1:13653821/1‑132 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTCCCAGTCTAACACCGCCCGCGCAGCAAACAATCCTGTAGGCGATTCACTATTTGAGGCGTCCGCCCCATcac                                                                                >  2:191765/1‑137 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGTCAAAGCGAACACCCCCCACGCGGAACAAAAGCTTGCAGGAGCTGCCCTCTTTGCGGAGTCCTCCCCATcca                                                                                >  1:11790139/1‑135 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCTCCCGCGAGGGACACGATCTGGCAGGAAATGCCCTATTGGAGGCGTACGCCCCGTccc                                                                                >  2:7552434/1‑135 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGAACGAGCACCGCCCGCGCGGCAAACGACATGGCAGGCGCTGCACTCTTGGAGGCGTCCGCCCCGTcac                                                                                >  2:11819636/1‑137 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCGATCGAGCACCACCCACGCGGAACACGAGCCGGGAGGAGTTGGACTAGTGGAGGAATACGCCCCGTcaa                                                                                >  1:11790140/1‑136 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAGATCGAGCCCCGCCCGCGCGGCCCACGACCCGGCAGGTGCTGTACCACTGGAGGAGTACATCCCATcac                                                                                >  2:9076859/1‑137 (MQ=255)
                                                       gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAAATCGAAAACCACCCCCCCGGCCCAAAACCCGGTCGGCACTGCACCACTTGAGGAGTACACCCCCTcac                                                                                >  2:10148350/1‑137 (MQ=255)
                                                         gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAACACCGCCCGCGCGGTACAAGAGCTGGCAGGAGCTGCAATATTGGAGGCGTACGCCCCGTcacac                                                                              >  2:12193019/1‑136 (MQ=255)
                                                            gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCAATCGAGCACCCCCCCCGCGGCACACAAGCTGGCCGGCGCTGTACTCTTGGAAGCGTACGCCCCATcacactct                                                                           >  2:11203265/1‑133 (MQ=255)
                                                            gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTTCCAGATCGAGCACCGCCCACGCGGAGCAAGATCTGGAAGGGGCTGCACTAGTGGGGGCGTAAGCCCCGTCAAATTat                                                                           >  2:2821338/1‑137 (MQ=255)
                                                                      tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGT‑GCCCTCCAGAGCTAGCCCCGCCCCCGCTGCCCACGACCTGATAGGTGCTTCACCCTTGAGAGAGTCCACCCCGTCCCATTCTCCTCCCGCCa                                                                 >  2:15987439/1‑137 (MQ=255)
                                                                      tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGTCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCa                                                                 >  2:12809538/1‑137 (MQ=255)
                                                                      tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCACCCACACGGCACACGAGCTGGCAGGAGCTGCACCATTTGAGGCGTACGCCCCATCACACTATCCCCACGCCa                                                                 >  2:3607822/1‑137 (MQ=255)
                                                                      tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCGATCGAGCACCCCCCACACGGCAAACGAGCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCATCACACTCTCCTCCCGccc                                                                 >  2:15319054/1‑136 (MQ=255)
                                                                           aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:9076212/68‑1 (MQ=255)
                                                                           aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 >  2:9076212/1‑68 (MQ=255)
                                                                             gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGTCAAATCGCGCCCCGCCCGCGTTGTACACGCTCTTGCCGGCGCTGTACTCTTTGAGGTGTACGCCCCCTCCCATTATCCTTCCGCCACtgttgt                                                          >  2:4207439/1‑137 (MQ=255)
                                                                                ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑TCCTGCCCGGTCGGGCACCCCCCCCGCGGCACACCATTTGGCAGACAATGACCCTCCGGATGCGTCCGCCCCTTCCAATTATCATCCCGCCACTGTCGTCAt                                                       >  2:10773913/1‑137 (MQ=255)
                                                                                ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAAATCGAGCACCACCCCCGCGGCACACAAGCTGGAAGGAGCTGTAACCTTTGAGGAGTACGACCCGCCCAACTATCATCCCACCCCCGTCTTaag                                                       >  1:15860184/1‑136 (MQ=255)
                                                                                     gggCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGCCAGATCGAGGACCGCCCACACGGTACACGATCTGGGAGGAGCTGTCCTACTTGCGGTGGCCGTCCCGTCCCATTTTCCTCACGCCCCTCTCGTACCCTGcc                                                  >  2:10903696/1‑137 (MQ=255)
                                                                                     gggCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAAATCGAGCACCACCCACGCGGCACACGAGCTTGCAGGCGCTGCACCATCGGAGGCATACGCCCCATCACACTGTCACCCCACCCCCCTCGTAATCTTcc                                                  >  2:15802279/1‑137 (MQ=255)
                                                                                     gggCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAGATCGAGCACCGCCCACGAAGCACACGAACTGGCAGGCGCCGCAACATTTGAGGCGTACGCCCCGCCACCCTATCCGCACACCACCGTCGTCATCTGcc                                                  >  2:8840115/1‑137 (MQ=255)
                                                                                     gggCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCTGGACACGATCTGGCCGGCGCTGTCCTATTTGAGGCGTACGCCCCGTCCCATTCTCCTCCCGCCACCGTCTTAATCTGcc                                                  >  2:8840077/1‑137 (MQ=255)
                                                                                     gggCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCACCCACGCGGCACACGATCTGGCAGGCGATGCACTATTTGAGGCGTACGCCCCATCCCATTATCATCACGCCACCGTCGTAATCTGcc                                                  >  2:15799050/1‑137 (MQ=255)
                                                                                     gggCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCGATCGAGCACCGCCCACGCAGAACAAGACCTTGCAGGAGCTGCACTACTGGAGGCGTCCACCCCGTCAAATTCTTCTCACGCCAACGTCGTAATCTTcc                                                  >  2:6229633/1‑137 (MQ=255)
                                                                                     gggCGCATGAACGCTTTTGTACGTGGCAGC‑TCTTGACGGGTCGAGCACCGCTTGTGCGGCACACGGTTGGGCGGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGcc                                                  <  2:11450979/137‑1 (MQ=255)
                                                                                                        tACGTTGCAGC‑GCCTGCCAGATCGAACACCACCCACGCAGCACACAACCCTGCAAGAGACGCACCCCTCGAGGCGTGCGCCCCATTCCACTCTCCCCACACCAACGTCGTAATCCGTCCGCCAACCCCCTGCcacaa                               >  2:1104395/1‑136 (MQ=255)
                                                                                                                aGC‑GCCTTCCAGATCGAGCACCGCCCGCGCGGCACAAGATCTGGCAGGCGCTGCACTATTGGAGGAGTACACCCCATCAAATTATCCCCCCGCCACTGGTATAATCTTCCGGACAACTCTCTGTCACCGGTTCCCCg                       >  2:16528300/1‑137 (MQ=255)
                                                                                                                aGC‑GCCTGCCAGATAGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCg                       >  1:7199195/1‑137 (MQ=255)
                                                                                                                aGC‑GCCCGCCAGATCGAGCACCGCCCGCGCGGGACACGATCTGGTAGGCGCTGTCCTCTTTGAGGTGTACACCCCGTCACACTGTTATCCCACCACCGTCGTAATCTGTCGGCCAACTCTATGCCACCGGTCCCCCg                       >  2:3119643/1‑137 (MQ=255)
                                                                                                                           caaTCTAGCACCGCCCACGCGGCACACGATCTGGCAGGCGCTGTACCCTTGGAGGCGTACCCCCCTTTCCACTCTCACCCCTCCACTGTCGTCATCTGCCCGCCAACGCCCTGCCACAAGTCACCCAGCATGCTcct             >  2:14342484/3‑136 (MQ=255)
                                                                                                                           aaaTCGAGCACCGCCCACATGGCACACGACCTGGCAGGCGCTGTACTACTGGAGGCGTACGCCCCGTCAAAGTATCATCAAGACACTGGCGTAATCTGCCGGCCAACTCTCTGCAAAAGGGCACACGGTATGCTCCg             >  2:3176179/3‑137 (MQ=255)
                                                                                                                                gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  1:11644639/1‑137 (MQ=255)
                                                                                                                                    aCCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt    >  2:7846858/1‑137 (MQ=255)
                                                                                                                                      cgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt  >  1:14009613/1‑137 (MQ=255)
                                                                                                                                      cgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt  >  1:4990948/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344174‑4344443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑