BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,675,360	0	T	G	39.1%	208.1 / 47.6	193	intergenic (+59/+45)	PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)	–/–
Reads supporting (aligned to +/- strand): ref base T (67/45); new base G (0/72); total (67/126)
Fisher's exact test for biased strand distribution p-value = 3.09e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 194 total aligned reads displayed.

CGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAAT  >  AE015451_phaM1/4675226‑4675496

tggCCAGGACAATGCCTTTTAGACACCGGGCGAAGGGTTTGGTGTGTGTGTTGGGGTTTCCTACATCTTCTGCTGATGGGTGGGCGCGGGTTTACCCGCGAGTGTGTCGTTGAGTTTGCCGACGCGTTCGCGGGGaa                                                                                                                                             <  2:5549417/136‑1 (MQ=255)

cgtccAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACATCTTCCGCTGATGGGTGGGCGCGGTTTTACCCGCGAATTCGTCATTGAATTTGCCGACGCATTCGCGGGGaa                                                                                                                                             <  2:10153911/134‑1 (MQ=39)

cGGCCAGGACGATACCCGTTCGACACCGGGCGAAGGATTTGGCGTGGGTGCTGGGGTTTCTTACAGCTTCCTTTGATGGGTGGGTGTGGGTTGACCCGCGAGTGCGTTATGGAATTTGCCGACGCGTTCGTGGGGaa                                                                                                                                             <  2:14042310/137‑1 (MQ=255)

cGGCCAGGACAGTGCCCGTTCGACACCGGGTGAGGGATTTGGCGTGTATTCCGGGGTTCCCTCCAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGCCATTGAATTTGCCCACGCATTCGCGGGCaa                                                                                                                                             <  2:12312674/137‑1 (MQ=38)

cGGCCAGGACAGTCCCCGTTCGACACCGGGCGAAGGGTTTGGAGTGTATGCCGGGGTTTCCGACAGCTTCCGCGGACGGGTGGGCGCGGGTTTACCCGAGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTaa                                                                                                                                             <  2:14504686/137‑1 (MQ=38)

cGGCCAGGACAATTCCTGTTCAACACTGGGCGAAGGATTTGGCGTGCATGCCGGGGTTTCCTACATCTTCCGCTGAGGGGTGGGCGCGGGTTTACCCGCGAATGCGCCATGGAATTTGCCGACACATTCGCGGGGaa                                                                                                                                             <  2:11208190/137‑1 (MQ=37)

cGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCAGGTaa                                                                                                                                             <  1:12639960/137‑1 (MQ=255)

  gCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTTCCCCCAAACGCGTCCCTGAACTTTCCTACACACCCCCGGGTgaaa                                                                                                                                           >  2:15043361/1‑136 (MQ=255)

  aacAGGACAATGCCTGTTCGCCACCGGGCAAAGGATTTGGCGTTTATGCCGGGGTTTCCTACAGCTTCCGCTGATGGGTGGGCGTGGGTTTACCCGCGAAGGCTTCATTGAATTTGCCGACGCATTCGCGGGTAAAc                                                                                                                                           <  2:7383665/135‑1 (MQ=38)

   ccAGGACAATGCCCTTTAGACACCGGGCAAAGGATTTGGCGTGTGTGCCGGGGTTTCCTAAAGCTTCCGCTGACGGGGGGGAGTGGGTGTACCCGAGAATGCGTCATGGAATTTGCCGACGCATTCGCGGGGAAAcc                                                                                                                                          <  2:817438/137‑1 (MQ=255)

     aGGACAATGCCCGTTCGACACCGTGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGTTAAACCCg                                                                                                                                        <  1:1960687/137‑1 (MQ=255)

             tcccGTTCGACACCGGACCAAGGGTTTGTCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCGGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTaca                                                                                                                                <  2:14796384/136‑1 (MQ=38)

               ccGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTGCCCGCGAATACTTCCTTGTCTTTGCCGACGCATCCGCGGGTAAACCCACCCCTccacc                                                                                                                              >  1:16511851/1‑136 (MQ=255)

                ctttCAACACCGGGAGAAGGATTGGGCGTGTATGCCGGGGTTCCCGACATCTTCCACTGATGGTTGGGCGCGGGTTTACCCGCGAGTGCGTCATGAAATTTACCGACGCATTCGCGGGAAAACCCGCTCCTACACAg                                                                                                                             <  1:14052016/135‑1 (MQ=255)

                cGTTCGACACCGGGAGAAGGGTTTGGCGTGTATGCTGGGGTTTCCTACAGCTTCCGCTGACGGGGGGGCGCGGGTTTACCCGCGAGTGCGTCGTTGAGTTTGCCGACGCATTCGCGGGCAAACCCGCTCCTACACAg                                                                                                                             <  1:12491944/137‑1 (MQ=37)

                  ttCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCGGACGGGGGGGCGCGGGTTTACCCGCGAATGCGTCATGGAATTTGCCGACACGTTTGTGGGGAAACCCGCTCCTACACAggg                                                                                                                           <  1:14649739/137‑1 (MQ=38)

                     gACACCGGGCGAAGGATTGGGCGTGTATGCCGGGGTTTCCTACAGCTTCCCCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCGTTGAGTTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCg                                                                                                                        <  2:11309562/137‑1 (MQ=255)

                         ccGGGCGAAGGATTTGGCGTGGATTCCGGGGTTTCCTACAGCTTCCGCTGATGGGTGGGTGCGGGTTTACCCGCGAGTGCGTCATTGAGTTTTCCGACGCATTCGTGGGGAAACCCGCTCCTACACAGGGGCGTTGc                                                                                                                    <  1:7038387/137‑1 (MQ=255)

                          tgggTGAAGGATTTGGTGTGTGTGCTGGGGTTTCCTACACCTTCCGCTGACGGGGGGGTGCGGGTTTACCCGTGAATGTGTCGTGGAGTTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGca                                                                                                                   <  1:2781571/136‑1 (MQ=25)

                          cgtgCGAAGGATTTGGCGTTCAGTCCGGGGTTCCCTACACCTCCCGCTGACGGGTGGGCGCGGGTTCACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGca                                                                                                                   <  2:800523/134‑1 (MQ=25)

                          cGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCTCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCGTGGAATTTGCCGAAGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGca                                                                                                                   <  1:10316471/137‑1 (MQ=255)

                           gggCGAAGGGTTTGTTGTGTATTCCGGGGTTTCCTCCATCTTCTGCTGATGGGTGGGCGTGGGTTTACCCGCGAGTGCGTCGTGGAGTTTGCCGACGCGTTCGTGGGGAAACCCGCTCCTACACAGGGGCGTTGcac                                                                                                                  <  1:13003024/137‑1 (MQ=255)

                           gggCGAAGAATTTGGTGTGTAGGCCGGGGTGTCCTACAGCCTCCGCGGATGGGGGGGAGCGGGTTGACCAAAGAAGGTGCAATGAAATTTGCAGAAGAGTTCGGGGGGAAACCCGCTCCTACACAGGGGCGTTGcac                                                                                                                  <  2:2273908/137‑1 (MQ=255)

                              cGAAGGGTTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCGGACGGGTGGGCGTGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCAcagc                                                                                                               <  1:15905436/137‑1 (MQ=255)

                                  gggtttGGTGGGTATGCTGGGGTTTCCTACAGCTTCCGCGGATGGGTGGGCGCGGGTTGACCCGCGAGTGCTTCATTGAGTTTTCAGACACATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGa                                                                                                           <  1:6594089/134‑1 (MQ=255)

                                  gggtttGGCGTGTGTGCCGGGGTTTCCTACATCTTCCGCTGACGGGTGGGCGCGGGTTTACCTGCGAATGCGTCATGGAATTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGa                                                                                                           <  2:12085665/134‑1 (MQ=38)

                                  gggtttGGCGTGGGGGCTGGGTTTTCCGACATCTTCCGCGGATGGTTGGGCGGGGGTTTACCCGCGATTGCTTCATTGAGTTTTCCGACGCGTTCGGGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGa                                                                                                           <  1:1831830/134‑1 (MQ=255)

                                   gATTTGGCGTGTGTGCCGGGGTTCCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACACATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGAt                                                                                                          <  2:2012273/137‑1 (MQ=255)

                                    aTTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTGACCCAGAAGGCATCACCGGATTTGCCGACGCATTCGCGGGTGCAACCACTTCCCCACCGGGGCGTTGCAcaacagaagt                                                                                                         >  2:11141036/1‑133 (MQ=255)

                                       tGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACTGGTGGGCGTGGGTTTACCCGCGAATGCGTCGTTGAGTTTGCCTACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAgg                                                                                                      <  2:10698574/137‑1 (MQ=38)

                                            tgtATGCCGGGGTTTCCTACATCTTCCGCTGACGGGGGGGCGTGGGTTTACCCGCGAGTGTGTCGTGGAATTTGCCGACGCATTCGTGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTc                                                                                            <  1:7291246/137‑1 (MQ=38)

                                               aTGCCGGGGTTTCCTACATCTTCCTTTGACGGGTGGGTGCGGGTTTACCCACGAATTCGTCATTGAATTTGCCGACACATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGa                                                                                         <  2:1087804/137‑1 (MQ=255)

                                                tGCTGGGGTTTCCTACATCTTCCGTTGATGGGGGGGTGTGGGTTTACCCGAGAGTGCGTCGTTGACTTTGCCGACGCGTTCGTGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGAc                                                                                        <  1:4814239/137‑1 (MQ=255)

                                                tGCTGGGGTTCCCTACAGCTTCCGTTGACGGGTGGGAGCGGGTTTACCCGCGAATGCGTCGTGGAATTTGCCAACGCGTTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGAc                                                                                        <  1:14305529/137‑1 (MQ=37)

                                                tGCCGGGGTCTCATGCAACTTCCGCTGATGGGTGGGCGCGGTTTTACCCAAGAATACGCCATGGAATTTGCCGACACATTAACGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTcccac                                                                                        <  2:2950316/137‑5 (MQ=255)

                                                 gctgggtTTCCCTACAGCTCCCGCGGACGGGTGGGCGTGGTTTTACCCGAGAGTGCGTCGTTGAGTTTTCCGACGCGTTTGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGAcc                                                                                       <  1:5512075/134‑1 (MQ=255)

                                                 gCCGGGGTTTCCTACATCTTCCGCTGATGGGGGGGCGTGGGTTTACCAGAGAGGGCGTCATGGAATTTGCAGACGCGTTTGTGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGAcc                                                                                       <  1:11715723/137‑1 (MQ=255)

                                                 acgggggTTTCCTAAATTTTCCGTTGAGGGGGGGGCGGGGTTTTACCAGCGAGTGTTTCATGGATTTGTCCGACGCGTTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGAcc                                                                                       <  1:4392910/134‑1 (MQ=255)

                                                      ggTTTCCTACGTCTTCCGTTGACGGGTGGGCGTGGGTTTACCCGCGAATGCTTCATGGAATTTGCCGACGCGTTCGTGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCt                                                                                  <  1:5982661/137‑1 (MQ=255)

                                                      ggTTTCCTACATCTTCCGTGGGTGGGGGGGTGGGGTTTTCCCCGAGGGTGTGTCGTGGAGTTTGCCGACGCGTTCGTGGGAAACCCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCt                                                                                  <  1:255198/137‑1 (MQ=255)

                                                      ggTTTCCTACAGCTTCCGCGGATGGGGGGGCGTGGGTTTACCCGCGAATGCGTCATGGAATTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCt                                                                                  <  1:11585401/137‑1 (MQ=38)

                                                      ggTTTCCTACACCTTCCCCGAACGGGTGGGCGCGGGTTGACCCGCGAAGGCGTCATTGAATTTGCCGACGAATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCt                                                                                  <  2:2860465/137‑1 (MQ=255)

                                                      ggTTTCCGACGGCTTCCGCTGACGGGTGGGTGCGGGTTTACCTGAAAAGGTGTAATTGAATTTGCCGACGCATTCGTGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCt                                                                                  <  2:1711412/137‑1 (MQ=38)

                                                                  tttCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCGTGGAGTTTGCCGACGCATTCGGGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCAc                                                                      <  1:13121925/136‑1 (MQ=38)

                                                                  cTTCCGTTGATGGGTGTGCGTGGTTTTCCCCGCGAGTTCGTCATTGAGTTCGCCGACGCGTTTGTGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCAc                                                                      <  1:7409766/137‑1 (MQ=255)

                                                                  cTTCCGCTGATGGGTGGGCGCGGTTTTACCCGAGAATGCGTCATTGAGTTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCAAAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCAc                                                                      <  2:16235033/137‑1 (MQ=38)

                                                                  cTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCAc                                                                      <  2:13558372/137‑1 (MQ=255)

                                                                         tGATGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGAc                                                               <  1:6523121/137‑1 (MQ=255)

                                                                         tGATGGGGGGGCGCGGGTTTACCCGCGAATGCGTCGTGGAGTTTGCCGACGCATTCGTGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGAc                                                               <  2:5942442/137‑1 (MQ=38)

                                                                         ggACGGGTGGGCGCGGGTTGACCCGAGAAGGCGGAATTGAATGTGCCGACGCATTCGAGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGAc                                                               <  2:11607233/136‑1 (MQ=255)

                                                                            cGGGTGGGCGCGGGTTTACCCGCGAATGCGTCGTGGAGTTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCa                                                            <  2:3927714/137‑1 (MQ=255)

                                                                              cgcattcgcgGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCaaa                                                          >  1:15257434/7‑137 (MQ=255)

                                                                              cgcattcgcgGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCaaa                                                          >  1:5781194/7‑137 (MQ=255)

                                                                                     gcgGGTTGACCCGCGAATGCTTCATGGAATTTGCCGACGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCg                                                   <  2:7999666/137‑1 (MQ=255)

                                                                                         gTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGTGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACaa                                               <  2:15307683/137‑1 (MQ=255)

                                                                                           ttAACCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACCGTAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCCCCCATGACGCAAACCTGTCGACAAAc                                             >  1:9473064/1‑137 (MQ=255)

                                                                                                 gcaaaTGCTTCGTTGAGTTTGCCAACGCGTTCGCGGGCAAACCCGCCCCTACACAGGGGCGTTGCACAGCAGGATTAGGA‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGc                                       <  2:9773643/134‑1 (MQ=255)

                                                                                                  cggaTGCGTCATTGAATTTGCCGATGCATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCa                                      <  1:8617623/134‑1 (MQ=255)

                                                                                                     aTGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGa                                   >  2:9478786/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  2:11927663/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  1:3731864/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  1:3911440/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  2:15860402/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  1:788915/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  2:15083360/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  1:13959441/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  2:13709633/1‑137 (MQ=255)

                                                                                                      tGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                                  >  1:10246181/1‑137 (MQ=255)

                                                                                                       gtgTCATTGAATTTGCTGACGCGTTCGCGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAATTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAAc                                 <  2:3027319/135‑1 (MQ=255)

                                                                                                       gCGTCATTAAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAAc                                 <  2:6588994/137‑1 (MQ=255)

                                                                                                         gTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACgg                               >  2:13021505/1‑137 (MQ=255)

                                                                                                           cATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTc                             >  1:8106679/1‑137 (MQ=255)

                                                                                                           cATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTc                             <  1:15083360/137‑1 (MQ=255)

                                                                                                             ttGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           >  1:11018157/1‑137 (MQ=255)

                                                                                                             ttGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           >  1:5124564/1‑137 (MQ=255)

                                                                                                             ttGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           <  2:7266240/137‑1 (MQ=255)

                                                                                                             ttGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           <  1:16199265/137‑1 (MQ=255)

                                                                                                             ttGAATTTGCCGACGCATTCGAGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           <  2:3697735/137‑1 (MQ=255)

                                                                                                             ttGAATTTGCCGACACATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           <  2:731846/137‑1 (MQ=255)

                                                                                                             ttGAATTTCCCGACACATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           <  2:11835687/137‑1 (MQ=255)

                                                                                                             tggAATTGGCCGACACATTCGCGGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                           <  1:15372027/135‑1 (MQ=255)

                                                                                                              tGAGTTTGCCGACTCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgcg                          <  2:9331593/137‑1 (MQ=255)

                                                                                                                aaTTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCATGCAAGAACGGTCGCGTc                        >  2:6396667/1‑137 (MQ=255)

                                                                                                                     gCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTc                   >  2:16531726/1‑137 (MQ=255)

                                                                                                                     gCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTc                   >  2:15740132/1‑137 (MQ=255)

                                                                                                                        gACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt                >  1:1958699/1‑137 (MQ=255)

                                                                                                                        gACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt                >  1:3003083/1‑137 (MQ=255)

                                                                                                                        gACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt                >  2:5573031/1‑137 (MQ=255)

                                                                                                                        gACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt                >  2:16716806/1‑137 (MQ=255)

                                                                                                                        gACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt                >  1:9094202/1‑137 (MQ=255)

                                                                                                                        gACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt                >  1:13455209/1‑137 (MQ=255)

                                                                                                                         aCGCATTCGCGTGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTg               <  2:4839330/137‑1 (MQ=255)

                                                                                                                         aCGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTg               >  2:10880347/1‑137 (MQ=255)

                                                                                                                             aTTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt                >  2:10921865/1‑137 (MQ=255)

                                                                                                                             aTTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAgtt           >  2:2452150/1‑135 (MQ=255)

                                                                                                                             aTTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCt           >  2:14204065/1‑137 (MQ=255)

                                                                                                                             aTTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCt           >  1:10940145/1‑137 (MQ=255)

                                                                                                                             aTTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCt           >  1:14156082/1‑137 (MQ=255)

                                                                                                                                cgcgGGGAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGc        <  2:4929807/137‑1 (MQ=255)

                                                                                                                                     gTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGaa   <  2:6816096/137‑1 (MQ=255)

CGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTTTACCCGCGAATGCGTCATTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGG‑‑‑‑‑GTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAAT  >  AE015451_phaM1/4675226‑4675496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`