Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,222,9940GT57.1% ‑5.1 / 13.1 28L435M (CTG→ATG) PP_4603 CDS (transposase)
Reads supporting (aligned to +/- strand):  ref base G (1/11);  new base T (0/16);  total (1/27)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGC  >  AE015451_phaM1/5222912‑5223124
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gggagtgCTGGGTGATTTCTTATCAAACAAGCTGCGACCCATTGTTCTCTAAGCACTCACGCGGCCACCAGTTGATACCCCAGTTTCCTGGCTTTGCGAAACAAAGCCCGGACCTGCCGGTCTTTGCTTCTGGTTTc                                                                              <  2:2891880/131‑1 (MQ=1)
     ggttGGGTGATCTTTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCTGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAAct                                                                         <  1:1702106/134‑1 (MQ=0)
       cgggttGATCTCTCATCAAACAAAATGAAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAActcc                                                                       <  1:11859020/132‑1 (MQ=0)
                 tctccccaaaaaaaATGCACCCCATTTTTTTCGAAGCACTAAAGTGGCCACCATTGGAGACCCCATTTTCCGGGTTTTGCGAAGAAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                             <  1:1549291/131‑1 (MQ=1)
                 tcgcatcaAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGAGACCCCATTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                             <  2:13976992/134‑1 (MQ=0)
                 tcgcaccaaacaaaATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAACAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                             <  2:5811174/131‑1 (MQ=0)
                 tcccatcaAACAAAAGACAACCCATTTTTTCCTAAACACTAAAGCGGCCACAGGTTGGCACCCCATTTTCTGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                             <  1:9140338/134‑1 (MQ=1)
                 ccccaccaaacaaaAGACAACCCATGGTTTTCTAAGCACCCAAGTGGCCACCAGTTGATACCCCAGTTTCCGGGTTTGGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                             <  1:6039184/131‑1 (MQ=1)
                  cccaacaaaaaaaAAGAAAACCAGTTTTTTTCGAACCACTAAAGAGGCCACAGGTGGATACCCCGTTTTCTGGGTTTGGAAAAGAAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCtt                                                             <  1:689980/132‑1 (MQ=0)
                   tcctcaCACAAAATACAACCCATTGTTTTCTCAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGt                                                           >  2:9513128/4‑137 (MQ=0)
                             aagtGCAACCCGTTGTTTTCGAAGCACTCTAGAGCGGCCACCGTTTGATACCCCGTTTTCCGGGCTTTGAGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATa                                                   <  1:6577975/134‑1 (MQ=1)
                                tgaaaCCCATTTTTTTCTAAGCACCCAAGCGGCCACCAGTGAATACCCCAGTTTCCGGGCTTTGCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCt                                              <  1:7945393/134‑1 (MQ=1)
                                          tttttttCTAAGCACTCAAGCGGCCACCAGTTGATACCCCATTTTCCGGGTTTGGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTgg                                     <  1:15193842/134‑1 (MQ=0)
                                          tttttttCTAAGCACTCAAGCGGCCACCAGTTGATACCCAATTTTCTGGGCTTTGCAAAACAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTgg                                     <  1:4614453/134‑1 (MQ=1)
                                          tttttttCTAAGCACCCAAGCGGCCACCATTGGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAATCCTGCTTCTTgg                                     <  1:9323057/134‑1 (MQ=1)
                                          tttttttCGAAGCACTCAAGCGGCCACCAGTTGATACCCCATTTTCTGGGTTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTgg                                     <  2:14169810/134‑1 (MQ=1)
                                          tttttttCGAAGCACCAAAGCGGCCACCAGTGGATACCCCATTTTCCGGGCTTGGAGAAAAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTgg                                     <  1:4614499/134‑1 (MQ=1)
                                          tttttctCTAGGCACTCAAGCGGCCACCAGTTGAGACCCCATTTTCCGGGCTTTGAGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTgg                                     <  2:5890914/131‑1 (MQ=1)
                                               ttttAAGCACTCAAGCGGCCACCAGTTGATCCCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAAc                                <  2:1668616/134‑1 (MQ=1)
                                                tcgaaGCACTCAAGCGGCCACCAGTTGATACCCCGTTTTCTGGGTTTGGAGAAAAAAATCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                               <  1:4772237/134‑1 (MQ=1)
                                                tcgaaGCACCCAAGCGACCACAGGTTGACCCCCCGTTTTCTGGGCTTTGAGAAAAAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                               <  1:6457846/134‑1 (MQ=1)
                                                caaaaccACTCAAGCGGCCACAGTTGGATACCCCATTTTCCGGGCTGTGCGAGAAAAAACCCGGACCTGCCGGTCTTGGCTTCTGGTTGCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                               <  1:7331908/131‑1 (MQ=1)
                                                  gaaacACTCAATCGGCCACCAGTGGATACCCCAGTTTCCGGGCTTGGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAgg                             <  1:5172927/133‑1 (MQ=0)
                                                  gaaacACCCAAGCGGCCACCGGTGGAGCCCCCGTTTTCTGGTTTTGGAAAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAgg                             <  1:6096966/133‑1 (MQ=1)
                                                             aaccGGCCACCAGTTGATACCCCATTTTCTTGGTTTTGCGAAGCAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAATCCTGCTTCTTGGTTAACAGGTTGTACAcc                    <  1:1060420/134‑1 (MQ=1)
                                                                           tggacaCCCCGGTTTCGGGGCTTGAAAAAGAAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACt       <  1:12765822/132‑1 (MQ=1)
                                                                              atcccccATTTTCTGGGTTTTGAGAAGAAAATCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGAt    <  1:4556220/134‑1 (MQ=1)
                                                                                actccAGTCTCCGGGCTTTGCGAAGCAAAGCCCGGATCTACCGTTTTTGGCTTCTGGTTGCGAACTCCTCAGGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATgc  <  2:6902743/134‑1 (MQ=1)
                                                                                      |                                                                                                                                  
GGAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGC  >  AE015451_phaM1/5222912‑5223124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: