Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 1,713,060 A→G T2A (ACC→GCC)  tsaB CDS (endopeptidase) →

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM11,713,0600AG99.8% 2414.7 / ‑5.4 594T2A (ACC→GCC) tsaB CDS (endopeptidase)
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (347/246);  total (347/247)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 598 total aligned reads displayed.

ACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712929‑1713192
                                                                                                                                   |                                                                                                                                    
aCGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGgccacc                                                                                                                                 <  1:14825159/137‑1 (MQ=255)
 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTATGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  2:185905/137‑1 (MQ=255)
 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:1696895/137‑1 (MQ=255)
 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:13161643/137‑1 (MQ=255)
  gCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCct                                                                                                                               <  2:5593676/137‑1 (MQ=255)
      cTCGACAACGGCCCGTTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctg                                                                                                                           <  2:5736673/137‑1 (MQ=255)
      cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctgctt                                                                                                                           <  1:4168194/137‑2 (MQ=255)
      cTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTGGTAGGCCGCACAGCGCCCCCTCATGACACAACGCCGCTGCTGCTCTATACTGCCCCTCTTTTTCATTCGCACCGGGATACCCGTTCGATGGCCACCctgctg                                                                                                                           <  1:6957510/137‑1 (MQ=255)
       tCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTgg                                                                                                                          <  1:4898032/137‑1 (MQ=255)
               ggCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCCCAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATATTGCCGCTCTTTTTCCTTCGCCCCTTGATACCCGCTCGATGGCCAACCTCCTGGCCCTGGac                                                                                                                  >  2:3291348/1‑136 (MQ=255)
               ggCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGAt                                                                                                                  >  2:7543610/1‑137 (MQ=255)
                gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                 <  2:1385305/137‑1 (MQ=255)
                 cccGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATAc                                                                                                                >  1:15390366/1‑137 (MQ=255)
                   cGCTTTCGGGCCGTTGTCGTTTTTCGGCCGCACCGCGCCCCCTCCGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCCTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTTGCCCTGGATACCg                                                                                                              >  2:4208240/1‑137 (MQ=255)
                   cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCCCAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAACCTGCTGGCCCTGGATCCCg                                                                                                              >  2:6274667/1‑137 (MQ=255)
                   cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAACCTGCTGGCCCTGGATACCg                                                                                                              >  2:7164520/1‑137 (MQ=255)
                     cTTGCGGGCCGTTGTCGTTTTTAGGCCGCACAGCGCCCCCTCGTGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTCTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  2:14886443/137‑1 (MQ=255)
                     cTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGcc                                                                                                            <  1:11682150/137‑1 (MQ=255)
                      ttGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCa                                                                                                           >  2:1337469/1‑137 (MQ=255)
                           ggCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGaa                                                                                                      >  2:9549684/1‑137 (MQ=255)
                                   tCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTc                                                                                              >  1:7849794/1‑137 (MQ=255)
                                   tCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTc                                                                                              >  1:4113140/1‑137 (MQ=255)
                                   tCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTc                                                                                              >  2:3920162/1‑137 (MQ=255)
                                   tCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCATTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTc                                                                                              >  2:8680702/1‑137 (MQ=255)
                                     gTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCg                                                                                            <  2:10657383/137‑1 (MQ=255)
                                     gTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCg                                                                                            <  1:102337/137‑1 (MQ=255)
                                      tttGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGt                                                                                           <  2:3663471/137‑1 (MQ=255)
                                       ttGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTc                                                                                          <  1:13840143/137‑1 (MQ=255)
                                       ttGGAGGCTGCACAGCGCCCCCTTAGGACACAACGCCGCTGCTGCTCTATACTGCTGCTCGTTTTCATTCGCACCTGGATACCCGTTCGGTGGCCACCCTGCTGGCCCTGGATACCGCCACCTAAGCCTGTTCCGTc                                                                                          <  2:5425979/137‑1 (MQ=255)
                                           aGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCgcgc                                                                                      >  2:2060600/1‑137 (MQ=255)
                                           aGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCgcgc                                                                                      >  2:14215442/1‑137 (MQ=255)
                                               cGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctgct                                                                                  <  1:11254101/137‑1 (MQ=255)
                                                gCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                 >  1:9658906/1‑137 (MQ=255)
                                                gCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                 >  2:9824356/1‑137 (MQ=255)
                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:15641724/1‑137 (MQ=255)
                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:16428077/1‑137 (MQ=255)
                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:2417403/1‑137 (MQ=255)
                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             <  2:5960302/137‑1 (MQ=255)
                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAACGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:3120865/1‑137 (MQ=255)
                                                         ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTTGCACCTGGATACCCGTTTGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGACGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  2:15144449/1‑137 (MQ=255)
                                                              cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  1:16645671/137‑1 (MQ=255)
                                                              cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  1:16530485/137‑1 (MQ=255)
                                                              cAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   >  1:16116389/1‑137 (MQ=255)
                                                                 gACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAc                                                                <  2:16020733/137‑1 (MQ=255)
                                                                  acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:12388041/137‑1 (MQ=255)
                                                                  acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:865348/137‑1 (MQ=255)
                                                                  acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:15713243/137‑1 (MQ=255)
                                                                  acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:10352994/137‑1 (MQ=255)
                                                                   cacaACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGCTACCCGTTCGACGGCCACCCTGATGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCa                                                              >  2:12073660/1‑137 (MQ=255)
                                                                       aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  1:622410/137‑1 (MQ=255)
                                                                       aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  2:7894350/137‑1 (MQ=255)
                                                                        cgccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTcgcg                                                                                       <  2:12827789/107‑1 (MQ=255)
                                                                        cgccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCAc                                                         >  1:14461558/1‑137 (MQ=255)
                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  2:4468148/1‑137 (MQ=255)
                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  1:10760195/1‑137 (MQ=255)
                                                                           cgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTAc                                                      >  2:1174671/1‑137 (MQ=255)
                                                                            gctgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACg                                                     >  1:11928590/1‑137 (MQ=255)
                                                                                 tgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTg                                                >  2:9290670/1‑137 (MQ=255)
                                                                                 tgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTg                                                <  1:459490/137‑1 (MQ=255)
                                                                                           tGCCGCTCTTTTTCATTCGCACCTGGATACCCGCTCGATGGCCACCCTGCTGGCACTGGATACCACCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgca                                      >  2:8378556/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTgg                                                                                                                          >  2:2755805/1‑48 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCGTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:14194352/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               >  2:8901448/1‑107 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:8901448/107‑1 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:10046942/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:5796489/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:6492430/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:657600/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:3075268/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:5237876/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:1370025/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:16426718/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:2233762/1‑137 (MQ=255)
                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:16164357/1‑137 (MQ=255)
                                                                                                  ctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGGTCCCGCGCatgcatgc                               <  1:7310903/137‑1 (MQ=255)
                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:3297973/1‑137 (MQ=255)
                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:14572672/1‑137 (MQ=255)
                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:2191722/1‑137 (MQ=255)
                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:7035187/1‑137 (MQ=255)
                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:12781375/1‑137 (MQ=255)
                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                >  1:9332460/1‑37 (MQ=255)
                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  1:12651633/1‑137 (MQ=255)
                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:11258803/1‑137 (MQ=255)
                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:2617926/1‑137 (MQ=255)
                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  1:15018726/1‑137 (MQ=255)
                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACTAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  2:16684265/137‑1 (MQ=255)
                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  1:7564851/137‑1 (MQ=255)
                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  2:2877411/137‑1 (MQ=255)
                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  1:192415/137‑1 (MQ=255)
                                                                                                               aCCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGctgctg                  <  1:8496505/137‑1 (MQ=255)
                                                                                                                    gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  1:2487792/137‑1 (MQ=255)
                                                                                                                    gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  1:9178667/137‑1 (MQ=255)
                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTc                                                                                          >  2:10073674/1‑52 (MQ=255)
                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  1:1107702/1‑137 (MQ=255)
                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  2:6396655/1‑137 (MQ=255)
                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  1:10515813/1‑137 (MQ=255)
                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  1:15898126/1‑137 (MQ=255)
                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  1:8237230/1‑137 (MQ=255)
                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  1:3640113/1‑137 (MQ=255)
                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  2:498875/1‑137 (MQ=255)
                                                                                                                                   |                                                                                                                                    
ACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712929‑1713192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑