Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 353,067 A→G intergenic (+34/+84) hisF CDS (Imidazole glycerol phosphate synthase subunit HisF) → / ← cbcV CDS (choline _ betaine _ carnitine ABC transporter ‑ ATP binding subunit) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1353,0670AG100.0% 1366.4 / NA 359intergenic (+34/+84)hisF CDS (Imidazole glycerol phosphate synthase subunit HisF)/cbcV CDS (choline _ betaine _ carnitine ABC transporter ‑ ATP binding subunit)–/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (170/189);  total (170/189)

Only 100 of 361 total aligned reads displayed.

TGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCG  >  AE015451_phaM1/352934‑353199
                                                                                                                                     |                                                                                                                                     
tGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGc                                                                                                                                    <  1:4908288/137‑1 (MQ=255)
tGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGc                                                                                                                                    <  2:1542200/137‑1 (MQ=255)
 gCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCa                                                                                                                                   >  1:9247438/1‑137 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGTACg                                                                                                                                 >  1:16878873/1‑137 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                 <  2:10970162/137‑1 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                 <  1:5550806/137‑1 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTCCGCGGGCACg                                                                                                                                 >  2:6824324/1‑137 (MQ=255)
   cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATCGACCGGGGCTGCTTTGCAGCCCTCCGCGGGCACg                                                                                                                                 >  1:6524947/1‑137 (MQ=255)
    aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                <  1:12608253/137‑1 (MQ=255)
        cgGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGc                                                                                                                            <  1:4312997/137‑1 (MQ=255)
         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGAACGCCCACt                                                                                                                           >  1:1505414/1‑137 (MQ=255)
         ggTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGAACACCCACt                                                                                                                           >  1:15803121/1‑137 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑c                                                                                                                         <  1:9730306/137‑1 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑c                                                                                                                         <  2:15071745/137‑1 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑c                                                                                                                         <  1:6148757/137‑1 (MQ=255)
          gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑c                                                                                                                         <  1:8674290/137‑1 (MQ=255)
              tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACGTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGTCCTTCGCGGGCCCGCCCGCT‑CCcaa                                                                                                                     >  2:14668094/1‑136 (MQ=255)
              tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGTACGCCCGCT‑CCcac                                                                                                                     >  2:3722637/1‑137 (MQ=255)
              tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGGACGCCCGCT‑CCcac                                                                                                                     >  1:16579676/1‑137 (MQ=255)
              tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACACCCGCT‑ccccc                                                                                                                     >  1:1413691/1‑135 (MQ=255)
               tgcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCcaca                                                                                                                    <  2:8583699/136‑1 (MQ=255)
               ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCcaca                                                                                                                    <  2:1867172/137‑1 (MQ=255)
               ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCcaca                                                                                                                    <  2:1415713/137‑1 (MQ=255)
               ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCcaca                                                                                                                    <  2:13491914/137‑1 (MQ=255)
                   gcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCC‑CCCACAGGtt                                                                                                                >  1:13524596/1‑137 (MQ=255)
                    ccAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGttt                                                                                                               <  2:115315/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                            <  2:12169726/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                            <  1:7056239/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                            <  2:7499943/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                            <  1:16059506/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                            <  2:14405931/137‑1 (MQ=255)
                       gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                            <  1:3414112/137‑1 (MQ=255)
                       acATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                            <  2:16163649/136‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  2:7333239/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  2:8952715/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  1:16455999/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  1:11468102/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  1:12055964/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  2:16497/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  1:3043451/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  1:13147117/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  1:14325848/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  1:4997128/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACT‑CCCACAGGTTTGCTga                                                                                                          <  2:8334154/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTGCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  2:4523351/137‑1 (MQ=255)
                         aTCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACACGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCATAGGTTTGCTga                                                                                                          <  2:8952751/137‑1 (MQ=255)
                           cTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTgaga                                                                                                        >  2:8015065/1‑137 (MQ=255)
                              ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGAtct                                                                                                     <  1:16239306/137‑1 (MQ=255)
                               cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCATCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGAtctc                                                                                                    <  1:7821834/137‑1 (MQ=255)
                                   ttGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCtgtg                                                                                                <  1:1859076/137‑1 (MQ=255)
                                   ttGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCtgtg                                                                                                <  1:10970504/137‑1 (MQ=255)
                                     ggCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCTCT‑CCCACAGGTTTGCTGAGATCTCTTTggg                                                                                              >  1:8141288/1‑137 (MQ=255)
                                       cGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCCCAAGGTTGCTGAGGTCTCCTTGGGAg                                                                                            >  1:379450/1‑137 (MQ=38)
                                                   gCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGAACGACCGCT‑CCCACAAGGGTGGTGGGATGTCTGTGGGGGAGGGAGTGtcca                                                                                >  1:10578349/1‑136 (MQ=25)
                                                    ctgggggCAAAGGCTGACATGTTTTCTCGCGGGATCTTCGTTTGCTGATCCATTGACCGGGGTTGTTTTGCAGCCCTTCGTGGGCACTCCCCCT‑CCCACAGTTTTGCTGAAACCTCTGTGGGAGCGGACTTGCCcgc                                                                               <  2:3224635/132‑1 (MQ=255)
                                                     cGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAAGTTTTCTGGGATCTCTGTGGGAGCAGGCGTGCCcacg                                                                              >  1:6875075/1‑137 (MQ=38)
                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGTTGAGATCTCTGTGGGAGCGGGCGTGCCCACGaa                                                                            >  1:13841210/1‑137 (MQ=255)
                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGaa                                                                            >  2:12004236/1‑137 (MQ=255)
                                                       gAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCAAAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGaa                                                                            >  2:3979219/1‑137 (MQ=255)
                                                        aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCTCT‑CCCAAAAGTTTGTTGAGAGCTCTGTGGGAGAGGGCATGCCCGCGaaa                                                                           >  1:7290960/1‑136 (MQ=38)
                                                        aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                           >  2:12456622/1‑137 (MQ=255)
                                                        aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                           >  2:2526672/1‑137 (MQ=255)
                                                        aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCAAAGGGTTTTTGAGGTCTCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                           >  2:5423613/1‑137 (MQ=38)
                                                        aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCC‑CCCACAGGTTTTATGAGATCTCTGTTGGAGCGGGCATGCCCGCAAAg                                                                           >  1:9036348/1‑137 (MQ=38)
                                                        aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGAACTCCCGGC‑CCCCAAGGGTTGCCGAAGGCTCTATGGGAGTGGGTGTGTCCGCGaaa                                                                           >  1:14974173/1‑136 (MQ=255)
                                                               ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGGACGGCCACC‑CCCCCAAAGTTGTTGGGGGTTCTTTGGGGGTGGGGGTGTCCGCTAAGGGCcgct                                                                    >  1:406233/1‑136 (MQ=255)
                                                               ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCcacg                                                                    >  2:4034383/1‑137 (MQ=255)
                                                               ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACC‑CCCACAAGGTTGCTGAAGTCTCTGGGGGGATAGGTGTTTCCGCGAAGGGCcgcg                                                                    >  1:2654688/1‑137 (MQ=25)
                                                               ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGAACATCCACT‑CCCCCAGGGTTTTTGAGGTTTCTGGGGGAGTGGGGGTGCCCCAGAAGGGGcgcg                                                                    >  1:9985608/1‑137 (MQ=255)
                                                                 ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCACA‑GTTTGTTGAGGTCTCCATGGGAGCAGGCCTGCCCACGGCAGGCCGCGaa                                                                  >  2:13173379/1‑137 (MQ=25)
                                                                 ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑c                                                                                                                         >  1:5137845/1‑82 (MQ=255)
                                                                 ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          >  2:15138685/1‑97 (MQ=255)
                                                                 ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGACCACT‑CCCCCAAGGTTGCTGAGAGCCCCTTTGGAGAGGGAGTGCCCGCTAAAGGCCGCaaa                                                                  >  1:10893327/1‑137 (MQ=25)
                                                                       tGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CTCACAGGTTTGTTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGcc                                                            >  2:6279048/1‑137 (MQ=255)
                                                                        ggCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCACGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGccc                                                           <  2:5196956/137‑1 (MQ=255)
                                                                         tcTTCGCGCGGGCTCGTCGTTCGCTGCTCCGTTGACCGGGGCTTCTTTGCAGCCCTCCGTGGGCACGCCCTCT‑CCCACAGGTTTGCGGAGACCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGcccc                                                          <  2:3488418/136‑1 (MQ=37)
                                                                         tcTTCGCGCGGGATCGTTGTTCTCTGATCCATTGACTTGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGACCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGcccc                                                          <  2:15874715/136‑1 (MQ=38)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCCCAGGTTTGTTGGGATCTCTGTGGGAGAGGGCGTGCCCACGAAGGGCCACGAAACGGCCCCaact                                                      >  2:5115877/1‑135 (MQ=37)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCAAAGGTTTGCTGAGATCTCTGTGGGAGCTGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAAtt                                                      >  2:10530225/1‑137 (MQ=255)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGACCGCT‑CCCCCAAGGTTGATGAGAGCTCTGTGGGAGAGGGGGTGCCCACAAAGGGTCACGAAAAGGACCCaaat                                                      >  1:10113152/1‑135 (MQ=21)
                                                                             cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGAGCACACCCACT‑CCCACAAGTTTGCCGAGACCTCTGTGGGCGCGGGCGTGCCCGCGCAAGGCCGCGACGCAGCCCCAAtt                                                      >  1:7334405/1‑137 (MQ=37)
                                                                              gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTg                                                     >  2:9680077/1‑137 (MQ=255)
                                                                                gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCCCAGGTTTGCTGAGGTCTCTGTGGGAGTGGGCGTGCCCGCGAAGGGCCACGAAGCGGTCCCAATTgtt                                                   >  1:15284630/1‑135 (MQ=37)
                                                                                gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCACT‑CCCCCAAGTTTTTTGAGATCTCTGTGGGAGCGGGCGTGCCCCCGAAGGGCCACAAAGCGGCCCCAACTGCt                                                   >  1:7739654/1‑137 (MQ=25)
                                                                                      tcgtcgTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGTTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAACGGCCCCAACTGCTTCAAcc                                             >  2:1632372/1‑137 (MQ=255)
                                                                                              cGCTGATCCATTGACCGAGACTGCTTTGCAGCCCTTCGCGGACACACCCGCT‑CCCACAGGTTTGCTGAGATCCCTGGGAAAACAGGCGGGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCt                                     <  2:13673017/137‑1 (MQ=37)
                                                                                                    tCCGTTGACTGGGGTTGTTTGGAATCCCTTCGTGGGCACGCCCACT‑CCCACGGTTTTGTTGAGCTCTCTGTGGGAGCGGGTGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGa                               <  2:10296873/137‑1 (MQ=25)
                                                                                                       gttGACTGGGTTTGTTTTGCATCCTTTGGTGGGCACTCCCGCT‑CCAACGGTTTGGCGGAGATCTTTGGGGGAGTGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagca                            <  1:12238667/136‑1 (MQ=19)
                                                                                                       aTTGACTGGGGCTGTTTTGCAGCCCTTCGCGGGCACGCCAGCT‑CCAACGGGTTTGCGGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagca                            <  2:12896868/137‑1 (MQ=38)
                                                                                                        ttGACTGGGGTTGTTTGGCACCCTTTCTCGGGAACGCCCGCC‑CCCCCGGTTTTTCTGAAATCTCTGGGGGAGTGGGTGTGCCCGTGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagcag                           <  2:13281655/137‑1 (MQ=255)
                                                                                                         tGACCGGGGCTGTTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGTTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagcagc                          <  2:6478515/137‑1 (MQ=255)
                                                                                                            ccGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTTTGGGGGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAATAGcgcg                       >  2:11362451/1‑137 (MQ=38)
                                                                                                                ggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACCGGGTTGCTGAGATCTCTGTGGGAAAGGGCATGCCCGCAAAGGGCCACGAAGCAGCCCCAACTGCTTCCACCGTGGTTTTTTCCGAGCAACACGTGGt                   >  2:13692327/1‑137 (MQ=25)
                                                                                                                 gctgctTTGTACCCCTTCTCGGCCACGCCCTCT‑CCCACAGGTTTGCTGAGACCTCTTTGGGAGCGTTCTTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTa                  <  2:2169222/137‑1 (MQ=37)
                                                                                                                  ctgctTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGGTGGGATCTCTGTGGGAGCGGGCGTGCCCACGAAGGGCCGCGAAGTGGACCCAATTGCTTCCACCGTGGTTTTTTCCGAGCAGCGCGTGGTaa                 >  2:5894531/1‑137 (MQ=37)
                                                                                                                  ctgctTTGCAGCCCTTCGCGGGCACGCCCACT‑CCCACAGGTTTGCTGAGATCTCCGTTGGAGCAGGCGTGCCCACGAAAGGCCGCGAAACTGCCCCAACTGCTTCAAACAGGGTTCCTGCCAAGCAGCGCGTGGTaa                 >  1:11662514/1‑137 (MQ=25)
                                                                                                                     ctTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGGGGTaaaac              >  2:3068985/1‑135 (MQ=255)
                                                                                                                     ctTTGCAGCCCTTCGCGGGCACGCCCGAT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAACGCGTGGTAAAGc              >  2:7096688/1‑137 (MQ=255)
                                                                                                                        tGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTga                                                                                                          <  2:14047763/42‑1 (MQ=255)
                                                                                                                            ccccTTCGCGGGCACCCCCGCT‑CCCACAGGTTTGTTGAGCTCCCTGTGGGAGTGGGTGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTAt       <  2:4249569/136‑1 (MQ=38)
                                                                                                                                     |                                                                                                                                     
TGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCG  >  AE015451_phaM1/352934‑353199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑