| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,499,508 | +C | intergenic (+85/‑79) | trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,499,506 | 1 | . | C | 91.0% | 783.9 / 84.8 | 258 | intergenic (+83/‑81) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base . (17/6); new base C (167/67); total (185/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 270 total aligned reads displayed.
GATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTG‑TT‑T‑CGGG‑CA‑C‑G‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTC > AE015451_phaM1/1499373‑1499637 | gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGTGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TT‑CGCGGG‑CA‑c‑g‑cc > 2:8526317/1‑137 (MQ=25)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACC‑GGCTTTTCCGGTGTTT‑T‑CGGG‑CA‑c‑g‑cc > 2:2232358/1‑137 (MQ=37)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCACGGTGCCCGGCCCC‑CGCTTTTCCGGTG‑TTCG‑CGGG‑CA‑c‑t‑cc > 2:9334172/1‑137 (MQ=37)gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAACGGCGCCAAGGTTCACTGCCCC‑AGATTTGCCGGTG‑TTCT‑CAGG‑CA‑a‑g‑cc > 1:16820284/1‑137 (MQ=255) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑c‑g‑cccg > 2:16520937/1‑135 (MQ=25) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGCGCCACGGTGCATGGCAAC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCcac > 2:2335353/1‑137 (MQ=21) gagCCTGAACGAATCCTTGGAACCACAGCGGGA‑CTGAGGCTGCCAAATGGTGGGAGTGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGACACC‑GGCTTTGCCGGTG‑TT‑CGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACt < 2:2156795/137‑1 (MQ=18) cTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAAGCGCCGCGGTGTATGGCACC‑GGATTTTCCGGTG‑TTCT‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGAATGAtt > 1:6997782/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTTCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGc > 2:9123087/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGCGCCACGGTGCACGGCACC‑AGCTTTTCCGGTT‑TTCG‑CGGG‑CA‑C‑GCCC‑ACCCCCACAAACTGATTGc > 1:8043030/1‑137 (MQ=255) cGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑‑T‑T‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTa > 1:16329588/1‑137 (MQ=25) cGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCACCGCGGTGCATAGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑A‑A‑CCAGTTCCCAAAGAATGATTGCCAt > 2:14535565/1‑137 (MQ=18) gAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAAACGCCCCGGTGCATGGCACC‑AACTTTTCCGGTGTTT‑T‑TGGG‑CA‑C‑GCCC‑ACCCCCCCAGAATGATTGTCAtt > 1:11041004/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCACGGTGCCTGGCACC‑AGCTTTGCCGGTG‑TTCT‑CGGG‑CA‑CAC‑CC‑GCTTCCACAGAATGATTGACAtt > 1:13053385/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAAGAGCCACGGGGCATGGCACC‑GGCTTTTCCAGTG‑TTCG‑CGGG‑AA‑CAC‑CC‑GCTCCCCCAAACTTATTGCCact > 2:15674160/1‑135 (MQ=12) gAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGATTGCCCTCCAAACCAGCGCCACGGTGTCTGGCACC‑GGCTCTGTCCGTG‑TTCT‑CGGGAAA‑A‑C‑CC‑ACTCCCCCAAACCGATTGCCact > 1:4714848/1‑135 (MQ=255) gAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCAAAAAGGTGGGAGCGGGCGCGCCCACGAAACAGGCACCGCGGTGCATGGCACC‑GGATTTGCCGGTG‑TTCT‑CGGG‑CA‑C‑GCCC‑GCTCCCACGGACGGATTACCAtt > 2:13146225/1‑137 (MQ=18) aGCCTTGTCACCACAGCAGGA‑CTGGGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACGGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTaa < 2:16948129/137‑1 (MQ=18) ccTTGTCACCACAACAGAA‑CGGAGGCTTACAAACGGTGGAAGCGGGCGTGCCCGCGAAACAGGCACCACGGTGCATGGCACC‑GACTTTTCCGTTG‑TTCC‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGc < 2:12043863/137‑1 (MQ=12) ttGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGGGCCGAGGTGTATTGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGAATGAGTGACATTCAGCgg > 1:4206868/1‑137 (MQ=17) ttGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAACAAGCACAGCAGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCAAAGAATGATTGCCCTTCAGTgg > 2:13255893/1‑137 (MQ=12) gCACCACAGCAGGA‑CTGAGTTTGCCAAACGTTGGGAGCGGGCGTGCCCGCGAAAAGGGCGCCGTGGTGCATGGCACC‑GGTTTTGCCGTTT‑TTCG‑CGGG‑CA‑C‑TCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:11945443/137‑1 (MQ=18) ccaccacAG‑AGGACCTGCGTCTGCAAAACGGTGGGAGCGGGTGTGCCCGTAAAAAAGGTCCCATGGTCCCTGACCCT‑GGCTTTGCCGGTG‑TT‑CGTGGG‑CA‑C‑ACCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:5791/136‑1 (MQ=255) caccacAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GATCCCACAGACTGATTGCCATTAAGCGGGACt > 2:10402044/1‑137 (MQ=18) caccacAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCACGAAAAAAGAACCACGGTGTCTGGCACC‑CGATTTTCCGGTGTTT‑T‑CGGG‑GA‑CAC‑CC‑CCCCCCCCAAAATGATTGTCACTCAGCGGGGCt > 1:14649028/1‑137 (MQ=255) gcagGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACC‑GGCCTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTTCCACAGAATGATTGCCATTCAGCGGGCCTATCGCTa > 2:15912533/1‑137 (MQ=18) cagGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGGAACAGGCGCCGCGGTGCATGGCACC‑AGATTTGCCAGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCCCAGACTGATTGCCATTAAGCGGGTCTATCGCtcc > 2:6118829/1‑135 (MQ=18) cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑A‑GCCC‑GCTCCCCCAGAATGATTGCCATTAAACCGGCCCCTCGCTGCAGTgg > 2:13436845/1‑136 (MQ=14) tGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAAGCGCCGCGGTGCACGGAACC‑GGCTTTGTCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCCCCCAAAGAATGACTGCCATTCAGCGGGTCTATCGCTCCAATGAc > 1:12049481/1‑137 (MQ=12) gggCTGCCAAATGGGGGGAGTGGGTTTGCCTGGGAAAAGGGTGCCGCGGTGCGGGGCACT‑GGTTTTGCCGGTG‑TT‑TGTGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg < 1:10344492/136‑1 (MQ=255) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑ACTCCCACAGAACGGGTGCCATTAAGCGGGCCTCTCATTACAATGACgg > 2:4534108/1‑137 (MQ=14) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑TT‑CGCGGG‑CA‑A‑GCCC‑GCTCCCACAGAATGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc > 2:1747795/1‑137 (MQ=18) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑AGCTTTGCCGGTG‑TTCG‑CGGG‑CC‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc > 2:8418754/1‑137 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCCCC‑GGATTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCCCAAACTGATTGCCATTCAGCGGGCCTATCGCTACATTGACGGCCCTtttt > 2:11132556/1‑137 (MQ=18) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGAGGTGCAGGGCACC‑GGCTTTGCCGGTG‑TTCG‑AGGG‑CA‑A‑GCCC‑GCCCCCACAGAATGATTGCCATTAAGCGGGACTCTCGCTACAATGACGGCCCGtttt > 2:16489255/1‑137 (MQ=17) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCATGGCACC‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAAACTGATTGCCATTAAGCGGGACTATCGCTACAGTGACGGCCCGtttt > 2:6236668/1‑137 (MQ=17) aCGTTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCTGGGTATGGCACC‑GGTTTTGCCGGTG‑TT‑CGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 2:2605183/137‑1 (MQ=21) ggggggAGTGGGTGTGCCCGAGAAAAGGGCGCTGGGGGGCGT‑GCCACTGGTTTTGCTGGTG‑TTCG‑GGGG‑CA‑C‑CCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 1:6448523/134‑1 (MQ=255) ggTGGGAGAGGGCGTGCCTGCGAAACGGGCGCTGCGGTGAATGGCACC‑GGCTTTGCGGGTG‑TT‑CGCGGG‑AACC‑C‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 2:631284/137‑1 (MQ=255) ggAGCGGGCGTGCCCGCGAAACAGGCGCCGTGGTGCATGGCACC‑GTCTTTGCCGGTG‑TT‑AGCGGA‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 2:6774309/137‑1 (MQ=21) gCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACC‑AGCTTTTCCAGTG‑TTCG‑CGGG‑CA‑CAC‑CC‑ACTCCCCAAAACTGACTGCCATTAAGCGGGCCTCTCGCTCCAATGACGGCCCGTTTTTTTTTTCCTCg > 2:5656582/1‑137 (MQ=12) cGTGCCCGCGAAACAGGCGCCACGGTGCATTGCACC‑GGCTTTTCCCATG‑TTCT‑CGGG‑CA‑CAC‑CC‑ACTCCCACAGACTGATTGTCATTCAGCGGGCCTCCTGCTACAATGACAGCCCGTTTTTTGTTTCCCCACTAAc > 2:12646643/1‑137 (MQ=255) gTGCCCGAGAAACGGGCGCCGTGGTGCAGGGCACT‑GGTTTTGCCGGTG‑TT‑TGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACg < 1:12740911/137‑1 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GTCTTTGCAGGTT‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 2:17178478/137‑1 (MQ=25) cgcgAAACGTGCGCCGCGGGGCATGGCACT‑GGTTTTGCCGGTG‑TT‑CGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:1851752/137‑1 (MQ=25) cgcgAAACGGGCGCCGTGGTGCGTGGCACC‑GTTTTTGCTGGTG‑TT‑CGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:484541/137‑1 (MQ=25) cgcgAAACGGGCGCCGCGGTGAGTGGCACC‑GGCTTTGCCGGTG‑TTCG‑GGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:2627045/137‑1 (MQ=25) cgcgAAACAGGCGCCGCGTTGCATGGCACC‑GGTTTTGCCGGTG‑TT‑CGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:2016868/137‑1 (MQ=37) cgcgAAACAGGCGCCGCGGTGAATGGCACT‑GGTTTTGCCGGTG‑TTCG‑CGGA‑CA‑C‑CCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:3080486/137‑1 (MQ=255) cgcgAAAAAGTCGCTGTGGTGAGTGGCACT‑GGTTTTGCGGGTG‑TT‑TGCGGA‑CA‑C‑CCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:558772/137‑1 (MQ=255) cacgAAAAAGGCGCCGTGGGGCGTGGCACT‑GTCTTTTCCGGTG‑TT‑TGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:6906198/135‑1 (MQ=25) cgccgtGGTGCATGGCACC‑GGTTTTGTTGGTG‑TTCG‑CGGA‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:9522607/137‑1 (MQ=255) cgccggGGGGCATGGCACT‑GGTTTTGCTGGTG‑TT‑CGCGGG‑CA‑C‑CCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:14915996/137‑1 (MQ=255) caccgcGGCGCATGGCACC‑GGTTTTGCTGGTG‑TT‑TGGGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:10198965/135‑1 (MQ=37) cATGGCACC‑GGCTTTGCCGTTG‑TTCT‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 1:17280017/137‑1 (MQ=38) acc‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 2:8961495/3‑137 (MQ=38) acc‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 1:2375555/3‑137 (MQ=38) c‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:16772680/1‑137 (MQ=38) c‑GGCTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCAACAGACTGATTGCCCTTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCATCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGTAGAt > 2:2758059/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACCGACTGCTTGCCCTTAAGCGGGCCTCTCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAATCCCTTAGAACGCTCTCAAGCAGATCTg > 2:4245061/1‑137 (MQ=37) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTATAACGCTATCAAGCAGATCTg > 2:4080456/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:3008480/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:5083728/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:6969436/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:7205293/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:9808887/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:2841450/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:10093460/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:10669867/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:3519442/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:4445103/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:15601554/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:13705614/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:14392878/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:6843968/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:9759177/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:15093673/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:12197098/1‑137 (MQ=39) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:17556756/1‑137 (MQ=38) cTTTGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGACCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCACTTAGAACGCTATCAAGCAGATCTg > 2:8762547/1‑137 (MQ=38) cTTTGCCGGTG‑TT‑CGTGGG‑CA‑C‑G‑CCAGCTCCCACAGACTGATTGCCATTAAGCGGGCATATCGCTACAGTGACGGCCCGTTTTTTATTGCCTCGCTAACGAATCATGACTCACTTAGAACGCTATCGAGCAGATCTg > 2:10855645/1‑137 (MQ=255) cTTTGCCGGTG‑TT‑CGCGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:14100283/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 1:9042898/124‑1 (MQ=38) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:7768816/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:7814361/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:13157796/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:5559036/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:1256290/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:12859773/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:14633632/1‑137 (MQ=39) ttGCCGGTG‑TTCG‑CGGG‑CA‑C‑GCCC‑GCTCCCACAAACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGGCTCCCTTAGAACGCTATAAAGCAGGTCTGaa > 2:7114176/1‑137 (MQ=38) tcgcGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 2:15102024/4‑93 (MQ=255) tcgcGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc > 2:3335374/4‑137 (MQ=255) tcgcGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGACCTGAAACGTCCCGAc > 2:1789650/4‑137 (MQ=255) cgcGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 2:3444218/3‑137 (MQ=255) cgcGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 2:1613518/3‑137 (MQ=255) cgcGGG‑CA‑C‑GCCC‑GCTCCCACAGACTGACTGCCATTAAGCGGGCCTATCGCTACCGTGACGGCCCGTTTTTTGTTGCCTCGCTACCGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCAGAct > 2:2931630/3‑137 (MQ=255) ggg‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGa < 1:10015718/85‑1 (MQ=255) ggg‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa > 2:3752797/1‑102 (MQ=255) g‑CA‑C‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACttcttc > 2:8153757/1‑137 (MQ=255) | GATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGA‑CTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTG‑TT‑T‑CGGG‑CA‑C‑G‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTC > AE015451_phaM1/1499373‑1499637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |