Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,681,415 +CGGG intergenic (‑133/+71) PP_4986 CDS (Channel protein_ hemolysin III family) ← / ← PP_4987 CDS (putative Chemotaxis protein) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,681,4151.C100.0% 312.2 / NA 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (71/15);  total (71/15)
*AE015451_phaM15,681,4152.G98.8% 312.9 / NA 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (69/15);  minor base A (1/0);  total (71/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM15,681,4153.G97.7% 309.4 / ‑4.4 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (69/15);  minor base A (2/0);  total (71/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM15,681,4154.G100.0% 318.6 / NA 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (71/15);  total (71/15)

Only 100 of 102 total aligned reads displayed.

ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC  >  AE015451_phaM1/5681279‑5681530
                                                                                                                                           ||||                                                                                                                   
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  2:10081155/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  2:5434764/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:6340213/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:8928243/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  2:10081126/137‑1 (MQ=255)
aCATACAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:2480324/137‑1 (MQ=255)
    gCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccggg                                                                                                                     <  1:6919/137‑5 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGGTGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:6229929/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  1:4215292/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:7363467/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:6229891/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:7571098/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:1852175/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:1851799/137‑6 (MQ=255)
          acaGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:16856419/127‑1 (MQ=255)
          acaGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         >  2:16856419/1‑127 (MQ=255)
                   cttaGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGTGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGccc                                                                                                      <  2:8722025/134‑1 (MQ=37)
                    tGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCt                                                                                                     >  2:5252850/1‑137 (MQ=37)
                     gagttGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:13411725/133‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTTTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:2714552/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:14556221/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:5974397/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:14964073/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:2409052/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:238946/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:12296190/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:6781079/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:723655/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:12095756/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:11822561/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTAATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:5128752/137‑1 (MQ=37)
                                ggTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGacac                                                                                           >  2:2460001/1‑137 (MQ=37)
                                ggTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGAAT‑‑TTTGTCGTGGAAGGGGGGCGAAGCGGCCCCAGGTTTTCATTTTTACCACATGAAACGCCAGGGCTGCTGCGCGGCCCTCCCGCGACACaa                                                                                         >  2:11723773/1‑137 (MQ=255)
                                        ttgtGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGTCT‑‑TGTTTCGCTAAAGGGGAGCGGAGCAGCCCCCGGCTTTTCCTGGCACTGCACGAACCGCCTAGGCTGCTGTGTAGACCCCTCGCGACACAAGGCTGccc                                                                                 >  2:16739792/1‑135 (MQ=255)
                                                      gCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTNCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:17016609/104‑1 (MQ=21)
                                                      gCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    >  2:17016609/1‑104 (MQ=25)
                                                                                           agggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGCCAgg                                >  2:11180528/3‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCCGGCTTTCATTGCCCCTGCATGAACCGCCGGGGCTGCTTCGCAGCCATATCGCGACACAAGGCCGCCCCCACTCCAGACCACCAGGCCCTGCGCATCCCATCCTCACCAAGGTCAgg                                >  1:6216686/1‑137 (MQ=25)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTCTCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTACCCAAGGTCAgg                                >  2:16643336/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCCGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:2030152/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCGAGGTCggg                                >  2:13748265/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCACGGTCAgg                                >  1:10683428/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:15575164/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:16542238/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:13765638/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:16708577/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:4731243/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:5762194/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:6514954/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:7153167/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:9485854/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:13964664/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:8872527/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:8195999/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:16721276/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:16877790/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:16877791/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:17156932/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:1969386/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:11258741/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:3196605/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:3196650/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCACCTCACATCCTAACCAAGGTCAgg                                >  1:11033762/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAAACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:7580492/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTANCGCGACACAAGGCCGCTCCCCCTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:15195180/1‑137 (MQ=38)
                                                                                             ggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGtt                              >  1:244510/1‑137 (MQ=38)
                                                                                             ggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGtt                              >  1:12886663/1‑137 (MQ=38)
                                                                                                  gcgAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGa                         >  2:4205431/1‑137 (MQ=38)
                                                                                                  gcgAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGa                         >  1:14039546/1‑137 (MQ=38)
                                                                                                    gAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc                       >  1:6437302/1‑137 (MQ=38)
                                                                                                    gAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc                       >  2:6520685/1‑137 (MQ=38)
                                                                                                    gAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc                       >  1:2292117/1‑137 (MQ=38)
                                                                                                        cGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCcgcg                   >  1:16115150/1‑137 (MQ=38)
                                                                                                             ccAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACg              >  1:9077445/1‑137 (MQ=38)
                                                                                                             ccAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACg              >  1:298202/1‑137 (MQ=38)
                                                                                                             ccAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACg              >  1:298236/1‑137 (MQ=38)
                                                                                                                   tttCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAg        >  1:3295579/1‑137 (MQ=38)
                                                                                                                       aTTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGNCCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCtt    >  1:15219483/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACCCCAGGCCGCTCCCCCTCCAAACCAACCGGCCCTGCGCACCACACCCTACCCAAGGTCAGGCTGCAAATCCGCGTCACGCACCAGCCtgg   >  2:9482473/1‑135 (MQ=19)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:11197343/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:11731685/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:12049258/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1349395/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:11197342/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:5392493/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1349396/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1349436/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:5623363/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:2881354/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:2881353/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:14453516/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1720331/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:15372936/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACNCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:16260305/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCACTATCGCGACACAAGGCCACTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCCCGCACCAGCCttt   >  2:6868227/1‑137 (MQ=255)
                                                                                                                        ttGCCACTGCATGAACCGCCGAGGCTACTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCAATCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCCAGGTCCGGTTGCAGCTCCACGTCACACACCAGCCttt   >  2:5672237/1‑137 (MQ=37)
                                                                                                                        ttGCCACTGCATAAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCCGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:14453481/1‑137 (MQ=37)
                                                                                                                         tGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTc  >  1:6398990/1‑137 (MQ=38)
                                                                                                                         tGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTc  >  1:6398955/1‑137 (MQ=38)
                                                                                                                         tGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTc  >  1:5069640/1‑137 (MQ=38)
                                                                                                                                           ||||                                                                                                                   
ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC  >  AE015451_phaM1/5681279‑5681530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑