BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,344,308	0	G	A	37.1%	77.5 / 25.3	110	intergenic (+39/‑117)	PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base G (47/14); new base A (36/0); total (95/15)
Fisher's exact test for biased strand distribution p-value = 1.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 148 total aligned reads displayed.

CGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344172‑4344443

  ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCGATCGAGCAccgcc                                                                                                                                       >  2:15509591/1‑137 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCCGTCCGAGCGAGCAcccccc                                                                                                                                      >  1:8402831/1‑137 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:7224833/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:15376581/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:641425/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:10824030/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  2:6920178/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:7426596/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:15160097/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:12797168/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:12750192/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:11169003/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATAGAGCAccgccc                                                                                                                                      <  1:8787066/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCAGTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgccc                                                                                                                                      <  1:12797166/137‑1 (MQ=255)

    cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAGATCGAGAaccgaccg                                                                                                                                     >  1:12829810/1‑137 (MQ=255)

    cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  2:11821830/137‑1 (MQ=255)

    cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  1:10330906/137‑1 (MQ=255)

    cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  1:10330864/137‑1 (MQ=255)

    cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCAccgcccg                                                                                                                                     <  1:2441953/137‑1 (MQ=255)

     gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATCAACGCTCTTGTACGTTGCAGT‑GCCTGCCAGATCAAACCccgccccc                                                                                                                                    >  2:4314600/1‑135 (MQ=255)

      aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGTCCGATCGAGCACCACCcacg                                                                                                                                   >  1:17068381/1‑137 (MQ=255)

      aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCCccacccccc                                                                                                                                   >  1:10264373/1‑134 (MQ=255)

      aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcg                                                                                                                                   >  2:7311680/1‑137 (MQ=255)

       cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTCCCGACCAAGCAccccccaagc                                                                                                                                  >  1:12188011/1‑133 (MQ=255)

       cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTTCCCGACCGAGCAccacccgcac                                                                                                                                  >  1:15013809/1‑135 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:15791545/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:9913840/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  1:4803068/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  2:817962/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGACAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 <  2:12033257/137‑1 (MQ=255)

          cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                               <  2:8634390/137‑1 (MQ=255)

            tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCCGCCAGATCGAGCACCGACCACACGGcac                                                                                                                             >  1:7636194/1‑137 (MQ=255)

            tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCAGcac                                                                                                                             >  1:10201634/1‑137 (MQ=255)

            tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAAACCGAGCACCGCCCGGGCGGaac                                                                                                                             >  2:12034210/1‑137 (MQ=255)

              aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                           <  1:1126763/137‑1 (MQ=255)

              aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                           <  2:6549041/137‑1 (MQ=255)

               aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGATGCCCAATCGAGCAACACCCGCACGGGacaca                                                                                                                          >  1:3329406/1‑136 (MQ=255)

               aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTGCCAGATCAAGCACCGCCCGCGCGGCAAACg                                                                                                                          >  1:9829399/1‑137 (MQ=255)

                ggTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAGATCTAACACCACCCACGCGGCACACGa                                                                                                                         >  2:15561102/1‑137 (MQ=255)

                    cGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCTAGCACCGCCCACGCGGAACACGAGCTg                                                                                                                     >  2:3751238/1‑137 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGCCCAGTCTAGCACCGCCCGTGCGTCACCCAGTCTGGCa                                                                                                                  >  2:12786677/1‑137 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCCGATCGAGCACCGCCCACGAGGCACACGACCTGGCa                                                                                                                  >  2:16513849/1‑137 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAGATCGAGCACCACCCACACGGCCCACAATCTGGCa                                                                                                                  >  1:6665257/1‑137 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATCAACACTCTTGTACGCTTCAGC‑GCCTCCCAAATCCAGCAACGCCCGCACGTTACACACTCTgggc                                                                                                                  >  2:11092606/1‑135 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCACTAACGATCTTGTACGTTGCAGC‑GCCTGACAAGGCGAGCACCACCCACGAGGAACACGATCTGGcg                                                                                                                  >  2:11092607/1‑136 (MQ=255)

                         ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTTTCCAATCGAAAACCGCCCACGCGGAACCCAACCTGGCAgg                                                                                                                >  1:16539675/1‑137 (MQ=255)

                            aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCAAACAAAAACCACACACACGGCACACGACCTGGCAGgcgc                                                                                                             >  1:4088964/1‑137 (MQ=255)

                               ggCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCCGATCGAGCACCACCCACGCGGCACACGATCTGGCAGGCACTGc                                                                                                          >  2:4151421/1‑137 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGCCCGATCGAGCACCACCCACGCGGAACACGATCTTGCAAGAGCttccc                                                                                                        >  1:4084018/1‑133 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCCGACCAAACAACCACCACACAGCACACAACCTTGCAGGCGCTGCaa                                                                                                        >  1:14118613/1‑136 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGTCAGATCGAGAACCGCCCACGCGGCACCAAACCTGGCAAGCGCTGAAc                                                                                                        >  1:3346530/1‑137 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGACCGAACACCACCCACGCAGCCCACGACCCGGCAGGCGCTGCAc                                                                                                        >  2:14036945/1‑137 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCTCCCGACCGAGCACCACCCCCGCGGACCACAACCCTGCCGGAGCTGCAc                                                                                                        >  1:10629226/1‑137 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCCGATCGAGCACCGCCCACGCGGCAAACCATCTTGCCGGCACTGCAc                                                                                                        >  2:8011264/1‑137 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGT‑GCCTGCCAGATCGAGCACCGCCCTCGCGGCGCACAATCTTGCAGGCGCTGCAATATTg                                                                                                   >  2:10869127/1‑137 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAAATCGAACACCCCCCACGCGGCAAACGAGCTGGCAAGAGATGCACCATTg                                                                                                   >  1:3364556/1‑137 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGACCAAGCGAGCAACACCCCCGCGGAAAACAAGCTGGAAGGAGGTGCAcccttt                                                                                                   >  1:9258727/1‑132 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGACGCCAGACCGAGCACCACCCACGCGGACCACGACCTGGCAAGAGTTGCACTactt                                                                                                   >  1:11126568/1‑134 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCCAATCCAGCACCGCCCTCTCATCACACAATCTCGCCAGTGCTGCACCAttt                                                                                                   >  1:13596524/1‑136 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCACCCGCGCGGCACACGATCTGGCAGGCGCTGCGCTATTg                                                                                                   >  1:9258690/1‑137 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTGCCCGGCCGAGCACCGCCCACACAGCACAAAACCTGGTAGGCGCTGAAcccttt                                                                                                   >  1:11946168/1‑132 (MQ=255)

                                           cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCCGATCGAGTCCCACCCGCACGGAACACGGCCCGGTAGGCGCTTCACTCTTGGAggg                                                                                              >  2:3142103/1‑136 (MQ=255)

                                             tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGCCAGATTGAGCACCACCCTCGCGGTACACGAGCTTGAAGGCGCTGCCCTCTTGGAGGCGt                                                                                            >  2:1290821/1‑137 (MQ=255)

                                             tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAAACCAAGCACCGCCCGCACGGCAAACAAACTGGCAAGAGCGGGAATAGTGGAGgtgt                                                                                            >  1:17376012/1‑137 (MQ=255)

                                             tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAAATCGAGCACCGCCCACGCAGGACACGAGCTGGCAGGAGCTGCAATATTGGGGGCGt                                                                                            >  2:13729827/1‑137 (MQ=255)

                                             tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GACTTCCCGATCTAGCACCCCCCCCTCGGACCACAAGCTGGCAGGCGCTGCCCTCTTTGAGGGGt                                                                                            >  1:11007787/1‑137 (MQ=255)

                                                   ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCAAGCACCGCCCACGCGGAACACGAGCTGGCCGGGGTTGTACTATTTGAGGAGTACGccc                                                                                      >  2:5382268/1‑137 (MQ=255)

                                                     gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTTCCCGACCGAACACCACCCACGCAGAACAAAAGCTGGCAAGAGATTCACTACTTGAGGAGTACGACCCg                                                                                    >  2:9167589/1‑137 (MQ=255)

                                                        tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCACCCGCGCGGTACAAGACCTTGGAGGGGCTGAAATCTTGGGGGCGTCCACCCCATca                                                                                 >  2:3893145/1‑137 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCAAACCGAGCAACGCCCGCGCGGAAAACAACATAGGAGGCGCCGAACTACTTGAGGAATACACCCCATccc                                                                                >  2:5036389/1‑135 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTTCCAAACCGAGCACCGCCCACGCAGCCCACAACCTGGCAGGCGACGCAATATTGGAGGCGTACGccccagcac                                                                                >  2:2937250/1‑132 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAAACCCACCCGCGCAGGACAAAACCTGGCAGGGGCTGCCCTACTGGGGGCGTCCAcccacgcca                                                                                >  2:255105/1‑131 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGAGCGAGAAACGCCCGCGCGGGCCACGAGCTGGCAGGCGATGAAATATTTGGGGAGTGCGCCCCATcac                                                                                >  2:2644726/1‑137 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCGCCAGATCTAGCACCACCCCCCCGGCACACGATCCTGCAGGCGCTGCACCCTTTGAGGCGTCCACCCCATccc                                                                                >  2:17345166/1‑135 (MQ=255)

                                                           gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCCTCCCAATCTAGCACCGCCCACTTGGCACACGATCTGGAAAGTGCTTTACTCTTTGGGGCGTCCGCCCCGTCACAt                                                                              >  2:14748871/1‑137 (MQ=255)

                                                             cGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GGCTGCCCGACCTAACACCACCCCCACAGCACAAAACCCTGCAAGCGCCGCACCACTTGAGGCGTCCGCCCCATcccactc                                                                            >  2:6189901/1‑134 (MQ=255)

                                                              gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGAGCGAACCCCTCCCGCGTGGCCCCCGATTCGGAAGGCGATGCACCATTGGAGGAATACACCCCGTcaaactcc                                                                           >  2:9065556/1‑131 (MQ=255)

                                                                   cGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGGTCGAGGACCGCCCGCGTGGCACAAGATCTGGAAGGCGCTGTACTGTTGGAGGGGTACGACCAATCACATTatcatcc                                                                      >  2:11581290/1‑136 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTCCGTTGCAGT‑GACTGCCCGATCGAGCACCGCCCGCGCGGGACACGGCCTGGCAGGGGCTTTCCTATTGGAGGTGTATGCCCCGTCAAATTattatcctggca                                                                 >  2:3488450/1‑131 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTTCAGATCTAGCACCGCCCGTGCGGCACACGATTTGGGAGGTGGTGCACTATTGGAGGCGTACGCCCCGTCGCATTATTATCCCGCCa                                                                 >  2:3488413/1‑137 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGTCAGATCGAGCACCGCCCGCGTGGGACCCGATCTGGCAGGCGCTTTCCTACTGGAGGCGTCCACCCCTTCCCATTATCCTCCCGccc                                                                 >  2:14255113/1‑136 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTGCCAAATTGAACACCGCCCGCGCGGTACACAACCTGGCAGGTGCTGTACTATTGGGGGCGTACTCCCCATCACACTATCATCACGCCa                                                                 >  2:15290147/1‑137 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCACCCACGCGGCAAACGAGCCGGCAGGCGCTGCAATCGTGGAGGTGTACTCCCCATCACACTATCACCACGACa                                                                 >  2:13527826/1‑137 (MQ=255)

                                                                         gAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GTCTTCCCAATCGAGCACCGCCCACGCGGCACCCGAGCCTGCAGGCGCTGGAACATTGGGGGCGTACGTCCCGTTGCATTCCTCTCACGCCAc                                                                >  2:17109002/1‑137 (MQ=255)

                                                                                  ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAAAGCGAGCACCGCCCACGCGGCACAAGAGCTGGCAGGGGGCGCACCATTGGAGGCGTACACCCCGTCACACTATCACCCAACCACCGTCGTaac                                                       >  2:12637924/1‑136 (MQ=255)

                                                                                   gCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCTCCCA‑ATCCAACACCACCCCCACAGCACACAATCTGGCAGGCGCTGCACCATTTGGGGGGTACACCCCACCACATTCTCCCCCCACCCCCGTCGTAATc                                                      >  2:7424069/1‑137 (MQ=255)

                                                                                                        tGTACGTTGCAGC‑GCCTGTCAGATCGAGCACCACCCGCGAGGCACACGACTTGGCAAGTAACGCACTATTGGAGGCGTACGCCCCGTCAAACTATCATCCCGCCACTGTTGTCATTTGCCAGCCAACTTTATGCcac                                 >  2:15834026/1‑137 (MQ=255)

                                                                                                          tACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                 >  2:6817973/1‑39 (MQ=255)

                                                                                                                  aGC‑GCCTGCCAAATCGAGCACCGCCCACACGGCACACAACCCTGCAGGCGCTGCACTATTGGAGGCGTACGCCCCATCACAGTATCACTCCGCCACCGTCGTAATCTGTCAGCCAACTCTCTGTCCCAGGTCACCCg                       >  2:11714582/1‑137 (MQ=255)

                                                                                                                  aGC‑GCCTGCCAAACCGCGCACCGCCCGCGCGGAACACGATCTGGAAAGCGCTGCACTATTGGAGGCGTACGCCCCGGCAAAGTATCATCAAGCCACTGTCATAATCTACCAGCCAACTCCATGTCACAGGTCCCCCg                       >  2:8333064/1‑137 (MQ=255)

                                                                                                                             aaaTCGAGCACCACCCCCGCGGCACACGATCTGGCAGGCGCTGTACCCTTTGAGGAGTACGCCCCGTTACACTCTCATCACGCCAATGTCTTCCTCTGCCGGACAACTCTCTGCCACAAGTCACCCGGCATGCTCCg             >  2:3279831/3‑137 (MQ=255)

                                                                                                                               aTCGAGCACCGCCCGCGCGGAAAAAGACCTGGCAGGAGCTGCAACACTGGAGGCGTCCACCCCCTCACACTCTCATCAAGCCACTGTTGTAATCTGCCGGCCAACCCTATGTCACAGGTCACCCAGCATGCttcacc           >  2:14673847/1‑132 (MQ=255)

                                                                                                                               aTCGACCCCCCCCCAAGCGACACACAATTTGCCACCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGcc           <  2:13825952/137‑1 (MQ=255)

                                                                                                                                  gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  2:6149626/1‑137 (MQ=255)

                                                                                                                                    gCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGTACTATTTGAGGCGTACGCCCCGTCACATTATCATCCCGCCACTGTCGTCATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt      >  1:5304724/1‑137 (MQ=255)

                                                                                                                                     cACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTg     >  1:16627330/1‑137 (MQ=255)

                                                                                                                                      aCCGCCCGCGCGGCAAACGATCTGGCAGGCGCTGCCCTATTGGAGGCGTACGCCCCGTCAAATTATCATCACGCCACTGTCGTAATCTGCCGACCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt    >  1:5691643/1‑137 (MQ=255)

                                                                                                                                       ccgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc   >  1:8004168/1‑137 (MQ=255)

                                                                                                                                        cgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt  >  1:8824035/1‑137 (MQ=255)

                                                                                                                                        cgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt  >  1:8823997/1‑137 (MQ=255)

CGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGC‑GCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344172‑4344443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`