Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,889,6190AC29.4% 172.3 / 30.5 137intergenic (+65/‑88)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
Reads supporting (aligned to +/- strand):  ref base A (33/63);  new base C (7/33);  total (40/97)
Fisher's exact test for biased strand distribution p-value = 6.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 144 total aligned reads displayed.

GGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACC‑GGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGG  >  AE015451_phaM1/4889488‑4889755
                                                                                                                                     |                                                                                                                                             
ggAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGGGTGGCCGTGAACACCGGGCAAAACGGGGGAcacccaccaa                                                                                                                                           >  1:1530482/1‑135 (MQ=255)
ggAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGTCCCCGCACACC‑AGAAAAGCCAGTTCCAcccccccaa                                                                                                                                          >  2:14880332/1‑134 (MQ=255)
ggAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGAGGCCGGCCCCCCGCACC‑GGCAAAACCAGTTCCCTCCAccaaa                                                                                                                                          >  2:6778070/1‑134 (MQ=255)
  aGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACC‑GGCAAAGCCGGTGCCATCCACCGA‑Gtt                                                                                                                                       <  1:3912794/137‑1 (MQ=255)
         gCCGCTGACCACCACCGAAGCGTGCCTGGGCTTGCCGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACC‑GGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCt                                                                                                                               <  1:14781095/137‑1 (MQ=255)
                 ccaccaccGAGGTGGACCGGGGCTGGTGGAAAACCCACACCGCAACCT‑GAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCTCCTGCGAACACC‑GGCAAAGCCGGTGCCATCCCCCGA‑GTTG‑TCTGCTTCGCGGGc                                                                                                                       <  2:15940890/137‑1 (MQ=25)
                 ccaccaccGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGGTTTCCCGCGAAAAAC‑AGCACAGACAGGGACATCCCCCGA‑GTTT‑TGTTCTTCTCgggg                                                                                                                       >  2:16407975/1‑136 (MQ=255)
                    ccaccGAAGTGGGCCTGGGTTGGCGGAAAACCCACACCGCATCCT‑GGGAAACAACCTGGGACCCTTTTGGGCCGGGCCTTCCCGCGAACACC‑GGCAAATCCGGGGCCACCCCCCGG‑TTTG‑TTGGCTTCGCGGGCggg                                                                                                                    <  2:15983761/137‑3 (MQ=255)
                        cGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGATCGGCCGCGAACCAC‑AGAAAAGCCAGTGTCACCCCCCGA‑AGTG‑TCTTCCTCGCGGGGGAGgc‑‑cc                                                                                                              >  2:14959588/1‑136 (MQ=255)
                        cGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGAGGGGTGGCTCAGGAAAAAA‑GGAAAAAAAGGTGCCAGCCACCAA‑AATG‑GCTGCTTCCTGGGCgggggcc                                                                                                                >  1:15545692/1‑131 (MQ=255)
                               ggctgggggTGGCTGAAAGCCCACAACGCAGCCT‑GAGAAAAACTTGGGGACCTTGTGGGAGAGGTCCCTCCGTCGAACACT‑GGCAAATTCGGTGCCGTCCACCGA‑GTTG‑TCTGCTTCGCGGGCGACCC‑‑CGCTCCcac                                                                                                       <  2:13415246/132‑1 (MQ=255)
                                  cTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGATCGCCCCCCCACACC‑CACAAAACCAGTGTCACCCCCCCA‑GGTG‑TCTTTTTCTCCGGCGCGCC‑‑CCCTCCccc‑acg                                                                                                   >  2:4169174/1‑135 (MQ=255)
                                         ggtggAAAACCCACAAC‑CAAACTGGAGAAAAACCTCGGGACCTTGGGGGGGTGGGCTTGCCCGAAAACACG‑GGAAAGTTTGGTGCCACCCACCGG‑TTGG‑TTTGTTTTGTGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCaa                                                                                            <  2:10841930/133‑1 (MQ=255)
                                         ggtgaaaacCCCACAACGCACTCG‑GAGAAACAACTCGGGACCTTTTGGGAGTGGCCTCCCCTGCAAACACC‑GAAAAATCTGTTCCCCCCCCCCGG‑TTTT‑TCTTCTTCGTGGGCAACCC‑‑CGCTCCCAC‑AGGGGGCCaa                                                                                            <  2:8622144/132‑1 (MQ=255)
                                         ggagaaaacCCCAAAACGCAATTG‑GAGAAAAAATTTGGGACCTGGGGGGAGGGGGCTCACCAGAAAACACA‑GAAAAAGCTGGTGCCATCCACGGG‑GTTT‑TTTGTTTCGGGGACAACCC‑‑CGCTCCCAC‑AGGGGGCCaa                                                                                            <  2:9356593/132‑1 (MQ=255)
                                         ggaaaaaaaCCAACAACACAACCG‑GAGAAAAAACTCGGGACCTGGGGGGAGCGGGCTCCCCCGCAAACACG‑GGCAAATTGGGTGCCATCCACGGG‑GTTT‑TTGGTTTCGGGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCaa                                                                                            <  2:10819541/132‑1 (MQ=255)
                                             gAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGGTCTCCCTCGGACCCC‑CGCAAAAACAGTGTCCCCCCCCAA‑AGTG‑TTTTCTTCTCCGGCGGGCC‑‑CCCTCCCCCAAGGGGACCaaaac                                                                                         >  2:11888839/1‑135 (MQ=255)
                                             aaaaaCCAAAAACGCAACCG‑AAGAAACACCTAGGGACCTTGGGGGAGCGGCCTCACCAAAAAAAACA‑GAAAAAGCTGGTACCACCCACCGG‑GTTG‑TCGGCTTCGGGGACAACCC‑‑CGCTCCCAC‑AGGGGGCCAAAgct                                                                                        <  2:2045633/136‑1 (MQ=255)
                                              aaaccccaaaccccACTCC‑GAGAAACACCTTGGGACCTTGTGGGAGCGGTCTCTCCCGCAAACACC‑GGAAAAGCCGGTGCCCCCCACCGG‑TTTT‑TTTGTTTCGTGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAgctt                                                                                       <  2:15821525/137‑1 (MQ=255)
                                              aaacCCCACAACGAAGGTG‑GAGAAAAAACTTGGGACCTTGGGGGGGCGGGCCCGCCAGAAAACACC‑GGAAAAGCTGGTACCACCCA‑CGG‑GTTGTTCGGTTTCGTGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAgctt                                                                                       <  2:9252755/137‑1 (MQ=255)
                                                    caaaaCACACTCT‑AAGGAACAACTTGGAACCTGGGGGGAAGGGGCTCGCCCGAGAACACG‑GACAAAGCGGGTGCACTCCACCGA‑GTGG‑TCTGCTTCGCGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTga                                                                                 <  2:4557323/134‑1 (MQ=255)
                                                      ccacccAATCT‑GAGAAAAGGCTCGGGACCTGGGGGGAGCGGTCTCCCCAGAGAACATC‑GGAAACGCGGGTCCCATCCACCGG‑GTGT‑TCTGTTTCGGGGTAGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTgaga                                                                               <  2:1858479/132‑1 (MQ=255)
                                                      caACTCAATCT‑GAGAAACACCTGGGGACCTTGTGGGATCGGGCTCTCCGGCAAACACC‑GACAAAACTGGTGCCATCCACCGG‑TTTG‑TCTGCTTCGCGGACGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTgaga                                                                               <  2:13441254/137‑1 (MQ=255)
                                                      caACGCCCTCT‑GAGAAACACCTCGGAACCCTGTGGGAGTGGTTTCTCCCGAAAACACC‑GGAAAACCTGGTACCACCCACCGA‑TTGG‑TTTGCTTCGCGGGCAACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTgaga                                                                               <  2:2984556/137‑1 (MQ=255)
                                                      caACGCACTCG‑GAGGAACAATTTGGGACCTTGGGGGGGTGGGTTCTCCCGAGAACACC‑GAAAAATCTGGTGCCACCCCCCGG‑GTTG‑TTTTCTTCGCGGGCAACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTgaga                                                                               <  2:12376114/137‑1 (MQ=255)
                                                      aactgaaaTCT‑GAGAAACAACTCGGAACCTTGGGGGATCGGACTCGCCAGGAAACACC‑‑GAAAAACCGGGGCCCGCCAACTAGGTGG‑TTGGCTTTGTGGGGGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTgaga                                                                               <  2:3709651/131‑1 (MQ=255)
                                                        aCGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGTGCTCGCCCGCGAACACC‑GGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGata                                                                             <  1:9186479/137‑1 (MQ=255)
                                                            aaTCT‑GAAGAACAACTTGGGACCTGGTGGGAGGGGTCTTACCTACGAACACC‑GAAAAATCCGGTGCCACCCACCGG‑TTTG‑TCTGCTTAGTGGGAAAACC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATcac                                                                         <  2:14202402/137‑1 (MQ=255)
                                                                 ggAGGAACACTTCGGGACCTTTTGGGAGTGGGCTGGCCAGCGAAAACT‑GGAAAAGCTGGGGCCATCCACCGG‑GTTG‑TCTGTTTCGTGGGTGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAAtt                                                                     <  2:15405234/136‑1 (MQ=255)
                                                                   gggAACAATTTGGGCTCTTGTGGGGGTGGGCTCTCCCGCAAACACC‑GCCAAATCTGGTTCCATCCACCGG‑TTTT‑TCTGCTTCGCGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTAc                                                                   <  1:17418438/136‑1 (MQ=255)
                                                                   ggaaacaccTCGGGACCTTGTGGGGGTGGGCTCTCCCGAGAACACG‑GGCAAAGCTGGTTCCCTCCCCTGG‑TTTT‑TTTTTTTTGTGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTAc                                                                   <  2:15111742/134‑1 (MQ=255)
                                                                   aGGCACCACTCGGGACCCTTTGGGAGTGCTCTCGCCCGAGAACACC‑GGCAAAGCGGGTCCCCCCCACAGG‑TTTG‑TCTGTTTTGCGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTAc                                                                   <  2:11264442/137‑1 (MQ=255)
                                                                   aGGAACACCTGGGGACCTTGGGGGAGCGTGTTCTCCCGCAAACACT‑GAAAAAGCGGGTGCCACCCACCGG‑TTTG‑TTTGTTTTGCGGGAGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTAc                                                                   <  2:11213010/137‑1 (MQ=255)
                                                                               ggACCTTTTGGGAGCGTTCTCTCCCGAGAACATC‑GGCAAGTCTGGTTCCGTCCCCCGA‑TTTG‑TCTGCTCCGTGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAtt                                                       <  2:5237750/137‑1 (MQ=255)
                                                                                gACCTTGTGGGGGGGGTTCCGCCCGAGAACACT‑GGAAAGGCCGGTGCCCCCCCCGGG‑TTTG‑TTTTTTTCGCGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAttt                                                      <  2:15429122/137‑1 (MQ=255)
                                                                                gACCCTGTGGGAGCGGTTTCTCCCGAAAACCCC‑GACAAATCCGGTTCCCTCCCCCGG‑TTTT‑TCTTTTTCGGGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAttt                                                      <  2:2506441/137‑1 (MQ=255)
                                                                                aaCCTTGGGGGAGGGGCCTCACCAAAGAAAACC‑AGAAAAGCTGGGGCCATCCACTGA‑GTTG‑TTTGTTTCGAGGTCAACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAttt                                                      <  2:3889396/136‑1 (MQ=255)
                                                                                aaCCCTGTGGGAGCGGTCCCGCCTGCGAAAACC‑GGCAAAGCCGGTGCCATCCACCGA‑GTTT‑TCTGCTTCGTGGGCGGTCC‑‑CGCTCCCAC‑AGTGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAttt                                                      <  2:12130579/136‑1 (MQ=37)
                                                                                           gggggtcTCGCCCAAAAAAACA‑GGAAAATCGGGTTCCATCCCCCGG‑TTTG‑TTTGCTTCGTGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTaa                                           <  2:5656304/131‑1 (MQ=255)
                                                                                           agtggccTCCCCCGAAAACACC‑GGAAAATCCGTTTCCCCCCCCCGA‑GTTT‑TCTCCTCTACGTCCGAACC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTaa                                           <  1:11305931/131‑1 (MQ=255)
                                                                                               ggCTCGCCTGAAAACACA‑GGCAAAACCGGGCCCATCCACTGA‑GGTG‑TCTGCTTCGCGGGCGGACC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTGGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATc                                       <  2:9694325/137‑1 (MQ=255)
                                                                                               gccTCGCCCGCGAACACC‑GGAAACCCTGTTGCCCCCCCCCGA‑TTTG‑TTTTCTTTTTGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATc                                       <  2:12405726/135‑1 (MQ=255)
                                                                                                    gCCCGGAAACACC‑GGAAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTGGTGGGTGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGttt                                  <  2:16042328/137‑1 (MQ=255)
                                                                                                     cccgaaaaCACC‑GACAAAACCGGTGCCATCCCCGGG‑GTTG‑TTTGCTTTGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTc                                 <  2:869272/131‑1 (MQ=255)
                                                                                                      ccgaaaaCACC‑GGAAAAGCCGGTACCACCCACTGG‑GTTT‑TCTTCTTCGGGGGTGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCa                                <  2:3682033/132‑1 (MQ=255)
                                                                                                      ccGAGAACACC‑GACAAAGCTGGTGCCATCCCCTGG‑TTTG‑TCTGCTTCGCGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCa                                <  2:12154882/137‑1 (MQ=255)
                                                                                                       tgcgAACACT‑GAAAAACCCGGTGCCGTCCACTGA‑GTTG‑TCTGTTTTCCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGCTTCAt                               <  2:8425042/136‑1 (MQ=255)
                                                                                                            acacC‑GGAAAATCTGGTGCCATCCACTGG‑GTTG‑TTTGCTTCGTGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCa                          <  2:13891690/137‑1 (MQ=255)
                                                                                                             cacC‑GGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAc                         >  1:10022970/1‑137 (MQ=255)
                                                                                                              acC‑AGCAAAGACGGTGCCATCCACCAA‑GGTG‑TCTGCTTCGCGGGCGGACC‑‑CGCTCCCAC‑AAGGGGCCAAAACTTGCTTGAGATATCACAATTGCAAAGGGGTAACTTCTCATGGCCAAAACCGGTTTCATTGGCAcc                        >  2:4507868/1‑137 (MQ=255)
                                                                                                                  ggaaaaCCCGGTGCCCTCCCCCGA‑TTTT‑TCCTCTTTGCGGTTGACCC‑‑CGCTCCCAC‑ATGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                     <  2:213548/134‑1 (MQ=255)
                                                                                                                  ggCAAATCTGGTGCCATCCCCTGG‑TTTG‑TCTGCTTCGTGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                     <  2:2421094/137‑1 (MQ=255)
                                                                                                                  ggCAAAGCGGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGGTCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                     <  2:15939624/137‑1 (MQ=255)
                                                                                                                  ggCAAAGCCGGTGCCATCCACCGA‑GTTG‑TTTGCTTCGCGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCGTGCGTGAGATATCACAATTACAAAGAGGTAATTTCTCAGGGCTAAAATCGGTTTCATCGGCACCGGc                     <  2:11972466/137‑1 (MQ=255)
                                                                                                                  ggCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTACAATCGGTTTCATCGGCACCGGc                     >  1:3293683/1‑137 (MQ=255)
                                                                                                                  gacAAAGCTGTTGCCATCCACCGA‑GTTG‑TTTGCTTCGCGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                     <  2:8903263/135‑1 (MQ=255)
                                                                                                                  gacAAACCTGGTCCCCTCCCCCGT‑GTTT‑TTTTTTTCGCGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                     <  1:4560915/135‑1 (MQ=255)
                                                                                                                  gaaaaaGCCGTTACCCTCCACAGG‑TTTG‑TCGGTTTAGCGGGCAATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                     <  2:7344855/134‑1 (MQ=255)
                                                                                                                   gaaaaGCGGGTGCCATCCCCTGG‑GTTG‑TTTGTTTGGGGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGca                    <  2:6306735/135‑1 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  1:3817727/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  1:13151232/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  1:1367315/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  2:2753671/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  2:16982297/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  2:10582111/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  2:10582112/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAAGTCATTTCTCATGGCTACACTCGGTTTCATCGGCACCGGcat                   >  1:3656190/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCATCCACCGA‑GTTG‑TATGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  1:14085839/1‑137 (MQ=255)
                                                                                                                    cAAAGCCGGTGCCACCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAACTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                   >  1:11325186/1‑137 (MQ=255)
                                                                                                                      acaccGGTCCCATCCCCCGG‑TTTT‑CCTGCTCATCGGATGAGCC‑‑CACTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatca                 <  1:2737675/134‑1 (MQ=255)
                                                                                                                      aaGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAACGCTTGCTTGAGATATCACAATTACAAAGAGGTAATCTCTCATTGCTAAAATCGGTTTCATCGGCACAGGcatca                 >  2:14333833/1‑137 (MQ=255)
                                                                                                                       atctgttGCCCTCCCCGGA‑GTTG‑TTTGCTTCAGGGGCAACCC‑‑CTCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAACGACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatcat                <  2:11356578/131‑1 (MQ=255)
                                                                                                                        tctgttGCCATCCCCCGG‑TTTT‑TTTGCTTAGCGTGCGGACC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTACTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg               <  2:8653852/132‑1 (MQ=255)
                                                                                                                        tctggTGCAATCCACTGA‑GTTT‑TCTGCTCCGTGGGCAAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg               <  2:8653814/134‑1 (MQ=255)
                                                                                                                        gctggTGCCATCCACCGG‑GTTT‑TTGGCTTCGTGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg               <  2:13929532/134‑1 (MQ=255)
                                                                                                                        ccggttGCCATCCCCCGG‑TTTT‑TTTTTTTCGCGGACACCCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg               <  1:10268236/132‑1 (MQ=255)
                                                                                                                        actggTTCCATCTCCCAA‑TTTG‑TTTTCTTAGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg               <  2:12693508/134‑1 (MQ=255)
                                                                                                                          cGGTGCCATCCACCGG‑TTTT‑CCTGCTTCGAGTGTGAGCCCGCGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg               <  2:9872558/137‑1 (MQ=255)
                                                                                                                          cGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATggg             >  1:6425490/1‑137 (MQ=255)
                                                                                                                          cGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATggg             >  1:6425491/1‑137 (MQ=255)
                                                                                                                          cGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATggg             >  1:9374620/1‑137 (MQ=255)
                                                                                                                           ggTGCCGTCCCCTGG‑TTTG‑TCGGTTTGGGGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGc            <  1:1655353/137‑1 (MQ=255)
                                                                                                                           ggTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCg                             <  2:5507994/120‑1 (MQ=255)
                                                                                                                           ggTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCg                             >  1:5507994/1‑120 (MQ=255)
                                                                                                                            ttGCCATCCACCGA‑GTTG‑TCTGTTTCGTGGGCAACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa           <  1:13900318/136‑1 (MQ=255)
                                                                                                                            gTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa           >  2:4226236/1‑137 (MQ=255)
                                                                                                                              gCCATCCACCGG‑TTTT‑TTTGTTTCGTGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGGGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAg         <  2:16828139/137‑1 (MQ=255)
                                                                                                                               ccATCCCCGGG‑GTTG‑TCTGTTTCGTGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc        <  1:10085962/137‑1 (MQ=255)
                                                                                                                               ccATCCCCCGA‑TTTT‑TTTGCTCCGCGGGAGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACACTTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATAGGCACCGGCATCATGGGCAAGc        <  2:1167024/137‑1 (MQ=255)
                                                                                                                               ccATCCCCCGA‑TTTT‑TTTATTTCGCGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc        <  2:6509761/137‑1 (MQ=255)
                                                                                                                               ccATCCCCCGA‑TTTG‑TTTGCTTCGCGGGCGATCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc        <  2:15565399/137‑1 (MQ=255)
                                                                                                                               ccATCCCCCGA‑GTTT‑TTTTCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc        <  2:8199727/137‑1 (MQ=255)
                                                                                                                               ccATCCACCGG‑TTTT‑TTTTTTTCGGGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGATGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAgt        <  1:16645905/137‑2 (MQ=255)
                                                                                                                               ccATCCACCGG‑GTTG‑TCTGCTTCGCGGGCGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc        <  2:8199728/137‑1 (MQ=255)
                                                                                                                               ccATCCACCGG‑GTTG‑TCGGCTTCGTGGACGACCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATAGGCACCGGCATCATGGGCAAGc        <  2:1166980/137‑1 (MQ=255)
                                                                                                                               ccATCCACCGA‑GTTT‑TCGGTTTCGGGGGAAAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc        <  2:16398829/137‑1 (MQ=255)
                                                                                                                                cATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc       >  1:7453873/1‑137 (MQ=255)
                                                                                                                                     aCCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg  >  1:5743194/1‑137 (MQ=255)
                                                                                                                                     aCCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg  >  2:15010016/1‑137 (MQ=255)
                                                                                                                                     aCCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg  >  2:9097302/1‑137 (MQ=255)
                                                                                                                                     |                                                                                                                                             
GGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCT‑GAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACC‑GGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCAC‑AGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGG  >  AE015451_phaM1/4889488‑4889755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑