Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,889,6440GC38.5% 326.6 / 131.8 229intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
Reads supporting (aligned to +/- strand):  ref base G (89/29);  new base C (0/74);  total (91/138)
Fisher's exact test for biased strand distribution p-value = 1.50e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 229 total aligned reads displayed.

CGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGG  >  AE015451_phaM1/4889512‑4889777
                                                                                                                                      |                                                                                                                                       
       ggctgggggTGGCTGAAAGCCCACAACGCAGCCTGAGAAAAACTTGGGGACCTTGTGGGAGAGGTCCCTCCGTCGAACACTGGCAAATTCGGTGCCGTCCACCGA‑GTTG‑TCTGCTTCGCGGGCGACCC‑‑CGCTCCcac                                                                                                                            <  2:13415246/132‑1 (MQ=255)
                gggcggAAAACCCACAATGAACCTTGGGGAACACCTTTGGACCCTGTGGGAGCGTGCTCGCCAGCGAACACCGACAAATCCGTTCCCACCCACCGG‑TTTT‑TCGGTTTCGTGGGCGACCC‑‑CGCTCCCACAGGGGGCCa                                                                                                                   <  2:16683452/136‑1 (MQ=255)
                 ggtgaaaacCCCACAACGCACTCGGAGAAACAACTCGGGACCTTTTGGGAGTGGCCTCCCCTGCAAACACCGAAAAATCTGTTCCCCCCCCCCGG‑TTTT‑TCTTCTTCGTGGGCAACCC‑‑CGCTCCCACAGGGGGCCaa                                                                                                                  <  2:8622144/132‑1 (MQ=255)
                 ggaaaaaaaCCAACAACACAACCGGAGAAAAAACTCGGGACCTGGGGGGAGCGGGCTCCCCCGCAAACACGGGCAAATTGGGTGCCATCCACGGG‑GTTT‑TTGGTTTCGGGGGCGACCC‑‑CGCTCCCACAGGGGGCCaa                                                                                                                  <  2:10819541/132‑1 (MQ=255)
                     aaaaaCCAAAAACGCAACCGAAGAAACACCTAGGGACCTTGGGGGAGCGGCCTCACCAAAAAAAACAGAAAAAGCTGGTACCACCCACCGG‑GTTG‑TCGGCTTCGGGGACAACCC‑‑CGCTCCCACAGGGGGCCAAAgct                                                                                                              <  2:2045633/136‑1 (MQ=255)
                      aaaccccaaaccccACTCCGAGAAACACCTTGGGACCTTGTGGGAGCGGTCTCTCCCGCAAACACCGGAAAAGCCGGTGCCCCCCACCGG‑TTTT‑TTTGTTTCGTGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAgctt                                                                                                             <  2:15821525/137‑1 (MQ=255)
                      aaacCCCACAACGAAGGTGGAGAAAAAACTTGGGACCTTGGGGGGGCGGGCCCGCCAGAAAACACCGGAAAAGCTGGTACCACCCA‑CGG‑GTTGTTCGGTTTCGTGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAgctt                                                                                                             <  2:9252755/137‑1 (MQ=255)
                      aaaaCCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGGTTGCCCATGCACACCCGCCAAACCCGTTTCCCCCCCCCA‑GTTG‑TTTTTCTCTCGGGCGCGCC‑‑CTCCCCCACCAGGGGAAAAAgact                                                                                                             >  2:5121978/1‑134 (MQ=255)
                              ccacccAATCTGAGAAAAGGCTCGGGACCTGGGGGGAGCGGTCTCCCCAGAGAACATCGGAAACGCGGGTCCCATCCACCGG‑GTGT‑TCTGTTTCGGGGTAGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                                     <  2:1858479/132‑1 (MQ=255)
                              caACGCACTCGGAGGAACAATTTGGGACCTTGGGGGGGTGGGTTCTCCCGAGAACACCGAAAAATCTGGTGCCACCCCCCGG‑GTTG‑TTTTCTTCGCGGGCAACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                                     <  2:12376114/137‑1 (MQ=255)
                              aactgaaaTCTGAGAAACAACTCGGAACCTTGGGGGATCGGACTCGCCAGGAAACACC‑GAAAAACCGGGGCCCGCCAACTAGGTGG‑TTGGCTTTGTGGGGGATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                                     <  2:3709651/131‑1 (MQ=255)
                              aaaCGCAATCTGAGAAAAAACTCGGAACCTTTTGGGAGCGGGCTTGCCCGCAAACACCGGAAAACCTGGTGCCACCCACCGG‑TTTG‑TTTTCTTCGCGGGCAACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                                     <  2:13120628/136‑1 (MQ=18)
                              aaaCGCAATCGGAGGAACAACTTGGGACTTTGGGGGAGTGGGCTCGCCCGAGAACCCAAACAAAGTTGTTCCCCTCCCCTGA‑TTTT‑TTGGTTTCGCGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                                     <  2:13470055/136‑1 (MQ=255)
                                aCGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGTGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGata                                                                                                   <  1:9186479/137‑1 (MQ=255)
                                    aaTCTGAAGAACAACTTGGGACCTGGTGGGAGGGGTCTTACCTACGAACACCGAAAAATCCGGTGCCACCCACCGG‑TTTG‑TCTGCTTAGTGGGAAAACC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATcac                                                                                               <  2:14202402/137‑1 (MQ=255)
                                        tGAGAAACACCTTGGAACCCTTTGGGAGTGGTCTCCCCCGTGAACCCCGACAAACCTGGTGCCATCCCCCGG‑TTTG‑TCTGCTTCGCGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAtt                                                                                           <  2:15405235/137‑1 (MQ=255)
                                          gggAACAATTTGGGCTCTTGTGGGGGTGGGCTCTCCCGCAAACACCGCCAAATCTGGTTCCATCCACCGG‑TTTT‑TCTGCTTCGCGGGCGATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTAc                                                                                         <  1:17418438/136‑1 (MQ=255)
                                          gggAAAAATTCGGGACCCTGTGTGAGCGGGCTTGCCCGCAAACACTGACAAATCTGGTGCCATCCCCCGA‑TTTG‑TCTTCTTCGCGGCCGCTCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTAc                                                                                         <  2:16390188/136‑1 (MQ=255)
                                                       aaCCTTGGGGGAGGGGCCTCACCAAAGAAAACCAGAAAAGCTGGGGCCATCCACTGA‑GTTG‑TTTGTTTCGAGGTCAACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAttt                                                                            <  2:3889396/136‑1 (MQ=255)
                                                                           gCCCGGAAACACCGGAAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTGGTGGGTGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGttt                                                        <  2:16042328/137‑1 (MQ=255)
                                                                            cccgaaaaCACCGACAAAACCGGTGCCATCCCCGGG‑GTTG‑TTTGCTTTGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTc                                                       <  2:869272/131‑1 (MQ=255)
                                                                            cccGAGAACACCGAAAAATCTGGTGCCACCCACAGA‑GTTG‑TCTGTTTCGCGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTc                                                       <  2:2145220/137‑1 (MQ=255)
                                                                                   acacCGGAAAATCTGGTGCCATCCACTGG‑GTTG‑TTTGCTTCGTGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCa                                                <  2:13891690/137‑1 (MQ=255)
                                                                                    cacCGGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAc                                               >  1:10022970/1‑137 (MQ=255)
                                                                                     acCAGCAAAGACGGTGCCATCCACCAA‑GGTG‑TCTGCTTCGCGGGCGGACC‑‑CGCTCCCACAAGGGGCCAAAACTTGCTTGAGATATCACAATTGCAAAGGGGTAACTTCTCATGGCCAAAACCGGTTTCATTGGCAcc                                              >  2:4507868/1‑137 (MQ=255)
                                                                                        ggCAAAGCCGGTGCCATCCACCGA‑GTTG‑TTTGCTTCGCGGGCGATCC‑‑CGCTCCCACAGGGGGCCAAAGCGTGCGTGAGATATCACAATTACAAAGAGGTAATTTCTCAGGGCTAAAATCGGTTTCATCGGCACCGGc                                           <  2:11972466/137‑1 (MQ=255)
                                                                                        gacAAACCTGGTCCCCTCCCCCGT‑GTTT‑TTTTTTTCGCGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                           <  1:4560915/135‑1 (MQ=255)
                                                                                        gaaaaaGCCGTTACCCTCCACAGG‑TTTG‑TCGGTTTAGCGGGCAATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                           <  2:7344855/134‑1 (MQ=255)
                                                                                         gaaaaGCGGGTTCCACCCACGGG‑TTTG‑TTTGTTTCGTGGGCAACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGca                                          <  2:17119296/135‑1 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  1:1367315/1‑137 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  2:958359/1‑137 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  1:13151232/1‑137 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  2:10582112/1‑137 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  2:5560568/1‑137 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  1:3817755/1‑137 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  1:16144719/1‑137 (MQ=255)
                                                                                          cAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                         >  2:2753671/1‑137 (MQ=255)
                                                                                            acaccGGTCCCATCCCCCGG‑TTTT‑CCTGCTCATCGGATGAGCC‑‑CACTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatca                                       <  1:2737675/134‑1 (MQ=255)
                                                                                             gtctggTGCCATCCCCTGG‑TTTT‑TCTGTTTCGTGGGCGCCCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatcat                                      <  1:606636/133‑1 (MQ=255)
                                                                                             atctgttGCCCTCCCCGGA‑GTTG‑TTTGCTTCAGGGGCAACCC‑‑CTCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAACGACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatcat                                      <  2:11356578/131‑1 (MQ=255)
                                                                                              tctggTGCAATCCACTGA‑GTTT‑TCTGCTCCGTGGGCAAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                     <  2:8653814/134‑1 (MQ=255)
                                                                                              gttggTGCCATCAATTGG‑GTTG‑TTTGTTTCGTGGGAGATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                     <  2:14441501/134‑1 (MQ=255)
                                                                                              gctggTGCCATCCACCGG‑GTTT‑TTGGCTTCGTGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                     <  2:13929532/134‑1 (MQ=255)
                                                                                                cGGTGCCATCCACCGG‑TTTT‑CCTGCTTCGAGTGTGAGCCCGCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                     <  2:9872558/137‑1 (MQ=255)
                                                                                                  ttGCCATCCACCGA‑GTTG‑TCTGTTTCGTGGGCAACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa                                 <  1:13900318/136‑1 (MQ=255)
                                                                                                  gTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa                                 >  2:4226236/1‑137 (MQ=255)
                                                                                                     ccATCCCCCTA‑TTTT‑TCTGCTTCGTGGGCGATCC‑‑CCCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                              <  2:15030536/137‑1 (MQ=255)
                                                                                                     ccATCCCCCGA‑TTTT‑TTTGCTCCGCGGGAGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACACTTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATAGGCACCGGCATCATGGGCAAGc                              <  2:1167024/137‑1 (MQ=255)
                                                                                                     ccATCCCCCGA‑TTTG‑TTTGCTTCGCGGGCGATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                              <  2:15565399/137‑1 (MQ=255)
                                                                                                     ccATCCCCCGA‑GTTT‑TTTTCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                              <  2:8199727/137‑1 (MQ=255)
                                                                                                     ccATCCACGGG‑GTTT‑TCTTTTTTGTGGTCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                              <  2:6509812/137‑1 (MQ=255)
                                                                                                     ccATCCACCGG‑GTTG‑TTGGTTTTGTGGGAGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                              <  2:8421932/137‑1 (MQ=255)
                                                                                                      cATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                             >  1:11153547/1‑137 (MQ=255)
                                                                                                      aaTCCAACGA‑GTTG‑TATGCTTCGCGGGCGAGCC‑‑CGCTCCCAAAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTGAAGTCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                             >  2:1100256/2‑137 (MQ=255)
                                                                                                           aCCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                        >  2:9097302/1‑137 (MQ=255)
                                                                                                           aCCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                        >  2:15010016/1‑137 (MQ=255)
                                                                                                            ccGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGc                       >  2:7972697/1‑137 (MQ=255)
                                                                                                                 ttgg‑TCTGTTTCGGGGGAGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                   <  2:1213722/134‑1 (MQ=255)
                                                                                                                 ttgg‑TCTGCTTAGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAGTTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                   <  2:3265274/134‑1 (MQ=255)
                                                                                                                 gTTT‑TCTGCTCCGCGGACAACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                   <  1:3924673/137‑1 (MQ=255)
                                                                                                                 gTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                   >  2:12678571/1‑137 (MQ=255)
                                                                                                                   tG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGTTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                 >  2:16323052/1‑137 (MQ=255)
                                                                                                                   tG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                 >  1:10537281/1‑137 (MQ=255)
                                                                                                                   tG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                 >  2:14177294/1‑137 (MQ=255)
                                                                                                                   tG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                 >  2:10532995/1‑137 (MQ=255)
                                                                                                                   tG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                 >  1:7669506/1‑137 (MQ=255)
                                                                                                                       cTGCTTTGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCt              <  1:11325024/137‑1 (MQ=255)
                                                                                                                       cTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCt              >  2:16835934/1‑137 (MQ=255)
                                                                                                                        tGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg             >  1:4752892/1‑137 (MQ=255)
                                                                                                                         tcTTCGCGGGCGATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc            <  2:6410077/136‑1 (MQ=255)
                                                                                                                         gCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc            >  2:5973147/1‑137 (MQ=255)
                                                                                                                         gCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc            >  2:9063710/1‑137 (MQ=255)
                                                                                                                         gCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc            >  2:10029731/1‑137 (MQ=255)
                                                                                                                         gCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc            >  2:11247268/1‑137 (MQ=255)
                                                                                                                           ttCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaa          >  2:12235373/1‑137 (MQ=255)
                                                                                                                            tggtgggCCATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa         <  1:11806473/133‑1 (MQ=255)
                                                                                                                            tCGCGGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa         <  2:17384873/137‑1 (MQ=255)
                                                                                                                             tgagggCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  1:7087956/134‑1 (MQ=255)
                                                                                                                             ggggggCAACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  2:887437/134‑1 (MQ=255)
                                                                                                                             cgcgGGCGATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  1:7887796/137‑1 (MQ=255)
                                                                                                                             cgcgGGCGATCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  2:11838678/137‑1 (MQ=255)
                                                                                                                             cgcgGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  2:3654633/137‑1 (MQ=255)
                                                                                                                             cgcgGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  1:12543255/137‑1 (MQ=255)
                                                                                                                             cgcgGGCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  1:10210432/137‑1 (MQ=255)
                                                                                                                             cgcgGGCACACC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  1:11557959/137‑1 (MQ=255)
                                                                                                                             cgcgGGCAAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  2:11133288/137‑1 (MQ=255)
                                                                                                                             cgcgGGCAAGCC‑‑CACTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGCTTCATCGGCACCGGCATCATGGACAAGCCCATGGCTCAGAACCTGCaaaa        <  2:2524042/137‑1 (MQ=255)
                                                                                                                             cgcgGACGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  1:15773231/137‑1 (MQ=255)
                                                                                                                             cgcgGACGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa        <  2:15622400/137‑1 (MQ=255)
                                                                                                                              gcgGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAg       >  1:17311285/1‑137 (MQ=255)
                                                                                                                               tgggCGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAgg      <  1:17097704/136‑1 (MQ=255)
                                                                                                                                gggCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc     >  1:2855954/1‑137 (MQ=255)
                                                                                                                                gggCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc     >  1:5664434/1‑137 (MQ=255)
                                                                                                                                gggCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc     >  1:723903/1‑137 (MQ=255)
                                                                                                                                gggCGAGCC‑‑CGCTCCAACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCCGCAAAAGGc     >  1:5299388/1‑137 (MQ=255)
                                                                                                                                  gCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg   >  2:4208689/1‑137 (MQ=255)
                                                                                                                                  gCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg   >  1:2161803/1‑137 (MQ=255)
                                                                                                                                  gCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg   <  1:3433957/137‑1 (MQ=255)
                                                                                                                                  acGACCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg   <  1:6442377/136‑1 (MQ=255)
                                                                                                                                   cGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAgg  >  1:15024231/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGA‑GTTG‑TCTGCTTCGCGGGCGAGCC‑‑CGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGG  >  AE015451_phaM1/4889512‑4889777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑