BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	499,203	A→G	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA) → / → PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	499,203	0	A	G	100.0%	725.9 / NA	188	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA)/PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/157); total (31/157)

Only 100 of 189 total aligned reads displayed.

ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499070‑499330

  ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGGGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                               <  1:3854377/137‑1 (MQ=255)

   cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCggggacg                                                                                                                              >  2:2848559/1‑133 (MQ=255)

   cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg                                                                                                                              <  1:8052421/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGGGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:2245492/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:8498533/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:16126907/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:14234609/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:17111622/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:16962360/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:9547063/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:17111634/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:7580618/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:14448942/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:1344119/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:4823061/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:1105593/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:4150175/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGACCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:336100/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGNGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:17450582/137‑1 (MQ=255)

           aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGaa                                                                                                                      <  1:12807948/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:14752509/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:5465701/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:12281071/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  2:95772/137‑1 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATTCCCTCGaaacgg                                                                                                                  >  1:12232440/1‑133 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑agg                                                                                                                 >  2:15970625/1‑137 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCCaaaagg                                                                                                                  >  1:10808906/1‑133 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGTCCGCGaaaagg                                                                                                                  >  1:1574647/1‑133 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGCCcgcggaaaag                                                                                                                  >  1:13544754/1‑131 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGACGCACGCGAAcaag                                                                                                                  >  1:7133809/1‑134 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACGAAC‑agg                                                                                                                 >  2:1883361/1‑137 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  2:11892847/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:4749416/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  2:12348595/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:10260202/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:15466429/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:17216561/137‑1 (MQ=255)

                  aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                              <  1:2741013/137‑1 (MQ=255)

                  aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                              <  1:11871867/137‑1 (MQ=255)

                  aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                              <  1:12374535/137‑1 (MQ=255)

                   tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctg                                                                                                             <  2:811891/137‑1 (MQ=255)

                   tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAACGCCCGCGAAC‑Gggactg                                                                                                             >  2:10565852/1‑137 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGGGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  1:1730343/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  1:596380/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  2:592727/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  1:4476012/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCACGAAA‑Aggcccgga                                                                                                           >  2:12556233/1‑136 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCACAAAC‑Ggggctggg                                                                                                           >  1:17293956/1‑136 (MQ=255)

                        aatttGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:13924252/134‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGTTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctgacct‑g                                                                                                       <  1:15449510/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:7778738/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:10455945/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:6743325/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:5647668/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:5578782/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:5144807/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:4728459/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:16713617/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:6785124/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:15067040/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:4583168/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:8752510/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:11805484/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:23661/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:16907494/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCGGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:499052/137‑1 (MQ=255)

                        aaGTGGAAAGTAAACTTGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACACGCTGATCTGAGTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATTTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:7769362/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCct‑gct                                                                                                     <  2:14107632/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCct‑gct                                                                                                     <  2:6662830/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCAAGAAC‑AGGACTGGCct‑gct                                                                                                     >  1:7789180/1‑137 (MQ=255)

                          gTTGAAAGTGAACCCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCct‑gct                                                                                                     <  1:3578909/137‑1 (MQ=255)

                            tGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGa                                                                                                   <  1:11264331/137‑1 (MQ=255)

                                  tGAACTCGCTGTAGGAGCGGGTGAAGGACGCCATGGAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGTGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTa                                                                                             <  1:11417717/137‑1 (MQ=255)

                                  tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTa                                                                                             <  2:7129498/137‑1 (MQ=255)

                                  tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTa                                                                                             <  2:11551610/137‑1 (MQ=255)

                                            ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCTGCCCGCTAAC‑AGGACTTGCCT‑GTTGGTCTCCCTGGATGAGcc                                                                                   >  2:4927698/1‑137 (MQ=255)

                                              cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTTGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCgg                                                                                 <  2:7503247/137‑1 (MQ=255)

                                              cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCgg                                                                                 <  1:12108631/137‑1 (MQ=255)

                                              cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCgg                                                                                 <  2:5526179/137‑1 (MQ=255)

                                                   cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑Cctct                                                                           <  1:4369543/137‑1 (MQ=255)

                                                   cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑Cctct                                                                           <  2:13599699/137‑1 (MQ=255)

                                                   cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑Cctct                                                                           <  1:231886/137‑1 (MQ=255)

                                                             aCGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCAt                                                                 <  2:15561256/137‑1 (MQ=255)

                                                                      aGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑AAGACCGGCCT‑GCTGGTCTCTCCTGCTGAGCCGG‑CCTCTTCTCTGGCCTGtccccccc                                                        >  2:3663462/1‑132 (MQ=255)

                                                                      aGCACAAGCTGACCTGGTTGCGACATACGGGTTCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCGGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGcgaa                                                        <  2:5741736/137‑4 (MQ=255)

                                                                             gCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACGAAC‑AGGACCCGCCC‑GCTGGTTTCTCTGGATGAGACAG‑CCCCCTCACTGGCACGCCCCCTccccaaagg                                                 >  2:12303462/1‑131 (MQ=255)

                                                                                gatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCTAAC‑AGGCCTTGCCT‑TCTGGTTTCTCTGGCTTAGTCAG‑CCTCTTCTCGGGCACGCCCTCTCCCCCCAGTTTc                                              >  2:16979820/1‑137 (MQ=255)

                                                                                    tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGTCCACGAAC‑AGGCCTGGACC‑TCTGGTCTCTCTTGCTGGGCCGGACCCCTTCCCGGG‑ACGTCCCCTCCCCCAAGttttcttt                                          >  2:11862622/1‑132 (MQ=255)

                                                                                               tACGGGGACCCGAACTTTGCCGAATCCTTTGTGGGAGCGGACGTTCCCGAGAAT‑GGCCTTGGCTT‑GCTGATCTTTATGTTTGAGCTGT‑CTTTTTGGTGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:10110158/137‑1 (MQ=255)

                                                                                               ggtgggTGCTGGAAATTTTCCGAAGCTTGGTTGGGAGAGGGCGTGCCCGAGAAG‑GGGCCGGTCCT‑GAGGCTTTCCAGGGTGGCCCCGC‑CCTCTTCGGGGGCGTGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:16596083/134‑1 (MQ=255)

                                                                                               gactggTGCCCGACACTTGCTAAAATCCATGTGGAGGCGGGCATTCAAGAAAAC‑GGG‑CTGGCTTGGCTGATGCCTGTTACTAACCCTT‑CCTCTCCGAGGTCATGCCCGCGCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:7814679/133‑1 (MQ=255)

                                                                                                             ttttGCCGAGGCTTATGTGGGAGCGGGCCTTCCCGCGAAC‑GGGCTTGTCTT‑GTTGCTCTCCATGGCTAATCCTC‑CTTTTTCGCGGGCATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                 <  2:14727719/135‑1 (MQ=255)

                                                                                                             atttGTCGAAGCCTAGTGGGGAGGGGGTGTGCCCGAGAAG‑GGGCTTGGCCT‑GTTGGTCCTGGTGTCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                 <  2:16328086/135‑1 (MQ=19)

                                                                                                             atttGCGGAGGCTTGTGGGGGAGGGGGCATGCTCGAGAAG‑GGGTTTGGCCT‑GCGGATCTCTGTGTTGGATCCTT‑CTTCTTCGCGGGCGTTCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                 <  2:4268867/135‑1 (MQ=255)

                                                                                                               tttctgAAGCCTGTGGGGGAGCGGGCGTGCCCG‑GAACGGGGCCGGGCCT‑GTGGGTCTCCATGTCTGAGCTGC‑CCTCTTCGTGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg               <  2:17053229/132‑1 (MQ=19)

                                                                                                               ttGCCGAACCTTGGGGGGGAGGGGGCGTGCCCGAGAAT‑GGCCTTGTCTG‑GTTGATTTCGCTGGTTGGGCTGT‑CCTTTTCGTGGGCCTCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg               <  2:10842989/137‑1 (MQ=255)

                                                                                                                          tgtgtgGGAGAGGGCAGCCCCGAAAAC‑GGGCCTGGCCT‑GCTGCTCTCTGTGGTGGACCTGC‑CCTCTTTTTGGGCCTTCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa    <  2:1440197/135‑1 (MQ=255)

                                                                                                                            tgggggAGGGGGCGTGCCCGAGAAT‑GGGTCTGGTTT‑GCGGATTTTTGTGTCGAAGCTGC‑CTTCTTCGTGGCCCTCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  1:14924886/134‑1 (MQ=255)

                                                                                                                            tgggggAGCGGGTATGCCCGAGGAT‑GTGCCTGTTTT‑GTTGATCTCGGTTGCTGAGCCGC‑CTTCTGTGCGGGCATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  1:3234471/134‑1 (MQ=255)

                                                                                                                            tgggggAGCGGCCGTCCCCGCAAAT‑GGCCCTTTCCT‑GCTGCTCTTTATGGCTGCTCCGT‑CTTCTTCGCGGACATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  1:16247726/134‑1 (MQ=255)

ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499070‑499330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`