Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 539,294 A→C T558P (ACC→CCC)  rpoB CDS (DNA‑directed RNA polymerase subunit beta) →

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1539,2940AC100.0% 2343.2 / NA 578T558P (ACC→CCC) rpoB CDS (DNA‑directed RNA polymerase subunit beta)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (288/290);  total (288/290)

Only 100 of 583 total aligned reads displayed.

CAGCCAGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAG  >  AE015451_phaM1/539161‑539426
                                                                                                                                     |                                                                                                                                    
 agccagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCa                                                                                                                                  <  2:8202075/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTa                                                                                                                               <  2:6108270/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTa                                                                                                                               <  1:14395369/137‑1 (MQ=255)
    cagcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTa                                                                                                                               <  1:8760531/137‑1 (MQ=255)
     agcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTAt                                                                                                                              >  2:17489870/1‑137 (MQ=255)
     agcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTAt                                                                                                                              >  1:12414912/1‑137 (MQ=255)
     agcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTAt                                                                                                                              <  1:7938837/137‑1 (MQ=255)
        tGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGc                                                                                                                           <  1:15440580/137‑1 (MQ=255)
              aGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGTGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCgtgtg                                                                                                                     <  1:9831914/137‑1 (MQ=255)
               gTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCgtgtgt                                                                                                                    <  2:2532886/137‑1 (MQ=255)
                  cATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGcc                                                                                                                 >  1:11540335/1‑137 (MQ=255)
                    tGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCg                                                                                                               <  1:2477733/137‑1 (MQ=255)
                      gACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGAt                                                                                                             <  2:3695257/137‑1 (MQ=255)
                      gACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGAt                                                                                                             <  2:6837238/137‑1 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                            >  1:12078414/1‑137 (MQ=255)
                       aCCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATc                                                                                                            >  1:10427425/1‑137 (MQ=255)
                       aCCAGAAAAACCCTATCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGAGGTCCGACCCGTGAGTGTGCCGGATTCGAAGTCAGTGACGTACACCCGCACCACGATGGCAGTGTGTGACCGAta                                                                                                            >  2:16315795/1‑136 (MQ=255)
                        ccAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCg                                                                                                           >  1:12922045/1‑137 (MQ=255)
                             acaacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAcc                                                                                                      >  2:4254585/1‑137 (MQ=255)
                              caacCCTCTCTCTGAGATTACCCACAAGCGCCGCGCCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTACCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAccc                                                                                                     <  2:12283342/137‑1 (MQ=255)
                              caacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAccc                                                                                                     <  2:13659498/137‑1 (MQ=255)
                              caacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAccc                                                                                                     <  2:11945139/137‑1 (MQ=255)
                               aacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTTAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAcccc                                                                                                    >  1:13626217/1‑137 (MQ=255)
                               aacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAcccc                                                                                                    >  1:9621844/1‑137 (MQ=255)
                               aacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAcccc                                                                                                    >  1:543549/1‑137 (MQ=255)
                               aacCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAcccc                                                                                                    >  1:5052564/1‑137 (MQ=255)
                                   ctctctCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGAccc                                                                                                     >  1:8533320/1‑132 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAg                                                                                               >  2:15919278/1‑137 (MQ=255)
                                    tctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCATGAAg                                                                                               >  1:2797248/1‑137 (MQ=255)
                                     ctctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCTGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAgg                                                                                              <  2:7691572/137‑1 (MQ=255)
                                      tctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGt                                                                                             <  1:1790399/137‑1 (MQ=255)
                                      tctcGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGt                                                                                             >  1:13068431/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                          >  2:11112267/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                          >  2:12091656/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCg                                                                                          >  2:15165262/1‑137 (MQ=255)
                                         cGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTACCCGATCGAGACCCCTGAAGGTCCg                                                                                          >  2:6376340/1‑137 (MQ=255)
                                             gaTTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACa                                                                                      <  2:1837666/137‑1 (MQ=255)
                                              aTTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACAt                                                                                     >  1:15900912/1‑137 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                    <  2:1551129/137‑1 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                    <  2:7066506/137‑1 (MQ=255)
                                               ttACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc                                                                                    >  2:16744301/1‑137 (MQ=255)
                                                tACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCg                                                                                   >  1:15611258/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                >  2:5321859/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                >  1:12566662/1‑137 (MQ=255)
                                                   ccACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTc                                                                                >  2:8744496/1‑137 (MQ=255)
                                                     acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGc                                                                                                                  >  2:14070796/1‑101 (MQ=255)
                                                     acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGc                                                                                                                  <  1:14070796/101‑1 (MQ=255)
                                                     acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTg                                                                              >  1:9254347/1‑137 (MQ=255)
                                                     acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTg                                                                              >  2:5097919/1‑137 (MQ=255)
                                                     acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTg                                                                              >  2:2725042/1‑137 (MQ=255)
                                                          cgccgcGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTACGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCATGAAGGTCCGAACATCGGTCTGATCaa                                                                         >  2:15771691/1‑137 (MQ=255)
                                                           gccgcGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAAc                                                                        <  1:14784793/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTTCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  2:3902299/137‑1 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    >  2:11706515/1‑137 (MQ=255)
                                                               cgTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc                                                                    <  1:3223565/137‑1 (MQ=255)
                                                                    ccGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGACTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTggcg                                                               <  2:10130193/137‑1 (MQ=255)
                                                                         cTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTa                                                          <  1:10671241/137‑1 (MQ=255)
                                                                         cTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTa                                                          <  2:2344545/137‑1 (MQ=255)
                                                                         cTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTa                                                          <  1:5544579/137‑1 (MQ=255)
                                                                         cTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTa                                                          <  1:74787/137‑1 (MQ=255)
                                                                          tCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTAt                                                         >  2:5708261/1‑137 (MQ=255)
                                                                             gCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGCTCGACTCCCTGGCGGCCTATGcc                                                      >  2:17187332/1‑137 (MQ=255)
                                                                              cccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGccc                                                     <  1:3021131/137‑1 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                    >  1:16818326/1‑137 (MQ=255)
                                                                               ccTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCg                                                    >  1:2026844/1‑137 (MQ=255)
                                                                                          gACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGt                                         <  2:14383563/137‑1 (MQ=255)
                                                                                           aCCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTa                                        >  1:10066838/1‑137 (MQ=255)
                                                                                             ccGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCGCCAACCAGTACg                                      <  1:11950651/137‑1 (MQ=255)
                                                                                               gTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGc                                    <  1:1328000/137‑1 (MQ=255)
                                                                                               gTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGc                                    <  1:10999596/137‑1 (MQ=255)
                                                                                                   gCGTGTCGGCTTCGAAGTCCGTACCGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGACCATCGGTCTGATCCACTCACTGGCGGCCTATGCCCGCACCAACCAGTACGGCttac                                >  2:12540243/1‑135 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                >  2:11254944/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                <  1:4045139/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                <  1:3537395/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                >  1:17319327/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                >  2:4921847/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                <  1:1696823/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                >  2:16860729/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                <  2:11582997/137‑1 (MQ=255)
                                                                                                   gCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTcc                                >  2:12789410/1‑137 (MQ=255)
                                                                                                   gCGTGCCGGCTTAGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAAACAGTACGGCTTcc                                >  2:9750101/1‑137 (MQ=255)
                                                                                                    cGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCt                               <  2:2909486/137‑1 (MQ=255)
                                                                                                    cGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCt                               <  1:5578358/137‑1 (MQ=255)
                                                                                                    cGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCt                               >  1:6454216/1‑137 (MQ=255)
                                                                                                    cGTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCt                               >  2:10949969/1‑137 (MQ=255)
                                                                                                     gTGCCGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTg                              <  1:16667944/137‑1 (MQ=255)
                                                                                                         cGGCTTCGAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGaaa                          >  1:4820334/1‑137 (MQ=255)
                                                                                                                gAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTa                   <  2:10611757/137‑1 (MQ=255)
                                                                                                                gAAGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTa                   <  2:13506158/137‑1 (MQ=255)
                                                                                                                 aaGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAc                  <  1:4593563/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  2:9188047/137‑1 (MQ=255)
                                                                                                                  aGTCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCACACCAACCAGTACGGCTTCCTGGAAAGCCCGTAcc                 <  2:4532152/137‑1 (MQ=255)
                                                                                                                    tCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACcgc               >  1:17056318/1‑137 (MQ=255)
                                                                                                                    tCCGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACcgc               <  2:2008296/137‑1 (MQ=255)
                                                                                                                     ccGTGACGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACcgcg              <  1:6855194/137‑1 (MQ=255)
                                                                                                                         gACGTACACCCGCCCCACCATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCgtggt          >  2:3257645/1‑137 (MQ=255)
                                                                                                                           cGTACACCCGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTga        <  1:14981694/137‑1 (MQ=255)
                                                                                                                                 cccGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTgaaggaag  >  2:144997/1‑137 (MQ=255)
                                                                                                                                 cccGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTgaaggaag  >  1:5873119/1‑137 (MQ=255)
                                                                                                                                 cccGCCCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTgaaggaag  >  1:4374277/1‑137 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CAGCCAGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAG  >  AE015451_phaM1/539161‑539426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑