BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	1,070,248	+GA	intergenic (+48/+94)	aroP‑I CDS (aromatic amino acid transport protein) → / ← PP_0928 CDS (K+‑dependent Na+_Ca+ exchanger related‑protein)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,070,246	1	.	G	97.4%	2349.6 / 50.9	625	intergenic (+46/+96)	aroP‑I CDS (aromatic amino acid transport protein)/PP_0928 CDS (K+‑dependent Na+_Ca+ exchanger related‑protein)	–/–
Reads supporting (aligned to +/- strand): ref base . (8/8); new base G (290/314); total (299/326)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	1,070,246	2	.	A	97.4%	2208.1 / 45.8	625	intergenic (+46/+96)	aroP‑I CDS (aromatic amino acid transport protein)/PP_0928 CDS (K+‑dependent Na+_Ca+ exchanger related‑protein)	–/–
Reads supporting (aligned to +/- strand): ref base . (8/8); new base A (290/318); total (299/326)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 631 total aligned reads displayed.

ATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGG  >  AE015451_phaM1/1070111‑1070373

||

aTTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtgt‑‑g                                                                                                                                    >  2:9421281/1‑137 (MQ=255)

  tCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑ag                                                                                                                                <  1:16604811/137‑2 (MQ=255)

                 gCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCcgcgcg                                                                                                                 <  1:8491124/137‑1 (MQ=255)

                   tGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCgcggg                                                                                                               >  1:122046/1‑137 (MQ=255)

                     aTCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCgcgggcg                                                                                                             >  2:13198783/1‑137 (MQ=255)

                     aTCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGAGATGATCGGGTGTGTTCGTAAGGGGTATGCCGTTAGGCTTTCGGCGCCTGT‑‑G‑‑AGGTCGAGCGCCGCgcgggag                                                                                                             >  2:3412248/1‑135 (MQ=255)

                      tCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCgcgggcgg                                                                                                            <  1:9058427/137‑1 (MQ=255)

                        ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGgcggcg                                                                                                          >  1:13968734/1‑137 (MQ=255)

                        ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGgcggcg                                                                                                          >  2:8378054/1‑137 (MQ=255)

                         cGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCNCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGgcgc                                                                                                         >  1:1713582/1‑137 (MQ=255)

                          ggCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCt                                                                                                        >  1:4788144/1‑137 (MQ=255)

                             aTGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                     >  1:10877788/1‑137 (MQ=255)

                             aTGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGATTTGTTCTCACGGGGTCTGCCGTTAGGTTTTCAGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCCCGa                                                                                                     >  2:16510674/1‑137 (MQ=37)

                               ggATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                   <  2:8170834/137‑1 (MQ=255)

                               ggATCGCTGTGCTGTGGGCGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                   <  2:16255551/137‑1 (MQ=255)

                               ggATCGCGGTGCTGTGGGGGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                   <  2:7279603/137‑1 (MQ=38)

                                   cGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTcac                                                                                               <  1:2361560/137‑1 (MQ=255)

                                   cGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTcac                                                                                               <  1:15513802/137‑1 (MQ=255)

                                       gtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGc                                                                                           >  1:14831317/1‑137 (MQ=255)

                                        tgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCg                                                                                          >  2:16972593/1‑137 (MQ=255)

                                        tgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCg                                                                                          >  2:3962077/1‑137 (MQ=255)

                                        tgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCg                                                                                          >  2:4408943/1‑137 (MQ=255)

                                         gctgtgGGTGGCTTTCAAGCTGAGGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCAATCTCACAGGCGa                                                                                         <  2:15160251/137‑1 (MQ=38)

                                         gctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTTTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGa                                                                                         <  1:16925917/137‑1 (MQ=255)

                                         gctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGa                                                                                         <  2:10536867/137‑1 (MQ=255)

                                           tgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATg                                                                                       >  2:13481248/1‑137 (MQ=255)

                                             tgggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGaa                                                                                     >  1:18100484/1‑137 (MQ=255)

                                               gttggCTTTCAAGCTGAATAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAATGGTTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCACGCGGGCGGCGCTCGATCTCACAGGCGATGAAAg                                                                                   <  2:11990694/135‑1 (MQ=37)

                                                gtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc                                                                                  >  2:4640709/1‑137 (MQ=255)

                                                gtgaCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGGTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc                                                                                  <  1:10478961/137‑1 (MQ=38)

                                                       tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTaaaag                                                                           >  2:9254465/1‑135 (MQ=255)

                                                       tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAgg                                                                           >  1:11289511/1‑137 (MQ=255)

                                                       tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAgg                                                                           >  2:11110693/1‑137 (MQ=255)

                                                       tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAgg                                                                           >  1:5878890/1‑137 (MQ=255)

                                                       tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAgg                                                                           >  1:10163525/1‑137 (MQ=255)

                                                       tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAgg                                                                           >  2:2999274/1‑137 (MQ=255)

                                                          aGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCAt                                                                        >  2:16044591/1‑137 (MQ=255)

                                                           gCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCAAGCGCCGCGCGGGCGGCGCTCGACCTCACAGGCGATGAAAGCCTAAAGGCATg                                                                       <  2:8546116/137‑1 (MQ=38)

                                                                 gaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTg                                                                 <  1:8280943/137‑1 (MQ=255)

                                                                  aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCCCAGGCGATGAAAGCCTAAAGGCATGCGTCTgg                                                                >  2:15331231/1‑137 (MQ=255)

                                                                  aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTgg                                                                >  2:5514912/1‑137 (MQ=255)

                                                                       tCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                           <  2:7524002/137‑1 (MQ=255)

                                                                       tCGCCAGGCCAAGGGCTGATCAGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCAGCGCAGGCGGCGCTCGATCTCAAAGGCAATAAAAGCATAAAGGCATGCGTCTGGCGGcc                                                           >  2:17629563/1‑137 (MQ=37)

                                                                         gCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                         >  1:7015953/1‑137 (MQ=255)

                                                                            aGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAc                                                      >  2:888716/1‑137 (MQ=255)

                                                                            aGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAc                                                      >  1:1594979/1‑137 (MQ=255)

                                                                             ggCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAcg                                                     <  1:4108047/137‑1 (MQ=255)

                                                                                      cTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCt                                            <  1:3503313/137‑1 (MQ=255)

                                                                                         aTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                         <  2:3563451/137‑1 (MQ=255)

                                                                                         aTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                         <  2:14396601/137‑1 (MQ=255)

                                                                                         aTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                         <  1:3888015/137‑1 (MQ=255)

                                                                                         aTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑CCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                         <  1:11114819/137‑1 (MQ=255)

                                                                                           cGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCAt                                       >  1:3231302/1‑137 (MQ=255)

                                                                                           cGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCATTCCAt                                       >  1:1764374/1‑137 (MQ=255)

                                                                                            gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATg                                      >  2:6766829/1‑137 (MQ=255)

                                                                                            gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATg                                      >  1:10536988/1‑137 (MQ=255)

                                                                                            gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATg                                      >  1:14435668/1‑137 (MQ=255)

                                                                                            gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATg                                      >  1:16115859/1‑137 (MQ=255)

                                                                                            gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATg                                      >  1:2820600/1‑137 (MQ=255)

                                                                                            gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAATGAAAGCCCAAAGGCATGCGTCTGGCAGCCCTGACGCGTTCCCCTTCCATg                                      >  1:11864323/1‑137 (MQ=37)

                                                                                            gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCAGCGAGGGTGGCGCTCGATCCCACAGGCGATGAAAGCATAAAGGAATGCGTATGGCGGCCCTGACGCGTTCCCCTTCCATg                                      >  1:18305990/1‑137 (MQ=255)

                                                                                                 tGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCt                                 >  2:4845342/1‑137 (MQ=255)

                                                                                                   ttCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCa                               <  1:6155456/137‑1 (MQ=255)

                                                                                                      gTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtg                            >  1:15794703/1‑137 (MQ=255)

                                                                                                        aaGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                          <  2:5940508/137‑1 (MQ=255)

                                                                                                             gTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTc                     <  1:5365810/137‑1 (MQ=255)

                                                                                                             gTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTc                     <  1:7413993/137‑1 (MQ=255)

                                                                                                             gTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTc                     <  2:11814802/137‑1 (MQ=255)

                                                                                                              tATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTcc                    <  2:2378761/137‑1 (MQ=255)

                                                                                                               aTGCCGTTAGGTTTTCGGCGCCTGGGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCg                   <  1:13293384/137‑1 (MQ=255)

                                                                                                                   cGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCACGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCaa               >  1:7860963/1‑137 (MQ=255)

                                                                                                                   cGTTAGGTCTTCGACGCCTGT‑‑GAAA‑‑TCGAACGCCGCGCGGGCGGCGCTCGATCTCACAGGCAATGAAAACCTCAAGGCATGCATCTGGCGGCCCTGACGCGTTCCACTTCCATACCCCTCACTGCTGCCTCCGcaca               >  2:9046751/1‑134 (MQ=25)

                                                                                                                    gTTAGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAg              >  1:16241244/1‑137 (MQ=255)

                                                                                                                       aGGTTTTCTGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCTCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac           <  2:2071726/137‑1 (MQ=38)

                                                                                                                       aGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac           <  1:17024318/137‑1 (MQ=255)

                                                                                                                       aGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac           <  1:12854391/137‑1 (MQ=255)

                                                                                                                       aGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac           <  2:1164517/137‑1 (MQ=255)

                                                                                                                       aGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac           <  2:17883621/137‑1 (MQ=255)

                                                                                                                       aGGTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac           <  2:9741061/137‑1 (MQ=255)

                                                                                                                       aGGTCTTCTGCGCCTGT‑‑GATA‑‑TCGAGCGCCGCGCGGGCGTCACTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCTTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac           <  2:14810668/137‑1 (MQ=38)

                                                                                                                        ggTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacg          >  1:9991564/1‑137 (MQ=255)

                                                                                                                        ggTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacg          >  1:9595780/1‑137 (MQ=255)

                                                                                                                        ggTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGAGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacg          >  1:1751617/1‑137 (MQ=255)

                                                                                                                         gTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc         <  1:1248186/137‑1 (MQ=255)

                                                                                                                         gTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc         <  2:8003008/137‑1 (MQ=255)

                                                                                                                         gTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc         >  1:5576551/1‑137 (MQ=255)

                                                                                                                         gTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc         >  2:647007/1‑137 (MQ=255)

                                                                                                                         gTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc         <  2:18401499/137‑1 (MQ=255)

                                                                                                                         gTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc         >  1:14147580/1‑137 (MQ=255)

                                                                                                                         gTTTTCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc         <  2:13906276/137‑1 (MQ=255)

                                                                                                                          ttttCGGCGCCTGTTAG‑‑A‑‑TCGATCGCCGCGCGTGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgca        <  1:13811244/137‑1 (MQ=39)

                                                                                                                          ttttCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgca        <  2:12772922/137‑1 (MQ=255)

                                                                                                                          ttttCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTACCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgca        <  2:15599534/137‑1 (MQ=38)

                                                                                                                          tgttCGGCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgca        <  1:18614074/135‑1 (MQ=255)

                                                                                                                               ggCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGTTGCCTCCGCCAAGCACGCACAgtg   <  1:16508155/137‑1 (MQ=38)

                                                                                                                               ggCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg   >  1:645951/1‑137 (MQ=255)

                                                                                                                               ggCGCCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTAACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg   <  2:12372629/137‑1 (MQ=255)

                                                                                                                                gcgcCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCCCAGGCGATGAAAGCCTAAAGGCCTGCGTCTGGCGGCCCTGAAGCGTTCCCCCTCCATGCCCCTCCATGCTGCCTCCGCCAAGCACGCACAgtgg  >  1:7784119/1‑137 (MQ=37)

                                                                                                                                gcgcCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtgg  >  2:1727626/1‑137 (MQ=255)

                                                                                                                                gcgcCTGTGAG‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtgg  >  1:4422520/1‑137 (MQ=255)

||

ATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑A‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGG  >  AE015451_phaM1/1070111‑1070373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`