BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,344,308	0	G	A	30.7%	124.0 / 17.1	111	intergenic (+39/‑117)	PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base G (50/20); new base A (30/1); total (90/21)
Fisher's exact test for biased strand distribution p-value = 2.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 147 total aligned reads displayed.

CGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344172‑4344443

cGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccg                                                                                                                                           <  2:16521131/137‑1 (MQ=255)

cGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccg                                                                                                                                           <  2:16521099/137‑1 (MQ=255)

cGGGCGACAGCGTGAAGGTGCGTTGGGCAGGGGCAGACGCTTGCATGTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccg                                                                                                                                           <  1:7030960/137‑1 (MQ=255)

 gggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgc                                                                                                                                          <  2:6607477/137‑1 (MQ=255)

  ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcc                                                                                                                                         <  2:3338464/137‑1 (MQ=255)

  ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcc                                                                                                                                         <  2:3338501/137‑1 (MQ=255)

  ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGAaccacc                                                                                                                                         >  2:8107665/1‑137 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCCGACGCTTGCATTTCTACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGTGTCTTCCATAGCTAGCAccaccc                                                                                                                                        >  2:7378107/1‑137 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCAACCAAGCAccaccc                                                                                                                                        >  2:7378133/1‑137 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:18574137/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:11457956/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:7482788/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  1:18616615/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:7159816/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:54678/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  1:8468066/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:3897040/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  1:1234411/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  1:12123621/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  1:11496029/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCACTTATACCGAATGGGCCGAGAGCGACCTAAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACCTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:11823655/137‑1 (MQ=255)

   gCGACAGCGTGAAGGTGCGGTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgccc                                                                                                                                        <  2:3935622/137‑1 (MQ=255)

    cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                       <  2:1078491/137‑1 (MQ=255)

     gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccgc                                                                                                                                      <  2:2898148/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGTGTAGTGGCGGACGCTTGCATTTAGACCGAATGTGCCGAGAGCGATCCGAGCAAGTTGGCCGGGGCGTATTAACGCTCTTGTACGGTGCAGCGCCGGACAGATCGAGCACCGCCcgcgcg                                                                                                                                   <  1:18110189/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                   <  1:2157845/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                   <  1:15942921/137‑1 (MQ=255)

        aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAAATTGAGCACCGCCcgcgcg                                                                                                                                   >  2:14918511/1‑137 (MQ=255)

          cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                                 <  2:7927617/137‑1 (MQ=255)

          cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCTCCAGATCGAGCACCGCCCACGCggg                                                                                                                                 >  2:7604797/1‑136 (MQ=255)

          cGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGACCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGc                                                                                                                                 <  2:5433670/137‑1 (MQ=255)

           gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGca                                                                                                                                <  1:18551409/137‑1 (MQ=255)

             gAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCAAAGCAAACCCCGCCCACGCGGcaca                                                                                                                              >  2:8909817/1‑137 (MQ=255)

              aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                             <  1:11410417/137‑1 (MQ=255)

              aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                             <  1:5799408/137‑1 (MQ=255)

              aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                             <  2:4381309/137‑1 (MQ=255)

              aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                             <  1:3680605/137‑1 (MQ=255)

              aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                             <  1:17688376/137‑1 (MQ=255)

               aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCAATCCAGCCCCACCCCCCCCGcacccc                                                                                                                            >  1:17126011/1‑134 (MQ=255)

                 gTGCGTTGGGCAGGGGCAGACGCTGGCATTTATACCGAATGGGCCGAGAGCGACCTGAACAAGTCGGCCGGGGCGCATTAACGCTTTTGGATGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGAt                                                                                                                          <  2:9514935/137‑1 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGGATTTATACCGAATGGGCCGAGAGTGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCCGGTACGTTGCAGCGCCTTCCAAACCAAGCACCGCCCCCAGGGCACACGATC‑Tggga                                                                                                                   >  2:1218977/1‑135 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGTGCCTGCCCGACCGAGTAACGCCCGCGCGGGACACGATC‑TGGCa                                                                                                                   >  2:17479671/1‑137 (MQ=255)

                       tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCCGCCCGACCGCACAACGCCCACCCAGAACAAAATC‑TGGCa                                                                                                                   >  1:14153514/1‑137 (MQ=255)

                               ggCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAGACCGAGCACCGCCCGCGCGGCACACGATC‑TGGCAAGCGCTGc                                                                                                           >  2:18323848/1‑137 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGTCTAATCTAGCCCCACCCGCGCAGCACAAAATC‑CGGGAGGCGTTGCAc                                                                                                         >  1:18148178/1‑137 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAAAGCGAGCACCACCCCCGCAGAACACAAGC‑TGGCAGGCGATGcca                                                                                                         >  2:287936/1‑135 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGATGTCAAATCGAGCAACACCCACGCGGGCCAAGATC‑TGGCCAGCGCTTCAc                                                                                                         >  1:10137150/1‑137 (MQ=255)

                                 cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCTCCCAACCTAACACCACCCCCGCGTCCCCCAATC‑TTGCCGGCGCTTCAc                                                                                                         >  1:9282261/1‑137 (MQ=255)

                                  aGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCACCCGCGCGGCACACGATC‑TGGCAGGGGCTGGACt                                                                                                        >  2:5074756/1‑137 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCGATCAAGCCCCACCCCCGCCGAAACAAAGC‑TGGCCGGAGCTGCACT‑actt                                                                                                   >  1:1520274/1‑134 (MQ=255)

                                      gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAAATCGAGAACCACCCCCGCGGCACACAAGC‑CGGCAGGAGCTGCaacactg                                                                                                    >  1:1512353/1‑131 (MQ=255)

                                           cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCGGCGGCTGCCCGATCTAGCCCCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCAAT‑ACTGGAggg                                                                                              >  1:12509419/1‑136 (MQ=255)

                                             tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGATGTCAGATCGAGCACCGCCCCCGCGGTACACAATC‑TGGCCAGAGCTGCCCC‑ATTGGGGGAGt                                                                                            >  1:13942606/1‑137 (MQ=255)

                                             tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCCAACACCGCACGCGCGGCACACGATC‑TGGCAGGCGCCGCACC‑CTTGGAGGCGt                                                                                            >  2:10248855/1‑137 (MQ=255)

                                             tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAGATCCAACACCGCCCGCACGGCACACGACC‑TGGCCGGAGATTCACC‑ATTTGAGGCGt                                                                                            >  2:15851627/1‑137 (MQ=255)

                                                   ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGTGCCTGCCCGACCGAGCACCACCCACGCGGAACCCAATC‑TGGCAAGAGATGCACC‑CCTGGAGGCGTACGccc                                                                                      >  2:13942832/1‑137 (MQ=255)

                                                   ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCAATCGAGCACCGCCCCCGCCGCACACGAGC‑TGGCAGGCGCTGCACC‑CTTTGAAGCTTACGacc                                                                                      >  2:6591042/1‑137 (MQ=255)

                                                   ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATTGAGCACCGCCCGCGCTGGAAACAATC‑TGGGAGGGGCTGAACT‑CTTGGGGGTGTACGtcc                                                                                      >  2:9200471/1‑137 (MQ=255)

                                                   ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAACAACCACGCGGCACACGATC‑TTGCAAGCGCTGTCCT‑ATTTGGAGCGTCCGccc                                                                                      >  2:15859501/1‑137 (MQ=255)

                                                        tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGGGCACCACCCGCGCGGTACACAATC‑TGGTAGGCGCTGCCCG‑ATTGGGGGCGTACGCCCCATcc                                                                                 >  2:14076749/1‑136 (MQ=255)

                                                        tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCGAACACCACCCGCGCGGCACACAACC‑TGGCAAGCGCCGCACC‑ATTTGAGGCGTACGCCACATca                                                                                 >  2:2512072/1‑137 (MQ=255)

                                                        tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAGATCGAGCACCGCCCACGCGGCACAAAAGC‑TGGAAGGCGCTGTAAC‑ATTGGAGGCGTACGCCCCGTca                                                                                 >  2:17672299/1‑137 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTCCCAGATTTAGTACCCTCCGTGCGGCACAACAGC‑TTGCAAGGTCTGCCCT‑ACTTGAGACGTACGacccattaa                                                                                >  2:10210761/1‑132 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAGACCGAAAACCACCCACGCAGCACACAATC‑CGGCAAGTGATGGACC‑ATTTGAGGAGTCCACCCCGTcaa                                                                                >  1:18258846/1‑136 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAACACCCCCCCCACGGAACAAAATC‑TGGCAGGCGCTGGACT‑CTTGGAGGCGTACGCCCCCTcaa                                                                                >  2:7565065/1‑136 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCGAGCACCCCCCGCACGGGACACGAGCTTGGCA‑GAGCTTTACT‑ACTTGGGGCGTAccccccaccac                                                                                >  2:9890490/1‑132 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACTGCCCGATCGAGAACCCCCCCCACGGCACACGACC‑TGGAAAGCGCTGCACT‑CTTTGAGGCGTACAccccaccac                                                                                >  2:12058524/1‑132 (MQ=255)

                                                         gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCGCCAAATCAAGCACCACCCACGCGGAACACAAAC‑CAGAAGGAGCTGCCCT‑ACTTGGGGCGTACGccccagcac                                                                                >  2:7565064/1‑132 (MQ=255)

                                                          ggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                       >  1:4963308/1‑83 (MQ=255)

                                                          ggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                       >  1:4963929/1‑83 (MQ=255)

                                                          ggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgcccg                                                                                                                                       <  2:4963929/83‑1 (MQ=255)

                                                              gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAGATCGAGCACCGCCCGCGCGGCACACGAGC‑TGGCAGGCGCTGCACC‑ATTGGAGGCATAAGCCCCGTCCCACTac                                                                           >  2:17282597/1‑136 (MQ=255)

                                                              gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCCGCCAAATCGAAAACCACCCGCACAGCACACAATC‑TTGCAAGCGCTGCACC‑ACTGGAGGCGTCCACCCCCTCACACTat                                                                           >  2:17282561/1‑137 (MQ=255)

                                                                 agCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAAATCGAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCAAT‑ATTGGAGGAGTACGCCCCATCACATTatcac                                                                        >  2:12983606/1‑136 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCCAATCGAGCACCACCCACGCGGCACAAAAGC‑TGGCAAGAAATGCACT‑ACTGGAAGCGTACCCCCCACCACACTCTCCTCCCGCCa                                                                 >  2:15449963/1‑137 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCCAATCGAACACCCCCCCCCCGGACCAAAACC‑TGGCAGGCGCTGCACT‑ACCTGAGGGGTGCACCCCATCACACTCTCCTCAccccc                                                                 >  2:412834/1‑136 (MQ=255)

                                                                        tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCATACCCCACGTGGACCACGGGC‑TTGGAGGCGCTGTAAT‑CTTGGGGGTGTACGTCCCGTTGAATTTTCATCCCGCCa                                                                 >  2:18265488/1‑137 (MQ=255)

                                                                             aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCCGATTGAGCACCCCCCACGCGGCACACGAGC‑TTGCCGGCGCCTCACC‑CTTTGGGGCGTCCACCCCCTCCCACTCTCCTCCCACCACTGTc                                                            >  2:8160086/1‑137 (MQ=255)

                                                                             aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATC‑TTGAAAGCGCTGCACT‑ATTGGAGGAGTACACCCCCTCACATTATCATCACGCCACTATc                                                            >  2:17588517/1‑137 (MQ=255)

                                                                             aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGACCGCCAAACCCAACACCACCCGCACGGTACACAATC‑CTGTAAGCGCTGCCCC‑ATTGGAGGAATACGCCCCATCCCACTATCCCCACACCACtctc                                                            >  2:9875665/1‑137 (MQ=255)

                                                                                  ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGTGCGGCGCACGATC‑TTGCAGGTGCCGCAAT‑CTTGGAGGCGTCCACTCCGTCCCATTGTCTTCACGCCCCTGTCGTCAt                                                       >  2:15296179/1‑137 (MQ=255)

                                                                                   gCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCTGCCAAACCGAGCACCACCCGCGCAGCACACAATC‑TTGTAGGAGCTTCCCTCCTTGGA‑GCGTCCGCCCCATCACCCTATCATCCCGCCACTGTTGTAATc                                                      >  2:11871729/1‑137 (MQ=255)

                                                                                       ggtcgcATGAACGCTCTGGTATGTGGAAGCGCTTGCCAGATCGAGCACCACCCGCGCGGCACACGACC‑GGGAAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGcc                                                  <  2:13839944/134‑1 (MQ=255)

                                                                                       gggCGCATTAACGCTCTTGTACGTTGCAGCGGCTGCCAAACCGAGCACCACCCACGCGGCACACGATC‑TGGCAGGGGCTGCACT‑ATTGGAGGAGTACGCCCCGTCCCCTTCTCATTCCGCCACCTTCGTAACCTTcc                                                  >  2:9292237/1‑137 (MQ=255)

                                                                                       gggCGCATTAACGCTCTTGTACGTTGCAGCGCATTCCCGATCCAGCCCCACCCGCATGGGACCAAATC‑TGGCAAGAGCTTCACC‑CTTTGAGGCGTAAACCCCGCCACATTATCACCACAACACTTGCGTCATCTGcc                                                  >  2:9441815/1‑137 (MQ=255)

                                                                                        ggCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACAAGATC‑TGGTAGGTGCTGTCCT‑ATTGGAGGCGTACACCCCGTCACATTCTCCTCACGCCCCTGTCGTAATCTGCCg                                                 >  2:13847942/1‑137 (MQ=255)

                                                                                                                  aGCGGCTTCCAAATCGAGCACCGCCCGCGCGGCACACAGTC‑TGGCAGGGGCTGCAAT‑ATTGGAGGAGTACGCCCCATCACATTATCATCACACCACTTTCGTAATCTGCCGGACAACTCCATGCCACAGGTCACCCg                       >  2:13408813/1‑137 (MQ=255)

                                                                                                                  aGCGCCTGCCAGATCGAGCACCGCCCGCGGGGCACACAACC‑TGGCCGGCGCTGCACC‑CTTGGAGGCGTACGCCCCACCACATTCTCCTCCCCCCACTGTCATCATCTTCCGGCCAACTCTCTTCCACAGGTTAccct                       >  2:8953892/1‑136 (MQ=255)

                                                                                                                    cgcCTGCCAGATCGAGCACCGCCCGCGCGGCACACAATC‑TGGCAGGCGCTGCACT‑CCTGGAGGCGTACGCCCCCTCACAGTCTCATCCCGCCAATGTCGTAACCTGCCAGCCAAACCTCTGCCACAGGTCACCCGGc                     >  2:13734343/1‑137 (MQ=255)

                                                                                                                    cgcCTGCCAGAGCTAGCACCACCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ACTGGAAGAATCCGCCCCCTCACATTATCATCACGCCACTGTTGTAATCTGCCGGACAACCCTCTGCCACAAGTCACCCAGc                     >  2:7008405/1‑137 (MQ=255)

                                                                                                                       cTGCCAGACCGAGCACCACCCACGCGGCACACAATC‑TGGACGGCGGTTCACC‑ACTTGAGGCGTACGCCCCGTCACACTACCCTCCCCCCACCCTCGTAATCTTCCGGACAACCCTCTGCCAAAAGTTCCCCAGCATg                  >  2:18429577/1‑137 (MQ=255)

                                                                                                                            cgATCGAGCACCGCCCGCGTGGTGCACGGTT‑TTGTAGGTGTTGTACT‑GTTGGGGGTGGACGCCCCTTTACATTGTCCTTACGCTACTGTTTTGGTCTGTCTGCTAACTCTGTGCCACAGGTCACCCGGCGTGCttcg             >  2:18087007/2‑134 (MQ=255)

                                                                                                                                 gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGCGGCGTACGCCCCGTCACAGTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  2:111907/1‑137 (MQ=255)

                                                                                                                                 gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCCCGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTgt        >  1:16432598/1‑136 (MQ=255)

                                                                                                                                 gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTCTGCCACCGGTCACCCGGCATGCTCCGCCTGc        >  1:6144185/1‑137 (MQ=255)

                                                                                                                                 gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  2:13815938/1‑137 (MQ=255)

                                                                                                                                 gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCAAGCCACTGTCGTAATCTGCCGGCAAACCCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  2:13815977/1‑137 (MQ=255)

                                                                                                                                 gAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCATCACATTATCATCAAGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc        >  1:9308009/1‑137 (MQ=255)

                                                                                                                                   gCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCCCGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCCGCtt      >  2:12899202/1‑137 (MQ=255)

                                                                                                                                       cgcccgcGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt  >  1:14282951/1‑137 (MQ=255)

CGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATC‑TGGCAGGCGCTGCACT‑ATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCT  >  AE015451_phaM1/4344172‑4344443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`