Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,889,6230AG33.3% 183.8 / 26.9 130intergenic (+69/‑84)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
Reads supporting (aligned to +/- strand):  ref base A (55/31);  new base G (0/43);  total (55/75)
Fisher's exact test for biased strand distribution p-value = 2.06e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

AGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCT  >  AE015451_phaM1/4889496‑4889757
                                                                                                                                 |                                                                                                                                        
aGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGc                                                                                                                                <  1:15941117/137‑1 (MQ=255)
      tGACCACCACCGAAGCGGGCCTGGGCGGGCTGAAAACCC‑ACAACGCAGTCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑cgcg                                                                                                                         <  2:15668176/137‑1 (MQ=255)
       gACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGTTCTCCCGCGAACACCGGGAAAGCCGGTTCCC‑CCCACCGAGTT‑TTCTCTTT‑CGCgg                                                                                                                        >  2:17943834/1‑137 (MQ=255)
         ccaccacgGAAGCGGGCTTGGGTTGGCGGAAAACCC‑ACACAACAATCTGAGGAACAATTTGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAATCCGGTGCCA‑TCCACCGGGTT‑GTCTGTTT‑CGCGGGc                                                                                                                      <  2:11803115/137‑1 (MQ=25)
         ccaccaccGAAGGGGGCTTGGGTGGGCGAAAAACCC‑ACAACGCAATCGGAGAAACACCTTGGAACCTTGGGGGAGCGGGTTCGCCCGAGAACACCGGAAAAGCTGGTGCCA‑TCCACCGGTTT‑GTTTGTTT‑CGTGGGc                                                                                                                      <  2:2167445/137‑1 (MQ=255)
         ccaccaccGAAGCGGGCCTGGGTTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGc                                                                                                                      <  2:16630065/137‑1 (MQ=255)
         caaccaccGAAGAGGACTTGGGTTGGCGGAAAACCA‑AAAACACAATCTGAGGAACAACGCGGGACCTTGTGGGAGCGGGCCCTCCCCCAAACACCGTCAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTTCTT‑CGCggtc                                                                                                                      <  2:15895277/135‑3 (MQ=255)
                     tgggTCTGGGCTGGCGGAAAACCC‑ACAATGCAATCTGAGGAACACCTCGGGACCCTGTGGGAGCGGGCTCGCCAGCGAACACCGGCAAAGCTGGTGCCA‑TCAACCGGGTT‑GTCTGCTT‑CGCGGGCGACCCCGCTccc                                                                                                          <  2:4472862/136‑1 (MQ=37)
                     cGGGCTGGGGCTGGCTGAAAACCC‑ACAACGCAATCTGGGGAACAACTTGGGACCCTGGGGGAGCGGGCTCGCCAGCGAACACCGGAAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑TGCGGGCGATCCCGCTccc                                                                                                          <  2:12737428/137‑1 (MQ=255)
                     agggCCTGGGCTGGCTAAAAACCC‑ACAACGCAACCGGAGGAACAACTCGGGACCCTTTGGGAGCGGGCTCGCCAGCGAACACTGGCAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCGGCTT‑CGCGGGCGAGCCCGCTccc                                                                                                          <  2:12737391/136‑1 (MQ=38)
                      gggCCTGGTCTGGCGAAAAACCC‑ACAACGCAATCTGAGGAACACCTCGGGACCTTTTGGGGCCGGTCTCCCCCGCGAACACCGGCAAAGCTGTGGCCC‑TCCACCGGGTT‑GTTTGCTT‑CGGGGGCGAGCCCGCTCCca                                                                                                         <  2:13752434/137‑1 (MQ=25)
                          cTGGGCTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGGTTGGCCTCTAACCCCGGCCCAACCGGTTCCC‑CCCCCCCAGTT‑TTCTTCTT‑CTTTGGTGAGTCCCCTCCcccaag                                                                                                     >  1:16277619/1‑135 (MQ=255)
                          cTGGGCTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACCCCAGCAAAGACCGTGCCC‑CCCACCAAGGT‑GTCTGCTT‑TGCGGGGGAGGCCGATCCcaacag                                                                                                     >  2:4249968/1‑133 (MQ=25)
                          cTGGGCTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGATTGCCCACGAACACCAGAAAAAACGGCGTCA‑TCCACCCAGGT‑GTTTTCTT‑CTCTGGGGAGCCCTCCCCcccaag                                                                                                     >  2:1848996/1‑135 (MQ=255)
                                 ggctgAAAACCC‑ACAACGCAGTCTGAGAAACAACTCGGGACCCTGGGGGAGCGGGCTCGCCCGCGAACACCGGCAAATCCGGTGCCA‑CCCACCGGGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCaa                                                                                              <  2:13647384/137‑1 (MQ=25)
                                 ggcgaaaacCCC‑ACAACGCAACTGGAGAAACAACTCGGGACTTTGTGGGGGTGGGTTTGCCCGCAAACACCGGCAAGTCTGGTGCCA‑TCCATTGGTTT‑TTCTGTTT‑TGTGGGCGACCCCGCTCCCACAGGGGGCCaa                                                                                              <  2:10458619/132‑1 (MQ=255)
                                 gactgAAAACCC‑ACAACGCACTCTGGGAAACACCTTGGGACCCTGTGGGGTCGGGTTCGCCCGAGAACACCGACAAAGCCGGTGCCC‑TCCACCTCTTT‑GCCTGCTT‑CGCGGTCGATCCCGCTCCCACAGGGGGCCaa                                                                                              <  2:16958252/135‑1 (MQ=19)
                                  ggttaaaaCCCAACAACGCACTCTGGGGAACACCTCGGGACCTTTTGGGAGTGGTCTCTCCCGCAAACACCGGCAACACTGTTCCCA‑CCCCCCGGGTT‑TTCTCCTC‑CGTGGCCGCCCCCGCTCCCACAGGGGGCCaa                                                                                              <  2:5092780/133‑1 (MQ=255)
                                     gAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGGGCGCCCACGAACACCAGCAAAAACGGTGTCC‑CCCACCAAAGT‑TTCTTCTT‑CCCAGGAAAAACCCCTCCCACAAAGGGCCcaaaat                                                                                          >  2:3179742/1‑134 (MQ=255)
                                      aaacCCC‑AAACCACACTCGGAGAAACACTTTGGAACCTTGGGGGGGGGGGCTCTCCCAAGAAAACAGGAAAAGCGGGTTCCA‑TCCCCTGGTTT‑GTTTGTTT‑CGTGTGCAACCCCGCTCCCACAGGGGGCCAAAgctt                                                                                         <  2:8486154/137‑1 (MQ=255)
                                               caACGCAATCGGAGGAACACCTTGGGACCTTGTGGGGGTGGGCTCGCCCGAGAAAACGGGAAAAGTTGGTGCCA‑TCCACGGATTT‑TTCTGTTT‑CGCGGACGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                 <  2:16553586/137‑1 (MQ=255)
                                               caACACACTCTGAAGAACACCTGGGGCCCTTGTGGGAGCGGGTTTGCCCGCGAACACCGACAACGCCGGTGCCC‑TCCACCGGTTT‑GTCTTCTT‑CGGGGACAATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                 <  2:10547311/137‑1 (MQ=19)
                                               caACACAATCTGGGAAACAACTTGGACCCTTGGGGGAGCGGGCTCGCCCGAGAACACCGGC‑AAGTCGGTTTCACTCCACCGGTTT‑CTTTTTTT‑CGCGGCCGGTCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                 <  2:2249786/137‑1 (MQ=255)
                                               aagcacAATTTGAGGAACACCTCGGGACCCTTTGGGGGCGGTTTCTCCCGAGAACACCGGCAAAGCCGGTGCCA‑TCCACTGGGTT‑TTCTGTTT‑CGTGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                 <  2:4109790/132‑1 (MQ=25)
                                               aaaCGCAATCGGAGAAACAACTCGGGACCCTGTGGGAGCGTGCTCGCCCGCAAACACCGACAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGTGGACGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                 <  2:15383593/136‑1 (MQ=37)
                                                 ccGCACTCGGAGAAACACTTTGGGACCTTGGGGGAGCGGGTTCCCCCGAAAAAACGGAAAAAGCTGTTGCCG‑TCCACGGGGTT‑TTTTTTTT‑CGTGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGata                                                                               <  2:15594386/136‑1 (MQ=255)
                                                 aCGAAATCGGAGAAAAAACTTGGGACCTTGGGGGAGTGGGCTTTCCGGGAAAAACTGGAAAGGCTGGTGCCA‑TCCACTGGGTG‑GTTTTTTT‑CGCGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGata                                                                               <  2:18033764/137‑1 (MQ=255)
                                                         tGAGGAACACCTTGGGACCTTGTGGGAGCTTTCTTTCCCGAGAACACCGAAAAAGCTGGTGCCA‑TCCATTGGTTT‑GTTTGTTT‑TGGGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAtt                                                                       <  2:1932537/137‑1 (MQ=255)
                                                         ggagaAACACCTTGGGACCTTGTGGGGGGGGTTTTCCCCGAAAACACTGAAAAAGCTGTTCCCA‑‑CCCCCGGGTTGGTCTGCTT‑CGTGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAtt                                                                       <  2:5297877/136‑1 (MQ=255)
                                                           agaaacaacTGGGGACCTTGGGGGGGAGGGCTCGCCCGCGAACACCGGCAAAGCTGGGGCCA‑TCCACCGATTT‑TTCTGCTT‑CTCGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTAc                                                                     <  2:14195832/134‑1 (MQ=25)
                                                             aaacaccTCGGAACCCTGTGGGAGAGGGCTCGCCCGAGAACACCGGAAAAGCAGGTGCCA‑TCCACCGGGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACaa                                                                   <  2:5883916/136‑1 (MQ=255)
                                                                   cttggaaCCCTGTGGGAGTGGGCTCGCCCGCAAACACCGGAAAAGCTGGTGCCA‑TCCACTGGGTT‑GTCTGCTT‑CGCGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGt                                                             <  2:7269366/131‑1 (MQ=255)
                                                                           ccTGTGGGATCGGGCTCGCCCGCGAACACCGGCAAAACCGGTCCCA‑TCCACGGAGTT‑GTCTGCTT‑CGTGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAATAGGTAATTtctc                                                     <  2:4629733/137‑1 (MQ=37)
                                                                                               cgaaaacaacGGAAAAGCGGGTACCA‑TCCACCGGGTG‑GTTGTCTT‑CGAGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCAt                                 <  1:8831751/133‑1 (MQ=255)
                                                                                               cgaaaaCACCGACAAAGCTTGTGCCA‑CCCACCGGTTT‑GTTTTCTT‑CGTGCGCGCGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCAt                                 <  1:14411396/133‑1 (MQ=255)
                                                                                                 aaaaCACTGACAAA‑CCCGTGCCC‑CCCCCCCAGTT‑TTCTTCTTCCGAGGCCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCg                               <  1:18107056/135‑1 (MQ=255)
                                                                                                   aaCACCGGCAACGTGGGTGCCA‑TCCACTGATTT‑TTCTGCTT‑CGCGGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGc                             <  1:18471967/137‑1 (MQ=255)
                                                                                                    acacCGGAAAATCTGGGACCA‑TCCACCGATTT‑GTCGTCTT‑CGAGGGCGGCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCa                            <  2:12543916/137‑1 (MQ=255)
                                                                                                     cacagacAAATCCGTTCCCC‑TCCACCGATTT‑TTCTGCTT‑CGGGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAc                           <  1:11970543/131‑1 (MQ=255)
                                                                                                         ggCAAAGCAGGTACCA‑TCCACCGAGTC‑TTTTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                       <  2:6918015/137‑1 (MQ=255)
                                                                                                         ggCAAACCCGGTACCA‑CCCACCGAGTG‑GTCTGCTC‑CGCGGGCAAACCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                       <  2:6889154/137‑1 (MQ=255)
                                                                                                         gaaaaaGCTGTTGCCA‑CCCACGGGGTT‑TTCTGCTT‑CGCGGACACCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                       <  2:3662859/134‑1 (MQ=255)
                                                                                                          aaaaaGCCGGTACCA‑TCCCTCGGGTT‑TTCGGCGT‑TGTGGGAGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGca                      <  1:16861882/135‑1 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCCAAGCTTGCTTGGGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAGTCGGTTTCATCGGCACCGGcat                     >  1:12713323/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAACTCGGTTTCATCGGCACCGGcat                     >  1:15975228/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  2:5984609/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  2:6601761/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  1:13189550/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  2:11755638/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  2:137110/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  2:11239026/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  2:11239000/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  2:5984608/1‑137 (MQ=255)
                                                                                                           cAAAGCCGGTGCCA‑TCCAACGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                     >  1:1260761/1‑137 (MQ=255)
                                                                                                           cAAAGCCAGTGCCA‑CCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATCACAAAGAGGTCGTTTCTCATGGCTAAAATCGGTCTCATCGTCACCGGcat                     >  1:3533800/1‑137 (MQ=255)
                                                                                                             aaGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatca                   >  2:17808188/1‑137 (MQ=255)
                                                                                                             aaGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatca                   >  1:12289648/1‑137 (MQ=255)
                                                                                                             aaGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatca                   >  1:12652335/1‑137 (MQ=255)
                                                                                                               tcgggTGCCA‑CCCCCCGATTT‑GTCTGCTT‑CGAGGACGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  2:1689230/134‑1 (MQ=255)
                                                                                                               tccGGTACCA‑TCCACTGGGTT‑GTTTTCTC‑TGTGGTAGAGCCCGCTCCCACAGTGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  2:11741266/136‑1 (MQ=255)
                                                                                                               gtcGGGGCCA‑TCCATTGGTTT‑GTTTGTTT‑CGCGGATGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTAAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  1:13509374/135‑1 (MQ=255)
                                                                                                               gctggggCCA‑CCCACGGAGTT‑TTTTGTTT‑CGTGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  2:11741213/131‑1 (MQ=255)
                                                                                                               gctggTGCCA‑CCCACGGGGTT‑GTCTGCTT‑CGTGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  2:10975752/134‑1 (MQ=255)
                                                                                                               gcgggTTCCA‑CCCACGGGTTT‑GTTTGTTT‑TGTGGGCCATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  1:6619129/134‑1 (MQ=255)
                                                                                                               gcgggTGCCA‑CCCACTGGGTT‑GTTTTTTT‑CGTGGGAGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  2:2862518/134‑1 (MQ=255)
                                                                                                               gcgggTGCCA‑CCCACCGGTTT‑GTTTTTTT‑CGGGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                 <  2:3933661/134‑1 (MQ=255)
                                                                                                                 tggTGCCA‑TCCACCGATTT‑TTTTGCTT‑CGCGGGCGGCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATggg               <  2:7569067/136‑1 (MQ=255)
                                                                                                                 cGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATggg               >  1:7314252/1‑137 (MQ=255)
                                                                                                                   gTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa             >  2:7835318/1‑137 (MQ=255)
                                                                                                                   gTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa             >  1:18518108/1‑137 (MQ=255)
                                                                                                                   gTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa             >  2:5358140/1‑137 (MQ=255)
                                                                                                                   gTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa             >  2:15579386/1‑137 (MQ=255)
                                                                                                                     ttccctCCACCGAGTT‑TTCTGCTC‑CGTGCGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAGTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCaa            <  2:13913255/132‑1 (MQ=255)
                                                                                                                     gCCA‑TCCACCGATTT‑GTCTGCTT‑CGCGGACGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAg           <  2:6128784/137‑1 (MQ=255)
                                                                                                                      ccA‑TCCACCGGTTT‑TTTTTTTT‑CGCGGGCGCCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc          <  1:16896409/137‑1 (MQ=255)
                                                                                                                      ccA‑TCCACCGGTTT‑TTCTGCCT‑CGCGGGAGCGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc          <  2:10513410/137‑1 (MQ=255)
                                                                                                                      ccA‑TCCACCGGGTG‑TTTTGCTT‑CGTGTGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc          <  2:9349977/137‑1 (MQ=255)
                                                                                                                       ccccccACCGGTTT‑GTCTTCTC‑CGGGTACAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc          <  1:14372927/133‑1 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  1:6309885/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  1:12361504/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  2:13028763/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  1:2993076/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  1:2993034/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  2:7670016/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  1:2519758/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         >  1:12361543/1‑137 (MQ=255)
                                                                                                                       cA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCAGCATCATGGGCAAGcc         >  2:15197626/1‑137 (MQ=255)
                                                                                                                        cgtCCACCGATTT‑TTCTTCTT‑CGTGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc         <  2:16281486/135‑1 (MQ=255)
                                                                                                                            cgcggAGTT‑GGCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATg     <  1:3596974/133‑1 (MQ=255)
                                                                                                                             cccGATTT‑TTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    <  2:12366644/136‑1 (MQ=255)
                                                                                                                             aCCGATTT‑TTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    <  2:12366643/137‑1 (MQ=255)
                                                                                                                             aCCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    >  1:8132963/1‑137 (MQ=255)
                                                                                                                             aCCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    >  1:131030/1‑137 (MQ=255)
                                                                                                                             aCCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    >  1:8132934/1‑137 (MQ=255)
                                                                                                                             aCCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    >  2:766006/1‑137 (MQ=255)
                                                                                                                             aCCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    >  1:401932/1‑137 (MQ=255)
                                                                                                                             aCCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg    >  2:3212223/1‑137 (MQ=255)
                                                                                                                              ccGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGc   >  2:16289710/1‑137 (MQ=255)
                                                                                                                              ccGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGc   >  1:2642816/1‑137 (MQ=255)
                                                                                                                               cGATTT‑TTCTGCTT‑TGCGGGCAACCCCGCTCCCACAGGGGGCCAAAGTTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCt  <  1:9102053/137‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                        
AGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCC‑ACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCA‑TCCACCGAGTT‑GTCTGCTT‑CGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCT  >  AE015451_phaM1/4889496‑4889757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑