Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,737,7560AG34.4% 261.2 / 89.7 224intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
Reads supporting (aligned to +/- strand):  ref base A (48/99);  new base G (0/77);  total (48/176)
Fisher's exact test for biased strand distribution p-value = 7.28e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

TGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  AE015451_phaM1/5737621‑5737892
                                                                                                                                         |                                                                                                                                         
tGGGCAATGGGTGGTGCACCAGGTGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGGACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAg                                                                                                                                          <  1:14040918/137‑1 (MQ=255)
        gggTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGTGTTTCAACCCCTCTCGTGTAAAGCCACGAAAGGGTTc‑ca                                                                                                                                  >  2:18099646/1‑137 (MQ=255)
        gggTGGTGCACCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGTTTTTGCGCCAGCTTTTGGGCG‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTg‑aaa                                                                                                                                 <  2:9060630/137‑1 (MQ=255)
            ggtgCGCCAGGGGCGGCATGCCAAGGAAGAGGAAGGTGGGCGGGCGTTGGCCAGGTTTTTGCCCCAGTTTTTGGGTT‑GAGCGTACGGGGGTTGCGTTGCAGCCTTTCGTGGGCAAGCCCGCG‑AGGGGTTa‑caacgca                                                                                                                             <  2:15462214/137‑1 (MQ=255)
                         tggcTTGCCGAGGAAGATGAAATTGGGCGGGCGTTTGCCAAGGTGTTTCGCCGGTTTTTGGATT‑GAGCTTACTGGGGTTGTGTTGCGTCCTTTCGTGTGCAATCCCGCG‑AGGGGTGG‑AAACGCAACCCCAAAAAACt                                                                                                                <  2:1056789/136‑1 (MQ=255)
                         aggcGTGCCAAGGAAGAGGAAAGCGGGCGGGCGTTGGCAAAGTTGTTGCGCCAGCTTTGGGGCT‑GAGTGTACTGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACt                                                                                                                <  2:9302969/136‑1 (MQ=255)
                           gcATTCCAAGGAGGATGAAGGTGGGTGGGTGTTTGCTAGGGGGTTGCGCCGGTTTTGGGGTG‑GAGCGGATGGGGGTGGTGTTGCAGCCCTTTGTGGGAAATCCCGCG‑AGGGGTGG‑AAACGCAACCCCAAAAAACTCa                                                                                                              <  2:4331561/137‑1 (MQ=255)
                           gcATACCAAGGAAGGGGAAAGCGGGGGGGTGTTTGCCAGGGTGTTGCGCCAGTTTTTGGGCG‑GAGTGTACAGGGGTTGCGTGGCAGCCCTTCGCGGGAAAGCCCGCG‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCa                                                                                                              <  2:1835579/137‑1 (MQ=255)
                                                    gggttttttGCAAGGTTGTTGCGCCATTTTTGGGGTG‑GAGTGTATTGGGGTGGTGTTGCATCCTTTAGGGGGAAACCCCGAG‑AGGGGTGA‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCg                                                                                     <  1:2608860/131‑1 (MQ=255)
                                                     gggtgTTTGCAGAGGTTTTGCGCCAGCTTTGGGGTG‑AAGGGTACTGGGGTTGTGTTGCATCCTTTCGCGGAAAAGCCCGCG‑AGGGGTTA‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  1:17257928/137‑1 (MQ=255)
                                                     gggctTTTGCAAGGGTTTTACGCCGTTTTTTGGGTT‑GAGCGTACTGGGTTTGTGTTGCACCCCTTCGCGTAAAATCCCGAG‑AAGGTTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  1:14511443/137‑1 (MQ=255)
                                                     gggagTTTGCCAAGGTGTTGCGCAAGCTTTGGGTTG‑GAGCGTACTGGGTTTTCGTTGCAGCCCTTCTCGGGAAAGCCCGTG‑AGGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCgg                                                                                    <  1:17867288/137‑1 (MQ=255)
                                                                 agttgtTGCGCCAGCTTTTGGGTG‑GAGCGTACCGGGGTTGCGTGGCAGCCCTTCTTGGGCAAGCCCGCG‑AAGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGa                                                                        <  2:18138733/134‑1 (MQ=255)
                                                                   gtgtTGCTCCGTTTTTTGGGCT‑GAGTGTACTGGGTTTGCGTGGCATCCCTTCGCGTGCAACCCCGCG‑AGGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATc                                                                      <  2:2535008/137‑1 (MQ=255)
                                                                    tgtTGCGCCAGCTTTTGGGCG‑GAGCGTATCGGGGTTGTGTGACAGCCCTTTGCGTGCAAGCCCGCG‑AGGGGTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCa                                                                     <  2:15718980/137‑1 (MQ=255)
                                                                       tGCTCCAGTTTTTGGGCT‑GAGCGCACCGGGGTTGTGTTGCATCCCTTCGCGGGCAAGCCCGTG‑AAGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:17088225/137‑1 (MQ=255)
                                                                       tGCTCCAACTTTTGGGTT‑AAGTGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:3891174/137‑1 (MQ=255)
                                                                       tGCGCCAGTTTTTGGGTG‑GAGCGGACGGGGGTTGTGTGGCAGCCCTTAGTGTGAAAGCCCGGG‑AGGGGTTA‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:11021035/137‑1 (MQ=255)
                                                                       tGCGCCAGCTTTTGGGTT‑GGGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAATCCCGCG‑AGGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  2:9432608/137‑1 (MQ=255)
                                                                       tGCGCAAGTTTTGGGGTG‑GGGTGTACGGGGGTGGTGTTGAATCCTTTTGTGTGAAGGCCCGGG‑AGGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                  <  1:3729267/137‑1 (MQ=255)
                                                                        gcgcCATTTTTTGGGTG‑GAGCGTACTGGGGTTTCTTTGCATCCTTTCGCGTAAAAGCCCGCG‑AAGGTTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  1:13782673/137‑1 (MQ=255)
                                                                        gcgcCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCg                                                                 <  1:9211121/137‑1 (MQ=255)
                                                                         ctccggCTTTTGGGCT‑GAGCGTACCGGGTTTTTGTTGCAGCTCTTCGCTTGCAAGCCCGCG‑AAGGTTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:16187201/132‑1 (MQ=255)
                                                                         ctccAGTTTTGGGGCG‑GGGTGTGCTGGGGTTGTGTTGCGGCCCTTCGCGGGCAATCCCGCG‑AGGGGTTG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  1:8499369/135‑1 (MQ=255)
                                                                         cgcTAGCTTTTGGGCT‑GGGAGGACTGGGGTGTCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:15305549/137‑1 (MQ=255)
                                                                         cgcCATTTTTGGGGCT‑GGGCGTACCGGGGTTGTGTTGCAGCCTTTCGCGTGCAAGCCTGTG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCagcaaa                                                                <  2:6961383/137‑6 (MQ=255)
                                                                         cgcCATCTTTTGGGGT‑GAGCGTACGGGGGTTCCTTTGCACCCCTTCGCGTAAAGTCCCGAG‑‑AGGGTTGAAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  1:10163047/137‑1 (MQ=255)
                                                                         cgcCAGTTTTTGGGTT‑GGGCGTACTGGGGTTGCGTTGCAGCCTTTTGCGGGCAAGCCCGCG‑AGGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:18614029/137‑1 (MQ=255)
                                                                         cgcCAGTTTTGGGGCG‑GAGCGGACCGGGGTTGTGTTGCATCCTTTCGCGGGCAAGCCCGCG‑AGGGGGTG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:1631911/137‑1 (MQ=255)
                                                                         cgcCAGCTTTGGGGCG‑GAGCGTACTGGGGTTGTGTTGCAGCCCTTCGCGTGCAGGCCCGCG‑AAGGGTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  2:8363410/137‑1 (MQ=255)
                                                                          gcagtcTTTTGGGCG‑GAGTGGACGGGGGTGGCGTTGCATCCCTTCGCGGGAAATCCCGCG‑AAGGGTTG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  1:9887925/132‑1 (MQ=255)
                                                                          gcCAGTTTTTGGGCG‑AAGCGTATCGGGGTTGCGTGGCAGCCCTTCGCGTGCAGGCCCGCG‑AAGGGTTC‑CAACGCAACCCCAAAACACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                               <  2:7894785/137‑1 (MQ=255)
                                                                          ccccaattttGGGTTGGAGTGTGCTGGGTTTGCGTGGAATCTCTTTGTGTGCAAGCCCGCG‑AATTGTTG‑CAACGCAACCCCAAAAAATTCATTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCgg                                                                <  1:14919863/131‑1 (MQ=255)
                                                                                             tgtatgggggTGGCGTTGGGGCTTTTCGTGGGAAAGCCAGTG‑‑AGGGTTGAAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:15355695/131‑1 (MQ=255)
                                                                                             tggatgggggTGGTGTGGAAGCCTTTTGTGTGCAACCCAGCG‑AGGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:13485771/131‑1 (MQ=255)
                                                                                             tgTACTGGGGTTGTGTTGCGGCCTTTTTGGTGAAATCCCGCG‑AGGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  1:2834832/136‑1 (MQ=255)
                                                                                             tgTACTGGGGTTGCGTTGCATCCCTTCGCGTGCAAGCCCGCG‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:17200566/136‑1 (MQ=255)
                                                                                             tgTACGGGGGTTGTGTGGCAGCCTTTTGTGGGAAAGCCCGCG‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:6298401/136‑1 (MQ=255)
                                                                                             cgcactggggTTGCGTTGCAGCCTTTCGCGGGCAAGCCAGCG‑AAGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:4665863/131‑1 (MQ=255)
                                                                                             cGTACTGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:14755534/137‑1 (MQ=255)
                                                                                             cGTACCGGGGTTGTGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                             <  2:13277294/137‑1 (MQ=255)
                                                                                                actgggtTTGCGTTGCATCCTTTCGTGTGCAAGCCCGCG‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                          <  1:15964096/134‑1 (MQ=255)
                                                                                                  tggggTGGTGTTGAGGCCTTTCGTGTGAAAGCCCGCG‑AGGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  1:13852320/136‑1 (MQ=255)
                                                                                                  cGGGGTTGCGTTGCGTCCCTTCGCGTGCAAGCCCACG‑AAGGTTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  2:12364053/137‑1 (MQ=255)
                                                                                                  cGGGGTTGCGTTGCAACCCTTCTCGTGCAATCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                        <  2:10842959/137‑1 (MQ=255)
                                                                                                     ggTTGTGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc                                     <  2:11819182/137‑1 (MQ=255)
                                                                                                        tgttttgcAGCCCTTCGTGTGCAGGCCCGCG‑AGGGGTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                  <  1:11492594/133‑1 (MQ=255)
                                                                                                          tgttgcAGCCTTTCGCGGGAAAGCCCGAG‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                <  1:10420274/136‑1 (MQ=255)
                                                                                                          ctttgcAGCCCTTCGCGTGCAAGCCCGCG‑AAGGTTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                <  2:11961557/135‑1 (MQ=255)
                                                                                                           tttgcAGCCTTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCACACCCCTGCTGCCGCGCCCTGGAc                               <  2:17815736/136‑1 (MQ=255)
                                                                                                           tttgcAGCCCTTCCTGCCAAAACCCTCG‑AAGCGTTG‑CAACGCAACCCCAAAAAACTCATTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCACGCGGCTGGTGTTATACCCCTGCTGCCGCGcccggaac                               <  2:13367931/136‑6 (MQ=255)
                                                                                                           gttgcAGCCTTTTGTGGGAAGGCCCGGG‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                               <  2:14176259/137‑1 (MQ=255)
                                                                                                           gtggcAGCTCTTCGTGGGAAAACCCGAG‑AAGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                               <  2:5579850/134‑1 (MQ=255)
                                                                                                                  cccTTCGCGTGCAAACCCGCC‑AAGGGTTG‑CCACGCAACCCCAAAAAACTCAGTGCAATTTTCCTATTTGCTGATCGGAAGCCCGCCAGATCAAGTGGATGGTGTCATACCCCTGCCGCCGCGCCCTGGGCAGCAGGt                        >  2:14094712/1‑137 (MQ=255)
                                                                                                                    tttCGCGGGC‑AGCCCGGG‑GAGGGGTGACAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:1886040/136‑1 (MQ=255)
                                                                                                                    cTTCTCGTCCAAGCCTGCG‑AATGGTTT‑CACCGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:10905889/137‑1 (MQ=255)
                                                                                                                    cTTCGTGTGCAAGCCCGCG‑AGGGTTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:12744174/137‑1 (MQ=255)
                                                                                                                    cTTCGTGTGAAATCCTGAG‑AGGGGTTG‑CACCGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:7719414/137‑1 (MQ=255)
                                                                                                                    cTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                      <  1:4264218/137‑1 (MQ=255)
                                                                                                                       cgcgTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                   >  2:12620698/1‑137 (MQ=255)
                                                                                                                        gcgGGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                  <  2:3156390/137‑1 (MQ=255)
                                                                                                                          gtacAAGCCCGTG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:16215809/134‑1 (MQ=255)
                                                                                                                          gtacAAACCCGCG‑AAGGGTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:16786108/134‑1 (MQ=255)
                                                                                                                          gggaaaGCCCGTG‑AGGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:1355429/133‑1 (MQ=255)
                                                                                                                          gggCAAGCCCGCG‑AGGGGTGG‑AAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:3354168/135‑1 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AGGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                <  2:14931955/137‑1 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:2640052/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:15647689/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:16129453/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:5353735/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:7555943/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:9359978/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:14783596/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  2:11424081/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAAAGCAGGTTCTCACGc                >  1:16812426/1‑137 (MQ=255)
                                                                                                                          gTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGACAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                >  1:12480438/1‑137 (MQ=255)
                                                                                                                               aGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa           >  1:15749608/1‑137 (MQ=255)
                                                                                                                               aGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa           >  2:13068396/1‑137 (MQ=255)
                                                                                                                                tcccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg          <  2:13039714/136‑1 (MQ=255)
                                                                                                                                gaaagag‑AAGGGGTG‑CAAAGAAAAAACAAAAAAATCAGTGAAGGGTTACTATTTGCTGATCGGACGCCCGCAAGATCAAGAGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg          >  2:6545651/7‑137 (MQ=255)
                                                                                                                                gCCCGCG‑AGGGGTGG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg          <  2:7304078/137‑1 (MQ=255)
                                                                                                                                  ctgcg‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:5868613/135‑1 (MQ=255)
                                                                                                                                  ctacg‑AAGGTTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:6017235/134‑1 (MQ=255)
                                                                                                                                  ccGTG‑AGGGGTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:5255063/137‑1 (MQ=255)
                                                                                                                                  ccGTG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:3327239/137‑1 (MQ=255)
                                                                                                                                  ccGCG‑AGGGTTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:15945202/137‑1 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:11752161/137‑1 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:17807652/137‑1 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:2223509/137‑1 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        >  1:4414949/1‑137 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        >  1:5558678/1‑137 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  2:15499550/137‑1 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        >  2:1401222/1‑137 (MQ=255)
                                                                                                                                  ccGCG‑AAGGGTGA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc        <  1:10613535/137‑1 (MQ=255)
                                                                                                                                  cagcg‑AGGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTGCTCACGCTCCCAGGc        <  1:12966673/135‑1 (MQ=255)
                                                                                                                                    gcg‑AGGGTTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg      <  1:6793173/137‑1 (MQ=255)
                                                                                                                                    gcg‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg      <  2:12557928/137‑1 (MQ=255)
                                                                                                                                      g‑AAGGGTTA‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGc    <  1:12040437/137‑1 (MQ=255)
                                                                                                                                         aGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAg  >  1:7231894/1‑137 (MQ=255)
                                                                                                                                         aGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAg  >  2:12969871/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCT‑GAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTG‑CAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  AE015451_phaM1/5737621‑5737892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑