BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,210,823	0	C	G	27.4%	1332.6 / 209.4	581	intergenic (+13/+24)	ytnA CDS (Uncharacterized amino acid permease YtnA)/PP_1060 CDS (putative Glutamate synthase_ large subunit)	–/–
Reads supporting (aligned to +/- strand): ref base C (40/318); new base G (135/0); total (263/318)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 583 total aligned reads displayed.

TTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGA  >  AE015451_phaM1/1210688‑1210959

     ggATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑ttt                                                                                                                                       <  1:4450132/137‑1 (MQ=255)

       aTACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGC‑ttttt                                                                                                                                     >  2:7732820/1‑135 (MQ=255)

       aTACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGA‑ttttt                                                                                                                                     >  1:10085753/1‑135 (MQ=255)

        tACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGGGC‑tttttg                                                                                                                                    >  1:357035/1‑134 (MQ=255)

          cgcgTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGc                                                                                                                                 <  2:4662769/137‑1 (MQ=255)

          cgcgTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGc                                                                                                                                 <  1:14759224/137‑1 (MQ=255)

             ttttgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTGTATTACACGTTCCGCCTGGCGCCGAGAGACGCACAAGGTTTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGcc                                                                                                                                <  1:5750008/134‑1 (MQ=255)

             gtgtGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACAA‑TTTT‑CGGcccc                                                                                                                              >  2:13523386/1‑137 (MQ=255)

              tgtgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCa                                                                                                                             <  1:10414347/137‑1 (MQ=255)

              tgtgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCa                                                                                                                             <  2:144371/137‑1 (MQ=255)

              tgtgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCa                                                                                                                             <  2:12623264/137‑1 (MQ=255)

              tgtgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCa                                                                                                                             <  1:4753425/137‑1 (MQ=255)

              tgtgGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCa                                                                                                                             <  1:16210901/137‑1 (MQ=255)

                  gCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGt                                                                                                                         <  2:15141631/137‑1 (MQ=255)

                      tGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTtctc                                                                                                                     <  1:8778254/137‑1 (MQ=255)

                          cATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGC‑TTTGTTGGCCCCA‑CGTtctct‑cct                                                                                                                >  1:783537/1‑135 (MQ=255)

                          cATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAAC‑TTTT‑TGGCCCCAACGTtctct‑ccg                                                                                                                >  2:16247400/1‑135 (MQ=255)

                          cATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACGA‑TTTG‑TGGTCCCAACATtttct‑ccg                                                                                                                >  1:11358750/1‑135 (MQ=255)

                           aTCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAgg                                                                                                               <  1:16781987/137‑1 (MQ=255)

                            tCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGC‑TTTT‑TGGCCCCCACCTtctct‑ccagc                                                                                                              >  2:1672933/1‑133 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGTAGC‑CTTT‑CGGTCCCCGCATTCTCT‑CCAgccgcc                                                                                                          >  1:16405230/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGT‑TTTT‑TTGCCCCCCCCTTCTCT‑CCGgccccc                                                                                                          >  2:2562108/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGC‑TTTT‑TGGCCCCCACGTTCTCT‑CAAgccgcc                                                                                                          >  1:12334212/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGC‑TTTT‑TGGCCCCAGCGTTCTCT‑CAGgccgcc                                                                                                          >  2:5795606/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGA‑TTTT‑TTGCCCCAGCGTTCTCT‑CCGgccgcc                                                                                                          >  1:14976803/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGA‑CTTT‑TGGCCCCAACGTTCTCT‑CCGgccgcc                                                                                                          >  1:15447768/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTT‑CGGCCCCAGCGTTCTCT‑CAGgccgcc                                                                                                          >  2:11235003/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACAC‑TTTG‑TTGCCCCCGCGTTTTCT‑CCGgccacc                                                                                                          >  1:13067171/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGAAGC‑TTTT‑CGGCCCCCGCGTTCTCT‑CCGgccgcc                                                                                                          >  2:7291828/1‑137 (MQ=255)

                                cccGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGAAGC‑TTTG‑TGGACCCAACGTTTTCT‑CCggtcacc                                                                                                          >  1:13646988/1‑132 (MQ=255)

                                  cGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCAC‑TTTG‑TGGCCCCAACGTTTTCT‑CCGGCcaccac                                                                                                        >  2:108629/1‑137 (MQ=255)

                                  cGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCAA‑TTTG‑TGGTCCCCACGTTCTCT‑CAAGCAACCAc                                                                                                        >  2:5333237/1‑137 (MQ=255)

                                  cGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACAC‑TTTT‑CTGCCCCCGCGTTTTCTCCAGgcccccc                                                                                                         >  2:6679999/1‑136 (MQ=255)

                                  cGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGAAGC‑TTTT‑TGGTCCCAGCGTTTTCT‑CCGgccccccc                                                                                                        >  1:12054089/1‑135 (MQ=255)

                                   ggCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACt                                                                                                       <  2:1734291/137‑1 (MQ=255)

                                     cGTTCCTGGTGTTGCTGACAGTGC‑‑GGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGGCCTGACGTGGGGTCGG‑TTTT‑TGGGTACAGCGTTTTTT‑CGGGCCGTCACTTc                                                                                                     >  2:18360383/1‑137 (MQ=255)

                                        tCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCt                                                                                                  <  2:6873856/137‑1 (MQ=255)

                                        tCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCt                                                                                                  <  2:11974565/137‑1 (MQ=255)

                                        tCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCt                                                                                                  <  1:8336556/137‑1 (MQ=255)

                                        tCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCt                                                                                                  <  1:31480/137‑1 (MQ=255)

                                          cTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAGC‑TTTT‑CGGCCCCCACACTCTCT‑CCAGCCGCCACCTCGCttt                                                                                                >  2:819964/1‑135 (MQ=255)

                                                     gACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGC‑TTTT‑TTGTCCCCACTTTTTCT‑CCGGCCTCCACTTCTCTTTTCGGATCTaaa                                                                                     >  1:15328239/1‑137 (MQ=255)

                                                     gACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGAAGA‑TTTT‑TGGCCCCCGCTCTCTCT‑CCGGCTACCCCTTCGGTGTGTGGCtttaaa                                                                                     >  2:343878/1‑132 (MQ=255)

                                                      aCAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAAc                                                                                    <  2:15629346/137‑1 (MQ=255)

                                                           gC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGTAAC‑TTTG‑TGGGCCCAACGTTCTCT‑CAAGCCGCCCCCTCTCCGTTTAGCTCGAAAACGTcc                                                                               >  2:4511287/1‑137 (MQ=255)

                                                           gC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTcc                                                                               <  2:9488491/137‑1 (MQ=255)

                                                           gC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTcc                                                                               <  1:13316298/137‑1 (MQ=255)

                                                           gC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCAGC‑TTTG‑CGGCCCCCACGTTCTCT‑CAAGCCGCCACTTCGCTGTGTGGATCGAAACTGttc                                                                               >  2:10992604/1‑135 (MQ=255)

                                                           gC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCAGA‑TTTG‑CGGTCCCCACGTTCTCT‑CAGGCCGACACCTCGCTGTGCGGATCTAAACCGttc                                                                               >  2:3305499/1‑135 (MQ=255)

                                                                  aTTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCg                                                                         <  1:18281207/137‑1 (MQ=255)

                                                                         gTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTT‑CTGCCCCAGCGTTTTCT‑CCGGCCGCCACCTCGCTTTGTGGATCTAAAATGTCCT‑CCCAGGACaag                                                                  >  2:1473613/1‑135 (MQ=255)

                                                                         gTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑CTTG‑CTGTCCCCACCTTCTCT‑CCGGTCGCCACCTCTCTTTGCGGGTCGAAAATGTCCT‑CCCCGGACAGt                                                                  >  2:1224370/1‑137 (MQ=255)

                                                                           tCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTg                                                                <  1:14140428/137‑1 (MQ=255)

                                                                            ccGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAAG‑TTTG‑TTGCCCCAGCGTTCTCT‑CCAACCCCCCCCTCGCCTTTCGGCTCCACAATCTCCG‑CCCCGGcccatttc                                                               >  2:2848206/1‑131 (MQ=255)

                                                                             cGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGcc                                                              <  2:18170972/137‑1 (MQ=255)

                                                                              gcatGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCAC‑TTTT‑CGGCCCCCACGTTCTCT‑CCAGCCGCCCCCTTGCCGTGCGGATCGAAAACGTCCA‑CCAAGGCCAATTGTca                                                             >  2:17915606/4‑137 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGTCGC‑TTTT‑CGGCCCCCACTTTCTCT‑CCGGCCGCCCCTTCTCTTTTTTGTTCTAAAATGTCCG‑CCCCGGCCAGTTgtcc                                                             >  2:8488472/1‑134 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGGGC‑CTTT‑TGGTCCCCACGTTTTCT‑CCGGCCCCCCCTTCGCTTTGTGGGTCGGAAATGTCCA‑CCCGGGACAGGTGTca                                                             >  1:12270160/1‑137 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGA‑TTTT‑TGGCCCCCACGTTCTCT‑CCAGCCGCCCCCTCTCTGTTCTGCTCCAAACTGTCCC‑CCCCGGGCAAttttca                                                             >  2:13396652/1‑133 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAGCTTTTT‑TTGCCCCA‑CGTTTTCT‑CCAGCCCCCACCTCCCTTTTTTGCTCTAAACCGTTCGCCCCGGGCCATTTTcc                                                              >  2:11728506/1‑137 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAGC‑TTTG‑TGGCCCCAACGTTCTCT‑CCAGCCACCACCCCGCTTTTCGGATTGAAAACGTCCG‑CCCGGGGCAGTTTccc                                                             >  2:71590/1‑136 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGAAA‑TTTT‑TGNTCCCCACATTTTCC‑CCGGACGCCCCCTCGTTGTGTGGGTCGGAACGGTCTG‑CCCAGGTCAGTTgtcc                                                             >  1:16077607/1‑134 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTT‑CGGCCCCAGCGTTCTCC‑CCAGCCGCCACCTCGCTGTGCGGTTCGAAACTGTCCG‑CCCGGGCCAGTTGCca                                                             >  2:10392013/1‑137 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACGC‑TTTG‑CGGCCCCCACGTTCTCT‑CCGGCCGCCACCTCGCTGTGTGGCTCGAAACTGTCCG‑CCCGGGCCAGTTTCca                                                             >  2:16415127/1‑137 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACAC‑TTTT‑CGGCCCCCACGTTCTCT‑CCAGCCGCCACCTTGCTGTTCGGCTCGAAAACGTCCG‑CCCAGGCCAGTTTccc                                                             >  2:14302193/1‑136 (MQ=255)

                                                                              gCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGACAC‑TTTG‑CGGTCCCCGCATTCTCT‑CCGGCCGCCACCTCACTTTGTGGCTCGAAAATGGCCG‑CCCGGGACAGTTGCca                                                             >  2:7439789/1‑137 (MQ=255)

                                                                               ccTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCcac                                                            <  2:3785015/137‑1 (MQ=255)

                                                                                cTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTTGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCcaca                                                           <  1:8114079/137‑1 (MQ=255)

                                                                                cTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CATGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCcaca                                                           <  2:6430238/137‑1 (MQ=255)

                                                                                cTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTCTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCcaca                                                           <  1:17945035/137‑1 (MQ=255)

                                                                                     gcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCcgc                                                      <  2:17056892/137‑1 (MQ=255)

                                                                                     gcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCcgc                                                      <  1:15602370/137‑1 (MQ=255)

                                                                                     gcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCcgc                                                      <  2:17678448/137‑1 (MQ=255)

                                                                                     gcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCcgc                                                      <  2:6132102/137‑1 (MQ=255)

                                                                                            gACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGa                                               <  2:1591002/137‑1 (MQ=255)

                                                                                            gACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGa                                               <  2:10017415/137‑1 (MQ=255)

                                                                                                   aaGGTGTGATGAGTACAGCCGCCCGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGcc                                        <  2:4788920/137‑1 (MQ=255)

                                                                                                   aaGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGcc                                        <  2:13175809/137‑1 (MQ=255)

                                                                                                   aaGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCAC‑TTTG‑CGGCCCCAGCGTTCGCT‑CAGGCCGCCACTTCGCTGTACGACTCGAAACTTTCCG‑CCCTGGCCAGTTGCTACATCCGCGAATAGAACTGAcc                                        <  2:5116998/137‑1 (MQ=255)

                                                                                                        gtgACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCa                                   <  2:10655559/137‑1 (MQ=255)

                                                                                                          gACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCAcc                                 <  1:9143135/137‑1 (MQ=255)

                                                                                                          gACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCAcc                                 <  1:18611116/137‑1 (MQ=255)

                                                                                                          gACGAGTACAGCCTCCTGACCTGACGTGGGGAAGC‑TTTT‑TAGTCCCCACGTTCTCC‑CCAGCCCCCACCTCCCCATGTGGGTCTAAACTGTTCA‑CCCCGGCCAATTGCCACATCCGCAAACGAAAATGGCCGTTcccc                                 >  2:14963874/1‑137 (MQ=255)

                                                                                                                tACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCg                           <  1:2574556/137‑1 (MQ=255)

                                                                                                                  cAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGt                         <  1:654568/137‑1 (MQ=255)

                                                                                                                     cctcctGACCTGACGTTGGGCCGC‑TTTG‑CGGCCCCAGTGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTcag                      <  1:11756426/137‑1 (MQ=255)

                                                                                                                     cctcctGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTcag                      <  2:4113504/137‑1 (MQ=255)

                                                                                                                     cctcctGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTcag                      <  1:3635315/137‑1 (MQ=255)

                                                                                                                      ctcctGACCTGACGTGGGGACGC‑TTTT‑CGGCCCCAACATTCTCT‑CCAGCCACCCCCTCTCTTTTTGGTTTGAAACCGTCCG‑CCCAGGGCAATTGCCACACCCGCGAACCGAACCGGCCATTCCCCCCGCCAGTcagc                     >  2:8965752/1‑137 (MQ=255)

                                                                                                                        cctgacctgaCGTGGGGCCGC‑TTTG‑TGTCCCCAGCTTTCCCT‑CAGGCCGCCACCCCGCCGTGTGGCTCGGAACTGTCCA‑CCCGGGCCAGCTGCCACATCCGAGAATCGACCTGGCCGTTTCCCTCTCCGGTcagaag                   >  2:16069126/1‑137 (MQ=255)

                                                                                                                          tgacctgacTTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCGCGCCGGTCAGCAGCt                 <  1:9793260/137‑1 (MQ=255)

                                                                                                                           gacctgacGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTc                <  1:6128386/137‑1 (MQ=255)

                                                                                                                           gacctgacGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTc                <  2:9670935/137‑1 (MQ=255)

                                                                                                                            acctgacGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCa               <  1:17683798/137‑1 (MQ=255)

                                                                                                                             cctgacGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCAc              <  2:1856074/137‑1 (MQ=255)

                                                                                                                               tgacGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCt            <  1:13788745/137‑1 (MQ=255)

                                                                                                                                  cGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGc         <  2:13845848/137‑1 (MQ=255)

                                                                                                                                  cGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGc         <  1:17373668/137‑1 (MQ=255)

                                                                                                                                   gTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTTCCACATCCGCTAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCt        <  2:12771686/137‑1 (MQ=255)

                                                                                                                                    tGGGGACAC‑TTTT‑CGGCCCCCACGTTTTCT‑CCGGCCGCCACTTCGCTTTGCGGCTCGAAACTGTCCA‑CCCGGGCCAATTTCCCCACCCTCGAATAAAACCGGCCATTCCCCCCGCCAATCAACAGCCCACCCTGCtt       >  2:8766135/1‑137 (MQ=255)

TTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGC‑‑TGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGC‑TTTG‑CGGCCCCAGCGTTCTCT‑CAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGA  >  AE015451_phaM1/1210688‑1210959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`