BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	542,514	C→G	L252V (CTG→GTG)	rpoC CDS (DNA‑directed RNA polymerase subunit beta') →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	542,514	0	C	G	100.0%	2697.1 / NA	680	L252V (CTG→GTG)	rpoC CDS (DNA‑directed RNA polymerase subunit beta')
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (421/259); total (421/259)

Only 100 of 691 total aligned reads displayed.

GACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACG  >  AE015451_phaM1/542381‑542643

 aCCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCggtggt                                                                                                                                    >  2:10762069/1‑137 (MQ=255)

 aCCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCggtggt                                                                                                                                    >  1:8531799/1‑137 (MQ=255)

  ccAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGtt                                                                                                                                   <  1:8113249/137‑1 (MQ=255)

   cAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTc                                                                                                                                  <  1:6562325/137‑1 (MQ=255)

    aaCTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTcc                                                                                                                                 >  2:11552198/1‑137 (MQ=255)

      cTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGc                                                                                                                               >  1:7234301/1‑137 (MQ=255)

              ccAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGAtggt                                                                                                                       >  2:6352856/1‑137 (MQ=255)

              ccAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGAtggt                                                                                                                       >  2:17522952/1‑137 (MQ=255)

              ccAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGAtggt                                                                                                                       >  1:13081680/1‑137 (MQ=255)

              ccAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCCTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGAtggt                                                                                                                       >  2:6649038/1‑137 (MQ=255)

              ccAAGATCAAGAAGCTTTACAAGCGTCTGAAGCTGATGGAGGCTTTCCAGGGCTCAGGCAACCTGCCCGAGTGGATGGTCCTGAACGTCCTGCCAGTGCTGCCGCCGGACCTGCATCCGGTGGTTCCGCTGGAtggt                                                                                                                       >  2:988331/1‑137 (MQ=255)

                    tCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGt                                                                                                                 >  2:7215663/1‑137 (MQ=255)

                      aagaagCTTTCCAAGCGTTTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGttt                                                                                                               <  2:7040029/137‑1 (MQ=255)

                      aagaagCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGttt                                                                                                               <  1:16837355/137‑1 (MQ=255)

                      aagaagCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGttt                                                                                                               <  1:1668765/137‑1 (MQ=255)

                           gCTTTCCAAGCGTCTGAAGCTGATGGACGCTTTCCAGGGCTCGGACAAACTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGATGGATGGTGGCAGTGTcgcgg                                                                                                          >  2:4540811/1‑136 (MQ=255)

                           gCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGa                                                                                                          <  2:16470765/137‑1 (MQ=255)

                                 cAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCg                                                                                                    >  2:18097365/1‑137 (MQ=255)

                                       tCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGa                                                                                              <  2:16829835/137‑1 (MQ=255)

                                         tGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑c                                                                                       <  1:13481151/137‑1 (MQ=255)

                                            aGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGAc                                                                                    >  2:2455139/1‑137 (MQ=255)

                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCTTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATc                                                                             <  1:17313655/137‑1 (MQ=255)

                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATc                                                                             <  2:12430775/137‑1 (MQ=255)

                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATc                                                                             <  1:9240953/137‑1 (MQ=255)

                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATc                                                                             <  1:7445856/137‑1 (MQ=255)

                                                    gAAGCTTTCCAGGGCTCGGGCACCCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGAATGGTGGCCGTTTCGCGACTTCCGAACTGAA‑‑‑‑‑CGACCTTTATCg                                                                            >  2:3807351/1‑137 (MQ=255)

                                                    gAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCg                                                                            >  2:6150204/1‑137 (MQ=255)

                                                     aaGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGc                                                                           >  1:12022106/1‑137 (MQ=255)

                                                          ttCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGt                                                                      <  2:3279478/137‑1 (MQ=255)

                                                           tCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTg                                                                     <  2:10189610/137‑1 (MQ=255)

                                                            ccAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGa                                                                    <  1:7883951/137‑1 (MQ=255)

                                                              aGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATc                                                                  >  1:3914099/1‑137 (MQ=255)

                                                              aGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATc                                                                  <  1:15898600/137‑1 (MQ=255)

                                                                     gggCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTa                                                           <  2:15543836/137‑1 (MQ=255)

                                                                     gggCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTa                                                           <  1:4661711/137‑1 (MQ=255)

                                                                     gggCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTa                                                           <  2:14258643/137‑1 (MQ=255)

                                                                     gggCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTa                                                           >  1:14437432/1‑137 (MQ=255)

                                                                        cAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTaaca                                                        >  2:2591775/1‑137 (MQ=255)

                                                                        cAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTaaca                                                        >  1:18252114/1‑137 (MQ=255)

                                                                        cAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTaaca                                                        >  2:7508/1‑137 (MQ=255)

                                                                        cAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTaaca                                                        >  1:3147136/1‑137 (MQ=255)

                                                                        cAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTaaca                                                        >  1:15203506/1‑137 (MQ=255)

                                                                             tgcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGt                                                   >  2:11042017/1‑137 (MQ=255)

                                                                             tgcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGt                                                   >  2:18637453/1‑137 (MQ=255)

                                                                              gcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTc                                                  <  2:17218890/137‑1 (MQ=255)

                                                                              gcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTc                                                  >  1:14887264/1‑137 (MQ=255)

                                                                                ctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTg                                                <  2:2194076/137‑1 (MQ=255)

                                                                                  gAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGaa                                              >  2:14954085/1‑137 (MQ=255)

                                                                                  gAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGaa                                              <  1:5198365/137‑1 (MQ=255)

                                                                                     tGGATGGTCCTGACCGTCCTGCCCGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCAGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAgcg                                           >  2:5872391/1‑137 (MQ=255)

                                                                                        aTGGTCCTGACCGTCCTGCCGGTGCTGTCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTACCAACCGTCTGAAGCGCct                                        >  2:17016449/1‑137 (MQ=255)

                                                                                        aTGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCct                                        >  2:5023993/1‑137 (MQ=255)

                                                                                        aTGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCct                                        >  2:15239152/1‑137 (MQ=255)

                                                                                        aTGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCct                                        >  2:3995884/1‑137 (MQ=255)

                                                                                           gTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTAGCCGTTTCACGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCctgct                                     >  1:5442384/1‑137 (MQ=255)

                                                                                            tCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGa                                                                    >  2:18352104/1‑105 (MQ=255)

                                                                                            tCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCctgctg                                    >  2:557042/1‑137 (MQ=255)

                                                                                             ccTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTgg                                   >  1:207899/1‑137 (MQ=255)

                                                                                                gACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATc                                <  1:1706179/137‑1 (MQ=255)

                                                                                                gACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATc                                <  2:13255147/137‑1 (MQ=255)

                                                                                                   cGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGt                             <  1:12288419/137‑1 (MQ=255)

                                                                                                    gTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                            >  2:13442515/1‑137 (MQ=255)

                                                                                                    gTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                            >  1:6037949/1‑137 (MQ=255)

                                                                                                    gTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                            >  2:8183996/1‑137 (MQ=255)

                                                                                                    gTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                            >  1:11686399/1‑137 (MQ=255)

                                                                                                    gTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                            >  1:12803956/1‑137 (MQ=255)

                                                                                                     tCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCg                           <  1:15041932/137‑1 (MQ=255)

                                                                                                     tCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCg                           <  2:13554319/137‑1 (MQ=255)

                                                                                                     tCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCg                           <  2:12199277/137‑1 (MQ=255)

                                                                                                     tCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCg                           <  1:5727536/137‑1 (MQ=255)

                                                                                                     tCCTGACAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCATAACAACCGTCTGAAGCGCCTGCTGGATCTGTCg                           <  1:17693434/137‑1 (MQ=255)

                                                                                                      ccTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCgg                          >  2:14523378/1‑137 (MQ=255)

                                                                                                       cTGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgc                         <  1:6861265/137‑1 (MQ=255)

                                                                                                        tGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcg                        >  1:3628245/1‑137 (MQ=255)

                                                                                                        tGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcg                        >  2:8807223/1‑137 (MQ=255)

                                                                                                        tGCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcg                        >  2:2869015/1‑137 (MQ=255)

                                                                                                         gCCATTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:6148747/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGCCCTGTATCGCCGGGTGATCAACCGTCACACCCGTCTGACGCGCCTGCTGGATCTGTCGgcgc                       >  1:8773730/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgt                       >  1:10905292/1‑136 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:2392303/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:294654/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:10309843/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:4900027/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  2:773950/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:16186647/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  2:9320052/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:10020177/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:4289229/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  2:15973823/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  2:10400195/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  1:11166288/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  2:14035653/1‑137 (MQ=255)

                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                       >  2:14772943/1‑137 (MQ=255)

                                                                                                                ctgcCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGAcat                >  2:4657677/1‑137 (MQ=255)

                                                                                                                ctgcCGCCGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGAcat                >  2:17449278/1‑137 (MQ=255)

                                                                                                                  gccgccGGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGAcatca              >  1:9617199/1‑137 (MQ=255)

                                                                                                                   ccgccgGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGAcatcat             >  1:4416463/1‑137 (MQ=255)

                                                                                                                    cgccgGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACatcatc            >  1:275972/1‑137 (MQ=255)

                                                                                                                     gccgGACCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCg           <  2:13781728/137‑1 (MQ=255)

                                                                                                                          aCCTGCGTCCGGTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCCGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCg           <  2:8386593/137‑1 (MQ=255)

GACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAA‑‑‑‑‑CGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACG  >  AE015451_phaM1/542381‑542643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`