BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	1,713,060	A→G	T2A (ACC→GCC)	tsaB CDS (endopeptidase) →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,713,060	0	A	G	99.9%	3274.9 / NA	808	T2A (ACC→GCC)	tsaB CDS (endopeptidase)
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (450/356); total (452/356)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 811 total aligned reads displayed.

ACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712929‑1713192

aCGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGgccacc                                                                                                                                 <  2:7296053/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGTTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:11263094/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:14899978/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  2:9367139/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  2:15838792/137‑1 (MQ=255)

 cGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCAccc                                                                                                                                <  1:7532488/137‑1 (MQ=255)

   cACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCctg                                                                                                                              <  2:15233327/137‑1 (MQ=255)

       tCGACAACTGTCCGCTTGCGGGCCTTTGTCGTTTGTATGCCACACAGCGCCCCCGCAGGACACAGCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTgg                                                                                                                          <  2:12292918/137‑1 (MQ=255)

             aCGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTgg                                                                                                                    >  1:199506/1‑137 (MQ=255)

               ggCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATCCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGAt                                                                                                                  >  1:10369330/1‑137 (MQ=255)

                gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATa                                                                                                                 <  1:15640670/137‑1 (MQ=255)

                   cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCg                                                                                                              >  2:3484003/1‑137 (MQ=255)

                   cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCg                                                                                                              >  2:2001833/1‑137 (MQ=255)

                   cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCg                                                                                                              >  2:5404051/1‑137 (MQ=255)

                   cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCg                                                                                                              <  1:7022910/137‑1 (MQ=255)

                   cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCAATCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCg                                                                                                              >  1:8804615/1‑137 (MQ=255)

                   cGCCTGCTGGCCATTGTCGTTTGTAGGCCTCACAGCGCCCCCTCAGGACACAACACCGCCTCTGCTCTATACCGCCGCTCTTTTTCACTCGCACCCGGATACCCATTCTATGGCCACCCTGCTGGCCCTGGATAcca                                                                                                              >  1:12436904/1‑136 (MQ=255)

                       tGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAc                                                                                                          <  2:17330052/137‑1 (MQ=255)

                        gCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCCTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAcc                                                                                                         >  2:7802190/1‑137 (MQ=255)

                        gCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCAcc                                                                                                         >  2:18262880/1‑137 (MQ=255)

                          gggCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCATGACACAACGCCGCTGCTGCTCTATATTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGa                                                                                                       <  2:16336842/137‑1 (MQ=255)

                                   tCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTc                                                                                              >  2:2990308/1‑137 (MQ=255)

                                      tttGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGt                                                                                           <  2:1387615/137‑1 (MQ=255)

                                       ttGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTc                                                                                          <  2:7010303/137‑1 (MQ=255)

                                       ttGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTc                                                                                          <  2:5034366/137‑1 (MQ=255)

                                       ttGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTCTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTc                                                                                          <  2:2609766/137‑1 (MQ=255)

                                           aGGCCGCCCCGCGCCCCCTCAGGACACACCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCACAACCGAACACCCGCTCGCTGGCCACCCTGCTGGCCCTGGATCCCACCACCGAAGCCTGTTCCGTCgcgc                                                                                      >  2:12606676/1‑137 (MQ=255)

                                           aGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCgcgc                                                                                      >  2:6559332/1‑137 (MQ=255)

                                             gCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctg                                                                                    >  1:10756577/1‑137 (MQ=255)

                                             gCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctg                                                                                    <  2:12903977/137‑1 (MQ=255)

                                               cGCACAGCGCCCCCTCATGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCgctgct                                                                                  <  1:16306279/137‑1 (MQ=255)

                                                gCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGctgctg                                                                                 >  1:7225618/1‑137 (MQ=255)

                                                  accgcgcCCCCTCCGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCCTTTTCACTCGCACCTGAATCCCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCCCCGAAGCCTGTTCAGTCGCGCTGCTGCa                                                                               >  2:10880677/4‑137 (MQ=255)

                                                  acaGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCa                                                                               >  2:9000465/1‑137 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:15669841/1‑137 (MQ=255)

                                                    aGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATg                                                                             >  2:11541403/1‑137 (MQ=255)

                                                         ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  1:3142629/1‑137 (MQ=255)

                                                         ccccTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGc                                                                        >  2:4372250/1‑137 (MQ=255)

                                                              cAGGACACAACGCCGCTGCTGCTCTATGCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTTCTGGCCCTGAATACCACCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGt                                                                   <  2:8811496/137‑1 (MQ=255)

                                                               aGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTg                                                                  <  1:10947031/137‑1 (MQ=255)

                                                               aGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTg                                                                  <  2:14805700/137‑1 (MQ=255)

                                                                 gACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAc                                                                <  1:4069615/137‑1 (MQ=255)

                                                                 gACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAc                                                                <  1:6714082/137‑1 (MQ=255)

                                                                  acacAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGAcc                                                               <  1:17623416/137‑1 (MQ=255)

                                                                       aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  2:10205287/137‑1 (MQ=255)

                                                                       aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          <  1:13696611/137‑1 (MQ=255)

                                                                       aCGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          >  1:5983414/1‑137 (MQ=255)

                                                                        cgccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCAc                                                         >  1:804877/1‑137 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCCTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCACGCTGCTGCATGACGGCAAGGTGACCCGCCACt                                                        >  1:17000973/1‑137 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  2:14215836/1‑137 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  1:8501078/1‑137 (MQ=255)

                                                                         gccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACt                                                        >  2:2172364/1‑137 (MQ=255)

                                                                          ccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCa                                                          >  1:2569742/1‑134 (MQ=255)

                                                                          ccgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTa                                                       >  2:17963561/1‑137 (MQ=255)

                                                                           cgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTAc                                                      <  1:12756945/137‑1 (MQ=255)

                                                                           cgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTAc                                                      >  2:16732254/1‑137 (MQ=255)

                                                                           cgcTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTAc                                                      >  2:13441609/1‑137 (MQ=255)

                                                                            gctgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACg                                                     >  1:6311597/1‑137 (MQ=255)

                                                                             ctgctgctCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGa                                                    <  1:14585160/137‑1 (MQ=255)

                                                                                         aCTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCcgcg                                        <  2:4892895/137‑1 (MQ=255)

                                                                                           tGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCcgctca                                      <  1:11547805/137‑4 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  2:9732636/137‑1 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  1:16733240/137‑1 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  1:18022024/137‑1 (MQ=255)

                                                                                            gCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcat                                     <  1:4988664/137‑1 (MQ=255)

                                                                                             ccGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatg                                    >  2:8550079/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGCTACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:17242105/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:12008383/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:13826844/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:7650834/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:14076096/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:6212859/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:3857067/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:3379988/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:3779917/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  2:10686970/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:997626/1‑137 (MQ=255)

                                                                                                ctctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCgcatgcat                                 >  1:576386/1‑137 (MQ=255)

                                                                                                  ctTTTTCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCatgcatgc                               >  1:11796530/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:5406874/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:13355209/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:18302781/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  1:2292197/1‑137 (MQ=255)

                                                                                                   tttttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGcc                              >  2:17538295/1‑137 (MQ=255)

                                                                                                   ttcttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCAATCCGAGGTGATCCCGCACATGCATGcc                              >  1:17279981/4‑137 (MQ=255)

                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:1122856/1‑137 (MQ=255)

                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:2300857/1‑137 (MQ=255)

                                                                                                     tttCATTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCa                            >  2:14636987/1‑137 (MQ=255)

                                                                                                      tttaTTCGTACCTGGATGCCCGTTCGGTGGCCGCCCTGCTGGCCCTGGATACCGCCACCGAATCCTGTTCCGTCGCGCTGCTGCATGACGTCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAg                           <  2:6940372/134‑1 (MQ=255)

                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  1:10055201/137‑1 (MQ=255)

                                                                                                         aTTCGCACCTGGATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAg                        <  2:16976380/137‑1 (MQ=255)

                                                                                                                    gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCTAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  1:1590498/137‑1 (MQ=255)

                                                                                                                    gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  1:9200496/137‑1 (MQ=255)

                                                                                                                    gATACCCGTTCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCgat             <  1:4420963/137‑1 (MQ=255)

                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  2:10359820/1‑137 (MQ=255)

                                                                                                                            ttCGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGc     >  1:12127685/1‑137 (MQ=255)

                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  2:8122057/1‑137 (MQ=255)

                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   <  1:1536835/137‑1 (MQ=255)

                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  1:2877301/1‑137 (MQ=255)

                                                                                                                              cGATGGCCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCaa   >  2:4708319/1‑137 (MQ=255)

ACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCTGTTCCGTCGCGCTGCTGCATGACGGCAAGGTGACCAGCCACTACGAGGTGATCCCGCGCATGCATGCCCAGAAGCTGCTGCCGATGATCAAGCAAC  >  AE015451_phaM1/1712929‑1713192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`