| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 4,740,816 | T→G | intergenic (+53/+700) | gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 4,740,816 | 0 | T | G | 94.9% | 1250.1 / NA | 352 | intergenic (+53/+700) | gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase) | –/– |
| Reads supporting (aligned to +/- strand): ref base T (1/17); new base G (208/126); total (209/143) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 353 total aligned reads displayed.
AGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC > AE015451_phaM1/4740690‑4740949 | aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGTTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg > 2:6398721/1‑137 (MQ=255) cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc < 1:6153036/137‑1 (MQ=255) ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct < 1:1037505/137‑1 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTctc > 1:11859260/1‑135 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc > 2:3698335/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc > 2:17140632/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc > 2:16021307/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc > 2:15001985/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc > 2:5314656/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc > 1:14673659/1‑137 (MQ=255) gTCCGCGCCAGCTGTACACCAGCGAGCAGACGCGCGACATCGTTGCCCTGAAAGACCGCACGTAAGCTGCAGTAGCCGAACGCACGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGTCTCTTc‑gc > 1:8734779/1‑137 (MQ=255) gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCtt < 2:13895967/137‑1 (MQ=255) ccAGTTGTACACCGGCGAGAAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGGAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTGTTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTg < 1:6300109/137‑1 (MQ=255) cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTC‑GCGGGCTTGc > 2:1199626/1‑137 (MQ=255) tGTACACTGGCGGGCAGAAGCGTGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTACAGTAGCCGAATGCAAGAAGGCTGCCCTGGGGCAG‑CTTTTTTTATTTGTCCTGTGCTGGCCTTTTC‑GAGGCCTTGCCcgc < 2:2214204/137‑1 (MQ=255) tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCGGGAGCCGAACGCAGGAGGGCTGCCCTCGGGCAG‑CTTTTTTTGTTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCcgc < 2:11139391/137‑1 (MQ=255) cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCTCAAGTAGGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAag < 2:11764633/137‑1 (MQ=255) cacCGGAAAACAAAAGCACAACACCGTTCCCTGGAAAGACCGCAAGAAAGCGGAAGAAGCAGAACGAAAGAAGGCTCCCCTCGGGCAGCC‑TTTTTTATTTTTCTTGTTCCGGCCCCTTC‑GCGACTTTCCCCGCAAag < 2:574992/137‑1 (MQ=255) ccGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAATGCTTCCCTCTGGCAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAagag < 2:18161366/137‑1 (MQ=255) aaaaGAAGCGCAACATCGTTGCCCGGAAAGACCGCGAGTAGACTGCAGTAGCTGAACGCAAGAAGACTGCCCTTTGGCAG‑CTTGTTTGAGTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCTCGAGAAGAGGCCGGc < 1:5439268/134‑1 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCGGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:9433536/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGNCCTCGGGCAGCC‑TTTTTTACTTGTCCTGTGCCGGCCCCTTC‑GCAGCCTTGCCCACGAAGAggacaga > 1:16063972/1‑132 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑TCCGCCTTTCCCACGAAAAAGACGGc > 1:144932/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCTGTCCTTCCCACGAAGAGGcaaga > 1:1758725/1‑133 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGTCTTGCCCGCGAAGAGGCCggt > 1:18139433/1‑136 (MQ=38) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGGGGCCGGc > 2:5743630/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGGGGCCGGc > 1:9326775/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAgggcagc > 1:18049113/1‑132 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:9063054/1‑137 (MQ=39) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 1:12255686/1‑137 (MQ=39) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:2543638/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:17524121/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:13106257/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:14633338/1‑137 (MQ=39) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:15399210/1‑137 (MQ=39) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAAGccaga > 1:2795325/1‑134 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAAAGGCCGGc > 1:7882711/1‑137 (MQ=38) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCAAAGAGGCCGGc > 1:2640521/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTC‑GCGGCCTTTTCCGCGAAGAGGACGGc > 1:1000776/1‑137 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTC‑GCGGCCTTTCCCTCGAAAAggactgc > 2:4683165/1‑132 (MQ=255) aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGACTCTTC‑GCGTTCTTTTCCTCGCAGAgggaggg > 1:14172999/1‑132 (MQ=255) aGCAGAAGCGCGACACCGTTGCCCTGAAAGACCGCAAGTAAACTGCAGTAGCAGAACACAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc > 2:12474676/1‑137 (MQ=38) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCCAGTAAGCTGCAGTAGCCGAACGCAAGAGGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCTGCGAAGAGTCCGGcaca > 2:17352912/1‑137 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGccca > 1:13807086/1‑137 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGcaca > 1:18621798/1‑137 (MQ=39) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCACGAAAAGGCCGGcaca > 1:17062940/1‑137 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCACGAAAAGGACGGaaca > 1:9417507/1‑137 (MQ=255) tCGTTGCCCTGAAAGACTGCAAGTAAGCTGCAGTGGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTACCCGCGAAGAGGCCGGCACAGACACCACaaaa < 2:5158430/137‑1 (MQ=255) aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct < 2:5643664/137‑1 (MQ=255) gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc > 2:131433/1‑137 (MQ=255) gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTC‑GCGGCCTTGCCCGCGAAAAGGCCGGCACAAACACCCCAAAACCCCCATTTTtctc > 1:10845093/1‑137 (MQ=37) aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc < 2:8195338/137‑1 (MQ=255) cacAAGTAAGCTGCAGGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCGGTGCCGGCCTCTTT‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc < 1:9114565/135‑1 (MQ=255) cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGTC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCTGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc > 2:18414651/1‑137 (MQ=255) cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGACCTCTTC‑GCAGACTTGCCCGCGAAGAGGCCAGCACAGACACCACAAAACCTCAGTTTTTCTccacca > 2:14255227/1‑136 (MQ=255) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGTCCGCGAAGAGGACAGAACAGACACCACAAAAACCCCATTTTTCTccccccg > 1:8033993/1‑137 (MQ=255) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACCGACACCACAAAAACTCAGTTTTTCTccgccca > 2:9722281/1‑136 (MQ=255) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGACCCTTC‑GCGGCCTTTCCCGCGAAGAGGCCGGCACAGAAACCACAAAAAATCAGTTTTTCTccgccca > 1:14993304/1‑136 (MQ=255) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGAACCTTC‑GCGGCCCTTCCCACGAAGAGGCCAGCACAAACACCACAAAAACTCAGTTTTTTTccccccc > 1:11324339/1‑136 (MQ=255) gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt < 2:16494314/137‑1 (MQ=255) tAAGCTGCAGTATCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTc < 2:16160296/137‑1 (MQ=255) aaGTTGCGGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTGTTTGTCCTGTGCCGGCCTTTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCa < 1:9863537/137‑1 (MQ=255) aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGTCCCCTCGGCGGCCTTCCCCACAAAGAGACAGGAAAAGA‑AACACAAAAACTTAATTTTTCCccacccaaccc > 2:15715229/1‑132 (MQ=255) aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GTGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCa < 1:3995186/137‑1 (MQ=255) aaGCTGCAGTAGCCGAACGAAGGAAGGCTGCCCTCGGGCAGCC‑TTTTGTATTTGTCCTGTTCCGGCCTCTTC‑TCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCa < 2:4861849/137‑1 (MQ=255) gttgaaGTGGCTGAACGAAGGAAGTTGGCCCTCGGGAAG‑CTTTTTTTGTTTGTCTTGTTCCGGCCTTTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:6068773/132‑1 (MQ=255) gttGCAGTAGCCGAACAAAAGAAGGCTGCCCTTGGGCATCC‑TTTTGTGTTTGTCCTGTTCCGGCCTTTTC‑GTGGCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:12917470/135‑1 (MQ=255) gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:17302698/137‑1 (MQ=255) gCTGCAGTAGCCGAACGAAAGAAGGCTGCCCTTGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:15291052/137‑1 (MQ=255) gCTGCAGGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CTTTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:2012600/137‑1 (MQ=255) gCTGAAGTAGCCGAACGAAAGAAGGCTGCCTTCGGGCAGCC‑TTTTTTATTTGTCTTGTGCCGGCCTCTTC‑GCGGCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg < 1:24922/137‑1 (MQ=255) cTGCAGTAGCCAAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc < 1:7529186/137‑1 (MQ=255) cTGCAGGAGCCGAAAACAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCTGGCCTCTTC‑GTGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc < 1:17739331/137‑1 (MQ=255) cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCCGCCCGCTCACGCAGc > 2:6277718/1‑137 (MQ=255) ggaGCCGAACGAAAGAGGGTTGCCCTGGGGAAG‑CTTTTTTTGTTTGTCCTGTGCTGTCCTCTTT‑GCGGCCTTTCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc < 1:438688/135‑1 (MQ=255) gTAGCCGAACGCAAGAAGGCTGCCCTTGGGCAGCC‑TTTTTTATTTGTCTTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc < 2:197051/137‑1 (MQ=255) gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc < 2:12715162/137‑1 (MQ=255) aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt < 2:10244568/137‑1 (MQ=255) aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt < 2:12618560/137‑1 (MQ=255) ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAACCCtttt > 2:989443/1‑136 (MQ=38) gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACCCCACAAAACCTCAATTTTTCTCCGCCCGCTCACGCAGCCCCTTCAg > 1:3342768/1‑137 (MQ=38) cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGt > 2:3827971/1‑137 (MQ=255) cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTTCGGACTCTTC‑GCGGCCCTTCCCGCGAAAAGGCCAGCACCAACACCACCAAAACCCAATTTTTCTCCACCCACCCCAGCACCACTTTCAAGGTAt > 1:7981274/1‑137 (MQ=255) cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGGCACCACAAAACCCCAGTTTTTTTCCGCCCGATCACGCAGCCCTTTCAGGGTAt > 1:13580027/1‑137 (MQ=38) cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt > 2:3150190/1‑137 (MQ=255) cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt > 2:2741938/1‑137 (MQ=255) cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt < 2:18449896/137‑1 (MQ=255) cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTC‑GCGGCCTTGCCCCCGAAAAGGCCGGCAAAAACACCACAAAACCTCAGTTTTTTTCCGCCCGCTCCCGCAACCCTTTTCGGGTAt > 1:9992973/1‑137 (MQ=255) ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACg > 2:16335997/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGGCTCTTC‑GCGGCCTTGCCCACGAAAAGGACGGAAAAGAAACCCCAAAACATCAGTTTTTCTCCGCCCACTCACGCAACCCCTTCCGGGTATTGAACAGTGCATcc > 1:3646079/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCCCAAAAACTCAGTTTTTCTCCCCCCGCTCACGCAGCCCTTTCAGGGTATTGAAAGGCGTATcc > 1:11193651/1‑137 (MQ=37) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCATTTCAGGGTATTGAACGGCGCATcc > 2:8807390/1‑137 (MQ=39) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCACTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc > 2:10346124/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAAAGGACGGCACAAACACCACCAAACCTCCGTTTTTCTCCGCCCACTCACGCAACCCTTTCAGGGTATTGGACGGTGCATcc > 1:6663440/1‑137 (MQ=255) tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCAAAAAGGCCGGAACAGACACCACAAAAACTCCATTTTTTTCCGCCCACTCCCGCAGCCCTTTCAGGGGATTGGAAGGCGCAccc > 1:16568207/1‑137 (MQ=255) ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc < 1:3217924/137‑1 (MQ=255) cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGACCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAACCCTTTCAGGGTATTGAACGGCGCATCcaccac > 2:10689018/1‑137 (MQ=255) ttttttATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACt > 2:3151350/1‑137 (MQ=255) tttttATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTCTTGAACGGCGCATCCACCACGAACtt > 2:13964725/1‑137 (MQ=255) tttttATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCCCCGCCCGCTCACGCAACCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt > 2:6788139/1‑137 (MQ=255) | AGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC > AE015451_phaM1/4740690‑4740949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |