Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 4,740,819 2 bp→CT intergenic (+56/+696) gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,740,8190TC95.7% 1244.8 / ‑0.4 352intergenic (+56/+697)gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)–/–
Reads supporting (aligned to +/- strand):  ref base T (1/14);  new base C (208/129);  total (209/143)
Fisher's exact test for biased strand distribution p-value = 2.17e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM14,740,8200GT99.1% 1196.1 / 2.3 352intergenic (+57/+696)gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)–/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base T (208/139);  minor base . (1/2);  total (209/143)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 353 total aligned reads displayed.

AGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC  >  AE015451_phaM1/4740690‑4740949
                                                                                                                                 ||                                                                                                                                  
aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGTTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                             >  2:6398721/1‑137 (MQ=255)
    cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                         <  1:6153036/137‑1 (MQ=255)
     ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                        <  1:1037505/137‑1 (MQ=255)
         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTctc                                                                                                                    >  1:11859260/1‑135 (MQ=255)
         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc                                                                                                                   >  2:3698335/1‑137 (MQ=255)
         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc                                                                                                                   >  2:17140632/1‑137 (MQ=255)
         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc                                                                                                                   >  2:16021307/1‑137 (MQ=255)
         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc                                                                                                                   >  2:15001985/1‑137 (MQ=255)
         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc                                                                                                                   >  2:5314656/1‑137 (MQ=255)
         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc‑gc                                                                                                                   >  1:14673659/1‑137 (MQ=255)
         gTCCGCGCCAGCTGTACACCAGCGAGCAGACGCGCGACATCGTTGCCCTGAAAGACCGCACGTAAGCTGCAGTAGCCGAACGCACGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGTCTCTTc‑gc                                                                                                                   >  1:8734779/1‑137 (MQ=255)
               gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCtt                                                                                                             <  2:13895967/137‑1 (MQ=255)
                ccAGTTGTACACCGGCGAGAAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGGAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTGTTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTg                                                                                                            <  1:6300109/137‑1 (MQ=255)
                 cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTC‑GCGGGCTTGc                                                                                                           >  2:1199626/1‑137 (MQ=255)
                     tGTACACTGGCGGGCAGAAGCGTGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTACAGTAGCCGAATGCAAGAAGGCTGCCCTGGGGCAG‑CTTTTTTTATTTGTCCTGTGCTGGCCTTTTC‑GAGGCCTTGCCcgc                                                                                                       <  2:2214204/137‑1 (MQ=255)
                     tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCGGGAGCCGAACGCAGGAGGGCTGCCCTCGGGCAG‑CTTTTTTTGTTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCcgc                                                                                                       <  2:11139391/137‑1 (MQ=255)
                         cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCTCAAGTAGGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAag                                                                                                   <  2:11764633/137‑1 (MQ=255)
                         cacCGGAAAACAAAAGCACAACACCGTTCCCTGGAAAGACCGCAAGAAAGCGGAAGAAGCAGAACGAAAGAAGGCTCCCCTCGGGCAGCC‑TTTTTTATTTTTCTTGTTCCGGCCCCTTC‑GCGACTTTCCCCGCAAag                                                                                                   <  2:574992/137‑1 (MQ=255)
                           ccGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAATGCTTCCCTCTGGCAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAagag                                                                                                 <  2:18161366/137‑1 (MQ=255)
                                 aaaaGAAGCGCAACATCGTTGCCCGGAAAGACCGCGAGTAGACTGCAGTAGCTGAACGCAAGAAGACTGCCCTTTGGCAG‑CTTGTTTGAGTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCTCGAGAAGAGGCCGGc                                                                                           <  1:5439268/134‑1 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCGGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:9433536/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGNCCTCGGGCAGCC‑TTTTTTACTTGTCCTGTGCCGGCCCCTTC‑GCAGCCTTGCCCACGAAGAggacaga                                                                                           >  1:16063972/1‑132 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑TCCGCCTTTCCCACGAAAAAGACGGc                                                                                           >  1:144932/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCTGTCCTTCCCACGAAGAGGcaaga                                                                                           >  1:1758725/1‑133 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGTCTTGCCCGCGAAGAGGCCggt                                                                                           >  1:18139433/1‑136 (MQ=38)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGGGGCCGGc                                                                                           >  2:5743630/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGGGGCCGGc                                                                                           >  1:9326775/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAgggcagc                                                                                           >  1:18049113/1‑132 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:9063054/1‑137 (MQ=39)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  1:12255686/1‑137 (MQ=39)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:2543638/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:17524121/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:13106257/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:14633338/1‑137 (MQ=39)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:15399210/1‑137 (MQ=39)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAAGccaga                                                                                           >  1:2795325/1‑134 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAAAGGCCGGc                                                                                           >  1:7882711/1‑137 (MQ=38)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCAAAGAGGCCGGc                                                                                           >  1:2640521/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTC‑GCGGCCTTTTCCGCGAAGAGGACGGc                                                                                           >  1:1000776/1‑137 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTC‑GCGGCCTTTCCCTCGAAAAggactgc                                                                                           >  2:4683165/1‑132 (MQ=255)
                                 aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGACTCTTC‑GCGTTCTTTTCCTCGCAGAgggaggg                                                                                           >  1:14172999/1‑132 (MQ=255)
                                 aGCAGAAGCGCGACACCGTTGCCCTGAAAGACCGCAAGTAAACTGCAGTAGCAGAACACAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:12474676/1‑137 (MQ=38)
                                    aGAAGCGCGACATCGTTGCCCTGAAAGACCGCCAGTAAGCTGCAGTAGCCGAACGCAAGAGGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCTGCGAAGAGTCCGGcaca                                                                                        >  2:17352912/1‑137 (MQ=255)
                                    aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGccca                                                                                        >  1:13807086/1‑137 (MQ=255)
                                    aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGcaca                                                                                        >  1:18621798/1‑137 (MQ=39)
                                    aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCACGAAAAGGCCGGcaca                                                                                        >  1:17062940/1‑137 (MQ=255)
                                    aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCACGAAAAGGACGGaaca                                                                                        >  1:9417507/1‑137 (MQ=255)
                                                tCGTTGCCCTGAAAGACTGCAAGTAAGCTGCAGTGGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTACCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                            <  2:5158430/137‑1 (MQ=255)
                                                             aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct                                                               <  2:5643664/137‑1 (MQ=255)
                                                              gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                              >  2:131433/1‑137 (MQ=255)
                                                              gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTC‑GCGGCCTTGCCCGCGAAAAGGCCGGCACAAACACCCCAAAACCCCCATTTTtctc                                                              >  1:10845093/1‑137 (MQ=37)
                                                               aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                             <  2:8195338/137‑1 (MQ=255)
                                                                 cacAAGTAAGCTGCAGGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCGGTGCCGGCCTCTTT‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                           <  1:9114565/135‑1 (MQ=255)
                                                                   cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGTC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCTGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                         >  2:18414651/1‑137 (MQ=255)
                                                                   cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGACCTCTTC‑GCAGACTTGCCCGCGAAGAGGCCAGCACAGACACCACAAAACCTCAGTTTTTCTccacca                                                         >  2:14255227/1‑136 (MQ=255)
                                                                    aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGTCCGCGAAGAGGACAGAACAGACACCACAAAAACCCCATTTTTCTccccccg                                                        >  1:8033993/1‑137 (MQ=255)
                                                                    aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACCGACACCACAAAAACTCAGTTTTTCTccgccca                                                        >  2:9722281/1‑136 (MQ=255)
                                                                    aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGACCCTTC‑GCGGCCTTTCCCGCGAAGAGGCCGGCACAGAAACCACAAAAAATCAGTTTTTCTccgccca                                                        >  1:14993304/1‑136 (MQ=255)
                                                                    aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGAACCTTC‑GCGGCCCTTCCCACGAAGAGGCCAGCACAAACACCACAAAAACTCAGTTTTTTTccccccc                                                        >  1:11324339/1‑136 (MQ=255)
                                                                      gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                      <  2:16494314/137‑1 (MQ=255)
                                                                       tAAGCTGCAGTATCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTc                                                     <  2:16160296/137‑1 (MQ=255)
                                                                        aaGTTGCGGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTGTTTGTCCTGTGCCGGCCTTTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCa                                                    <  1:9863537/137‑1 (MQ=255)
                                                                        aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGTCCCCTCGGCGGCCTTCCCCACAAAGAGACAGGAAAAGA‑AACACAAAAACTTAATTTTTCCccacccaaccc                                                    >  2:15715229/1‑132 (MQ=255)
                                                                        aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GTGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCa                                                    <  1:3995186/137‑1 (MQ=255)
                                                                        aaGCTGCAGTAGCCGAACGAAGGAAGGCTGCCCTCGGGCAGCC‑TTTTGTATTTGTCCTGTTCCGGCCTCTTC‑TCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCa                                                    <  2:4861849/137‑1 (MQ=255)
                                                                          gttgaaGTGGCTGAACGAAGGAAGTTGGCCCTCGGGAAG‑CTTTTTTTGTTTGTCTTGTTCCGGCCTTTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                  <  1:6068773/132‑1 (MQ=255)
                                                                          gttGCAGTAGCCGAACAAAAGAAGGCTGCCCTTGGGCATCC‑TTTTGTGTTTGTCCTGTTCCGGCCTTTTC‑GTGGCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                  <  1:12917470/135‑1 (MQ=255)
                                                                          gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                  <  1:17302698/137‑1 (MQ=255)
                                                                          gCTGCAGTAGCCGAACGAAAGAAGGCTGCCCTTGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                  <  1:15291052/137‑1 (MQ=255)
                                                                          gCTGCAGGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CTTTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                  <  1:2012600/137‑1 (MQ=255)
                                                                          gCTGAAGTAGCCGAACGAAAGAAGGCTGCCTTCGGGCAGCC‑TTTTTTATTTGTCTTGTGCCGGCCTCTTC‑GCGGCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                  <  1:24922/137‑1 (MQ=255)
                                                                           cTGCAGTAGCCAAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc                                                 <  1:7529186/137‑1 (MQ=255)
                                                                           cTGCAGGAGCCGAAAACAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCTGGCCTCTTC‑GTGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc                                                 <  1:17739331/137‑1 (MQ=255)
                                                                              cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCCGCCCGCTCACGCAGc                                              >  2:6277718/1‑137 (MQ=255)
                                                                                ggaGCCGAACGAAAGAGGGTTGCCCTGGGGAAG‑CTTTTTTTGTTTGTCCTGTGCTGTCCTCTTT‑GCGGCCTTTCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                            <  1:438688/135‑1 (MQ=255)
                                                                                gTAGCCGAACGCAAGAAGGCTGCCCTTGGGCAGCC‑TTTTTTATTTGTCTTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                            <  2:197051/137‑1 (MQ=255)
                                                                                gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                            <  2:12715162/137‑1 (MQ=255)
                                                                                  aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  2:10244568/137‑1 (MQ=255)
                                                                                  aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  2:12618560/137‑1 (MQ=255)
                                                                                    ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAACCCtttt                                        >  2:989443/1‑136 (MQ=38)
                                                                                      gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACCCCACAAAACCTCAATTTTTCTCCGCCCGCTCACGCAGCCCCTTCAg                                      >  1:3342768/1‑137 (MQ=38)
                                                                                         cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGt                                   >  2:3827971/1‑137 (MQ=255)
                                                                                           cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTTCGGACTCTTC‑GCGGCCCTTCCCGCGAAAAGGCCAGCACCAACACCACCAAAACCCAATTTTTCTCCACCCACCCCAGCACCACTTTCAAGGTAt                                 >  1:7981274/1‑137 (MQ=255)
                                                                                           cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGGCACCACAAAACCCCAGTTTTTTTCCGCCCGATCACGCAGCCCTTTCAGGGTAt                                 >  1:13580027/1‑137 (MQ=38)
                                                                                           cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                 >  2:3150190/1‑137 (MQ=255)
                                                                                           cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                 >  2:2741938/1‑137 (MQ=255)
                                                                                           cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                 <  2:18449896/137‑1 (MQ=255)
                                                                                           cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTC‑GCGGCCTTGCCCCCGAAAAGGCCGGCAAAAACACCACAAAACCTCAGTTTTTTTCCGCCCGCTCCCGCAACCCTTTTCGGGTAt                                 >  1:9992973/1‑137 (MQ=255)
                                                                                                 ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACg                           >  2:16335997/1‑137 (MQ=255)
                                                                                                         tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGGCTCTTC‑GCGGCCTTGCCCACGAAAAGGACGGAAAAGAAACCCCAAAACATCAGTTTTTCTCCGCCCACTCACGCAACCCCTTCCGGGTATTGAACAGTGCATcc                   >  1:3646079/1‑137 (MQ=255)
                                                                                                         tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCCCAAAAACTCAGTTTTTCTCCCCCCGCTCACGCAGCCCTTTCAGGGTATTGAAAGGCGTATcc                   >  1:11193651/1‑137 (MQ=37)
                                                                                                         tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCATTTCAGGGTATTGAACGGCGCATcc                   >  2:8807390/1‑137 (MQ=39)
                                                                                                         tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCACTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc                   >  2:10346124/1‑137 (MQ=255)
                                                                                                         tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAAAGGACGGCACAAACACCACCAAACCTCCGTTTTTCTCCGCCCACTCACGCAACCCTTTCAGGGTATTGGACGGTGCATcc                   >  1:6663440/1‑137 (MQ=255)
                                                                                                         tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCAAAAAGGCCGGAACAGACACCACAAAAACTCCATTTTTTTCCGCCCACTCCCGCAGCCCTTTCAGGGGATTGGAAGGCGCAccc                   >  1:16568207/1‑137 (MQ=255)
                                                                                                            ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc                <  1:3217924/137‑1 (MQ=255)
                                                                                                              cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGACCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAACCCTTTCAGGGTATTGAACGGCGCATCcaccac              >  2:10689018/1‑137 (MQ=255)
                                                                                                                    ttttttATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACt         >  2:3151350/1‑137 (MQ=255)
                                                                                                                     tttttATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTCTTGAACGGCGCATCCACCACGAACtt        >  2:13964725/1‑137 (MQ=255)
                                                                                                                     tttttATTTGTCCTGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCCCCGCCCGCTCACGCAACCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt        >  2:6788139/1‑137 (MQ=255)
                                                                                                                                 ||                                                                                                                                  
AGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTC‑GCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC  >  AE015451_phaM1/4740690‑4740949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑