| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,932,640 | +C | intergenic (‑107/+43) | rluA CDS (23S rRNA (746) and tRNA (32) pseudouridine synthase) ← / ← minE CDS (Cell division topological specificity factor) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,932,638 | 1 | . | C | 91.0% | 1654.4 / 164.3 | 486 | intergenic (‑105/+45) | rluA CDS (23S rRNA (746) and tRNA (32) pseudouridine synthase)/minE CDS (Cell division topological specificity factor) | –/– |
| Reads supporting (aligned to +/- strand): ref base . (37/6); new base C (243/191); total (289/197) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.45e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 509 total aligned reads displayed.
TCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAT > AE015451_phaM1/1932502‑1932769 | tCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGTAAGCCCGCTCCCACAAAGGAGATGGGTGCCCTGTGGGAGG‑GGG‑G‑T‑Tg > 2:1852570/1‑137 (MQ=255)tCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAAGATATGCGTCCCCCGTAGGAGC‑GGG‑C‑T‑Tg > 2:9150058/1‑137 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TG‑ccc < 2:19237998/137‑2 (MQ=255) agGATTTGGACGTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGC‑GGGCAAGCCCGCTCCTACAAGGGATATGCGTCCCCTGGAGGAGC‑GGG‑C‑T‑TG‑cccgcg < 2:7673810/137‑5 (MQ=38) cTGGACATTCGACAGCGGCGTTGCGGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTTGC‑GTGCAAGCCCGCTCCTACAAATAATATGCTTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAggg < 2:12287635/137‑1 (MQ=37) cTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAAACCACTCCCCCCAAAGAGACGCGTCCCCCCTAGGGGC‑GGGAT‑T‑TT‑CCCCGAAggg > 1:9986674/1‑137 (MQ=255) cgttCGACAGCGACGTTGCAGGTCTCACGAAAAGAGAAGGAAACGTGGGGGCCGCTTTGTGGCCCTTCGT‑GGACAAGCCCGCTCCTACAAAGGATGTGTGTCCCTTGGAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCa < 1:1714319/135‑1 (MQ=21) cATTCGACAGCGACATTGCAGTTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTTCGACCCTTCGC‑GGGCAAACCCGCTCCTACAAAGGATAGGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCa < 1:14014172/137‑1 (MQ=37) tCGAAAGCGGAATTGCAGGTCTAACGAAAAGAGCAGGAAACGCTGGGGCCGCTTTGTGGCCTTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGGAGGAGC‑GGG‑T‑T‑TGCCCGCGAAGGGCTGCAAgg < 1:13494962/137‑1 (MQ=37) gACAGTTGCATGACAGGTCTCACGAAAAGAGAAGTAAACGCTTGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAACCCTCCCCTACAAAGGATATGTTCCCCCGGGGGGAGC‑GGG‑T‑T‑TGCCCGCGAAGGGCTGCAAGGCa < 1:18331014/137‑1 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTCCAAAAGACATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCAAAGGGCTGCAAGGCAg > 2:6413741/1‑137 (MQ=38) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCACTCCTACAAAGGATACGCGTCCCCTGTAGGAGC‑GGG‑CTT‑TC‑CCGCGAAGGGCTGCAAGGCAg > 2:6244983/1‑137 (MQ=38) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAACCCGCTCCTACAAAAGATATGCGTCCCCCGTAGGAGC‑GGG‑C‑T‑TGTCCGCGAAGGGCTGCAAGGCAg > 2:15882728/1‑137 (MQ=38) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAAACCACTCCTACAAAGGAGATGCGTCCCCCGTCGGAGCAGGA‑T‑T‑TC‑CCACAAAGGGATGCaaagaag > 1:3109192/1‑132 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGA‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGGGA‑GTG‑C‑T‑TGCCCACGAAGGGATGTaaaggag > 2:10667527/1‑132 (MQ=255) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCTTCCCTTGTGGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGc < 1:17950570/137‑1 (MQ=38) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGc < 1:10477342/137‑1 (MQ=255) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTCCCAAGGATATGCGTCCCCTGTCGGAAC‑GGG‑C‑T‑TGCCCGCAAAGGGCTGCAAAGCAGCCCCa > 2:4464273/1‑137 (MQ=37) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGAATATGCGTCCCCTGTAGGAGC‑GGGTT‑T‑TC‑CCGCGAAGGGCTGCAAAGCAGCCCCa > 2:13039729/1‑137 (MQ=37) aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGGAAAACCCGCCCCCCCAAAGGAAATGTTTCCCCTGTAGGGAG‑GGG‑T‑T‑TGCCCCCAAAGGGCTGCAAGGAAACCCCaaa > 1:15428957/1‑137 (MQ=255) ttGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCTTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt < 2:19482830/137‑1 (MQ=255) tggcgggTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGCCCCTTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt < 1:2906219/132‑1 (MQ=37) tGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTCCAAAAGAAATGTGTCCCCCCTAGGAGG‑GGGAT‑T‑TC‑CCGCGAAAGGATGTAAAGGAAACCCaaactt > 1:11409683/1‑134 (MQ=19) ttctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAc < 2:3146734/136‑1 (MQ=255) tctcACGAAAAGAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt < 2:5291932/137‑1 (MQ=255) tctcACAAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCATCCCCTTTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt < 2:17124435/137‑1 (MQ=38) cctcACGAAAAGAGAAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGCCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt < 1:1566496/136‑1 (MQ=37) aaaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGAAAGCCCGCCCCTCCAAAGGGTGTGCGTCCCCCGTAGGAGC‑GGG‑C‑C‑TGCCCCCGAAGGGATGCAAGGCAACCCCAAACTGTCAACTCCGCGGa > 2:5617696/1‑137 (MQ=255) aTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 2:1131992/137‑1 (MQ=255) gaGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 2:14363623/136‑1 (MQ=255) gCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGGAAGACCACTCCCCCAAAAGAGATGCGTCCCCCTTGGGAGG‑GGG‑C‑T‑TGTCCGCGAAAGGCTGCCAAGCAGCCCCAAACTTTCTACTCCACGGATCAGCg > 1:8808366/1‑137 (MQ=255) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGT‑GGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg < 1:19102731/137‑1 (MQ=255) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAGGCCCATTCCGAAAAAGGAGATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg < 2:6137481/137‑1 (MQ=38) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg < 2:8431669/137‑1 (MQ=255) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg < 2:10764593/137‑1 (MQ=255) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGACAAGCCCGCTCCTACAAAGGATATGCTTCCCCTGGAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg < 1:4427868/137‑1 (MQ=39) gCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGAAAGCCCGCTCCTCCAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCACGAAGGGCTGCAAAGCCGCCCCAAATTGTCGACCACGCGGATCCGCGATCCGGAAg > 2:12846924/1‑137 (MQ=255) ttGGGGCCGTTTTGCGGCCCTTCGC‑GGGCAAGCCCTCTCCTACAAAGAATATGCTTCCCTTGTAGGAGC‑GGG‑C‑T‑TTCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 1:14302241/136‑1 (MQ=37) cgggggCCGCTTTGTGGCCTTTCGC‑GGGAAAGCCCGCTCCTAAAAAGGATATACGTCCCCTGGGGGAGC‑GGG‑C‑T‑GGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 1:5202006/135‑1 (MQ=37) tccGCTTTGCGGCCCTTCGC‑GGGCAACCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt < 1:14629697/136‑1 (MQ=255) ccGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGGAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 2:11046419/137‑1 (MQ=255) ttGCGGCCCTTCGC‑GGGCAAGCCCGCCCCCCCAAAGGAGATGCGTCCCCCGTAGGGGC‑GGG‑C‑T‑TGTCCGCGAAAGGCTGTCAGGCAGACCCCAACTTTCTACTCCGCGGATCCACGATCCCGCAGGGTGAtttttct > 2:10506644/1‑134 (MQ=255) gctcTTCGT‑GGGCAAGCCCGCTCCTACAAATGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa < 2:7930441/134‑1 (MQ=38) cTTCGC‑GGGCAATCCCGCTCCTACAAAGGATATGCTTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGaa < 2:6985332/137‑1 (MQ=255) cTTCGC‑GGACAAGCCCGCCCCTACAAGGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑TGTC‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGATCCAGaa < 1:8149329/137‑1 (MQ=255) cgc‑gGGCAAGCCCGCTCCTACAAAGGAGATGCTTCCCCTGTAGGGGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCg < 1:3826909/137‑1 (MQ=39) aacccGCTCCCCCAAAGGATACGCGTCCCCCGTCGGAGC‑GGG‑C‑T‑TTCCCGCGAAGGGCTGCAAGGAAGCCCCCAATTGTCGACCACACGGATCAGCGGTCCGGCAGGGTGATGTTCAGCTCCCGAATCGAGCAGCTg > 2:15331489/3‑137 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAATACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGACTCAAGCAACTGcc > 2:15081077/1‑137 (MQ=38) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt > 2:17944292/1‑137 (MQ=255) ccTACAAAGGATATGCTTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt < 1:15309784/137‑1 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt > 2:1673910/1‑137 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt > 2:12647422/1‑137 (MQ=255) cAAAGGATATGCGTCCCCTTTATGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTAGTTTTcc < 2:481890/137‑1 (MQ=39) cAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 2:225051/137‑1 (MQ=255) aaaGGATATGCTTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 2:11899851/137‑1 (MQ=255) aaaGGATATGAGTCCCCTTTAGAAGC‑GGG‑C‑T‑GGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 1:16175014/137‑1 (MQ=255) aaGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 2:3874218/1‑137 (MQ=255) aGGATATGCTTCCCCTGGAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc < 1:7452253/137‑1 (MQ=255) atatGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCg < 1:10842224/137‑1 (MQ=255) atGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat < 1:10151353/137‑1 (MQ=255) atGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat > 1:2442319/1‑137 (MQ=255) atGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGATGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat > 1:3596366/1‑137 (MQ=255) atGCGTCCCATGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat > 2:18935738/1‑137 (MQ=255) atGCGTCCACTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat > 1:7995337/1‑137 (MQ=255) tGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 2:9551541/1‑137 (MQ=255) gCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGCGCAGCTGCCCTGGTTTTCCAGCTCGatat > 2:3655208/1‑137 (MQ=255) gCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 2:13705859/1‑137 (MQ=255) gCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 2:8907689/1‑137 (MQ=255) gCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 1:18582091/1‑137 (MQ=255) gCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 2:3535777/1‑137 (MQ=255) gCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 1:8390550/1‑137 (MQ=255) gCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCAAGCTCGatat > 1:12939071/1‑137 (MQ=255) gCGTCCCATGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 2:4153812/1‑137 (MQ=255) gcgtcccTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg > 2:19284817/5‑137 (MQ=255) tCCTCTGGAGGAGC‑GGG‑C‑T‑TGCCCGAGAAGGGCTGCAAGGCAGCCCCAAATTGTCTACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCGCCAGAATCGAGCAGCTGCCCTGGGTTTCCGGCTCGATGTGca < 1:8619821/137‑1 (MQ=38) ccTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcat < 1:15095237/137‑1 (MQ=255) cTGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatc > 2:14468646/1‑137 (MQ=255) tGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCCGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 2:7424898/1‑137 (MQ=255) tGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 2:9754213/1‑137 (MQ=255) tGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 1:6665650/1‑137 (MQ=255) tGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 1:12350187/1‑137 (MQ=255) tGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 1:16704571/1‑137 (MQ=255) tGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 1:2607995/1‑137 (MQ=255) tGTAGGAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 2:17756846/1‑137 (MQ=255) gAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 1:11806771/137‑1 (MQ=255) gAGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 2:16292054/1‑137 (MQ=255) aGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTTCAGCTCGATATGCACATCATCGTTg < 1:13356337/137‑1 (MQ=255) aGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 1:17358/1‑137 (MQ=255) aGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg < 2:13287887/137‑1 (MQ=255) aGC‑GGG‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg < 2:15519300/137‑1 (MQ=255) ggg‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCg > 1:9295378/1‑137 (MQ=255) gg‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 2:6800434/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 2:7186028/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 1:19441078/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 2:2630665/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 2:582150/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 2:10205650/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 1:7191726/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 2:9264552/1‑137 (MQ=255) g‑C‑T‑TGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt < 1:5831485/137‑1 (MQ=255) | TCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGG‑C‑T‑TG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAT > AE015451_phaM1/1932502‑1932769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |