BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	4,741,228	+C	intergenic (+465/+288)	gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,741,228	1	.	C	76.2%	369.1 / 128.6	122	intergenic (+465/+288)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base . (14/15); new base C (35/58); total (49/73)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CC‑TCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACT  >  AE015451_phaM1/4741092‑4741346

tcttctTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgag                                                                                                                               <  1:10718631/137‑1 (MQ=255)

tcttctTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgag                                                                                                                               <  1:17254087/137‑1 (MQ=255)

  ttcttTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgagca                                                                                                                             >  2:5921223/1‑135 (MQ=255)

  ttcttTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgagca                                                                                                                             >  2:1205024/1‑135 (MQ=255)

  ttcttTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgagca                                                                                                                             >  1:5302617/1‑135 (MQ=255)

   tcttTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgagcag                                                                                                                            >  2:3980476/1‑134 (MQ=255)

   tcttTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgagcag                                                                                                                            >  2:11312995/1‑134 (MQ=255)

                                                                                               gAACTGGGCATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                          <  2:3547715/137‑1 (MQ=255)

                                                                                                aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                         >  1:6225339/1‑137 (MQ=255)

                                                                                                aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                         <  2:16164301/137‑1 (MQ=255)

                                                                                                aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                         >  2:7806416/1‑137 (MQ=255)

                                                                                                aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TTGTATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCCGCGCCTCGACCTTGGCCAGCGGCGCCTTGATTACGGt                         >  2:13414141/1‑137 (MQ=255)

                                                                                                aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                         >  2:16252652/1‑137 (MQ=255)

                                                                                                 aCTGGGTATAGTTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:4571809/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  2:33699/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        >  2:2338429/1‑137 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:14089077/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:19425602/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:6789681/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:6789680/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  2:1768784/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:291399/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:15997633/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:9461351/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  1:11906966/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TTGGATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  2:16563674/137‑1 (MQ=255)

                                                                                                 aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                        <  2:3018593/137‑1 (MQ=255)

                                                                                                  cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                       <  1:19214487/137‑1 (MQ=255)

                                                                                                  cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                       <  1:10793616/137‑1 (MQ=255)

                                                                                                   tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcac                      <  1:9727114/137‑1 (MQ=255)

                                                                                                     ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                    >  2:18331830/1‑137 (MQ=255)

                                                                                                     ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                    >  2:259427/1‑137 (MQ=255)

                                                                                                     ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                    >  2:8510145/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTTACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  1:2764689/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  2:15063992/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:1483769/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:16054880/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:126484/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:126439/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  2:18356687/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  1:10295318/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  2:2186268/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:8200929/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  1:15628278/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  1:2891790/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  1:3301969/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  1:17532466/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  1:13549641/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  1:17174621/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  1:14808308/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  1:1204211/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  1:16738497/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  1:16312819/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  1:16312774/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  1:1519012/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  2:11392966/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCCTGATCACGGTCACACcgc                 <  1:8056382/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:13670318/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:15390722/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACATGCCCCGGACACGTCCTCCTGCAGCGCCTCTACCTCGGCCAGCTGCGCCTTGATCACGGTCACACcgc                 <  1:14361532/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 >  2:17767417/1‑137 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  2:17644378/137‑1 (MQ=255)

                                                                                                        ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCGTGATCACGGTCACACcgc                 <  2:7008810/137‑1 (MQ=255)

                                                                                                        ataGGTCCAACTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TTGCATTCTTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                 <  2:13712611/137‑1 (MQ=255)

                                                                                                          aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa               >  2:11992392/1‑137 (MQ=255)

                                                                                                             tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg            >  2:4764793/1‑137 (MQ=255)

                                                                                                             tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg            <  2:15512575/137‑1 (MQ=255)

                                                                                                             tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg            >  1:11317972/1‑137 (MQ=255)

                                                                                                             tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg            >  2:9121196/1‑137 (MQ=255)

                                                                                                             tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg            <  2:1005384/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  1:13588078/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  2:14878032/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  2:13541830/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  1:5074026/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  2:7555771/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  1:7583902/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  1:9606283/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg           <  1:9952488/137‑1 (MQ=255)

                                                                                                              ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGTTCACACCGCGATAgg           <  2:9185893/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  1:4637568/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  1:9361081/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  2:9130168/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  1:11545637/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  2:13351585/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  2:6279429/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  2:2648906/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          >  2:13149904/1‑137 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  1:5849832/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGATCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  2:18394297/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  2:17497824/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTCTGCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          <  2:14354569/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATATGc          <  2:14354568/137‑1 (MQ=255)

                                                                                                               cAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc          >  2:3385679/1‑137 (MQ=255)

                                                                                                                aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc         >  1:14235918/1‑137 (MQ=255)

                                                                                                                aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc         >  1:14235919/1‑137 (MQ=255)

                                                                                                                  ctctGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt       >  2:15677166/1‑137 (MQ=255)

                                                                                                                  ctctGGTCACCTTCGTGCTTGAGCAG‑CT‑TCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCAGGACACGTCCTCCTGCAGCGCCTCGAACTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt       >  2:13166502/1‑137 (MQ=255)

                                                                                                                  ctctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGAACTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCAtt       >  2:19347983/1‑137 (MQ=255)

                                                                                                                  ctctGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt       >  2:4313681/1‑137 (MQ=255)

                                                                                                                  ctctGGTCACCTTCGTGCTTGAG‑CA‑GC‑TTC‑TGCGAC‑TNGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt       >  1:18825974/1‑137 (MQ=255)

TCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CC‑TCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACT  >  AE015451_phaM1/4741092‑4741346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`