Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,344,3080GA42.0% 100.8 / 34.1 141intergenic (+39/‑117)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
Reads supporting (aligned to +/- strand):  ref base G (50/26);  new base A (54/1);  total (114/27)
Fisher's exact test for biased strand distribution p-value = 1.50e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 190 total aligned reads displayed.

GGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTT  >  AE015451_phaM1/4344173‑4344444
                                                                                                                                        |                                                                                                                                        
gggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgc                                                                                                                                         <  1:16862567/137‑1 (MQ=255)
 ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTTCCCGATTCAGCaccacc                                                                                                                                        >  2:7946818/1‑137 (MQ=255)
 ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGCCAAAGCTAGCaccacc                                                                                                                                        >  1:16164651/1‑137 (MQ=255)
 ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAAATCGAACaccacc                                                                                                                                        >  2:10106310/1‑137 (MQ=255)
 ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGAACGAGAaccacc                                                                                                                                        >  2:1971358/1‑137 (MQ=255)
 ggCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAATCGAGCAccgcc                                                                                                                                        >  2:4371932/1‑137 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCGTTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:12698062/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGTGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CACGCCAGATCAAGGAccgccc                                                                                                                                       >  2:13055273/1‑137 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTTCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:3770241/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCT‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:17174456/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:18723651/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:15593784/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:8536326/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:11931298/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:718439/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:10943596/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:4710594/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  2:3767065/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:12992683/137‑1 (MQ=255)
  gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCAGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAcagccc                                                                                                                                       >  2:13055272/1‑137 (MQ=255)
   cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgcccg                                                                                                                                      <  1:4448396/137‑1 (MQ=255)
   cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgcccg                                                                                                                                      <  2:10052884/137‑1 (MQ=255)
    gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgcacgc                                                                                                                                     >  2:14397709/1‑137 (MQ=255)
     aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGTG‑CCTCCCAGATCGAGCACCGCCcacg                                                                                                                                    >  2:13328374/1‑137 (MQ=255)
      cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAGATCGAGCACCGCCcgcgc                                                                                                                                   >  2:18715821/1‑137 (MQ=255)
       aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  2:19308555/137‑1 (MQ=255)
       aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  1:6339241/137‑1 (MQ=255)
       aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  2:14596591/137‑1 (MQ=255)
       aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  1:8022508/137‑1 (MQ=255)
       aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                  <  2:4879300/137‑1 (MQ=255)
        gCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCgg                                                                                                                                 <  1:15266618/137‑1 (MQ=255)
           tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCCAATCGAGCCCCACCCCCACAGcac                                                                                                                              >  1:7369891/1‑137 (MQ=255)
           tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                              <  1:10217772/137‑1 (MQ=255)
           tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                              <  2:18152358/137‑1 (MQ=255)
           tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGACCCAACACCACCCCCGCGGccc                                                                                                                              >  1:8878601/1‑135 (MQ=255)
             aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                            <  1:12266207/137‑1 (MQ=255)
              aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTTCCCGATCGAGAACCGCCCACCCGGCccaca                                                                                                                           >  1:16178156/1‑136 (MQ=255)
              aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCACATCGCGAACCACACACACGGAacaca                                                                                                                           >  1:7737169/1‑136 (MQ=255)
              aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTGCCAGATCTAACACCCCCCACGCTGTacaca                                                                                                                           >  1:2255287/1‑136 (MQ=255)
                  gCGTTGGGCAGTGGAAGACTCGTGCGTTTATACAGAAGGGGCGGAGAGCGACCGGAGCAATTCGGCTGGGGCGCGTTAACTCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGATCCCCGCCCGCGCGGCACACGATCt                                                                                                                       <  2:17560142/137‑1 (MQ=255)
                      tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCa                                                                                                                   >  2:13676142/1‑137 (MQ=255)
                      tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAACCGAGCACCGCCCACGCGGAACACGATCTGGCa                                                                                                                   >  2:16574424/1‑137 (MQ=255)
                      tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAGATCGAGCACCGCCCACGCGGCACACAACCTggaa                                                                                                                   >  2:4177434/1‑135 (MQ=255)
                      tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAAACCTAGCACCCCCCACACGGCACACAAACTGGCa                                                                                                                   >  2:15832203/1‑137 (MQ=255)
                       gggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCTTCCGATCGAGCACCACCCACACAGCACACCAACTggtaa                                                                                                                  >  1:5678225/1‑134 (MQ=255)
                        ggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGTG‑GCTGTTAAGTTGAGGACCACCCGCGCAGGGCAAAATCTTGCAgg                                                                                                                 >  1:11552614/1‑137 (MQ=255)
                           aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAAGCTAACACCACCCACACGACACACAAACTGGCAggtaa                                                                                                              >  1:12063917/1‑134 (MQ=255)
                           aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAGACCGAGCACCGCCCGCGCGGCACACGATCTGGAAGgcgt                                                                                                              >  2:17777035/1‑136 (MQ=255)
                             tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCCGATCGAGCCCCCCCCACACGGAACAAAATCTGGCAAGCGCTg                                                                                                            >  2:3646741/1‑137 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGTG‑GCTGCCCGATCGAGAACCACCCACGCGGGACACGATCTTGCAGGAACTGCaa                                                                                                         >  1:3328152/1‑136 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCAAATCGAACACCACCCACCCAGCACAAAAGCTGGCAAGGGATGCaa                                                                                                         >  1:9511904/1‑136 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGCCCGATTGCGCACCACCCGCGCGGCACACAAGCTGGCAGGCGATGCAc                                                                                                         >  2:6193502/1‑137 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCCGATCGCGCACCACCCGCGCGGCACACAATCTTGCAAGAGATGCAc                                                                                                         >  2:15476134/1‑137 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGTCAGACCGAGCACCACCCGCGCGGAACACTATCTGGCAGGCGCTGCaa                                                                                                         >  2:14907057/1‑136 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       <  1:18544173/107‑1 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgccc                                                                                                                                       >  2:18544173/1‑107 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCGACCGAACACCACCCGCGCGGCACACGACCTGGCAGGAGATGCAc                                                                                                         >  1:18072089/1‑137 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAATCGAGCACCACCCGCACGGCACACAATCTTGCAAGCGCttccc                                                                                                         >  2:12314382/1‑133 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAATCGAACACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGccc                                                                                                         >  1:18754832/1‑135 (MQ=255)
                                cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTTCCCGACCTAGCACCACCCACGCGGCACACGATCTGGCAAGAGATGCaa                                                                                                         >  1:7623320/1‑136 (MQ=255)
                                     gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGACCGAGCACCACCCACGCAGAACACGACCCGGCAGGAGCCGGCCTAttt                                                                                                    >  2:16417501/1‑136 (MQ=255)
                                     gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTGCCCGATCAAGGACCGCCCCCGCAGCACACAACTTGGCAGGAGCCGCaacactg                                                                                                    >  1:14060210/1‑131 (MQ=255)
                                          cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTTCCAACCGAGCACCGCCCACGCGGAACACGATCTGGTGGGCGCTGTACTATTGGAggg                                                                                               >  1:8745101/1‑136 (MQ=255)
                                          cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTACCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                               <  1:19179795/137‑1 (MQ=255)
                                            tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCGAGCGAGTCCCACCCACGTGGGACAAGACCTGGTCGGCGATTCACCACTTGAGGCGt                                                                                             >  2:7192631/1‑137 (MQ=255)
                                                  ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTGGCAGAGCGAACACCACCCCCACAGCAAACAAACCGGCAGGCACCTCACCACTTGAGGCGTACAccc                                                                                       >  1:9797564/1‑137 (MQ=255)
                                                    gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGAGTCCGCCCCg                                                                                     >  2:4243664/1‑137 (MQ=255)
                                                      aTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCAATCGAGCACCACCCACGCGGAACCCAATCTTGCAAGTGCTTCAATCTTTGAGGTGTACACCCCCTc                                                                                   >  2:17218553/1‑137 (MQ=255)
                                                       tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCCGATCGAACACCACCCACGCAGAACACAAGCTGGCAGGAGTTTGACTCTTTGAGGAGTGCGCCCCTTcc                                                                                  >  1:12582197/1‑136 (MQ=255)
                                                       tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAATCCAGCACCCCCCACACGGCACACAATCTGGCAGGAGCGGCACCATTGGAGGAGGACGCACCATca                                                                                  >  1:17580236/1‑137 (MQ=255)
                                                       tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCGATCTAGCCCCACACACGCGGCACACAAGCTGGAAGGAGCTGTAACACTGGGGGCGTACACACCGTca                                                                                  >  2:9626125/1‑137 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGCCAGATCGAGGCCCGCCCACGCGGCACACAATCTTGTAAACGCTGCACCATTTGAGGAGTCCACCCCGTcca                                                                                 >  2:4475544/1‑135 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCAAATCGAGCACCACCCCCAAGGAAAACACCCCGGAAAGCGATGGAACATTGGAGGCGTACAcccccccaa                                                                                 >  1:3259792/1‑132 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGCCCAACCGAACACCACCCCCACAGACCACCACCCTGCAGGAGCTGCACCCTTTGAGGAGTACAcccccccca                                                                                 >  2:8388393/1‑132 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCGCCCGCGCAGCACCCAGCCTTGTAGGCGCTGCCCTATTGGAGGTGTCCCCCCCATcac                                                                                 >  2:12206931/1‑137 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCAATCGAACACCACCCCCGCGGCACACAACCTGGCAGGCGATGCACTATTGGAGGCGTCCGCCCCTTcac                                                                                 >  2:12041331/1‑137 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCTCCCGACCGAGCCCCCCCCGCTCGGAACACGAGCTGGCAGGAGCTGCAACATTTGAGGAGTCCACCCCGTcac                                                                                 >  2:979886/1‑137 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCTCCCAATCGAGCACCGCCCGCGCGGCCCACGATCTGGCAAGGGATGCACCCTTTGAGGCATCCGCCCCGTcac                                                                                 >  2:12551539/1‑137 (MQ=255)
                                                        gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGGCAGATCTAGAACCGCCCACGCGGGACCAAAGCTGGGAAGAGATGCACTATTGGGGGAGTACACCCCATcaa                                                                                 >  2:18915311/1‑136 (MQ=255)
                                                        gggCCGAGAGCAACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCTAGATCGGGCACCATCCACGTGGAACACGATCTGGCAGGCGCCGGACTAATGGGGGATTCCACCCCGttaa                                                                                 >  2:10217730/1‑134 (MQ=255)
                                                          gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAGATCGAGCACCGCCCACGCGGGACACGACCTTGCAGGCGCTGCCCCCCTTGAGGCGTCCACCCCATccccc                                                                               >  2:3443526/1‑133 (MQ=255)
                                                          gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAATCGAGCACCACCCCCACGGCACACAATCCTGCAAGCGCTGCAACCCTTGAAGAGTACGCCCCATcacac                                                                               >  2:12997266/1‑136 (MQ=255)
                                                             gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTat                                                                            <  1:10483294/137‑1 (MQ=255)
                                                             gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGACCTAACCCCACCCCCGCTGCCCAAAACCCGGACGGCGCTGCACCATTGGAGGCGTACGCCCCATCACAttct                                                                            >  2:11128815/1‑135 (MQ=255)
                                                             gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCTAGCACCGCCCACGCAGCACACAACCTGGCCGGCGCTGCACTATTGGAGGCGTCCACCCCATCACACTat                                                                            >  2:6490009/1‑137 (MQ=255)
                                                             gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAAACCGAACACCGCCCAGACGGCACACGATCCTGAAAGAGATGCACTATTGGAGGAGTACGCCCCATCACACTat                                                                            >  2:12246805/1‑137 (MQ=255)
                                                                    actggagaAAGTCGGCTGGGGAGCATGAACGCCTTTGTACGTTGCGCCG‑CCTGCAAGATCGAGCACCACCCGCGCGGCACACGATCGGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACg                                                                     <  2:4028442/133‑1 (MQ=255)
                                                                       tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAAGCGAGCACCGCCCACGTGGCACACAATCTGGCAAGCACTGCACCATTGGAGGCGTACGCCCCGTCACATTCTCCTCCCGCCa                                                                  >  2:2461384/1‑137 (MQ=255)
                                                                              gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCCGCCAGATCGAGAAACACCCACGCGGCACAAGATCTGGCAGGTGCTGCACCCTTTGGGGCGTACGCCCCGTCCCATTCTCATCACGCCACTGTCgg                                                           >  2:4384972/1‑136 (MQ=255)
                                                                                 ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCTCCCA‑ATCGAGCACCGCCCGCGCGGCACACGACCTGGCAGGGGCTGCACTATTTGAGGCGTCCGCCCCGTCACATTATCCTCCCGCCCCTTTCGTAAt                                                        >  2:18985527/1‑137 (MQ=255)
                                                                                      gggCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCACCCGCGCGGAAAACGATCTGGCAGGAGCTGCACTACTGGAGGTATACGCCCCATCAAATTCGTATCACGACACCGTCGTAATCCGcc                                                   >  2:16298917/1‑137 (MQ=255)
                                                                                      gggCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCAATCGAGCAACACCCGCGCGGCACACAAGCTGGCGGGCATTGGACTATTTGAGGCGTACGCCCAGTAAAAGCATCATCACACCACCGTTGTAAtatccc                                                   >  2:8923722/1‑132 (MQ=255)
                                                                                                                 aGCG‑CCTGCCAGATTTAGGACCGCCCGCGCGGCACACAAGCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACACTATCATCACGCCACCGTCGTAATCTGCCGGACAAATCTATGCCACAAGGCACCCg                        >  2:13539625/1‑137 (MQ=255)
                                                                                                                 aGCG‑CCCGCCCAATCAAGCACCGCCCACGCGGCACACGAGCTGGCAGGCGCTTCACTCTTGGAAGCGTACGCCCCGCCACACTATCACCAAGCCACTGTCGTAATCTGCCGGCCAAACCTATGCCACAAGTCAccca                        >  2:15557564/1‑136 (MQ=255)
                                                                                                                   cg‑cCTGCCCGATCGAGCACCACCCGCGCGGCACACGATCTGGCAGGCGCTGCACCATTTGAGGCGTCCGCCCCGTCCCATTATCCTCCCGCCACTGTTGTAATCTTCCTGCCAACTCTCTGCCCCAGGTTACCCGGc                      >  2:7431177/1‑137 (MQ=255)
                                                                                                                   cg‑cCTGCCAGATCTAACACCGCCCACGCGGCACACGATCTTGCCAGCTCTTCCCCCTTTGAGGCGTACGCCCCGTCCCACTATCCTCACACCACTGTCGTAATCTGCCGGCCAACTCTCTGTCACAGGGCACCCGGc                      >  2:12877362/1‑137 (MQ=255)
                                                                                                                              ggtgAGCACTGCCAGGGGGACACCCAATCGGGCAGCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGcc            <  2:7855119/134‑1 (MQ=255)
                                                                                                                                     aCCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt     >  1:6041247/1‑137 (MQ=255)
                                                                                                                                       cgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                               >  2:1165421/1‑45 (MQ=255)
                                                                                                                                        gcccgcGCGGCACACGACCTGGCAGACGCTGCACTCCTAAAAGCATACGCCCCATCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCAACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt  >  1:5148525/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTT  >  AE015451_phaM1/4344173‑4344444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑