BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,344,312	0	G	A	47.8%	40.1 / 49.2	129	intergenic (+43/‑113)	PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base G (41/18); new base A (54/0); total (111/18)
Fisher's exact test for biased strand distribution p-value = 2.21e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

CGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTG  >  AE015451_phaM1/4344176‑4344448

cGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgcccg                                                                                                                                           <  1:4448396/137‑1 (MQ=255)

 gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCAccgcacgc                                                                                                                                          >  2:14397709/1‑137 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                       <  1:11462814/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                       <  2:14596591/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                       <  1:6339241/137‑1 (MQ=255)

    aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCcgcgcg                                                                                                                                       <  2:19308555/137‑1 (MQ=255)

     gCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCgg                                                                                                                                      <  1:15266618/137‑1 (MQ=255)

       gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGca                                                                                                                                    <  1:13300147/137‑1 (MQ=255)

       gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGca                                                                                                                                    <  2:4802907/137‑1 (MQ=255)

        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                                   <  1:10217772/137‑1 (MQ=255)

        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGcac                                                                                                                                   <  2:18152358/137‑1 (MQ=255)

        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGACCCAACACCACCCCCGCGGccc                                                                                                                                   >  1:8878601/1‑135 (MQ=255)

        tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAATCGAGCACCACCcacgcagaac                                                                                                                                   >  2:1094280/1‑132 (MQ=255)

          aaGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCacac                                                                                                                                 <  1:7394789/137‑1 (MQ=255)

           aGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCACATCGCGAACCACACACACGGAacaca                                                                                                                                >  1:7737169/1‑136 (MQ=255)

               gtgTTGGGCAGGGGCAGACTCTTGCATTCATACCAAATGGGCCGAGAGCGACCTGAGCAGGTCGGCCGGGGCGAATTAACGCTCTTGTACGTTGCAGCG‑CCTGCAGGATCGAGCACCGCCCGTGCGGCACACGA‑ttg                                                                                                                           <  2:17334888/135‑3 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCa                                                                                                                       >  2:13676142/1‑137 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAACCGAACACCACCCGCGCGGCACACAA‑TCTGGCa                                                                                                                       >  2:12236936/1‑137 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAGATCGAGCACCGCCCACGCGGCACACAA‑CCTggaa                                                                                                                       >  2:4177434/1‑135 (MQ=255)

                   tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAAACCTAGCACCCCCCACACGGCACACAA‑ACTGGCa                                                                                                                       >  2:15832203/1‑137 (MQ=255)

                    gggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCCATCGAGCACCACCCGCGCGGAACACAA‑TCTGGTAg                                                                                                                      >  1:15938601/1‑137 (MQ=255)

                        aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCTAGCACCACCCACGCAGCACACAA‑CCTGGCAGgcgc                                                                                                                  >  2:7925395/1‑137 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCAAATCGAACACCACCCACCCAGCACAAAA‑GCTGGCAAGGGATGCaa                                                                                                             >  1:9511904/1‑136 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGCCCGACCGAAACACACCCACGCAGCACACAA‑CCTGGCAGgcgatacaa                                                                                                             >  1:3338531/1‑131 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCCGATCGCGCACCACCCGCGCGGCACACAA‑TCTTGCAAGAGATGCAc                                                                                                             >  2:15476134/1‑137 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCAAGCACCGCCCACGCGGCACACGA‑TCTGGCAGGCGCCGCAc                                                                                                             >  1:18113452/1‑137 (MQ=255)

                             cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTTCCCGACCTAGCACCACCCACGCGGCACACGA‑TCTGGCAAGAGATGCaa                                                                                                             >  1:7623320/1‑136 (MQ=255)

                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGACCGAGCACCACCCACGCAGAACACGA‑CCCGGCAGGAGCCGGCCTAttt                                                                                                        >  2:16417501/1‑136 (MQ=255)

                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGAGCGAGAACCAACCGCGCGGAACAAAG‑TCTTGCAGGCGATGTACTCttt                                                                                                        >  2:9181479/1‑136 (MQ=255)

                                  gCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTGCCCGATCAAGGACCGCCCCCGCAGCACACAA‑CTTGGCAGGAGCCGCaacactg                                                                                                        >  1:14060210/1‑131 (MQ=255)

                                   cTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGCCAGATCGAGCACCACCCACACAGCACAAGA‑CATGGCAGGAGCTGCACTAtttg                                                                                                       >  2:7310338/1‑135 (MQ=255)

                                       cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGCCCGATCGAGAACCACCCACGCGGAACACAA‑CCTGGTAGGAACTGCACTCTTTGAGGc                                                                                                   >  2:12247616/1‑137 (MQ=255)

                                       cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTTCCAACCGAGCACCGCCCACGCGGAACACGA‑TCTGGTGGGCGCTGTACTATTGGAggg                                                                                                   >  1:8745101/1‑136 (MQ=255)

                                       cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTACCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                   <  1:19179795/137‑1 (MQ=255)

                                         tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATTGAGGACCCCCCCTACGGCACACGA‑GCCGGGCGGAGCTGTAATATTTGAGgtgt                                                                                                 >  2:14714352/1‑137 (MQ=255)

                                               ccGAATGGGCCGAGGGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATTGAGGACCGCCCGCGCGGCACAAGA‑GCTGGCAGGGGATGCAATATTGGAAGCGTACGacc                                                                                           >  2:18806299/1‑137 (MQ=255)

                                               ccGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAATCGAAAACCGCCCACGCAGAACACAA‑TCTGGCAGGCGCTGCACCCTTGGAGGCGTACGccc                                                                                           >  2:6712724/1‑137 (MQ=255)

                                                 gAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGAGTCCGCCCCg                                                                                         >  2:4243664/1‑137 (MQ=255)

                                                    tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCCGATCGAACACCACCCACGCAGAACACAA‑GCTGGCAGGAGTTTGACTCTTTGAGGAGTGCGCCCCTTcc                                                                                      >  1:12582197/1‑136 (MQ=255)

                                                    tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAATCCAGCACCCCCCACACGGCACACAA‑TCTGGCAGGAGCGGCACCATTGGAGGAGGACGCACCATca                                                                                      >  1:17580236/1‑137 (MQ=255)

                                                    tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAAGCGAGCCCCGACCACGCGGCACAATA‑TCCGGCAGGCAATGCAACACTGGAGGCGTACGccccatga                                                                                      >  2:10059403/1‑133 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGCCAGATCGAGGCCCGCCCACGCGGCACACAA‑TCTTGTAAACGCTGCACCATTTGAGGAGTCCACCCCGTcca                                                                                     >  2:4475544/1‑135 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCCACCCGACCGAAAACCACCCCCAAGGAACACAACTCT‑GTAGGAGATTCACTATTGGGGGGGTACGCCCCATcac                                                                                     >  1:12253824/1‑137 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCAAATCGAGCACCACCCCCAAGGAAAACAC‑CCCGGAAAGCGATGGAACATTGGAGGCGTACAcccccccaa                                                                                     >  1:3259792/1‑132 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAGATCGAGCACCGCCCACGCGGCACACAA‑TCTGGCAGGCGCTTCACTACTGGAGGCGTACACCCCGTcac                                                                                     >  2:12584915/1‑137 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCAATCGAACACCACCCCCGCGGCACACAA‑CCTGGCAGGCGATGCACTATTGGAGGCGTCCGCCCCTTcac                                                                                     >  2:12041331/1‑137 (MQ=255)

                                                     gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAATCGAACACCCCCCACGCGGTACCCAA‑GCCGGCCGGCGACGTACCATTGGGGGAGTACGCCCCCTccc                                                                                     >  2:16906667/1‑135 (MQ=255)

                                                       gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAGATCGAGCACCGCCCACGCGGGACACGA‑CCTTGCAGGCGCTGCCCCCCTTGAGGCGTCCACCCCATccccc                                                                                   >  2:3443526/1‑133 (MQ=255)

                                                       gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAATCGAGCACCACCCCCACGGCACACAA‑TCCTGCAAGCGCTGCAACCCTTGAAGAGTACGCCCCATcacac                                                                                   >  2:12997266/1‑136 (MQ=255)

                                                       gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACCGCCCGACCGAAAACCACCCCCACGGAACACAA‑CCTTGCCAGCGCTGCCCTCCTTGCGGGGTCCGCCCCATccccc                                                                                   >  2:4000274/1‑133 (MQ=255)

                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAAGCGAGCACCGCCCACGTGGCACACAA‑TCTGGCAAGCACTGCACCATTGGAGGCGTACGCCCCGTCACATTCTCCTCCCGCCa                                                                      >  2:2461384/1‑137 (MQ=255)

                                                                    tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CATGCCAGATCGAGCACCCCCCGCACGGCAAACGA‑TCTAGAAGGCGCTGCACTCTTGGAGGAATACGCCCCGCCACATCCTCATCACCCCa                                                                      >  2:12486362/1‑137 (MQ=255)

                                                                         aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAAATCGAGAACCACCCGCGCGGAACACGA‑TCTTGCAGGCGCTTCACTCTTGGAGGGGGAAGCCCCCTCACACTACCATTAAGCCaacgtc                                                                 >  2:12730890/1‑132 (MQ=255)

                                                                           gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCCGCCAGATCGAGAAACACCCACGCGGCACAAGA‑TCTGGCAGGTGCTGCACCCTTTGGGGCGTACGCCCCGTCCCATTCTCATCACGCCACTGTCgg                                                               >  2:4384972/1‑136 (MQ=255)

                                                                              ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCTCCCA‑ATCGAGCACCGCCCGCGCGGCACACGA‑CCTGGCAGGGGCTGCACTATTTGAGGCGTCCGCCCCGTCACATTATCCTCCCGCCCCTTTCGTAAt                                                            >  2:18985527/1‑137 (MQ=255)

                                                                              ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAGATCGAGCACCGCCCGCGCGGAACACGA‑TCTCGCAGGCGATGTACTCCTGGAGGCGTACGCCCCATCACATTGTCATCCCGCCACTGTAGTCAt                                                            >  2:8519397/1‑137 (MQ=255)

                                                                                   gggCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCACCCGCGCGGAAAACGA‑TCTGGCAGGAGCTGCACTACTGGAGGTATACGCCCCATCAAATTCGTATCACGACACCGTCGTAATCCGcc                                                       >  2:16298917/1‑137 (MQ=255)

                                                                                   gggCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCAATCGAGCAACACCCGCGCGGCACACAA‑GCTGGCGGGCATTGGACTATTTGAGGCGTACGCCCAGTAAAAGCATCATCACACCACCGTTGTAAtatccc                                                       >  2:8923722/1‑132 (MQ=255)

                                                                                     gcgcATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCCAATCGAGCACCACCCACGCAGCACACGA‑TCCTGCAGGCGCTGCACTACTGGAGGCATCCGCCCCCTCACATTACCCTCACACCACCTTCGTAATCTGCCgg                                                     >  2:62393/1‑137 (MQ=255)

                                                                                            aaCGCTCTTGTACGTTGCAGCG‑CCTGCCCGATTTAGCACCGCCCACGCGGCACACGA‑TCTGGCAGGTGTTTCACTGTTGGGGGCGTGCTCCCCGTTACATTGTTCTCACGCCACTGTTGTAATCTTTCGGCCAActc                                              >  2:18553135/1‑137 (MQ=255)

                                                                                                        cGTTGCAGCG‑CCTGCCAGATCGAATACCGCCCGCACGGCACACGA‑TCTTGGAGGAGATGCACTACTGGAGGCGTACGCCCCGTTACATTATCCTCCAAACACTGTCGTCATCTTCCGGCCAACCCTCTGTCACAGGt                                  >  2:9699832/1‑137 (MQ=255)

                                                                                                         gTTGCAGCG‑CCTGCCCGATCGAACCCCGCCCGCGCGGCCCACGA‑TCTGGCAGGCGCTGCACTATTGGAGGTGTACACCCCATCAACTCGTCACCAAGCCACTGTCGTAATCTGCCGGCCAAATCTCTGCCCCAGGtt                                 >  2:17631367/1‑136 (MQ=255)

                                                                                                              aGCG‑CCTGCCAGATTTAGGACCGCCCGCGCGGCACACAA‑GCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACACTATCATCACGCCACCGTCGTAATCTGCCGGACAAATCTATGCCACAAGGCACCCg                            >  2:13539625/1‑137 (MQ=255)

                                                                                                              aGCG‑CCCGCCCAATCAAGCACCGCCCACGCGGCACACGA‑GCTGGCAGGCGCTTCACTCTTGGAAGCGTACGCCCCGCCACACTATCACCAAGCCACTGTCGTAATCTGCCGGCCAAACCTATGCCACAAGTCAccca                            >  2:15557564/1‑136 (MQ=255)

                                                                                                                cg‑cCTGCCCGATCGAGCACCACCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACCATTTGAGGCGTCCGCCCCGTCCCATTATCCTCCCGCCACTGTTGTAATCTTCCTGCCAACTCTCTGCCCCAGGTTACCCGGc                          >  2:7431177/1‑137 (MQ=255)

                                                                                                                         cgATCGAGCACCGCCCGCGCGGGACACGA‑CCTTGCAGGGGCTGTACTCTTTGAGGAGTACGCCCCATCCCATTATCCCCACAACACCGTCTTCACCTGTCAGACAAACCTCTGCCACAGATTACCCCGCATTCTccc                  >  2:10012625/2‑136 (MQ=255)

                                                                                                                                  aCCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt         >  1:6041247/1‑137 (MQ=255)

                                                                                                                                  aCCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAAATCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt         >  2:5291736/1‑137 (MQ=255)

                                                                                                                                    cgcccgcGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                   >  2:1165421/1‑45 (MQ=255)

                                                                                                                                    cgcccgcGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                   <  1:1165421/45‑1 (MQ=255)

                                                                                                                                     gcccgcGCGGCACACGA‑CCTGGCAGACGCTGCACTCCTAAAAGCATACGCCCCATCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCAACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt      >  1:5148525/1‑137 (MQ=255)

                                                                                                                                       ccgcGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgc    >  1:17752441/1‑137 (MQ=255)

                                                                                                                                        cgcgcgGCACAGGC‑TTTGACCTCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   <  1:8820318/137‑1 (MQ=255)

                                                                                                                                        cgcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:17919287/1‑137 (MQ=255)

                                                                                                                                        cgcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:5565863/1‑137 (MQ=255)

                                                                                                                                        cgcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:18963903/1‑137 (MQ=255)

                                                                                                                                        cgcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:13043766/1‑137 (MQ=255)

                                                                                                                                        cgcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCCtgct   >  1:11978527/1‑137 (MQ=255)

                                                                                                                                        cgcgcgACACAAGC‑TTGGGCAGCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   <  2:2185396/137‑1 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCCCGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:8600312/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:13751614/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:14753145/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:11094946/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:17031445/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:5824822/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:13634837/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:889309/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:17941771/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:2193614/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:2761199/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:5673874/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  1:7023287/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:10310912/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:10667434/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:11303119/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:12141269/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:13507184/1‑137 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   <  1:16478211/136‑1 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct   >  2:16478211/1‑136 (MQ=255)

                                                                                                                                         gcgcgGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCAAGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg  >  2:2985388/1‑137 (MQ=255)

CGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGA‑TCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTG  >  AE015451_phaM1/4344176‑4344448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`