BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,344,323	0	G	A	27.5%	353.1 / 19.6	194	intergenic (+54/‑102)	PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base G (119/18); new base A (51/1); total (175/19)
Fisher's exact test for biased strand distribution p-value = 2.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 196 total aligned reads displayed.

GCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCT  >  AE015451_phaM1/4344191‑4344459

gtgTTGGGCAGGGGCAGACTCTTGCATTCATACCAAATGGGCCGAGAGCGACCTGAGCAGGTCGGCCGGGGCGAATTAACGCTCTTGTACGTTGCAGCG‑CCTGCAGGATCGAGCACCGCCCGTGCGGCACACGAttg                                                                                                                                      <  2:17334888/135‑3 (MQ=255)

    tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCa                                                                                                                                  >  2:13676142/1‑137 (MQ=255)

    tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAACCGAGCACCGCCCACGCGGAACACGATCTGGCa                                                                                                                                  >  2:16574424/1‑137 (MQ=255)

    tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAAACCTAGCACCCCCCACACGGCACACAAACTGGCa                                                                                                                                  >  2:15832203/1‑137 (MQ=255)

    tGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTGTCAGATCCAGCACCACCCACACGGCACACAACCCGGCa                                                                                                                                  >  1:6532100/1‑137 (MQ=255)

     gggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCCATCGAGCACCACCCGCGCGGAACACAATCTGGTAg                                                                                                                                 >  1:15938601/1‑137 (MQ=255)

     gggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCTTCCGATCGAGCACCACCCACACAGCACACCAACTggtaa                                                                                                                                 >  1:5678225/1‑134 (MQ=255)

         aGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAAGCTAACACCACCCACACGACACACAAACTGGCAggtaa                                                                                                                             >  1:12063917/1‑134 (MQ=255)

           tGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCCGATCGAGCCCCCCCCACACGGAACAAAATCTGGCAAGCGCTg                                                                                                                           >  2:3646741/1‑137 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGCCCGATCGAACACCTCCCCCGCGGCACAAAATCTGGCAGGCGCTGTAc                                                                                                                        >  1:17476134/1‑137 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCAAATCGAACACCACCCACCCAGCACAAAAGCTGGCAAGGGATGCaa                                                                                                                        >  1:9511904/1‑136 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGCCCGATTGCGCACCACCCGCGCGGCACACAAGCTGGCAGGCGATGCAc                                                                                                                        >  2:6193502/1‑137 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGCCCGACCGAAACACACCCACGCAGCACACAACCTGGCAGgcgatacaa                                                                                                                        >  1:3338531/1‑131 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCCGATCGCGCACCACCCGCGCGGCACACAATCTTGCAAGAGATGCAc                                                                                                                        >  2:15476134/1‑137 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGTCAGACCGAGCACCACCCGCGCGGAACACTATCTGGCAGGCGCTGCaa                                                                                                                        >  2:14907057/1‑136 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCACCCACACGGAACACAACCTGGCAAGTGCTGCAc                                                                                                                        >  1:4006749/1‑137 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCAATCGCGCCCCGCCCGAGCGGTACCAGATCTGGTAGGAGTTGCAc                                                                                                                        >  2:16425010/1‑137 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAATCGAGCACCACCCGCACGGCACACAATCTTGCAAGCGCttccc                                                                                                                        >  2:12314382/1‑133 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAATCGAACACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGccc                                                                                                                        >  1:18754832/1‑135 (MQ=255)

              cAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑ACTTCCCGACCTAGCACCACCCACGCGGCACACGATCTGGCAAGAGATGCaa                                                                                                                        >  1:7623320/1‑136 (MQ=255)

                    cTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGCCAGATCGAGCACCACCCACACAGCACAAGACATGGCAGGAGCTGCACTAtttg                                                                                                                  >  2:7310338/1‑135 (MQ=255)

                        cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTTCCAACCGAGCACCGCCCACGCGGAACACGATCTGGTGGGCGCTGTACTATTGGAggg                                                                                                              >  1:8745101/1‑136 (MQ=255)

                        cATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAACACCACCCGCGCGGCACACGACCTGGCAGGCGCTGCACTCTTTGAGGc                                                                                                              >  2:12069365/1‑137 (MQ=255)

                               aCCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCACCCACGCGGCACACAACCTGGCAAGAGCCGCACTACTGGAAGCGTACAcc                                                                                                       >  2:2947084/1‑137 (MQ=255)

                                ccGAATGGGCCGAGGGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATTGAGGACCGCCCGCGCGGCACAAGAGCTGGCAGGGGATGCAATATTGGAAGCGTACGacc                                                                                                      >  2:18806299/1‑137 (MQ=255)

                                     tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCCGATCGAACACCACCCACGCAGAACACAAGCTGGCAGGAGTTTGACTCTTTGAGGAGTGCGCCCCTTcc                                                                                                 >  1:12582197/1‑136 (MQ=255)

                                     tGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCGATCTAGCCCCACACACGCGGCACACAAGCTGGAAGGAGCTGTAACACTGGGGGCGTACACACCGTca                                                                                                 >  2:9626125/1‑137 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGCCAGATCGAGGCCCGCCCACGCGGCACACAATCTTGTAAACGCTGCACCATTTGAGGAGTCCACCCCGTcca                                                                                                >  2:4475544/1‑135 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGTCAAATCGAGCACCACCCCCAAGGAAAACACCCCGGAAAGCGATGGAACATTGGAGGCGTACAcccccccaa                                                                                                >  1:3259792/1‑132 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCTGCCCAACCGAACACCACCCCCACAGACCACCACCCTGCAGGAGCTGCACCCTTTGAGGAGTACAcccccccca                                                                                                >  2:8388393/1‑132 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCCGTCAGATCGAGAACCACCCCCACGGAAAACAAGCTTGTCAGAGCTGCACTACTTGAGGAGTCCGCCCCCTcac                                                                                                >  2:11932595/1‑137 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAGATCGAGCACCGCCCACGCGGCACACAATCTGGCAGGCGCTTCACTACTGGAGGCGTACACCCCGTcac                                                                                                >  2:12584915/1‑137 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCGCCCGCGCAGCACCCAGCCTTGTAGGCGCTGCCCTATTGGAGGTGTCCCCCCCATcac                                                                                                >  2:12206931/1‑137 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCTCCCGACCGAGCCCCCCCCGCTCGGAACACGAGCTGGCAGGAGCTGCAACATTTGAGGAGTCCACCCCGTcac                                                                                                >  2:979886/1‑137 (MQ=255)

                                      gggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCCAATCGAACACCCCCCACGCGGTACCCAAGCCGGCCGGCGACGTACCATTGGGGGAGTACGCCCCCTccc                                                                                                >  2:16906667/1‑135 (MQ=255)

                                      gggCCGAGAGCAACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCTAGATCGGGCACCATCCACGTGGAACACGATCTGGCAGGCGCCGGACTAATGGGGGATTCCACCCCGttaa                                                                                                >  2:10217730/1‑134 (MQ=255)

                                        gCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑TCTGTCCAATCAAGCACCACCCGCCCAGCACACAATCTTGCAGGCGATTCACCGTTTGAGGAGTACACCCCATcacac                                                                                              >  2:1163418/1‑136 (MQ=255)

                                           gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTTCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTat                                                                                           <  1:10483294/137‑1 (MQ=255)

                                           gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGACCTAACCCCACCCCCGCTGCCCAAAACCCGGACGGCGCTGCACCATTGGAGGCGTACGCCCCATCACAttct                                                                                           >  2:11128815/1‑135 (MQ=255)

                                           gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCTAGCACCGCCCACGCAGCACACAACCTGGCCGGCGCTGCACTATTGGAGGCGTCCACCCCATCACACTat                                                                                           >  2:6490009/1‑137 (MQ=255)

                                           gagaGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCCGCCAAACCGAACACCGCCCAGACGGCACACGATCCTGAAAGAGATGCACTATTGGAGGAGTACGCCCCATCACACTat                                                                                           >  2:12246805/1‑137 (MQ=255)

                                                  actggagaAAGTCGGCTGGGGAGCATGAACGCCTTTGTACGTTGCGCCG‑CCTGCAAGATCGAGCACCACCCGCGCGGCACACGATCGGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACg                                                                                    <  2:4028442/133‑1 (MQ=255)

                                                     tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CATGCCAGATCGAGCACCCCCCGCACGGCAAACGATCTAGAAGGCGCTGCACTCTTGGAGGAATACGCCCCGCCACATCCTCATCACCCCa                                                                                 >  2:12486362/1‑137 (MQ=255)

                                                            gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑GCCGCCAGATCGAGAAACACCCACGCGGCACAAGATCTGGCAGGTGCTGCACCCTTTGGGGCGTACGCCCCGTCCCATTCTCATCACGCCACTGTCgg                                                                          >  2:4384972/1‑136 (MQ=255)

                                                               ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGTCCTCCCA‑ATCGAGCACCGCCCGCGCGGCACACGACCTGGCAGGGGCTGCACTATTTGAGGCGTCCGCCCCGTCACATTATCCTCCCGCCCCTTTCGTAAt                                                                       >  2:18985527/1‑137 (MQ=255)

                                                                    gggCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCACCCGCGCGGAAAACGATCTGGCAGGAGCTGCACTACTGGAGGTATACGCCCCATCAAATTCGTATCACGACACCGTCGTAATCCGcc                                                                  >  2:16298917/1‑137 (MQ=255)

                                                                    gggCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCCGATCGAGCACCACCCACACGGCACACAACCTGGCAGGCGCCGCACCCCTTGAGGCGTCCGCCCCATCACATTCTCCCCCCACCACCTTCGTCATCTTcc                                                                  >  2:13027633/1‑137 (MQ=255)

                                                                             aaCGCTCTTGTACGTTGCAGCG‑CCTGCCCGATTTAGCACCGCCCACGCGGCACACGATCTGGCAGGTGTTTCACTGTTGGGGGCGTGCTCCCCGTTACATTGTTCTCACGCCACTGTTGTAATCTTTCGGCCAActc                                                         >  2:18553135/1‑137 (MQ=255)

                                                                                          gTTGCAGCG‑CCTGCCCGATCGAACCCCGCCCGCGCGGCCCACGATCTGGCAGGCGCTGCACTATTGGAGGTGTACACCCCATCAACTCGTCACCAAGCCACTGTCGTAATCTGCCGGCCAAATCTCTGCCCCAGGtt                                            >  2:17631367/1‑136 (MQ=255)

                                                                                               aGCG‑CCTGCCAGATTTAGGACCGCCCGCGCGGCACACAAGCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACACTATCATCACGCCACCGTCGTAATCTGCCGGACAAATCTATGCCACAAGGCACCCg                                       >  2:13539625/1‑137 (MQ=255)

                                                                                               aGCG‑CCCGCCCAATCAAGCACCGCCCACGCGGCACACGAGCTGGCAGGCGCTTCACTCTTGGAAGCGTACGCCCCGCCACACTATCACCAAGCCACTGTCGTAATCTGCCGGCCAAACCTATGCCACAAGTCAccca                                       >  2:15557564/1‑136 (MQ=255)

                                                                                                 cg‑cCTGCCCGATCGAGCACCACCCGCGCGGCACACGATCTGGCAGGCGCTGCACCATTTGAGGCGTCCGCCCCGTCCCATTATCCTCCCGCCACTGTTGTAATCTTCCTGCCAACTCTCTGCCCCAGGTTACCCGGc                                     >  2:7431177/1‑137 (MQ=255)

                                                                                                 cg‑cCTGCCAGATCTAACACCGCCCACGCGGCACACGATCTTGCCAGCTCTTCCCCCTTTGAGGCGTACGCCCCGTCCCACTATCCTCACACCACTGTCGTAATCTGCCGGCCAACTCTCTGTCACAGGGCACCCGGc                                     >  2:12877362/1‑137 (MQ=255)

                                                                                                          cgATCGAGCACCGCCCGCGCGGGACACGACCTTGCAGGGGCTGTACTCTTTGAGGAGTACGCCCCATCCCATTATCCCCACAACACCGTCTTCACCTGTCAGACAAACCTCTGCCACAGATTACCCCGCATTCTccc                             >  2:10012625/2‑136 (MQ=255)

                                                                                                               gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                        >  1:16497563/1‑137 (MQ=255)

                                                                                                                   aCCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAAATCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt                    >  2:5291736/1‑137 (MQ=255)

                                                                                                                    ccgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc                   >  1:5500511/1‑137 (MQ=255)

                                                                                                                     cgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                              <  1:1165421/45‑1 (MQ=255)

                                                                                                                      gcccgcGCGGCACACGACCTGGCAGACGCTGCACTCCTAAAAGCATACGCCCCATCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCAACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt                 >  1:5148525/1‑137 (MQ=255)

                                                                                                                        ccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgc               >  1:14671717/1‑137 (MQ=255)

                                                                                                                         cgcgcgGCACAGGCTTTGACCTCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct              <  1:8820318/137‑1 (MQ=255)

                                                                                                                         cgcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct              >  2:5565863/1‑137 (MQ=255)

                                                                                                                         cgcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct              >  2:14700327/1‑137 (MQ=255)

                                                                                                                         cgcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct              >  2:17919287/1‑137 (MQ=255)

                                                                                                                         cgcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct              >  2:18963903/1‑137 (MQ=255)

                                                                                                                         cgcgcgACACAAGCTTGGGCAGCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct              <  2:2185396/137‑1 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGTCGCTGCACTATTGGAGGCGTCCGCCCCGTCACATTATCATCCCGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCGCCCGGAATGCTCCGCCTGCTTGTCTtgctg             >  1:13532124/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:10667434/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:5824822/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:10387985/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  1:119429/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  1:2761199/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  1:11158953/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:4204762/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  1:2193614/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:3275022/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  1:13634872/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:8600313/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  1:1917053/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:12647436/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:13507184/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:889349/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  1:17941771/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             >  2:9058391/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTcgctg             >  1:14902767/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGCCTtgctg             >  1:13925606/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCATGCTTGTCTtgctg             >  2:8600270/1‑137 (MQ=255)

                                                                                                                          gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGACATGCTCCGCCTGCTTGTCTtgctg             >  1:17690968/1‑137 (MQ=255)

                                                                                                                          aagagacacaCGATCGGGCGGCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg             <  2:10065005/131‑1 (MQ=255)

                                                                                                                           cgcgTCACACGCTCTGGCGGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctgc            <  2:19143312/137‑1 (MQ=255)

                                                                                                                           agggacacacaTTCTGGCGTCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctgc            <  2:4321944/132‑1 (MQ=255)

                                                                                                                             cgacacaCGCTCGGGCAGCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTc          <  2:6787640/134‑1 (MQ=255)

                                                                                                                              ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa         >  2:7922812/1‑137 (MQ=255)

                                                                                                                              ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa         >  1:18079479/1‑137 (MQ=255)

                                                                                                                              ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa         >  1:17116615/1‑137 (MQ=255)

                                                                                                                              ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa         >  1:7369368/1‑137 (MQ=255)

                                                                                                                              ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa         >  1:10224782/1‑137 (MQ=255)

                                                                                                                              ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCATCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa         >  1:10741597/1‑137 (MQ=255)

                                                                                                                                 acacGTTCTGACACCCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCAccc      <  1:3501624/137‑1 (MQ=255)

                                                                                                                                   acGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct              >  2:2737722/1‑127 (MQ=255)

GCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCG‑CCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCT  >  AE015451_phaM1/4344191‑4344459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`