Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,889,6440GC30.2% 571.4 / 137.3 274intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
Reads supporting (aligned to +/- strand):  ref base G (139/34);  new base C (0/75);  total (139/135)
Fisher's exact test for biased strand distribution p-value = 3.48e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 275 total aligned reads displayed.

CCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTC  >  AE015451_phaM1/4889508‑4889779
                                                                                                                                         |                                                                                                                                       
ccacaGAAGAGGTCCTGGTTTGGCGGAAAACCCACAACCAAATCTGAGGAACACCTCGTGACTTTGTGGGAGCGGGCTCGCCCGCGAAAACCGGCAAGGCAGGGGCCACCCACCGAGTTGTT‑GGCTTCGTGGGCGAg                                                                                                                                         <  2:19352718/137‑1 (MQ=255)
         cGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGATCGACCCCGAACACCGGAAAAACCAGTGTCACCCACCGAGGTGTC‑TTTTTTGCGGGAGAGCCCACcccc                                                                                                                                >  2:14558101/1‑137 (MQ=255)
                     ggttaaaacCCCACAACGCAATCTGGGGAACAACTCGGGACCCTTGGGGAGCGGGCTCGCCCACGAACACTGACAAAGCTGGTTCCATCCACCGGTTTGTC‑TGCTTCGCGGGAGAGCCCGCTCCCACAGGGGGCCaa                                                                                                                    <  2:8322994/132‑1 (MQ=255)
                     ggccgAAAACCCAAAACCTAATCTGAAGAACACCTGGGGACCTGTTGGGAGCGGACTCTCCCGCGAAAACGTTCAAAGCCGGTGCCGTCCATTTGGTTGTC‑GGTTCCGCGGGAAACCCCGCTCCCACAGGGGGCCaa                                                                                                                    <  2:18260876/137‑1 (MQ=255)
                         gAAAACCCACAACGCAATCTGAGGAACAACTCGGGAACCTGTGGGAGCGGGGTCGCCCGCGAACCCCGGAAAAACCAGTGTCACCCCACAAATTGTC‑TTCTTTGCGGGAGAGGCCACTCCCACAAGGGGACaaaacg                                                                                                                >  2:9085146/1‑134 (MQ=255)
                                cacaACGCACTCTAAGAAACAATTGGTGCCCTTGGGGAAGTGGACTTTCCCACGAACACTGACAAACCCGGCGCCCTCAACGGATTGTTC‑TGCTTTGTGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTga                                                                                                         <  2:5745267/137‑1 (MQ=255)
                                  caACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCTAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgaga                                                                                                       <  2:17753214/137‑1 (MQ=255)
                                            ggagaAACAATTGGGGACCTTGTGGGAGGGGGCTCCCCCAAAAACACCGGAAAAGCCGGTGCCACCCACTGGGTTGTT‑TTTTTCGTGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAtt                                                                                             <  2:2425626/136‑1 (MQ=255)
                                                          gaaCCTTGTGGGAGCGGGCTCGCCCGAGAACACCGGAAAATCTGGTGCCATCCCCCGGTTTGTT‑TGTTTCGGGGGAAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAtt                                                                               <  2:3292147/135‑1 (MQ=255)
                                                               ttgtgGGGGTGGTCTCTCCCGAAAACACTGGCAAATCTGGTCCCACCCCCCGGTTTTTT‑TTTTTCGCGGACAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCa                                                                          <  1:11932446/136‑1 (MQ=255)
                                                               tggggggAGCGGGCTCGCCAGAAAACACAGGAAAATTTGGGGCCATCCACTGGTTTTTT‑TTTTTCGTGGGCGGTCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCa                                                                          <  1:17462340/133‑1 (MQ=255)
                                                                                        cacTGGCAAATCGGGTGCCGTCCACCGGGTTGTT‑GGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAc                                                 <  2:9168110/137‑1 (MQ=255)
                                                                                         acCAGCCAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCACGGGCGAGCCCGATCCCCAAGGGGGCCAAAGCTTGCTTGAGATATCACAATCCCAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAcc                                                >  1:4097029/1‑137 (MQ=255)
                                                                                            ggCAANGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                             >  1:12720701/1‑137 (MQ=255)
                                                                                            ggCAAATCCGGTGCCATCCACTGGGTGGTC‑TGTTTCGTGGGGGGTCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                             <  1:9670059/137‑1 (MQ=255)
                                                                                            ggCAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                             >  1:11732722/1‑137 (MQ=255)
                                                                                             gaaaaGCTGTTGCCATCCCTCGGGTTGTT‑GGCTTCGGGGGCGCCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGca                                            <  2:4211983/135‑1 (MQ=255)
                                                                                              cAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAGTCGGTTTCATCGGCACCGGcat                                           >  2:361037/1‑137 (MQ=255)
                                                                                              cAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                           >  1:6958265/1‑137 (MQ=255)
                                                                                              cAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                           >  2:13527238/1‑137 (MQ=255)
                                                                                              cAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcat                                           >  2:4529555/1‑137 (MQ=255)
                                                                                              cAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCAACGAcat                                           >  2:2988538/1‑137 (MQ=255)
                                                                                               aaaGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatc                                          <  2:10935586/137‑1 (MQ=255)
                                                                                                aaGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatca                                         >  1:3875555/1‑137 (MQ=255)
                                                                                                aaGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGc                                             <  1:15211903/133‑1 (MQ=255)
                                                                                                 ggCCGGTGCCATCCACCG‑GTTTTCTTGCTTCGTGGTCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatcat                                        <  2:14858875/136‑1 (MQ=255)
                                                                                                  gctggTGCCATCCACCGGGTTGTC‑TGTTTGGGGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                       <  2:1204803/134‑1 (MQ=255)
                                                                                                  gCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                       <  1:14432156/137‑1 (MQ=255)
                                                                                                  gCCGGTGCCATCCACCGAGTTGTC‑GGTTTTGCGGAAAAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                       <  2:12591770/137‑1 (MQ=255)
                                                                                                  cccGTTGCCATCCACCGAGTTGTC‑TGCTCCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                       <  2:17938192/136‑1 (MQ=255)
                                                                                                  cccGGTGCCACCCACCGAGTTGTC‑TGCTTCGCGGTCGGGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATg                                       <  2:18947128/136‑1 (MQ=255)
                                                                                                      gTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGTATCATGGGCa                                   >  2:2491801/1‑137 (MQ=255)
                                                                                                      gTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCAAAGGGGGCCAAAGCTTGCTTGAGATATCACAATTAAAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCa                                   >  2:7042501/1‑137 (MQ=255)
                                                                                                        gCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAg                                 >  1:14710480/1‑137 (MQ=255)
                                                                                                        gCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAg                                 >  2:12296311/1‑137 (MQ=255)
                                                                                                        gCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGACATCATGGGCAAg                                 >  1:247805/1‑137 (MQ=255)
                                                                                                        accATCCACCGGGTTTTT‑TGTTTGCTGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAg                                 <  2:2698583/136‑1 (MQ=255)
                                                                                                         ccacccacTGATTTTTC‑TGCTTCGCGGACAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                                <  1:12512116/137‑1 (MQ=255)
                                                                                                         ccATCCCCCGAGTTGTC‑TGCTTCGTGGTCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                                <  2:14810344/137‑1 (MQ=255)
                                                                                                         ccATCCACAGGTTGGTT‑TGTTTAGTGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc                                <  2:9819307/137‑1 (MQ=255)
                                                                                                          cgtcaaCCGATTTGTC‑GGTTTCGAGGGTGGCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                               <  2:13970464/132‑1 (MQ=255)
                                                                                                          cATCCCCGGGGTTGTC‑TGCTTCGCGGGCGAACCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                               <  2:13407310/137‑1 (MQ=255)
                                                                                                          cATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                               <  2:7691002/137‑1 (MQ=255)
                                                                                                          cATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                               >  2:7036355/1‑137 (MQ=255)
                                                                                                          cATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                               >  2:5563186/1‑137 (MQ=255)
                                                                                                          cATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGcc                               >  2:1939882/1‑137 (MQ=255)
                                                                                                               aCCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACCATTCCAAATAGGTAATTTCTCATGGCTACAATCGGTTTCATCGGCACCGGCCTCATGGGTAAGCCCATgg                          >  1:63228/1‑137 (MQ=255)
                                                                                                               aCCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                          >  1:5856484/1‑137 (MQ=255)
                                                                                                                ccGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGc                         >  2:7340219/1‑137 (MQ=255)
                                                                                                                    gTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                     >  1:13368044/1‑137 (MQ=255)
                                                                                                                    gTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                     >  2:13272056/1‑137 (MQ=255)
                                                                                                                    gTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                     >  2:17542586/1‑137 (MQ=255)
                                                                                                                    gTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg                     >  2:16125870/1‑137 (MQ=255)
                                                                                                                      tGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                   >  1:10419145/1‑137 (MQ=255)
                                                                                                                      tGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                   >  1:13218121/1‑137 (MQ=255)
                                                                                                                      tGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                   >  1:14186081/1‑137 (MQ=255)
                                                                                                                      tGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGaa                   >  2:1638348/1‑137 (MQ=255)
                                                                                                                         c‑TGCTTCTAGGGCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCt                <  1:4550180/137‑1 (MQ=255)
                                                                                                                         c‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCt                >  1:6620408/1‑137 (MQ=255)
                                                                                                                           tGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg               >  1:5970682/1‑137 (MQ=255)
                                                                                                                           tGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg               >  1:1913435/1‑137 (MQ=255)
                                                                                                                            gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGTCATCATGGGCAAGCCCATGGCTCAGAACCTGc              >  1:510530/1‑137 (MQ=255)
                                                                                                                            gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc              >  1:1071077/1‑137 (MQ=255)
                                                                                                                            gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc              >  2:6796168/1‑137 (MQ=255)
                                                                                                                            gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc              >  2:3972560/1‑137 (MQ=255)
                                                                                                                            gCTTCGCGGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAGTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGc              <  2:7579925/137‑1 (MQ=255)
                                                                                                                             ttccgcgGACAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCa             <  2:5327727/134‑1 (MQ=255)
                                                                                                                              ttCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaa            >  1:15859052/1‑137 (MQ=255)
                                                                                                                              ttCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaa            >  1:924579/1‑137 (MQ=255)
                                                                                                                              ttCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaa            >  2:8938206/1‑137 (MQ=255)
                                                                                                                              ttCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaa            >  2:15344823/1‑137 (MQ=255)
                                                                                                                               tagggggCAACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa           <  1:16748462/133‑1 (MQ=255)
                                                                                                                               tCGCGGTCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa           <  1:17244668/137‑1 (MQ=255)
                                                                                                                               tCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa           <  2:14391822/137‑1 (MQ=255)
                                                                                                                               tCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaa           <  2:18602312/137‑1 (MQ=255)
                                                                                                                                tgtgggCGCCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:7309158/134‑1 (MQ=255)
                                                                                                                                tgtgggCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  2:8843092/134‑1 (MQ=255)
                                                                                                                                tgcgGGCGCCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:11360593/136‑1 (MQ=255)
                                                                                                                                tgcgGACAAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  2:10518202/136‑1 (MQ=255)
                                                                                                                                tgagggCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCAATGGCTCAGAACCTGCaaaa          <  2:4769452/134‑1 (MQ=255)
                                                                                                                                ggagggAGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  2:15079537/134‑1 (MQ=255)
                                                                                                                                cgtgggCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:7955217/134‑1 (MQ=255)
                                                                                                                                cgtggccGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:8282693/131‑1 (MQ=255)
                                                                                                                                cgcgGGCGCCCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  2:18800974/137‑1 (MQ=255)
                                                                                                                                cgcgGGCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:13892596/137‑1 (MQ=255)
                                                                                                                                cgcgGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  2:9461751/137‑1 (MQ=255)
                                                                                                                                cgcgGCCGATCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  2:4459497/137‑1 (MQ=255)
                                                                                                                                cgcgAGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:10031534/137‑1 (MQ=255)
                                                                                                                                cacgGGCGACCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:6990803/135‑1 (MQ=255)
                                                                                                                                agggggAGAGCCAGATCCCACAGGGGGCCAAAGCGGGCTGGAGATATCAAAATTACAAAGAGGTAATTTCGCATGGCGAAAATCGGTTTCATCGGCACCGGCATCAGGGGCAAGCCCATGGCTCAGAACCTGCaaaa          <  1:46063/134‑1 (MQ=255)
                                                                                                                                  cgGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAgg        >  1:6381047/1‑137 (MQ=255)
                                                                                                                                   gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc       >  2:17026028/1‑137 (MQ=255)
                                                                                                                                   gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc       >  1:16889013/1‑137 (MQ=255)
                                                                                                                                   gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc       >  1:17809276/1‑137 (MQ=255)
                                                                                                                                   gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc       >  1:18572736/1‑137 (MQ=255)
                                                                                                                                   gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc       >  1:19510089/1‑137 (MQ=255)
                                                                                                                                   gggCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTCGAGATATCACAATTACAAAGAGGTAATTTCTAATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGc       >  2:3180858/1‑137 (MQ=255)
                                                                                                                                     gCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg     >  1:8637042/1‑137 (MQ=255)
                                                                                                                                      cGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAgg    <  1:15574785/137‑1 (MQ=255)
                                                                                                                                      cGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAgg    >  2:1425291/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTC‑TGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTC  >  AE015451_phaM1/4889508‑4889779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑